5MDH


Conserved Protein Domain Family
MDH_cytoplasmic_cytosolic

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cd01336: MDH_cytoplasmic_cytosolic 
Click on image for an interactive view with Cn3D
Cytoplasmic and cytosolic Malate dehydrogenases
MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. Members of this subfamily are eukaryotic MDHs localized to the cytoplasm and cytosol. MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133421
View PSSM: cd01336
Aligned: 28 rows
Threshold Bit Score: 524.112
Threshold Setting Gi: 78192396
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                # ####                         #                                        
5MDH_A         2 EPIRVLVTGAAGQIAYSLLYSIGNGSVFgKDQPIILVLLDITPmMGVLDGVLMELQDCALPLLKDVIATDKEEIAFKDLD 81
gi 168051601   4 DPVRVLVTGAAGQIGYALVPMIARGIMLgPDQPVILHMLDIPPaTESLNGVKMELTDAAFPLLKGVVATTDVAEACKDVN 83
gi 78192396    2 SAVKVAVTGAAGQIGYALVPLIARGILLgPTTHVELRLLDIEPaLKALAGVEAELDDCAFPLLDKVVITADPRVAFDGVS 81
gi 11133509    4 EPVRVLVTGAAGQIGYALVPMIARGIMLgADQPVILHMLDIPPaAEALNGVKMELIDAAFPLLKGVVATTDAVEGCTGVN 83
gi 15077032    4 TCKRVAVTGAAGQIGYSLLPLIAAGRMLgFDQRVQLQLLDISPaLKALEGIRAELMDCSFPLLDGVVITDEPKVAFDKAD 83
gi 116788082  52 EPARVLVTGAAGQIGYALVPMIARGIMLgPDQPVILHMLDIPPaAEALNGVKMELIDAAFPLLKGIVATTDPVEACSGVN 131
gi 146165401  44 DEINVCVTGAAGQIGYAFLPLLLTGQCF-GDKKINLRLLDVPQaESILQGVELELQDGAYPLLKSIKTGSNESILFQDVD 122
gi 24528077   10 RPINVCVTGAAGQIAYSLLPLIAGGKVFgPQQQVSLRLLEIEAaLPSLQGVVMELEDCAFPLLHSVFTTSDAREAFRSCD 89
gi 145497202  25 PPVRVTVTGAAGNIGYALVHMIGQGRLLgPNQQIILTLLELPMaKDQLEGTMMELRDCAFPILKEIRGTTQYDQGFMGCE 104
gi 118363871   5 AVINVCITGAAGNIAYALYSILCTGQIFgSNQKMNLKLLDIESqAKGLQGVALELEDCAYALVNSIEYGFDPEVLFKDMD 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            ####                 #                    # #                        #      
5MDH_A        82 VAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYAKkSVKVIVVGNPANTNCLTASKSAPs-iPKENFSCLTRLDHN 160
gi 168051601  84 IAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAkDVKVLVVANPANTNALILKEFAPs-iPEKNITCLTRLDHN 162
gi 78192396   82 ISIMCGAFPRRAGMERKDLLEMNARIFKEQGEAIAAVAApDCRVLVVGNPANTNALILLKSAQgklNPRHVTAMTRLDHN 161
gi 11133509   84 VAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAApNCKVLVVANPANTNALILKEFAPs-iPEKNISCLTRLDHN 162
gi 15077032   84 IAILCGAFPRKPGMERRDLLQTNAKIFSEQGRVLGEVASpNCRVCVVGNPANTNALILLRESKgklNPRFVTALTRLDHN 163
gi 116788082 132 IAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEQHAApNCKVLVVANPANTNALILKEFAPs-iPEKNITCLTRLDHN 210
gi 146165401 123 VAVFIGGFPRKPGMERKDLLTINGNIFKKQGQALDTVAKkTCKSLVVANPANTNCLILAETAKs-iPKQNFSALTRLDHN 201
gi 24528077   90 VAVLLGAFPRKQGMERKDLLEKNAGIFKEQGEALNTEAGaNVRVIVVGNPANTNAYILKHFAPn-iPDKNITALTRLDQN 168
gi 145497202 105 IAILVGAKPRGPGMERKDLLAANARIFKEQGEALEKYASkNVKVLVVGNPANTNALITAQFAPs-iPKSNFTALTRLDQN 183
gi 118363871  85 VGIFLGGQSRRPGMERVDLLQVNNRIFKIQGKALNKVAKpSTKILVIANPVNTNCLTLIQNCPn-iPKENFTCLTRLDHN 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 #                                                     #
5MDH_A       161 RAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVklqakEVGVYEAVKdDSWLKGEFITTVQQRGAAVIKARKLS 240
gi 168051601 163 RALGQVAERLNVPVSTVKNVIIWGNHSSSQYPDVNHAVIdspdgEKAVRDAVSdDAWLDGEFIKVVQQRGAAIIKARKLS 242
gi 78192396  162 RALSLLARKAGVPVSQVRNVIIWGNHSSTQVPDIDNAVIg----TTPAREAIK-DGALNDDFVQLVRERGAEIIQLRGLS 236
gi 11133509  163 RALGQISERLSVPVSDVKNVIIWGNHSSSQYPDVNHAKVqtssgEKPVRELVKdDAWLDGEFISTVQQRGAAIIKARKLS 242
gi 15077032  164 RATAQVAERARARVEEVKNCIIWGNHSGTQVPDVNSATVg----GKPARAAVDnDAFFDNEFITIVQERGAEIMKLRGLS 239
gi 116788082 211 RALGQISERLQVPVSDVKNVIIWGNHSSSQYPDVYHASVvtgagEKAVRQLVAdDAWLDGEFITTVQQRGAAIIKARKLS 290
gi 146165401 202 RAISQIALKAGCSITDVKNVIIWGNHSTTQYPDVNHGTVl----GKRIRQFINdEAYLNNAFIERVQKRGGEVLAARKNS 277
gi 24528077  169 RAVSLVARKLGVPLSKVSDVYVWGNHSSTQYPDVLHGSVe----GKGKLSYLLdKSYLEETFIPTIQKRGAAVIAARKMS 244
gi 145497202 184 RAQSIIAQRVSANVEDVRNIIIWGNHSTTQFADVSQATVqqngiSQTVRGLVAdDAWLQKAFVEQVAKRGGAIIEKRKAS 263
gi 118363871 164 RAIAQLAIKAKVPVTEVKNVILWGNHSLTMYPDHNHAHIe----GQPAVDIYE-EEWIKNTFIPKVQKRGGEVLNLRQNS 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
5MDH_A       241 SAMSAAKAICDHVRDIWFGTpeGEFVSMGIISDGNsYGVPDDLLYSFPVTIKDKT-WKIVEGLPINdFSREKMDLTAKEL 319
gi 168051601 243 SALSAASSACDHIRDWVLGTpkGSWVSMGVYSDGS-YNVPPGIIYSYPVTCANGD-WTIVQGLSIDeKSRAKLDATADEL 320
gi 78192396  237 SAMSAAKAAVDHVHDWIHGTpeGVYVSMGVYSDGNpYGVPSDLIFSFPCTCHAGE-WTVVSDKLNGdLGKQRLASTIAEL 315
gi 11133509  243 SALSAASSACDHIRDWVLGTpeGTFVSMGVYSDGS-YSVPSGLIYSFPVTCRNGD-WSIVQGLPIDeVSRKKMDLTAEEL 320
gi 15077032  240 SALSAAKAIVDHVHDWMLGTpsGTHVSMAVYSDGNpYGVPGGLIFSFPVTCSGGE-WQIVSGLNVTpAISERIKATTTEL 318
gi 116788082 291 SALSAASSACDHIRDWVLGTpkGTWVSMGVYSDGS-YDVPPGIIYSYPVTCENGS-WSIVQGLPINeFSRKKLDATANEL 368
gi 146165401 278 SVMSAANAVKDHLHDWYFGTksGEFVSMAVYSDGS-YGVPKGLIFSYPVTCSNFN-YKIVQGLNIDeFSKEKIRITTEEL 355
gi 24528077  245 SAMSAANAVGDHLHDWLLGS--DRIVSMSVASDGS-YGIEKGTIFSFPLRCSRGGtYEIVKGLQLDdFSQRYIKITADEL 321
gi 145497202 264 SAASAASAVCDHIHDWLIGTdnGTFVSMGVVTDGKlYGIKEQVCFSFPCICKDGN-VKVVEGLKWDqFQQSMIDKTLKEL 342
gi 118363871 239 SVMSAAIAIRDHLVNWIYGTpeGQFVSMGVYTDGSfYDIPADFVFSVPVKCKDFK-YEVVKDLTISqFSRQKINISLREL 317

                 ....*...
Feature 1                
5MDH_A       320 AEEKETAF 327
gi 168051601 321 VEEKALAY 328
gi 78192396  316 QEERAQAG 323
gi 11133509  321 KEEKDLAY 328
gi 15077032  319 EEERREVS 326
gi 116788082 369 VEEKALAY 376
gi 146165401 356 LSEKADAF 363
gi 24528077  322 YAEKAEAL 329
gi 145497202 343 LEEKEMAF 350
gi 118363871 318 QDEKEEAN 325

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