1CIV,1BMD,7MDH,1B8V,3D5T,1WZE


Conserved Protein Domain Family
MDH_choloroplast_like

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cd01338: MDH_choloroplast_like 
Click on image for an interactive view with Cn3D
Chloroplast-like malate dehydrogenases
MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. Members of this subfamily are bacterial MDHs, and plant MDHs localized to the choloroplasts. MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133423
View PSSM: cd01338
Aligned: 37 rows
Threshold Bit Score: 493.643
Threshold Setting Gi: 8979401
Created: 30-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #  ###                         ##                                       
1CIV_A        41 KIINVAVSGAAGMISNHLLFKLASGEVFGpDQPISLKLLGSERS-----FAALEGVAMELEDSLYPLLRQVSIGID--PY 113
gi 48428246    3 KHVKVAVTGAAGQIGYALLFRLASGQAFGlDTTVDLHLLEIEPA-----LPALKGVVMELEDCAFPLLRNMVVTSD--PR 75
gi 59799810    4 NRVRVAVTGAAGQIGYALVFRIASGQMFGpNTEVELNLLELEPA-----LPSLEGVAMELDDCAFPLLKRIVCTAD--LN 76
gi 48428252    3 KTVKVAVTGAAGQIGYSLLFRIASGQMFGaDTAVEIQMLELEAA-----IPAAKGVIMELEDCAFPLLQKVTVSSD--LD 75
gi 152032582   3 DPIRVAVTGAAGQIAYSLLVRLASGQLFGkDRKVELKLLEIPQA-----MGPLEGVMMELQDCAFPTLAKVEAFDN--PE 75
gi 50400730    3 QPIKIAISGGAGQIAYSLLFRLASGELFGpNQLIELQVLEVPNA-----LSALEGVKMEIEDCAFPLLSSIKICSD--PY 75
gi 149197839   3 KTIRVCVTGAAGQIDYSLLFRIASGEMFGpEQKVALNLLEITPA-----LDALKGVAMELDDCAFPLLEEIVMTDD--VN 75
gi 160871967   5 KPIKIAITGAAGQIGYALLFRIASGQMFGaDQPVILHLLELEKS-----LPMLNGVAMELEDCAFPLLKNIICTSE--LK 77
gi 149175155   3 HPIRVAVTGAAGQIGYAMLFRLASGEIFGpDQPVILHLVEIPPM-----LSALDGVEMELDDCAFPTLAGVVKADSdhLE 77
gi 168701574   3 KVVRVAVTGAAGRVSSSLIVRLASGEVFGpETKVVLQLLEVPASvnpaaLKNLEGAMYELQDCGFSTLKDVVITDD--PN 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     #                                       # #                       #
1CIV_A       114 EIFQDAEWALLIGAKPRGPGMERADLLDINGQIFAEQGKALNAVASpNVKVMVVGNPCNTNALICLKNAPnIPPKNFHAL 193
gi 48428246   76 VAFNDVNWALLVGAAPRKAGMERKDLLEKNGSIFAGQGKAINENAAsDVRIFVVGNPCNTNCLIAMNNAPdIPKDRFYAM 155
gi 59799810   77 KAMDGVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASdDVRVFVVGNPCNTNCLIAMNHAKdVPSDRFYAM 156
gi 48428252   76 TAFKEINWALLVGSVPRKAGMERGDLLKINGGIFVNQGKAIEKNAAsDVRILVVGNPCNTNCLIAMNNAKgISQDRWFAM 155
gi 152032582  76 QAFDGINWCLMVGSRPRGPGMERSDLIKINGPIFVNQGKALNRAAQ-DVRAVVVGNPCNTNCMIAAHNSD-VPHERFSAM 153
gi 50400730   76 QAFEDIDYALLIGAKSRGPGMERRDLLQENSKIFVNQGQALNAVAKsSAKIFVVGNPCNTNCLIALNNAPsLKRENFYAM 155
gi 149197839  76 VAMKDVNWALLVGSKPRGPGMERGDLIKENGPIFTSTGKAINDHAAdDVRVVVVGNPCNTNCLIAMHNAPdVPRDRFHAM 155
gi 160871967  78 ESMKDINWAILVGSMPRKAGMERADLLKINGCIFAPQGKAMNAYAAsDVKIFVVGNPCNTNCLITMHHAPdIPKSRFFAM 157
gi 149175155  78 DAFADCNFVVCVGSIPRKAGMERGDLIRINGPIFTSTGKAIEAAAAeDVRVLVVGNPCNTNCLIAMSNAPkVPRDRWYAM 157
gi 168701574  81 VAFNGCNYALLVGAAPRGPGEQRSDLIRKNGAIFVGQGQAIAKNAAsDVRILVVGNPCNTNCLIAYSNGRdVPADRWHAM 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #                                                
1CIV_A       194 TRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPVtevIRDRKWLEDEFTNMVQTRGGVLIKKWG 273
gi 48428246  156 TRLDQNRAIGQLALKAGVDVPSVKNMIIWGNHSSTQYPDFYHATIDGKPAtevIRDKNWLLNDFISVIQQRGAAVIKARG 235
gi 59799810  157 TTLDELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAaqvINDETWLKETFVSTVQQRGAAVIKARG 236
gi 48428252  156 TKLDENRAKSQLASKAGVPVKEVTHLGIWGNHSATQYPDFYNTKISGKPVtdvISDHEWLKGDFIKNVQQRGAEIIKARG 235
gi 152032582 154 MRLDQNRAKYLLASKAGAQVIDVTNVVIWGNHSNNQVPDFEFAKIGGKPVpevIADAAWLENAFMPTVQNRGAAVIKARG 233
gi 50400730  156 TRLDQNRATFFLSQKSQVSTKDVSCVTIWGNHSATQVPDFVNAKISQKPVetiIPDRQWLEKDFIESVQKRGAAIIQARG 235
gi 149197839 156 TRLDENRAKSQLAQKAGVAVTEVKNMVIWGNHSATQVPDYKNATISGKPAadvIGDLAYLQSEFTSTVAKRGAAIIAARG 235
gi 160871967 158 TLLDENRARAQLAKKAAVSVSEVTQMAVWGNHSSTQYPDFYHAKIAHQAVidvIPDKNWLENDFIHRVQKRGAAVIKARG 237
gi 149175155 158 TRLDQNRAMTQIAKKAGQPVSAVKNMNIWGNHSATQFPDFYHATIHGNPVpeiIEDHDWLRGEFIETVQKRGAAVIQARG 237
gi 168701574 161 TRLDHNRAVSALAIKAGVTNDAVTCVTIWGNHSNTQYPDFTNAKIGGRPAtevITDRAWLENTFVPNTQERGKFIIDTTK 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1CIV_A       274 RSSAASTAVSIVDAIRSLVTPTpegDWFSTGVYTNGnPYGIAEDIVFSMPCRSKGDGdyEFVKDVIFDDYLSKKIKKSED 353
gi 48428246  236 ASSAASAANAALDSVWSLINTTpadDNYSVALCAQG-QYGVDEGLIFSFPCRTENGVv-SVIEEIEHNEFGQQKLKETLD 313
gi 59799810  237 SSSAASAANAIITGVNHLVTDTpagESFSMCRRSKG-EYGVDEGLIFSFPCRREHGEl-KVVENLEFNDFGRERFNTTLN 314
gi 48428252  236 ASSAASAANGVVDTVRGIITPTapgDCFSAAVVSDG-SYGAEKGLIFGFPLKSDGKKv-EIIQGISLNDFAKEKFKITHE 313
gi 152032582 234 ASSAASAANAALDHVRSLITPTpagDTFCAAVMANG-AYGVDAGLIAGMPLTSTGHGdwSIVEGVPMSPFIKGKFDAVLD 312
gi 50400730  236 KSSAASAASALLDAMRDRILPTptgQWFSTALLSDGnPYGIEEGLIFSFPCRVKKNGelSIVSGLKWDAFLEEKIKLTEQ 315
gi 149197839 236 KSSAASAANGLIDHVKSLLTPTpegEVFSSCVCSDGnPYGIPEGLIFSFPCRSNGDGtyEIVPGFELDSHLTDGVAKTVA 315
gi 160871967 238 ASSAASAANAVVDSIYHLTHDTqkdDFFSVASCSMG-QYGVDEGLIFSFPSRVENNQl-KVVTDIQHNLFGEEKIKLSLN 315
gi 149175155 238 ASSAASAANAALDTIKSIITPTplgESFSAAVCSDG-SYGVDEGLICGYPLTSDGTTw-KIVEGQEHDDFAQAKFEATLQ 315
gi 168701574 241 VSSSFSAANGAIDHVKSLVTGTpagDWTSAAIVSKG-EYGVPAGLVFGYPCTTSGDGnwNVVEGLKLDAFGEAKFKKTLD 319
                        330
                 ....*....|.
Feature 1                   
1CIV_A       354 ELLAEKKCVAH 364
gi 48428246  314 ELREERDAVEA 324
gi 59799810  315 ELRSERDTVKS 325
gi 48428252  314 ELVSERNEVKE 324
gi 152032582 313 ELRREREMVKD 323
gi 50400730  316 ELKEEREMVSS 326
gi 149197839 316 ELEGEREVIKG 326
gi 160871967 316 ELRAEKNEVKE 326
gi 149175155 316 ELRDERDVVRD 326
gi 168701574 320 ELLKEQEVVKD 330

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