Conserved Protein Domain Family
AAT_I

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cd01494: AAT_I 
Click on image for an interactive view with Cn3D
Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the substrate in four kinds of reactions (1) transamination (movement of amino groups), (2) racemization (redistribution of enantiomers), (3) decarboxylation (removing COOH groups), and (4) various side-chain reactions depending on the enzyme involved. Pyridoxal phosphate (PLP) dependent enzymes were previously classified into alpha, beta and gamma classes, based on the chemical characteristics (carbon atom involved) of the reaction they catalyzed. The availability of several structures allowed a comprehensive analysis of the evolutionary classification of PLP dependent enzymes, and it was found that the functional classification did not always agree with the evolutionary history of these enzymes. Structure and sequence analysis has revealed that the PLP dependent enzymes can be classified into four major groups of different evolutionary origin: aspartate aminotransferase superfamily (fold type I), tryptophan synthase beta superfamily (fold type II), alanine racemase superfamily (fold type III), and D-amino acid superfamily (fold type IV) and Glycogen phophorylase family (fold type V).
Statistics
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PSSM-Id: 99742
View PSSM: cd01494
Aligned: 20 rows
Threshold Bit Score: 88.9787
Threshold Setting Gi: 27066022
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
catalyticpyridoxal
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Comment:the catalytic lysine in most cases is part of a flexible region
  • Structure:2EO5_A; 4-aminobutyrate-aminotransferase, catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D
  • Structure:1BJ4_A; serine hydroxymethyltransferase, catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D
  • Citation:PMID 7748903

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2EO5_A        87 PQLELAKKLvtysp--------gnfqkKVFFSn-sGTEAIEASIKVVknt------------------grkYIIAFlggf 139
1H0C_A        56 IMDEIKEGIqyvfq---------trnpLTLVIsgsGHCALEAALVNVlep-------------------gdSFLVGangi 107
1WYU_B       108 LMWELGEYLkalt-----------gmdAITLEpaaGAHGELTGILIIrayhedr----------gegrtrrVVLVPdsah 166
1BJ4_A        82 LETLCQKRAlqaykl-------dpqcwGVNVQpysGSPANFAVYTALvep-------------------hgRIMGLdlpd 135
1JS6_A       114 LETVMMDWLgkmlqlpeaflageagegGGVIQg-sASEATLVALLAArtkvvrrlqaaspgltqgavleklVAYASdqah 192
2TPL_B        76 NFYHLERTVqelf-----------gfkHIVPTh-qGRGAENLLSQLAikp-------------------gqYVAGNmyft 124
gi 159184934  70 AYEAAREKVrrfln--------agsvdEIVFTk-sSTEAINTVAYGYgmpvi---------------gegdEIVISimeh 125
2E7J_A        54 PIHDFIHNQlpkf----------lgcdVARVTn-gAREAKFAVMHSLakk-------------------daWVVMDench 103
1N8P_A        56 NRENLERAVaale-----------naqYGLAFs-sGSATTATILQSLpq--------------------gsHAVSIgdvy 103
1AJS_A        75 GLAEFRTCAsrlalgdds-palqekrvGGVQSl-gGTGALRIGAEFLarwyng------------tnnkdtPVYVSsptw 140
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2EO5_A       140 hgrtfgsisltaskavqrsivgpfmpGVIHVpypnpyrnpwhingyenpselvnrviefiedyifvnlvppeeVAGIFFE 219
1H0C_A       108 wgqra------------vdigerigaRVHPMtkdpgghytlq---------------------eveeglaqhkPVLLFLT 154
1WYU_B       167 gsnp--------------atasmagyQVREIpsgpegevdl----------------------ealkrelgphVAALMLT 210
1BJ4_A       136 gghlthgfm----tdkkkisatsiffESMPYkvnpdtgyiny--------------------dqleenarlfhPKLIIAG 191
1JS6_A       193 ssve--------------ragliggvKLKAIpsdgkfamrasal----------------qealerdkaagliPFFVVAT 242
2TPL_B       125 ttry---------------hqekngaVFVDIvrdeahdaglniafkgd--------idlkklqklidekgaenIAYICLA 181
gi 159184934 126 hsnivp----------whfirerqgaKLVWVpvdddgafhie---------------------afeksltertKLVAITH 174
2E7J_A       104 yssy--------------vaaeraglNIALVpktdypdyaitpen---------------faqtieetkkrgeVVLALIT 154
1N8P_A       104 ggthry----------ftkvanahgvETSFTndllndl----------------------------pqlikenTKLVWIE 145
1AJS_A       141 enhng-------------vfttagfkDIRSYrywdtekrgldl------------------qgflsdlenapeFSIFVLH 189
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                   #    
2EO5_A       220 piqgeggyvippknffaelqklakkygILLVDDEVQmglgrtgklf------------aienfntvpDVITLA--KAlgg 285
1H0C_A       155 hgesstgvlqpl----dgfgelchrykCLLLVDSVAslggtpl-----------------ymdrqgiDILYSGsqKAlna 213
1WYU_B       211 npntlglferri----leisrlckeagVQLYYDGANlnaimgwa----------------rpgdmgfDVVHLNlhKTftv 270
1BJ4_A       192 tscysrnleya------rlrkiadengAYLMADMAHisglvaagv--------------vpspfehcHVVTTTthKTlrg 251
1JS6_A       243 lgttsccsfdnl----levgpicheedIWLHVDAAYagsaficpef-----------rhllngvefaDSFNFNphKWllv 307
2TPL_B       182 vtvnlaggqpvsmanmravreltaahgIKVFYDATRcvenayfikeqeqgfenksiaeivhemfsyaDGCTMSgkXDclv 261
gi 159184934 175 msnalgtilpv-----keicriahergIPVLVDGSQgavhmpv-----------------dvrdidcDWYVMTghKLygp 232
2E7J_A       155 ypdgnygnlpdv----kkiakvcseydVPLLVNGAYaigrmpv-----------------slkeigaDFIVGSghKSmaa 213
1N8P_A       146 tptnptlkvtdiqkvadlikkhaagqdVILVVDNTFlspyisn------------------plnfgaDIVVHSatKYing 207
1AJS_A       190 acahnptgtdptpeqwkqiasvmkrrfLFPFFDSAYqgfasgnlekda-------wairyfvsegfeLFCAQSfsKNfgl 262
                        250
                 ....*....|....*
Feature 1                       
2EO5_A       286 g----impIGATIFR 296
1H0C_A       214 -----ppgTSLISFS 223
1WYU_B       271 phggggpgSGPVGVK 285
1BJ4_A       252 ------crAGMIFYR 260
1JS6_A       308 -----nfdCSAMWVK 317
2TPL_B       262 ------niGGFLCMN 270
gi 159184934 233 ------sgIGVLYGK 241
2E7J_A       214 -----sgpIGVMGMK 223
1N8P_A       208 h---sdvvLGVLATN 219
1AJS_A       263 y----nerVGNLTVV 273

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