1IB6,1MLD,1SEV,2DFD,1SMK,1EMD


Conserved Protein Domain Family
MDH_glyoxysomal_mitochondrial

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cd01337: MDH_glyoxysomal_mitochondrial 
Click on image for an interactive view with Cn3D
Glyoxysomal and mitochondrial malate dehydrogenases
MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. Members of this subfamily are localized to the glycosome and mitochondria. MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133422
View PSSM: cd01337
Aligned: 57 rows
Threshold Bit Score: 401.097
Threshold Setting Gi: 45187709
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ####                     #                                              
1IB6_A         1 MKVAVLGAAGGIGQALALLLKTQLPSgSELSLYDIApVTPGVAVDLSHIPTAVKIKGFSGED------ATPALEGADVVL 74
gi 19075316   29 FKVAVLGAGGGIGQPLSMLLKLNDKV-SELALFDIR-GAPGVAADIGHINTTSNVVGYAPDDk----gLEKALNGADVVI 102
gi 547901     18 YKVTVLGAGGGIGQPLSLLLKLNHKV-TDLRLYDLK-GAKGVATDLSHIPTNSVVKGFTPEEpd---gLNNALKDTDMVL 92
gi 211581963   2 VKAVVLGAAGGIGQPLSLLLKACPLV-DDLALYDVV-NTPGVAADLSHISSVAKITGYLPKDd----gLKLALTGADVVV 75
gi 170096999   2 VKAVVLGAAGGIGQPLALLLKANPLI-TELGLYDIV-NTPGVAADLSHISTPAKVEGYLPADd----gLKKTLTGADIVV 75
gi 164655785   1 MKATVIGAAGGIGQPLSLLLKQSPYV-SDLSLYDVV-NAPGVAADLSHINTASPVQGFLPENd----gLAKAVQGSDLVI 74
gi 210075935   2 VKAVVAGAAGGIGQPLSLLLKLSPYV-TELALYDVV-NSPGVAADLSHISTKAKVTGYLPKDd----gLKNALTGANIVV 75
gi 45188125    2 ARVTVLGAAGGIGQPLSLLLKTCSLV-SELNLYDLR-NAPGVAADVSHVNTDCRVAGFEGPAe-----LGRALRGADLVV 74
gi 211590891   2 VKIALLGAAGQIGTPLSLLCKASDLF-DEISLYDIV-HVPGIATDLNHIDTRARVSGHLPDDs----gLKKALTGADIVV 75
gi 190345507   2 VKVTVCGAAGGIGQPLSLLLKLNPQV-SHLSLFDIV-NAHGVAADLSHICTPAVVTGHQPANkedssaITEALAGTDVVI 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # ##          #   #  #                   # #                          #  #     
1IB6_A        75 ISAGVARKPGMDRSDLFNVNAGIVKNLVQQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGvYDKNKLFGVTTLDIICS 154
gi 19075316  103 IPAGVPRKPGMTRDDLFATNASIVRDLAFAAGETCPEAKYLVVTNPVNSTVPIFKKALERVGvHQPKHLFGVTTLDSVRA 182
gi 547901     93 IPAGVPRKPGMTRDDLFAINASIVRDLAAATAESAPNAAILVISNPVNSTVPIVAQVLKNKGvYNPKKLFGVTTLDSIRA 172
gi 211581963  76 IPAGIPRKPGMTRDDLFKVNAGIVKGLVESIAEFAPKAFILVISNPVNSTVPIAAEVLKAAGvFDPKRLFGVTTLDVVRA 155
gi 170096999  76 IPAGVPRKPGMTRDDLFKINAGIVRDLATGIAATAPKAFVLVISNPVNSTVPIVAEVFKKAGvYDPKRLFGVTTLDVVRS 155
gi 164655785  75 IPAGMPRKPGQTRDDLFNANASIVYGIAEGIAKAAPKAFILVISNPVNSMVPIFAEVLKAHNvYDPKRLFGVTSLDLVRA 154
gi 210075935  76 IPAGIPRKPGMTRDDLFKINAGIVRDLVTGVAQYAPDAFVLIISNPVNSTVPIAAEVLKKHNvFNPKKLFGVTTLDVVRA 155
gi 45188125   75 IPAGVPRKPGMTRDDLFGINAGIVQSLVTAVAKHCPAARLLIISNPVNSTVPIAVETLKRLGkLNAARVFGVTTLDLVRA 154
gi 211590891  76 VTAGIARKPGMTRDDLFKTNAGIIRDIFTEIAATCPQAMCCVVTNPVNSTLPVATEALKKGGvFDPTRVFGVTTLDTVRA 155
gi 190345507  80 IPAGVPRKPGMTRADLFNINASIVRDLVANVGKTAPNAAILIISNPVNATVAIAAEVLKKLGvFNPRKLFGVTTLDSVRA 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   #                                                  # 
1IB6_A       155 NTFVAELKGKQPGEVE-----VPVIGGHSGVTILPLLSQVPGVSf-----tEQEVADLTKRIQNAGTEVVEAKAGGGSAT 224
gi 19075316  183 SRFTSQVTNGKAELLH-----IPVVGGHSGATIVPLLSQGGVELt------GEKRDALIHRIQFGGDEVVKAKAGAGSAT 251
gi 547901    173 ARFISEVENTDPTQER-----VNVIGGHSGITIIPLISQTNHKLm-----sDDKRHELIHRIQFGGDEVVKAKNGAGSAT 242
gi 211581963 156 ETFTQEFSGVKNAADAt----VPVIGGHSGETIVPLFSKVSPSFqi----pADRYDALVNRVQFGGDEVVKAKDGAGSAT 227
gi 170096999 156 STFVAEKIGDLSLSKDv---vVPVVGGHSGVTIVPLLSQSSHPLpas--vdTASLDALVNRIQFGGDEVVKAKGGAGSAT 230
gi 164655785 155 STFVSEAAGAKKDAANy---hVPVIGGHSGVTIVPLLSQAKPSFqa----dQQKIEELTNRIQFGGDEVVKAKNNAGSAT 227
gi 210075935 156 QTFTAAVVGESDPTKLn----IPVVGGHSGDTIVPLLSLTKPKVei----pADKLDDLVKRIQFGGDEVVQAKDGLGSAT 227
gi 45188125  155 QSFLADALGRDAHRVAdlashVTVVGGHAGRTIVPLLADAQLRElq----rRGLYDSYVHRVQFGGDEVVKAKAGAGSAT 230
gi 211590891 156 STFVAHALGDNANPKDf---kVPVIGGHSGATILPLYSQAQPPVsl----dGQTLAGVINRVQFGGDEIVKSKQGAGSAT 228
gi 190345507 160 ETFLGELTGQSPSAIRg---hLSVIGGHSGDTIVPLINVDSTISsavsqwsPSQYQAYVKRVQFGGDEVVKAKNGAGSAT 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #                                                                             
1IB6_A       225 LSMGQAAARFGLSLVRALQGe-------qGVVECAYVEGDG--------------QYARFFSQPLLLGKNGVEe--RKSI 281
gi 19075316  252 LSMAYAGARMASSVLRALAGe-------sGVEECTFVESPLyk-----------dQGIDFFASRVTLGKDGVDt--IHPV 311
gi 547901    243 LSMAHAGAKFANAVLSGFKGe-------rDVIEPSFVDSPLfk-----------sEGIEFFASPVTLGPDGIEk--IHPI 302
gi 211581963 228 LSMAFAGFRFAEAVIKASKGe-------kGIVEPTFVYLPGvaggd----eiakaTGLDFFSTPVELGVNGAEk-aINIL 295
gi 170096999 231 LSMAYAGAEFASKILRAVKGe-------eGIVAPSYVSLEAdpvgaaa-ltkelgAELAYFSSNIELGPQGVVk--INPL 300
gi 164655785 228 LSMAFAGARFANAVLAAAQGkk------aELPEFSYVDLAAdeaggka-vkdvigNDIAFFSVPLTLGPNGVEk--IQSL 298
gi 210075935 228 LSMAQAGFRFAEAVLKGAAGe-------kGIIEPAYIYLDGidgts----dikreVGVAFFSVPVEFGPEGAAk-aYNIL 295
gi 45188125  231 LSMALAGFHFAELLLRSLAGe-------aVAPEPAFVYLPGlpggadlaralaatEPVDYFAAPVRLAAGDAAvvdTAFV 303
gi 211590891 229 TCMAYAGFRFIKALLVGMDGe--------AITEEAYVYLPGipggk----eiaaeLGVDYFAVKIELGKTGATk--ALPI 294
gi 190345507 237 LSMAYAGYRFAETVLNSLSDpskpvadstPVPDSSYVYLPGlpggee--fskkyvNGVTFFSVPVLLQNGEISs-fINPF 313
                        330       340       350
                 ....*....|....*....|....*....|.
Feature 1                                       
1IB6_A       282 GTLSA--FEQNALEGMLDTLKKDIALGQEFV 310
gi 19075316  312 GKIND--YEESLLKVALGELKKSITKGEQFV 340
gi 547901    303 GELSS--EEEEMLQKCKETLKKNIEKGVNFV 331
gi 211581963 296 EGVTE--QEKKLLEACIKGLQGNIEKGVEFA 324
gi 170096999 301 GKITE--AEQTLIKAAIPELQKNITTGVTFI 329
gi 164655785 299 GDISS--FESELIKKSIESLKGNIEKGVSFK 327
gi 210075935 296 PEAND--YEKKLLKVSIDGLYGNIAKGEEFI 324
gi 45188125  304 ADLAP--EQRALVDAALPELAASIRKGVDFV 332
gi 211590891 295 GKISE--HETRLLEVALKELKTNIATGLSFM 323
gi 190345507 314 EKYKItsEEANLTEVALDGLQKSIDQGTTFV 344

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