5MDH,1BMD,1CIV,7MDH


Conserved Protein Domain Family
MDH

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cd00704: MDH 
Click on image for an interactive view with Cn3D
Malate dehydrogenase
Malate dehydrogenase (MDH) is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. MDHs belong to the NAD-dependent, lactate dehydrogenase (LDH)-like, 2-hydroxycarboxylate dehydrogenase family, which also includes the GH4 family of glycoside hydrolases. They are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133420
View PSSM: cd00704
Aligned: 17 rows
Threshold Bit Score: 438.63
Threshold Setting Gi: 56971399
Created: 7-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              # ####                         ##                                         
5MDH_A         4 IRVLVTGAAGQIAYSLLYSIGNGSVFgkdqpIILVLLDITpmmgVLDGVLMELQDCALPllkDVIATDKEEIAFKDLDVA 83
gi 78192396    4 VKVAVTGAAGQIGYALVPLIARGILLgptthVELRLLDIEpalkALAGVEAELDDCAFPlldKVVITADPRVAFDGVSIS 83
gi 15077032    6 KRVAVTGAAGQIGYSLLPLIAAGRMLgfdqrVQLQLLDISpalkALEGIRAELMDCSFPlldGVVITDEPKVAFDKADIA 85
gi 27733395   25 LHVLVTGAAGQIGYNLLFLIAHGLMFgpnqtVYLHLYDIMv--eAMEGVKMELADCCFPlvkGVVASNKTEVAFKDVECA 102
1BMD_A         5 VRVAVTGAAGQIGYSLLFRIAAGEMLgkdqpVILQLLEIPqamkALEGVVMELEDCAFPllaGLEATDDPDVAFKDADYA 84
gi 209586343   8 TRVAISGAAGNIGYALLPLLASGYVFgddrsVELRLLEIPhavkALAGVRMELIDCAFPcltDVIITTEPEEAFEGADVI 87
gi 5531799     6 LRVCISGAAGQICYSVLFRIAAGDMLgydqpVHIVMLEVPaalkAAEGVAMELVDCAFPllsGFTLTSDNAEAFKDVDYC 85
gi 167537489   8 LTVCVTGAAGYTAYNLVYLIASGEVFghdqpVALRLLDIPllqaKMEGVQMELQDCAFRllkDVSTHTSPDAAFKNVDYA 87
1CIV_A        43 INVAVSGAAGMISNHLLFKLASGEVFgpdqpISLKLLGSErsfaALEGVAMELEDSLYPllrQVSIGIDPYEIFQDAEWA 122
7MDH_B        33 VNIAVSGAAGMISNHLLFKLASGEVFgqdqpIALKLLGSErsfqALEGVAMELEDSLYPllrEVSIGIDPYEVFEDVDWA 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          ###                                              ###                        # 
5MDH_A        84 ILVGSMPRRDGM-------ERKDLLKANVKIFKCQGAALDKYAKkSVKVIVVGNPANTNCLTASKSAps-ipKENFSCLT 155
gi 78192396   84 IMCGAFPRRAGM-------ERKDLLEMNARIFKEQGEAIAAVAApDCRVLVVGNPANTNALILLKSAqgklnPRHVTAMT 156
gi 15077032   86 ILCGAFPRKPGM-------ERRDLLQTNAKIFSEQGRVLGEVASpNCRVCVVGNPANTNALILLRESkgklnPRFVTALT 158
gi 27733395  103 ILVAGMPRKVGM-------ERKELIGINTRIMKEQALALKNFANpHVRVLVVANPANTNALVVANNAgi--dVKQITCLT 173
1BMD_A        85 LLVGAAPRKAGM-------ERRDLLQVNGKIFTEQGRALAEVAKkDVKVLVVGNPANTNALIAYKNApg-lnPRNFTAMT 156
gi 209586343  88 VLVGGFPRKQGM-------ERKDLIHANTKIFTTMGRAIEEVASpNVKVLVVANPANTNCLVALNEAsr-ipSKNFCALT 159
gi 5531799    86 LLFGAFPRKAGM-------ERAELLSKNKGIFQIQGAAINEHAKpTCRILVIGNPANTNALVLSTQLtk-ipKTNVTAMS 157
gi 167537489  88 ILLSAIPRSEDAdpmlrcnNRLDVLRANTVIFKEHGLAFDRVAKpTTKVVVVANPSATNALIVSSFAks-vpKENFTSLA 166
1CIV_A       123 LLIGAKPRGPGM-------ERADLLDINGQIFAEQGKALNAVASpNVKVMVVGNPCNTNALICLKNApn-ipPKNFHALT 194
7MDH_B       113 LLIGAKPRGPGM-------ERAALLDINGQIFADQGKALNAVASkNVKVLVVGNPCNTNALICLKNApd-ipAKNFHALT 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                 #                                      
5MDH_A       156 rLDHNRAKAQIALKL-----------GVTSDDVKnVIIWGNHSSTQYPDVNHAKvklqakevg---vyeavkddSWLKGE 221
gi 78192396  157 rLDHNRALSLLARKA-----------GVPVSQVRnVIIWGNHSSTQVPDIDNAVigttpa--------reaikdGALNDD 217
gi 15077032  159 rLDHNRATAQVAERA-----------RARVEEVKnCIIWGNHSGTQVPDVNSATvggkpar-------aavdndAFFDNE 220
gi 27733395  174 rLDQNRAIAQIASKL-----------NCKVEDVSdAFVWGNHSEKQCPDISHAVvqtpngpk-----rvadlveESWLES 237
1BMD_A       157 rLDHNRAKAQLAKKT-----------GTGVDRIRrMTVWGNHSSTMFPDLFHAEvdgrpa--------lelvdmEWYEKV 217
gi 209586343 160 yLDHQRAKAQVAIRL-----------GVRPNQVKnVSIWGNHSNTQYPDVLTDGyisfdsgedipistlmandlEWTNDD 228
gi 5531799   158 rLDHNRAVGQVAGKL-----------GVRTNRISnVWVAGNHSNTMVPIVDCGViydeglkdk--qpvkpmlpaDWIKGE 224
gi 167537489 167 wLDQNRAAAHIAARLnedklestvplQHTGRDIRnLIVWGNHSDTMFPDTRQAVivqpkpelppvklravlrddGFLLKD 246
1CIV_A       195 rLDENRAKCQLALKA-----------GVFYDKVSnVTIWGNHSTTQVPDFLNAKihgipvt-------evirdrKWLEDE 256
7MDH_B       185 rLDENRAKCQLALKA-----------GVFYDKVSnVTIWGNHSTTQVPDFLNAKidgrpvk-------evikrtKWLEEE 246
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
5MDH_A       222 FITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTpe-gEFVSMGIISDGnsy-gvPDDLLYSFPVTIKDKTw-kI 298
gi 78192396  218 FVQLVRERGAEIIQLRGLSSAMSAAKAAVDHVHDWIHGTpe-gVYVSMGVYSDGnpy-gvPSDLIFSFPCTCHAGEw-tV 294
gi 15077032  221 FITIVQERGAEIMKLRGLSSALSAAKAIVDHVHDWMLGTps-gTHVSMAVYSDGnpy-gvPGGLIFSFPVTCSGGEw-qI 297
gi 27733395  238 FNKTVQERGAKVIEMRKASSAASAAKAIVDHFRDWCLGTnk-eKIVCMGVYSEGqy--giPKGIFFSMPVVCYGGEy-hI 313
1BMD_A       218 FIPTVAQRGAAIIQARGASSAASAANAAIEHIRDWALGTpe-gDWVSMAVPSQGey--giPEGIVYSFPVTAKDGAy-rV 293
gi 209586343 229 FVQIVQNRGKHVIEVRGNSSALSAAQATADCLATWLVTGtkrgETISMAVYNDKgyy-gvKKGLVFSFPCECRDGDw-fV 306
gi 5531799   225 FVPCVRGRGTAVIEARGHSSSASAANAAIDHVRDWHMGTkp-gAFVSCSLLIEEgnpygiAPGIFYSMPCSCVGGKw-qI 302
gi 167537489 247 LVDHVRQRGKAIVRKRQTTPAMSAARAVADHVCALHGGTre-gETVSMGVYTNGnpy-qvADGLFFSFPVKIKAGGewhI 324
1CIV_A       257 FTNMVQTRGGVLIKKWGRSSAASTAVSIVDAIRSLVTPTpe-gDWFSTGVYTNGnpy-giAEDIVFSMPCRSKGDGdyeF 334
7MDH_B       247 FTITVQKRGGALIQKWGRSSAASTAVSIADAIKSLVTPTpe-gDWFSTGVYTTGnpy-giAEDIVFSMPCRSKGDGdyeL 324
                        330       340
                 ....*....|....*....|....*....
Feature 1                                     
5MDH_A       299 VEGLPINdFSREKMDLTAKELAEEKETAF 327
gi 78192396  295 VSDKLNGdLGKQRLASTIAELQEERAQAG 323
gi 15077032  298 VSGLNVTpAISERIKATTTELEEERREVS 326
gi 27733395  314 VDDLVLSeSTRTMLKNSEEELLEEKKLEI 342
1BMD_A       294 VEGLEINeFARKRMEITAQELLDEMEQVK 322
gi 209586343 307 KTGLELSdLAMEKLQVTENELKEEREDAE 335
gi 5531799   303 VKLDLPK-DVLDEMKLSEAELMKEAADAL 330
gi 167537489 325 VKNIQLDeSQRDRIQASARELAAERHAAF 353
1CIV_A       335 VKDVIFDdYLSKKIKKSEDELLAEKKCVA 363
7MDH_B       325 ATDVSNDdFLWERIKKSEAELLAEKKCVA 353

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