Conserved Protein Domain Family
Tnase_like

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cd00617: Tnase_like 
Click on image for an interactive view with Cn3D
Tryptophanase family (Tnase). This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD correspond to tryptophanase (Tnase) and tyrosine phenol-lyase (TPL). Tnase and TPL are active as tetramers and catalyze beta-elimination reactions. Tnase catalyzes degradation of L-tryptophan to yield indole, pyruvate and ammonia and TPL catalyzes degradation of L-tyrosine to yield phenol, pyruvate and ammonia.
Statistics
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PSSM-Id: 99741
View PSSM: cd00617
Aligned: 9 rows
Threshold Bit Score: 663.671
Threshold Setting Gi: 20140926
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Comment:PLP cofactor required for activity
  • Structure:2TPL_A; Citrobacter tyrosine phenol-lyase
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2TPL_B       23 ERLKKMQeagYNTFLLNSKDIYIDLLTDSGTnaMSDKQWAGMMMGDEAyaGSENFYHLERTVqel--------------- 87
1AX4_A       24 EREAALKeagYNPFLLPSSAVYIDLLTDSGTnaMSDHQWAAMITGDEAyaGSRNYYDLKDKAkel--------------- 88
gi 31563036  26 EREIAMKeagYNTFNLRSEDVYIDLLTDSGTnaMSDAQWAGMMIGDEAyaGSKNWLFLESTIkel--------------- 90
gi 23127778  34 EREQAIAqagYNTFLLRSEDVYIDLLTDSGTsaMSDYQWAGMMLGDEAyaGSKNFYNLEASIqky--------------- 98
gi 29346902  24 EREQWIKeahYNVFQLKSEQVYIDLITDSGTgaMSDRQWAGMMLGDESyaGATSFFKLKDTItri--------------- 88
gi 20140425  23 ERERLARaagYNLFGLHSDDVLIDLLTDSGTgaMSSLQWAAVMQGDESyaGSPSFFRFEAAVqnl--------------- 87
gi 20140926  29 ERLRRLReagWNVFRLRSIDIFIDLLTDSGTgsMSIYQWAALMTGDEAyaGARSWFRFRDAVrdv--------------- 93
gi 20140810  19 DREAALAdagHNVFELASDDVAVDLLTDSGTgtMSNDQWAAMLQGDEAyaGSASFEDLAVAAedv--------------- 83
gi 19705248  28 QRERALKathYNEFAFPAGMLTVDMLSDSGTtaMTDQQWSAMFLGDESygRNKGYYVLLDAMrdcfergdnqkkiinlvr 107
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2TPL_B       88 -----------------------------fgFKHIVPTHQGRGAENLLSQLAIkp--------gqYVAGNMYFtttrYHQ 130
1AX4_A       89 -----------------------------fnYDYIIPAHQGRGAENILFPVLLkykqkegkaknpVFISNFHFdttaAHV 139
gi 31563036  91 -----------------------------fgFKHVVPTHQGRGAENLLSSIAIkp--------gqYVAGNMYFtttrYHQ 133
gi 23127778  99 -----------------------------ygYRHIVPTHQGRGAENILSQILIkp--------gdYIPGNMYFtttrLHQ 141
gi 29346902  89 -----------------------------tgFEYIIPTHQGRAAENVLFSYLVhe--------gnIVPGNSHFdttkGHI 131
gi 20140425  88 -----------------------------mpFKHIIPTHQGRAAEAILFSIFGgk--------grRIPSNTHFdttrGNI 130
gi 20140926  94 -----------------------------lgLDLVLPVHQGRAAERILYGELLrrr------narIVPANTHFdtgrAVI 138
gi 20140810  84 -----------------------------mgFQHIIPAHQGRGAENVLYGAVLsa--------gdTVLNNAHFdttrAHV 126
gi 19705248 108 tdcqdiekmmnemylceyegglfnggaaqleRPNAFLMPQGRAAESILFEIVRkilavrepgkvfTIPSNGHFdtteGNI 187
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2TPL_B      131 EKnGAVFVDIvrdeahd------aglniafKGDIDLKKLQKLIdekgaenIAYICLAVTVnlagGQPVSMANMRAVRELT 204
1AX4_A      140 ELnGCKAINIvtekafd------setyddwKGDFDIKKLKENIaqhgadnIVAIVSTVTCnsagGQPVSMSNLKEVYEIA 213
gi 31563036 134 EKnGGIFVDIirdeahd------asidipfKGNIDVNKLEKLIeekgaenIAYVCLAVTVnlagGQPVSMANMRAVRELT 207
gi 23127778 142 ELaGGTFVDViideahd------aqslhpfKGNVDLQKLTDLIervgaerIPYISVAGTVnmagGQPISMANLRAVHQLA 215
gi 29346902 132 EGrHAIALDCtideakn------tqleipfKGNVDPAKLEKALsey-adrIPFIIVTITNntagGQPVSMQNLREVRAIA 204
gi 20140425 131 EAsGATGDDLviaegkd------pqnlhpfKGNMDLARLEAYLeah-haeVPLVMITITNnaggGQPVSLANIRAVADLA 203
gi 20140926 139 LNqGGVPLDLpspqasr-------reaypfKGDIDVARLERLLker-srdVAFILLVITNntagGQPVSMDNVKTVRELA 210
gi 20140810 127 AAnDATPVDCpvdgard------pdtdapfKGNFSVERARRVVddvgadaVPVVVLTITNnsmaGQPVSIENTREVAAFA 200
gi 19705248 188 KQmGSVPRNLynkellyevpeggryeknpfKGDMDINKLQQLIdavgienIPMIYTTVTNnticGQAVSMKSIRETAKIA 267
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                           #                           
2TPL_B      205 AAHGIKVFYDATRCVENAYfikeqeqgfenksiaeIVHEMFSYADGCTMSGKXDCLVNIGGFLCMNdd-eMFSSAKELVV 283
1AX4_A      214 KQHGIFVVMDSARFCENAYfikardpkyknatikeVIFDMYKYADALTMSAKXDPLLNIGGLVAIRdneeIFTLARQRCV 293
gi 31563036 208 AKHGIKVFYDATRCVENAYfikeqeegyadvsikdIVHEMFSYSDGATMSGKKDGIVNIGGFLAINde-eLFGKAKEIVV 286
gi 23127778 216 QTYGIRIILDATRAVENAHfiqqreedyssqaiatILREFCSYTDGCTMSGKKDALVNIGGWLALNdy-nLYEEARNLIV 294
gi 29346902 205 NKYNKPVLFDSARFAENAYfikmrekgyqdksikeITREMFDLADGMTMSAKKDGIVNMGGFIATRrk-eWYEGAKGFCV 283
gi 20140425 204 HRYGKPFVIDGCRFAENAWfiktreegqadrsipeIVRDCFAVADGMTMSAKKDAFGNIGGWLALNdd-dLAEEARGHLI 282
gi 20140926 211 DAYGLPLVMDICRFAENAYlvkerdpryrgwsvrdIAREMISYGDHFVMSAKKDGLANIGGFIATRdp-sLYEDLAARVV 289
gi 20140810 201 DDIDATFVIDACRFAENAHfvqqrepgyehdsvaaIAREQLSYADACVMSGKKDGLVNVGGFVGLHddgrLHEQCRQRGI 280
gi 19705248 268 HKYEIPFMLDAARWAENCYfikmneegyrdksiaeIAKEMFSYCDGFTASLKKDGHANMGGILAFRd--kGYFWKKFSDF 345
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2TPL_B      284 VYEGmpsy-------------------gglaGRDMEAMAIGLREAMqyeyIEHRVKQVRYLGdKLKAAGVPiv-ePVGGH 343
1AX4_A      294 PMEGfvty-------------------gglaGRDMAAMVQGLEEGTeeeyLHYRIGQVKYLGdRLREAGIPiq-yPTGGH 353
gi 31563036 287 VYEGmpsy-------------------ggmtGRDMQAIAIGFREAMqyeyIEHRIRQVRYLGdRLKEAGVPiv-ePVGGH 346
gi 23127778 295 IYEGlhty-------------------ggmaGRDMEAMARGIEESVqddhIRARVGQVEYLGqKLLDWGIPiv-vPIGGH 354
gi 29346902 284 QYEGylty-------------------ggmnGRDMNALAIGLDENTefdnLETRIKQVEYLAqKLDEYEIPyq-rPAGGH 343
gi 20140425 283 RTEGfpty-------------------gglaGRDLDALAQGLVEIVdedyLRYRIRTHQYIVeRLDAMGVPvv-kPAGGH 342
gi 20140926 290 LEEGyvty-------------------gglaGRDLEAIAQGLREVVeedyLRHRVEQVRYLGeLLSSQGVPiv-ePVGGH 349
gi 20140810 281 LYEGfsty-------------------ggmsGRDMAAFAVGLREAVeppyVAERVAQVQRLAdALTDRDVPiy-qPAGGH 340
gi 19705248 346 NEDGtvktdvgillkvkqissygndsygsmsGRDIMALAAGLYECCnfnyLQERVEQCNYLAeGFYKAGVKgvvlPAGGH 425
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2TPL_B      344 AVFLDArrfceh-ltqdefPAQSLAASIYvetGVRSMERGiisagrnnvtgehhrpkletVRLTIPRrvYTYAHMDVVAD 422
1AX4_A      354 AVFVDCkklvpq-ipgdqfPAQAVINALYlesGVRAVEIGsfllgrdpatgeqkhadmefMRLTIARrvYTNDHMDYIAD 432
gi 31563036 347 AVFLDArrfcph-ldqeqfPAQSLAASLYidsGVRSMERGivsagrdvntgenhkpkletVRLTIPRrvYTYKHMDVVAE 425
gi 23127778 355 AIYLDAkrflpq-ipqdqfPAQRLAAELYleaGIRAMERGivsagrnketgdnyypelelVRLTIPRrvYTQAHMDLTAE 433
gi 29346902 344 AIFVDAskvlth-vpkeefPAQTLTVELYleaGIRGCEIGyiladrdpithenrfngldlLRLAIPRrvYTDNHMNVIAA 422
gi 20140425 343 AVFIDArawlsh-ippleyPGQALAVALYeiaGVRSCEIGtamfgrqpdg-sekpaamdlVRLAFPRrtYTQSHADYIVE 420
gi 20140926 350 AVYVDVlealpe-mprshyPADALAAALYlesGVRAVGLGalafareeng-eivypefelLRLAVPRrtYTNSHMEYVAA 427
gi 20140810 341 AVYIDAnaalph-lpreqfPGQAFVCELYregGVRAVELGrfafpd--------tdrrdlVRLALPRrtYGPDHLDHVAD 411
gi 19705248 426 GVYINMdeffdgkrghetfAGEGFSLELIrryGIRVSELGdysmeydlktpeqqaevanvVRFAINRsvYSQEHLDYVIA 505
                       490       500       510
                ....*....|....*....|....*....|....
Feature 1                                         
2TPL_B      423 GIIKLyqh-kedIRGLKFIYEPk--qLRFFTARF 453
1AX4_A      433 ALIGLkek-fatLKGLEFEYEPp--vLRHFTARL 463
gi 31563036 426 SVIHLykh-kedIRPLKFTYEPa--qLRFFTAKF 456
gi 23127778 434 AVEEVyhn-rdrLRGLKMIYEPk--yLRFFQARF 464
gi 29346902 423 ALRNVferretiTRGVRIAWEAp--lMRHFTVQL 454
gi 20140425 421 AFEELaat-kdaLRGYRIVKEPk--lMRHFTCRF 451
gi 20140926 428 SLARLlregrrkVKGLRVVKEPrikgIRHFLAEL 461
gi 20140810 412 TAAAVcer-gtdVTGLEIVSEPelteLRHFSAAL 444
gi 19705248 506 AVKALyed-resIPNMRIVSGHnl-pMRHFHAFL 537

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