1LUA,1LU9


Conserved Protein Domain Family
NAD_bind_H4MPT_DH

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cd01078: NAD_bind_H4MPT_DH 
Click on image for an interactive view with Cn3D
NADP binding domain of methylene tetrahydromethanopterin dehydrogenase
Methylene Tetrahydromethanopterin Dehydrogenase (H4MPT DH) NADP binding domain. NADP-dependent H4MPT DH catalyzes the dehydrogenation of methylene- H4MPT and methylene-tetrahydrofolate (H4F) with NADP+ as cofactor. H4F and H4MPT are both cofactors that carry the one-carbon units between the formyl and methyl oxidation level. H4F and H4MPT are structurally analogous to each other with respect to the pterin moiety, but each has distinct side chain. H4MPT is present only in anaerobic methanogenic archaea and aerobic methylotrophic proteobacteria. H4MPT seems to have evolved independently from H4F and functions as a distinct carrier in C1 metabolism. Amino acid DH-like NAD(P)-binding domains are members of the Rossmann fold superfamily and include glutamate, leucine, and phenylalanine DHs, methylene tetrahydrofolate DH, methylene-tetrahydromethanopterin DH, methylene-tetrahydropholate DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha-beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.
Statistics
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PSSM-Id: 133446
View PSSM: cd01078
Aligned: 11 rows
Threshold Bit Score: 164.874
Threshold Setting Gi: 53802885
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
NADP binding
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:NADP binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #    #                                ####                    #    #           
1LUA_A        95 SNGSNTTAAAGVALVVKAAg------gSVKGKKAVVLAGTGPVGMRSAALLAGE-GAEVVLCGRKL-DKAQAAADSVn-k 165
1LU9_A        95 SNGSNTTAAAGVALVVKAAg------gSVKGKKAVVLAGTGPVGMRSAALLAGE-GAEVVLCGRKL-DKAQAAADSVn-k 165
gi 17368072   96 PAGSFTTGAAMVAEVNRALkar--fstDLKGKRIVIFGGAGVVAYVAAVIGALE-GAQTVLVGHDGeERVSKIAFTMk-w 171
gi 32476357  110 ASGCNTTAAAAVASASKHV--------SLNGCTAVVLGGTGPVGRRVAQMLARQ-NADVLLTSRSL-NRAEIACKEIgeh 179
gi 124268443  95 SNGSNTTAAAGVAWLAHGR--------SLAGKRAVVLAGTGPVGQRAAVMLAKE-GARVAITSRTL-ERAQAAARAIa-e 163
gi 53805251   95 SNGSNTTAAAGVAKLASSG--------TLAGKKAVVLAGTGPVGQRAAVMLAKEgCSEVVITSRQM-ARAEHACAEMk-a 164
gi 46277823  100 SNGSNTTAAAAVRAAGRHL--------DLSRANSLVLGGTGPVGQRVARLLARA-GGNVRLGSRQK-ERAEAVCAVIrah 169
gi 87306403   97 PNGANTTAAAAVLAAGRHL--------QLAGSETLVLAGTGPVGQRAARLLAQQ-GAHVRVASRRL-EKATKVCESLqae 166
gi 53802885   89 PSGAFTTAAAMVAMAEGELrkh--yqtGLEGKSVLALGGTGPVGQIAAVLAARA-GAQVKIIGRQL-EKAQKVATLCn-a 163
gi 20517381  124 TTGNSYTVATAIEGAIKAAelm---gkDVRDSHVVVIGATGSIGKVCAEILSRD-AKYMTLVARNK-EKLQNFRDYL--- 195
gi 46198495  336 TNGGAYTAGTVRAAIPKILahfaqsgkDLKGATAAVVGANGVVAFGIARQIAPL-VGRLILVGRDL-ERLKRAAESLr-- 411
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #### #                     #                       
1LUA_A       166 RFKVNVTAAETADDASRAEAVkgAHFVFTAGAIGLe--lLPQAAWQNEssIEIVADYNAQPPLGIGGIDATDKGkeyg-- 241
1LU9_A       166 RFKVNVTAAETADDASRAEAVkgAHFVFTAGAIGLe--lLPQAAWQNEssIEIVADYNAQPPLGIGGIDATDKGkeyg-- 241
gi 17368072  172 RFGIDVGAVDGTLPEARRAAItdADVILSAGPAGVsiltAEDLESAPK--LLVASDVNAVPPAGIAGIDVNAVDvplpt- 248
gi 32476357  180 VNSGNLEAAAPETPDDFRALLerADVIIASGAAGVel-aSKELIDSLSk-LRVAIDLNAVPPAGLGGVEAFDKAkllheg 257
gi 124268443 164 RFKIEVDAIEADDNAARARAIegANLVFATGAAGVa--lLDEAAWKDHpqLELLSDANATPTLGIEGIDMMDKGaqrh-- 239
gi 53805251  165 RFGVDLVPVEAFDDDARAAAIadAQIVFATGAAGVr--lLEECHWRDRpdLELLADANATPPLGLGGTDMMDRGtdrh-- 240
gi 46277823  170 VPGADIQAVSVASSSDAPTALdgRNLVVAAGAAGAvl-lPKKLRLACKs-LKVAIDLNGVPPAGIEGVELPDKAadrd-- 245
gi 87306403  167 VADGAFRPFAVSDDDSLKAALngVAVVIAAGAANVrlagQENLRQAAD--LQVLIDLNAAPPVGLEGVDPMDKAqdrd-- 242
gi 53802885  164 EFGEGQTAIEGDADANKGELLktADVVFATAAAGIqvlsAELLAASPR--LKVAADVNAVPPSGIAGLDPHHKGtpie-- 239
gi 20517381  196 MQKTGMSAHITSDVKEALEDA--DIIITVTSAVDT----VIKPEYLKP--GAVVCDVARPRDVSKEVADKRDDVlviegg 267
gi 46198495  412 KNLERKGEVPEILATTEIAAIreADLVFTATSDPN----PVIYPEHVKp-GAWIYDEGVPPDVHPSVREVPGVRvi---- 482
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                        #                                            
1LUA_A       242 ---gkraFGALGIGGLKLKLHRACIAKLFEs--sEGVFD-----------AEEIYKLAKEM 286
1LU9_A       242 ---gkraFGALGIGGLKLKLHRACIAKLFEs--sEGVFD-----------AEEIYKLAKEM 286
gi 17368072  249 --gkgvgIGALAVGNVKYQTQCRMFRKMLEaq-ePLCLD-----------FRDAYKLAVEI 295
gi 32476357  258 dedgpvaYGPIGVGGLKMRTHKAAIAKLFTs--nSLVLD-----------ADEIYDLTIEQ 305
gi 124268443 240 ---gktvFGAIGFGALKIALHRACIARMFEq--qDLVLD-----------GEEIYAIAKTM 284
gi 53805251  241 ---gkivWGAIGFGTLKLAVHRACIAKLFEr--nDQVFD-----------AEEIFALAKTM 285
gi 46277823  246 ---ghvcYGALGVGGTKMKVHRAAINKLFEs--nNAVLD-----------IEEVYSLALQL 290
gi 87306403  243 ---gricYGAIGVGGTKMKIHKRALARLFEq--nDLVLN-----------AEEVYAIGRTL 287
gi 53802885  240 ----gspSGAIGIGALAIGNVKYQAQHRLL----LRMRS-----------TDKPVYLAFNE 281
gi 20517381  268 vvevpgdVDFHFNFGFPPKTSYACMAETMIlameGRIENyslgrdltveqVDEIARLAKKH 328
gi 46198495  483 ------pGGVVRLPGEARATLDLHFGAPDQv---PACLAetm-----ilaAEEAFDRKSLG 529

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