1Q05


Conserved Protein Domain Family
HTH_CueR

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cd01108: HTH_CueR 
Click on image for an interactive view with Cn3D
Helix-Turn-Helix DNA binding domain of CueR-like transcription regulators
Helix-turn-helix (HTH) transcription regulators CueR and ActP, copper efflux regulators. In Bacillus subtilis, copper induced CueR regulates the copZA operon, preventing copper toxicity. In Rhizobium leguminosarum, ActP controls copper homeostasis; it detects cytoplasmic copper stress and activates transcription in response to increasing copper concentrations. These proteins are comprised of two distinct domains that harbor the regulatory (effector-binding) site and the active (DNA-binding) site. Their conserved N-terminal domains contain winged HTH motifs that mediate DNA binding, while the C-terminal domains have two conserved cysteines that define a monovalent copper ion binding site. These proteins share the N-terminal DNA binding domain with other transcription regulators of the MerR superfamily that promote transcription by reconfiguring the spacer between the -35 and -10 promoter elements.
Statistics
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PSSM-Id: 133383
View PSSM: cd01108
Aligned: 30 rows
Threshold Bit Score: 192.006
Threshold Setting Gi: 88940005
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:DNA binding residues [nucleic acid binding site]
Evidence:
  • Comment:Based on sequence similarity to BmrR and the structure of Bacillus subtilis BmrR bound to DNA (1EXI).

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###             #                ###                                           
1Q05_B        1 MNISDVAKITGLTSKAIRFYEEKGLVtpPMRSENGYRTYTQQHLNELTLLRQARQVGFNLEESGELVNLFndPQRHSADV 80
gi 75431633   1 MNITEVAQKTQLTQKSIRFYEEKGLItpPTRGVNGYRQYNASHIEELTLIHQARQVGFSLPVCKELLELYknPHRRSADV 80
gi 12056092  13 MNISQIAKLTSLTAKSIRLYEEKGLIipPLRSESGYRTYTQQHVDDLLLIARCRRVGFSLDECKAMLTLAndPNRTSAAV 92
gi 37200822   1 MNIGAIAKLTGLSSKSIRMYEEKGIIspPLRSDAGYREYGQKHIVELNLVSRAKNAGFSLQECKEFVQLAqnPNRKSSEV 80
gi 84393798   1 MNISEAAKLTNLSTKSIRLYEDKGVItaPFRSENGYRSYDKKQIKELGIVAKARSAGFSLDECRALVELAdnPCRESADV 80
gi 84500085   1 MNIGRAAKETGLTVKTIRYYEEIGLId-ADRARNGYRDFGDRQITRLRMLAQARHLGFGLEECRQLLDLDadPARASRDV 79
gi 24373255   1 MKIGEVAKHTGLSVKSIRYYHDIGLVc-AERNDAGYRVYRHRDIESLKFVHQCRDLGFSLEDCKLLLDLRnnDSRNAEDV 79
gi 1175213    1 MNISEAAKLVGLSTKQIRDYEKMGLIkpAVRSLSGYRNYGESDLERLHFIRHSRNVGFSLHQIAQLLALQdnPKRSCREV 80
gi 53728812   1 MNISQAAKHTELSAKQIRDYEKAGLLpqATRSSSGYRIYSAADLERLHFISNARKVGFSLVQISELLKLNddPHRTSREV 80
gi 32029731   1 MNIGQASKATGLSIKQIRDYEKVGLLsnTSRSASGYRIYGNDTLDRLKFIAKARGVGFSLAQIGELLALQdnPHRKSCEV 80
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*..
Feature 1                                                      
1Q05_B       81 KRRTLEKVAEIERHIEELQSMRDQLLALAnACPGDDSADCPIIENLS 127
gi 75431633  81 KARTMTRIAEIDTQIRQLQFMREKLMSLAqQCPGDDSENCPIINGLS 127
gi 12056092  93 RARAQEKWQEISRKLSELTMIKQQLEEWIaSCPGDQGSDCPIIEQLK 139
gi 37200822  81 KEKTKEKLAEIEQKLAHLHEIKKQLEGWVsSCPGNSDSHCPIIEELT 127
gi 84393798  81 KAKALSKLEEVNKKIEDLLVIQKTLKEWVeQCPGDSNSHCPIIDSLV 127
gi 84500085  80 KALALRNLAAVRDKIRQLQVLESQLETLIhECHGDDDPDCAILDSLS 126
gi 24373255  80 KQLTRNHLAYVELQISKLQNLRSQLQQMVsECQGGEQPHCTIINSLN 126
gi 1175213   81 KVLTAQHIATLNQQIEQLQKMVQKLQHWHdSCQGNDNPECLILNGLN 127
gi 53728812  81 KRLTEQHIEELQQKITDLQQMLTLLKSWSkSCCGNDSPECSILSGLK 127
gi 32029731  81 KALANMHIEFLTQKIEELTQMKATLQAWSdACTGDNAPDCPILKGLA 127

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