Conserved Protein Domain Family
AHBA_syn

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cd00616: AHBA_syn 
Click on image for an interactive view with Cn3D
3-amino-5-hydroxybenzoic acid synthase family (AHBA_syn). AHBA_syn family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The members of this CD are involved in various biosynthetic pathways for secondary metabolites. Some well studied proteins in this CD are AHBA_synthase, protein product of pleiotropic regulatory gene degT, Arnb aminotransferase and pilin glycosylation protein. The prototype of this family, the AHBA_synthase, is a dimeric PLP dependent enzyme. AHBA_syn is the terminal enzyme of 3-amino-5-hydroxybenzoic acid (AHBA) formation which is involved in the biosynthesis of ansamycin antibiotics, including rifamycin B. Some members of this CD are involved in 4-amino-6-deoxy-monosaccharide D-perosamine synthesis. Perosamine is an important element in the glycosylation of several cell products, such as antibiotics and lipopolysaccharides of gram-positive and gram-negative bacteria. The pilin glycosylation protein encoded by gene pglA, is a galactosyltransferase involved in pilin glycosylation. Additionally, this CD consists of ArnB (PmrH) aminotransferase, a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme. This CD also consists of several predicted pyridoxal phosphate-dependent enzymes apparently involved in regulation of cell wall biogenesis. The catalytic lysine which is present in all characterized PLP dependent enzymes is replaced by histidine in some members of this CD.
Statistics
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PSSM-Id: 99740
View PSSM: cd00616
Aligned: 87 rows
Threshold Bit Score: 284.048
Threshold Setting Gi: 29899155
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:1MDZ; catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1MDZ_A       22 ELAAVKTVLDsgwi-----ttgPKNQELEAAFCRLTG-NQYAVAVs-sATAGMHIALMALGig--eGDEVITPSMTWVST 92
gi 2599286   34 FFARLEWALNnnwl----tnggPLVREFEGRVADLAG-VRHCVATc-nATVALQLVLRASDv----SGEVVMPSMTFAAT 103
gi 29420391  18 FIPYLEIIWQnkqf----tnngPMHQKLEKKLCEFLG-VEYISLFn-nGTIALITAVQALGv----KGEVITTPYSFVAT 87
gi 29348294  15 SLEELNVYLQdiwqrkwitnngYYHQMLEAALCEYLG-VPYISLFt-nGTLPLTAALLAMRi----TGEVITTPFSFVAT 88
gi 15895617  15 YSDYVRKIFNsgiv----tnngEMVRKLKKSLEEYLG-VKNIVLVq-nGTLALQIAYKILNl----KKDIVTTPFSFVAT 84
gi 24374698  15 LNSYLERVHDtgwy----tnfgPLHNELTEKLEEYLG-VSNLLLVs-nGTLAIQLAAKVFN-----VKQTITTPFSFVAT 83
gi 2496480   51 LAEDFVQIAQanwy----tnfgPNERRFARALRDYLG-PHLHVATlanGTLALLAALHVSFgagtrDRYLLMPSFTFVGV 125
gi 30020083  17 YLDELKEIDTshiy----tnygPINQRFEETIMSSFFqNRGAVTTvanATLGLMAAIQLKKrr--kGKYALMPSFTFPAT 90
gi 24216296  19 WIEKVKVLSKnasf-----iggEEVSTLEKNLTSYTE-TKFSIACa-nGTDALQLALRALGvg--kGDSVILPDSTFWAT 89
gi 15606803  14 EKKALTEILEskri-----trdGWTDTFQEAFAEFLN-VKYCATVc-sGTVALFIGLKALGir--nGEKVVIPAMSFMAT 84
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1MDZ_A       93 LNMIVllGANPVMVDVDrdTLMVTPEHIEaai---tpqTKAIIPVHYAGAPADLDaiyal-geryGIPVIEDAAHATGts 168
gi 2599286  104 AHAASwlGLEPVFCDVDpeTGLLDPEHVAslv---tprTGAIIGVHLWGRPAPVEaleki-aaehQVKLFFDAAHALGct 179
gi 29420391  88 AHSLVlnGLKPVFVDIDskTLNIDPRRIEeai---tpeTQAIMPVHCYGNPCDTQaiadi-aqkyNLKVIYDAAHAFGve 163
gi 29348294  89 THSLWlnGIKPVFVDIDpvTCNLDPDKIEvai---tpnTTAIMPVHVYGYPCDTKsiqqi-adkyGLKVIYDAAHAFGve 164
gi 15895617  85 TSSQVweGLNPVFVDIDkkSLNIDTKLIEkni---tkrTSAIVPVHVFGNSCNIEeidkl-akryNLKVIYDAAHTFGvk 160
gi 24374698  84 SSSLLwqGIDLAYSDIErsSLNICPEELEknwk-sndsYDSLLATHVYGNPCDVQkidei-akkyNKKIMYDAAHAFGik 161
gi 2496480  126 AQAALwtGYRPWFIDIDanTWQPCVHSARavierfrdrIAGILLANVFGVGNPQIsvweelaaewELPIVLDSAAGFGst 205
gi 30020083  91 ALAAIwcGLEPYFIDISidDWYMDKTVLLdkieelkeeVAIVIPYATFGSWMNLEkyee--lekkGIPVVVDAAPGFGlm 168
gi 24216296  90 FESVVnvGADPYTVDTDpnDLQMDFVEFEkald--kikPKAAIIVHLYGWGSSRLedfrklckskGVPLLEDGAQCFGvk 167
gi 15606803  85 IDAVLlaGGIPVVVDVDe-YYTMDVNQLEdavk--kyrPRVVIPVHLYGQPADMEnimfl-adryGFFVLEDCAQAHGae 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            #                                                          
1MDZ_A      169 ykgr--higarGTAIFSFHAIKNI-TCAEGGIVVTDNPQFADKLRSLKFHGlgvdawdrqsggrapqaevlaPGYKYNLP 245
gi 2599286  180 aggr-pvgafgNAEVFSFHATKAV-TSFEGGAIVTDDGLLADRIRAMHNFGiapdk------------lvtdVGTNGKMS 245
gi 29420391 164 dddg-svlrhgDLSVLSFHATKVF-STFEGGAIVCNSKEMKEKIDRLKNFGyidet------------niniIGSNGKMS 229
gi 29348294 165 rege-silnagDMSTLSFHATKTF-NTIEGGALVLHDEHTKKRVDYLKNFGfvget------------evviPGTNGKLD 230
gi 15895617 161 ykdk-nilsygDISIISFHATKIF-HTIEGGALVINDDKLYEKAILSRNFGiesad------------sikdLGINAKMN 226
gi 24374698 162 vgnk-svlsfgDASTLSFHATKVF-HTVEGGAVIFKDRNHFDEAKSLINFGiqndg------------tlgtPGINAKLN 227
gi 2496480  206 yadgerlggrgACEIFSFHATKPF-AVGEGGALVSRDPRLVEHAYKFQNFGlvqtr------------esiqLGMNGKLS 272
gi 30020083 169 nggmhygqdfsGMIVYSFHATKPF-GIGEGGLIYSKNEEDIQHIKRMGNFGfdknr------------ectmMGFNCKMS 235
gi 24216296 168 fkga-siykdaLISTTSFYPAKVLgGAGDGGAVFTNDETLANKVKMLANHGrishy------------aygdVGWNSRLD 234
gi 15606803 161 fkgk-kvgawgHLSAFSFYASKNV-PMGEGGALTTNDESLFKEVKKWIDFGe-------------------hPAFNVKIT 219
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1MDZ_A      246 DLNAAIAlAQLQKLDALNARRAAIAAQYHQAMad---lpFQPLSLPswe-hiHAWHLFIIRvdear--cgitRDALMASL 319
gi 2599286  246 ECAAAMGlTSLDAFAETRVHNRLNHALYSDELrdv--rgISVHAFDp----gEQNNYQYVIisvdsaatgidRDQLQAIL 319
gi 29420391 230 EVNAAFGlLQLEHMDTFLRGRMNADMFYRQKLkditgisIVIPSGQk----iSNFSYFPILvesd---fplsRDELFNYL 302
gi 29348294 231 EVRAAYGlLNLKQVDAAIEARCQATTNYREALrnv--sgITFMDDIsg--vkHNYSYFPIFvdeer--ygmsRDELYFKM 304
gi 15895617 227 EFEAAMGlCLLDDIDKIFERRKKVYENYMNYIkg----dIYFQSRSrd--ctCNYGYFPIIfkn-----eniLKRVMKEL 295
gi 24374698 228 EYQAAVGlCLLESIDDIICHRSNLFEIYRKELsn----vIELPLWHr----dSNYNGAYMPilps---teveRLKIGEQL 296
gi 2496480  273 EISAAIGlRQLVGLDRRLASRRKVLECYRTGMad---agVRFQDNAn----vASLCFASACcts-----adhKAAVLGSL 340
gi 30020083 236 EYAAAIGiATIKKWDQKLKERSRISEWYKQLLqn---tgLMKKGWKvqkteaVIHQFMPIIcpe-----evrNIQVIEEL 307
gi 24216296 235 TLQAAFLnLNLKHLEVRIASRLKSVQKYYDILpn---lgIQVIHPPk----dYEENGYCNVtlst----peeRPMIQEVL 303
gi 15606803 220 EFQAAIGtLQLRKLKERNERRRNIARIYTENLkg----lCEVPEEReg--ayHVYHLYTLRhk--------eRDRLVEHL 285
                       330       340       350       360       370       380
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                                        
1MDZ_A      320 KTKgigTGLHFRAAHtqkyyre----rfptltlPDTEWNSERICSLPLfpdMTESDFDRVITALH 380
gi 2599286  320 RAEkvvAQPYFSPGChqmqpyr----tepplrlENTEQLSDRVLALPTgpaVSSEDIRRVCDIIR 380
gi 29420391 303 KNQnifARRYFYPVIpdfqayln---vgevcdvKNAREIASKVLCLPMhaeLSSDILEYIVSTIR 364
gi 29348294 305 KEYnvlGRRYFYPLIstfstyrel-ksarkenlPVATKMAEQVICLPMhhaLSEDDMERVLRLIR 368
gi 15895617 296 INEgvrPRRYFYPCLndlsyi------kdyrkvPVAQDISKRILCLPLydsLNITEQNKIINIVN 354
gi 24374698 297 KANniqFRRYFYPSLdkaylr------vksfncPVSNYIAENALCLPLhmyMTKEDVLKITATIK 355
gi 2496480  341 RRHaieARDYYNPPQhrhpyfvtnaelvestdlAVTADICSRIVSLPVhdhMAPDDVARVVAAVQ 405
gi 30020083 308 KKQkieARLYFSPSChqqvlfk----nykstdlTKTNKIAKRIVSLPLwegMTKELVEQIVICLE 368
gi 24216296 304 KEKeigFGNIYPGAMsdqpgakpy-ikgkfgekHITGRICSSVLNYPLfpyMKEEELEEVFSAIR 367
gi 15606803 286 RSKgidARVYYTYLLhelrna-------ealptPKAERFKREVFSIPVhpfLKEEEVLYIIESLK 343

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