1B26,1BVU,1L1F,1V9L


Conserved Protein Domain Family
NAD_bind_1_Glu_DH

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cd01076: NAD_bind_1_Glu_DH 
Click on image for an interactive view with Cn3D
NAD(P) binding domain of glutamate dehydrogenase, subgroup 1
Amino acid dehydrogenase (DH) is a widely distributed family of enzymes that catalyzes the oxidative deamination of an amino acid to its keto acid and ammonia with concomitant reduction of NADP+. Glutamate DH is a multidomain enzyme that catalyzes the reaction from glutamate to 2-oxyoglutarate and ammonia in the presence of NAD or NADP. It is present in all organisms. Enzymes involved in ammonia assimilation are typically NADP+-dependent, while those involved in glutamate catabolism are generally NAD+-dependent. Amino acid DH-like NAD(P)-binding domains are members of the Rossmann fold superfamily and include glutamate, leucine, and phenylalanine DHs, methylene tetrahydrofolate DH, methylene-tetrahydromethanopterin DH, methylene-tetrahydropholate DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha -beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.
Statistics
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PSSM-Id: 133445
View PSSM: cd01076
Aligned: 50 rows
Threshold Bit Score: 230.501
Threshold Setting Gi: 25028133
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       ###                    ##        
1B26_F       180 GGSKGREEATGRGVKVCAGLAMDVlg--------idPKKATVAVQGFGNVGQFAALLISQelGSKVVAVSDSrGGIYNPE 251
1V9L_F       180 WGNPVREYATGFGVAVATREMAKKlw--------ggIEGKTVAIQGMGNVGRWTAYWLEKm-GAKVIAVSDInGVAYRKE 250
gi 2494093   165 GGIAGRAESTGRGVQFAIQSSLRDtrtpgl-dgrrnLKGASTVIQGFGNVGYHAARFLSEedDARVTVLAERdGYVANPE 243
gi 66806963  217 GGIRGRTEATGLGVFYGIREFLSYeevlkktgltpgIKGKSIVIQGFGNVGYFAAKFFEQa-GAKVIAVAEHnGAVYNAD 295
gi 118534    209 GGIHGRISATGRGLFGHIENFIENasymsilgmtpgFGDKTFAVQGFGNVGLHSMRYLHRf-GAKCVAVGEFdGSIWNPD 287
gi 10281221  174 GGISGRTESTGLGVFYGCREILEDyefctqagipagLRGKNIIIQGYGAVGYYAAKYMCAy-GAKLVGVAEWdGSIYEEN 252
gi 17544676  241 GGIHGRVSATGRGVWKGLEVFTNDadymkmvgldtgLAGKTAIIQGFGNVGLHTHRYLHRa-GSKVIGIQEYdCAVYNPD 319
gi 39645909  247 GGIHGRISATGRGVFHGIENFINEasymsklgltpgFADKTFIIQGFGNVGLHSMRYLHRy-GAKCVGIAEIdGSIWNPN 325
gi 12644316  254 GGIHGRVSATGRGVFHGLENFINEanymsqigttpgWGGKTFIVQGFGNVGLHTTRYLTRa-GATCIGVIEHdGTLYNPE 332
gi 115663020 249 GGIHGRISATGRGVYHGVENFVNEasymsavsltpgLGDKSFIVQGFGNVGLHSTRYLHRy-GAKCVGVMEIdGSIYNPA 327
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                         ##                   ###       
1B26_F       252 GFDvEELIRYKKEHGtvvtypkg-------eritneeLLELDVDILVPAALEgAIHAGNAERIKAKAVVEGANGPTTPEA 324
1V9L_F       251 GLN-VELIQKNKGLTgpalvelfttkdnaefvknpdaIFKLDVDIFVPAAIEnVIRGDNAGLVKARLVVEGANGPTTPEA 329
gi 2494093   244 GLSiEALKQHQIRTGsilgfdga------ksiagdmcGVEQPCDVLIPAAMEnAIHAENAERMKAHLVVEAANGPVTFEA 317
gi 66806963  296 GLNiDALNKYKLQHGtfidfpga------tnivdsvkALEIPCDILIPAALEkQIHIGNVADIQAKLIGEAANGPMTPRA 369
gi 118534    288 GIDpKELEDYKLQHGtimgfpk--------aqklegsILETDCDILIPAASEkQLTKANAHKVKAKIIAEGANGPTTPQA 359
gi 10281221  253 GIDpDELQAFKEQRKgvkgfpka------seyhedesVIYKECDIFIPAAFEqTVNRANAPKFNCKVIAEAANGPTTLAA 326
gi 17544676  320 GIHpKELEDWKDANGtiknfpga------knfdpfteLMYEKCDIFVPAACEkSIHKENASRIQAKIIAEAANGPTTPAA 393
gi 39645909  326 GMDpKELEDYKLQHGtivgfpn--------sqpyegnILEAQCDILIPAAGEkQLTRKNAHNIKAKIIAEGANGPTTPDA 397
gi 12644316  333 GIDpKLLEDYKNEHGtivgyqna-------kpyegenLMFEKCDIFIPAAVEkVITSENANRIQAKIIAEAANGPTTPAA 405
gi 115663020 328 GIHpKELEDYKTEHGtivgfpg--------aepyegnLLTAQCDILVPCAGEkQITADNAHDIKAKIIAEGANGPTTPAA 399
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1B26_F       325 DEILSRRG-ILVVPDILANAGGVTVSYFEWvqdLQSFFWdld-------------------------------------- 365
1V9L_F       330 ERILYERG-VVVVPDILANAGGVIMSYLEWvenLQWYIWdee-------------------------------------- 370
gi 2494093   318 DEILRSRG-VTILPDLYVNAGGVVVSYFERvknLTHIPFglmerrrrergnhtiatalermt------------------ 378
gi 66806963  370 DQILLNRG-HVIIPDLLLNAGGVTVSYFEWlknLSHVRFgrlnkkweesskklllefvestv------------------ 430
gi 118534    360 DKIFLERN-IMVIPDLYLNAGGVTVSAFZZk-nLNHVSYgrltfkyerdsnyhllmsvqeslerkfg------------- 424
gi 10281221  327 EEILIKKG-VKFLPDILLNAGGVTVSYLQW---LQNLDHirpgrmtrrweetskyklleaiqist--------------- 387
gi 17544676  394 DRILLARGdCLIIPDMYVNSGGVTVSYFEWlknLNHVSYgrltfkydeeankmllasvqeslskav-------------- 459
gi 39645909  398 DKIFIERN-VMVIPDMYLNAGGVTVSYFEWlknLNHVSYgrltfkyerdsnyhllmsvqeslerkfg------------- 463
gi 12644316  406 DKILIDRN-ILVIPDLYINAGGVTVSFFEWlknLNHVSYgrltfkyeresnyhllasvqqsieriindesvqes-lerrf 483
gi 115663020 400 DAILLSKN-VLVIPDMFINAGGVTVSYFEWlknLNHVSYgrltfkyerdsnthllqsvqeslerkfgrhesvqeslerkf 478
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                              
1B26_F       366 --------------------------qvrNALEKMMKGAFNDVMKVkeky--nvdMRTAAYILAIDRVAY 407
1V9L_F       371 --------------------------etrKRLENIMVNNVERVYKRwqre-kgwtMRDAAIVTALERIYN 413
gi 2494093   379 ------gkespadmrdefleggaeidlvrSGLEDVMRSTWTRIADLmeqqpelgdYRTAAYVASIRQVAD 442
gi 66806963  431 ------nkklseaersliihgadeidivrSGLEDTMQNACAETRKTanek--ntdYRSAALYNAIMKIKA 492
gi 118534    425 -khggtipvvptaefqdrisgasekdivhSGLAYTMERSARQIMRTamkynlgldLRTAAYVNAIEKVFK 493
gi 10281221  388 ---glrvdvtknqqaakllegpsakdlvfTGLEESMAVAVQNTKETaskl--nisLRMAGYYNALMTIHQ 452
gi 17544676  460 ---gkdcpvepnaafaakiagasekdivhSGLEYTMQRSGEAIIRTahkynlgldIRTAAYANSIEKVYN 526
gi 39645909  464 -kqggpipivptadfqarvagasekdivhSGLAYTMERSARQIMRTankynlgldLRTAAYVNAIEKVFK 532
gi 12644316  484 grvggripvtpsesfqkrisgasekdivhSGLDYTMERSARAIMKTamkynlgldLRTAAYVNSIEKIFT 553
gi 115663020 479 grhgsgipitpsdqfkdriqgasekdivhSGLSYTMERSARQIMKTamkynlgldIRTAAYVNSIEKIFL 548

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