Conserved Protein Domain Family
PBP1_SalR

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cd01545: PBP1_SalR 
Ligand-binding domain of DNA transcription repressor SalR, a member of the LacI-GalR family of bacterial transcription regulators
Ligand-binding domain of DNA transcription repressor SalR, a member of the LacI-GalR family of bacterial transcription regulators. The SalR binds to glucose based compound Salicin which is chemically related to aspirin. The ligand-binding of SalR is structurally homologous to the periplasmic sugar-binding domain of ABC-transporters and both domains contain the type I periplasmic binding protein-like fold. The LacI-GalR family repressors are composed of two functional domains: an N-terminal HTH (helix-turn-helix) domain, which is responsible for the DNA-binding specificity, and a C-terminal ligand-binding domain, which is homologous to the type I periplasmic binding proteins. As also observed in the periplasmic binding proteins, the C-terminal domain of the bacterial transcription repressor undergoes a conformational change upon ligand binding which in turn changes the DNA binding affinity of the repressor.
Statistics
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PSSM-Id: 107258
View PSSM: cd01545
Aligned: 35 rows
Threshold Bit Score: 309.871
Threshold Setting Gi: 16126594
Created: 1-Jul-2004
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative ligandputative
Feature 1:putative ligand binding site [chemical binding site]
Evidence:
  • Comment:based on sequence similarity to other members of the LacI family.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    ##  #                                             #                 
gi 103486410  77 TIADWTARQGTDWVDQMLLGGMLKCaeyGYRLIFELVDthsdhvereLRATIAALQPDGVILTPPHSDnplivrlLERQR 156
gi 76875219   63 IIGCIYDNPSKSYITRVQTGALAACqenNYNLLIHPCElrgealinnIDQLLQSSRLDGIVLTPPFSDfaelvdfLKSKK 142
gi 71146481   63 IITLLYSNPNPGYILELQNGALSQCieqGYNLQIHPCDhnspdliksVESITQYSTQDGFILTHPLCDnkelialLDEKN 142
gi 90022684   61 SIGFIYDNPNSNYVIDMQRGILNEChkrGYELVIHPCNasgehiideVIEMIDRSRVGGLVLTPPISEnpeilaaIANKK 140
gi 146275739  68 LIGLIHDNPNAQMVLGVQEGILSAIrdtEFALVVRPVNrrspdmlddVRAFLEQQRLFGVMLLPPISEndaladlCEDLG 147
gi 103488509  72 LIALLHDNPNAQTVLNFQRGVLDAIkdsDLALLVRPVDrgsdkllddVRTFLEKQRPIGAMLLPPISEndelaalCEDLG 151
gi 114797136  72 LVGLVYDSPSPQYVVNMQRGILDALedtDFQLVLRPVSrtdanypgrLRQFIQHHKPFGLILPPSVSEdeelsqmFRDHD 151
gi 28198590   65 VIGLVYDNPNPYHIINIQNGVLAACretGFSLQIYPCDstspllveeLTEWVQRSRQAGLVLTAPMSErpeliagLIARG 144
gi 71145219   63 ILSLLYSNPNPGYILEVQNGIQAQCdldNYNLQILPCDhndehffekIESLLQRSSTDGVLLIEPLCDmpevidlLDKYK 142
gi 24375020   62 AIGFIYDNPNAYYIIDMQNGILSACkdkGYELVIHPCNaksdticdeLTQLVKYSRLAGLVLTPPLSEdpkvlksLNEID 141
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #              #                                   #                      
gi 103486410 157 IPFARIGsrgg---gagiALVMDDESMARHATRHLIDLGHRRIAFIAGsseyplSQWRVDGWESEMRAAGlpt-agLVAR 232
gi 76875219  143 IPYARVAsavle--ddsiSVRSNDEQGAFEITEHLITLGHKSIAFIKGhpdhsaTEQRFKGYRRALASHGiefeerLVEE 220
gi 71146481  143 IPFARISpfvre--hdspYVMSNDEEASFEMTKYLISLGHTKIGFIKGhpdfgaTHKRYKGYQRALENMGltldedYVKQ 220
gi 90022684  141 VEFVRILsgsaapdtlspCVYIDDRTAAYTITQHLIDLNHKDIAFLGGdeehksSGERLEGYRSALADNGitphenHILP 220
gi 146275739 148 VTYVRMGsvrld--ddehMVASNDREAVARAVAYLVDHGHRRIGFVAGpdgfrsAAERASGYVDALETAGikqdatIMAE 225
gi 103488509 152 VRYVRIGsapld--dakhCISSNDREVVAAAVRGLIALGHRRIGFVRGpagfrsAAEREKGFLEALAEAGltlppeLNAP 229
gi 114797136 152 VDYVRIAsgmld--aparMILTHDGEGAAQAARHLCALGHTDIAHIHGpesfrcAHERRSGFQRGLAEAGvtlhpeLTVE 229
gi 28198590  145 IKFVRIIaated-pcdgpCVYIADRKAAYEITEHLIQLGHQRIGFLWGgaqhrsSGERYAGYEAALKDYGidldkrLVVP 223
gi 71145219  143 LPYALISpatsk--pkapFARSNDEQASYQMTEYLISQGHKDIAFIMGdpshgdSHQRLKGYQNALAEYNisvddtFFEQ 220
gi 24375020  142 ANYVRIIagegiiepdglTILVNDKSGAVEITQHLIDLGHKNIAFLSGdlhhesTKERLLGYKQALTKNHlilnenYIIE 221
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #                                                      #                #     
gi 103486410 233 GDFTYESGAAATRQLLGhp-drPSAIIASNDQMALAALEVARELGIeiPSQLSLVSFDNTPivrftQPTLTAVDQPVAET 311
gi 76875219  221 GNFSYHSGVDSARSILDls-prPTAVFASNDYMAAAVLKLATQRSLrvPDDISIAGFDNAPiarhiWPGLTTIAQPVEEM 299
gi 71146481  221 GMFTFETGESCARQLLNqe-srPTAIFASNDYMAAAILKVAAQMKIniPADLSVVGYDDAPvsqqiWPSITTVKQPINQM 299
gi 90022684  221 GKYSFESGVERTRALLEldgprPTAVFACNDEIAAGTLFAARIAGVdvPNQLSIVGFEDSPfsrqaWPNLTTAQQPTSTI 300
gi 146275739 226 GNYTFETGIAAGEKLLAas-prPTAIFASNDEMAAGVLHAARKKGLsvPEDLSIVGFDDTAiaahiWPPLTTVRWPIQAM 304
gi 103488509 230 GNYRYAAGIEAGEALLAra-dpPTAIFCSNDEMAAGVLSVAHGKGIkvPAELSIIGFDDSPtathiWPALSTVRWPIREM 308
gi 114797136 230 AGYTFDSGLLAMERLLYgk-rrPTAVFAGNDEMATGVYVAVRKAGLriPEDVSIVGFDDTPiagrlWPALTTVRLPIREM 308
gi 28198590  224 GDYTFDDGFRGARRLLAlr-dqPTEIFGSNDEIAAGVLAAARSVGInvPHHLSITGFENSPfashsSPTLTTASQTTDEI 302
gi 71145219  221 GYFTFESGEISARRLLSlk-nrPSAIFASNDYMAAAVLKVAAQKQIkvPQELSIVGYDDTPvskqiWPALTTVRQPIRDI 299
gi 24375020  222 GKYSFESGVEGANTLIKlk-nrPTAIVACNDEIAAGALFAARLAGLdiPGDLSIVGFEDSPfsrqtWPKLTTVHQPNSEI 300
                        250       260       270
                 ....*....|....*....|....*....|....*...
Feature 1                                              
gi 103486410 312 VSRAVEMIIkaqrg-----eklppQPVIVAGGFVERES 344
gi 76875219  300 TKQAVTQLIahiiq-----pqevaYQVTLEARLITRES 332
gi 71146481  300 ANTVVKKLIkrikk-----qpfeeFDTVFDCELIIRNS 332
gi 90022684  301 AQRATALLIdtlksra--egsqvvESEGFLPKLIVRDS 336
gi 146275739 305 AKAAAIKLIdpae--------arrQPSHFLSDLIERAS 334
gi 103488509 309 GARAAQILVpdflgp---gakvddEDNVLPSTLVERQS 343
gi 114797136 309 GHAAARLLLdqaag------iereEVTSFTPEIIVRES 340
gi 28198590  303 ARHAARLLIgqlrmdtcdeqsmllQNRGFVPQLVVRAS 340
gi 71145219  300 SSHATKKLIaqlnk-----csfdeVKTLFECQLVVRES 332
gi 24375020  301 AQVATELLIakrre------qdakFTKTFTPEPVIRDS 332

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