1JBG


Conserved Protein Domain Family
HTH_TipAL-Mta

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cd01106: HTH_TipAL-Mta 
Click on image for an interactive view with Cn3D
Helix-Turn-Helix DNA binding domain of the transcription regulators TipAL, Mta, and SkgA
Helix-turn-helix (HTH) TipAL, Mta, and SkgA transcription regulators, and related proteins, N-terminal domain. TipAL regulates resistance to and activation by numerous cyclic thiopeptide antibiotics, such as thiostrepton. Mta is a global transcriptional regulator; the N-terminal DNA-binding domain of Mta interacts directly with the promoters of mta, bmr, blt, and ydfK, and induces transcription of these multidrug-efflux transport genes. SkgA has been shown to control stationary-phase expression of catalase-peroxidase in Caulobacter crescentus. These proteins are comprised of distinct domains that harbor an N-terminal active (DNA-binding) site and a regulatory (effector-binding) site. The conserved N-terminal domain of these transcription regulators contains winged HTH motifs that mediate DNA binding. These proteins share the N-terminal DNA binding domain with other transcription regulators of the MerR superfamily that promote transcription by reconfiguring the spacer between the -35 and -10 promoter elements. Unique to this family, is a TipAL-like, lineage specific Bacilli subgroup, which has five conserved cysteines in the C-terminus of the protein.
Statistics
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PSSM-Id: 133381
View PSSM: cd01106
Aligned: 79 rows
Threshold Bit Score: 85.2304
Threshold Setting Gi: 21205598
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
DNA bindingdimer interface
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:DNA binding residues [nucleic acid binding site]
Evidence:
  • Comment:Based on sequence similarity to BmrR and the structure of Bacillus subtilis BmrR bound to DNA (1EXI).

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###             #                ###                                           
1JBG_A        3 YQVKQVAEISGVSIRTLHHYDNIELLnPSALTDaGYRLYSdADLERLQQILFFKEIGFRLDEIKEMLDHpn------fDR 76
gi 52840853   4 YKIKEISKLTSLSIRTLQYYDEINLLkPSKRSQsDYRLYSsDDVLRLQQITTLRYLGCSLASIKEIIQHpd------fNL 77
gi 54294345   4 WFVKDLSQLTGVSVQTLHHYDRIGLLkPSLRLAnGYRVYSeKDLLKLQQIIALKFFGFELSQIKTLLAEe-------gNA 76
gi 55821576   8 FSTGELAKEAEVSIRTVQYYDQRGILtPSKVTEgGRRIYHeSDLERLKVICFLRDLDFSINQIKNLLQEenr----eqVL 83
gi 15675682   5 YSTGELANLAGVSIRTVQYYDQRGILiPTALTAgGRRLYTdSDLEQLRMICFLRDLGFSIEQIRKVLAEenaaqvlelLL 84
gi 25011832   5 YSTGDLAKEAGVTVRTVQYYDKRGILsPSELSEgGRRVYSiADLEKLRQIIYLRDLDFSIDNIKNLFTEdnasqilelFL 84
gi 42519877   5 YTIGEAAKKLQVSTRTLRFYEEKDLVrPAYTEEnGYRFYEkDQIRQLELILFLKELGFSLKQIKMLIQDehg----nqSV 80
gi 58338195   5 YTIGELAKKLNITTRTIRYYDQKGLVrPNSIGEnGYRFYTvEQVKQLKLVIFLKELGFSLKDIQKILEDeng----dkLI 80
gi 23100773   4 YSTGEVAKMLGVSVRSLRYYDQIGLIsPNKKDEyGKRFYSdDDIVTLKKITILKMSNLSLDNIKKILSEi--------TI 75
gi 89096596   4 WTTGEAAKQRNVSVRTLRYYDQIGLLaPSFKDEnGKRHYSeEDLFTLEKILILKSLSLPLEEIREVLKQl--------SY 75
                        90       100
                ....*....|....*....|....*....
Feature 1                                    
1JBG_A       77 KAALQSQKEILMKKKQRMDEMIQTIDRTL 105
gi 52840853  78 KTSLAVQAKALEKKAEQIHEASSLLNYIA 106
gi 54294345  77 LNHFNSQARVLEQKASTLLEGAKTLRRII 105
gi 55821576  84 ELLLTDQIESLEKSSKEIEVKLKRARHLQ 112
gi 15675682  85 VDHIATAKEDLAAKEQQVDIAVKILDRLR 113
gi 25011832  85 QVQIRELRLAIDSKKDKLDKAVNLLKTVE 113
gi 42519877  81 ELLLKEQYQENQRKIEEISKKQAQIKHLQ 109
gi 58338195  81 ELLLKDQLKRNKEETKKLEKQNKKIIQLQ 109
gi 23100773  76 EQLLYVHRDYLQQKIEELHASVHHTTALL 104
gi 89096596  76 KQILVSHYNHLQEQLSELQTSISNTASLI 104

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