Conserved Protein Domain Family
Thr-synth_1

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cd01563: Thr-synth_1 
Click on image for an interactive view with Cn3D
Threonine synthase is a pyridoxal phosphate (PLP) dependent enzyme that catalyses the last reaction in the synthesis of threonine from aspartate. It proceeds by converting O-phospho-L-homoserine (OPH) into threonine and inorganic phosphate. In plants, OPH is an intermediate between the methionine and threonine/isoleucine pathways. Thus threonine synthase competes for OPH with cystathionine-gamma-synthase, the first enzyme in the methionine pathway. These enzymes are in general dimers. Members of this CD, Thr-synth_1, are widely distributed in bacteria, archaea and higher plants.
Statistics
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PSSM-Id: 107206
View PSSM: cd01563
Aligned: 39 rows
Threshold Bit Score: 302.59
Threshold Setting Gi: 29829733
Created: 1-Aug-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:2D1F_A; catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                               #                       
1E5X_A      108 WSKKEWVLPeid-ddDIVSAFEGNSNLFWAerfgkqflgxnDLWVKHCGISHTGSFKDLGXTVLVSQVNRLrkxkrpvVG 186
2D1F_A       16 IAAYRDRLPvgd-dwTPVTLLEGGTPLIAAtnlsk--qtgcTIHLKVEGLNPTGSFKDRGMTMAVTDALAHg-----qRA 87
gi 13488402  61 LWRFAHMLPct--eaEAVSLGEGLTPLLAApriga-flgvpNLAIKDEGRNPTWSHKDRFSTIAVSVARARg-----aKI 132
gi 15789760  59 LWRYAELLPft--rgSAVSMDEGATALVPCpglad-elgvgELYIKDEGRNPTGTFRDRGQTVAMTAAAQHg-----aSE 130
gi 15790946  49 LWAFSEFLPva--adDAVSLGEGWTPLVSAse--------wDAEFKLDYVCPSGSYKDRGAAVLMTRAAALg-----vDR 113
gi 18977552  43 WERFSEFFPle--ldPELSLGEGDTPLTRAkklsk--elgiELYLKNETINPTWSFKDRGTYLGIHRAIQLd-----fRR 113
gi 27379888 125 MWRYRELLPvrk-ckDIVSLGEVTTPLIRLpklgk-klgggEIIVKDEGRLPTGSFKARGLVMAVSMGKALg-----iKH 197
gi 28210306  52 LERYQDFFPfde-inNELSLNEGFTSLVSSprmak-rigikNIYFKNESQNPTWSFKDRGTLFGIHHAYSMg-----yKK 124
gi 29829733  53 LWRYAECLPla---aPTTSLGEGRTPLVSLt---------dGVAAKLDFLMPTLSFKDRGAVLLAELALQLr-----pRQ 115
gi 32035415  48 LFRYRKFIAlqdeswRDISLGEGMTPIVRFd---------eNVLLKMDYFMPTLSFKDRGAAVLISHCKSIg-----vDS 113
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1E5X_A      187 VGCAStGDTSAALSAYCASAgIPSIVFLPankisxAQLVQPIANGAFVLSIDTDFDgCXKLIREITAEl---pIYLAns- 262
2D1F_A       88 VLCAStGNTSASAAAYAARAgITCAVLIPqgkiamGKLAQAVMHGAKIIQIDGNFDdCLELARKMAADfp--tISLVns- 164
gi 13488402 133 VATASsGNAGASLAAYAARAgLKCVVTTFags-agAMLAQIRKYGATVLPLVSKMDrWSLLEQAAARYd---wFIASpyh 208
gi 15789760 131 VALAStGNAGQSAGAYAGRAgLDAHVFLPsrs-gfTTKAMVNVHGGDMNVVQGRIGdANAAFEDAMDEhgeawYSLQaf- 208
gi 15790946 114 VVEDSsGNAGAAVAQYAARAgIDAEIYVPada-paAKLDAMEAAGATTVPVAGGRRaVTEACVSEAVGsd--aWYASha- 189
gi 18977552 114 IGTVStGNMAASVAAYGAHSeLEVYILVSsti-peEKIKALAPYGAKIVKVHGDYGeLYYRALEIGQKk---gIYFIns- 188
gi 27379888 198 MAMPTnGNAGAALAAYATSCgIKTTIFCPadt-peVNVSEIELQGATVYRVNGYIDdCGKIVGEGNAKvg--wFDTStl- 273
gi 28210306 125 VGAVStGNMAVSVAAYGQRAgFETFIFVGeni-peEKLNPVAIYGANLIKVEGDYGkLYYESLEIGKDq---gIYFIns- 199
gi 29829733 116 VVADSsGNAGTAIAAYCARAaLPCTVYVPegt-saKKLEQIEAHGARLRVVEGDREaTAHAAREAADEag--vFYAShv- 191
gi 32035415 114 VVQDSgGNAGNSVAAYCAKAgIQCEIFVPegt-spKKINMIEAHGAKANVIKGSRDhCADVCRAKVENeg--vYYANhv- 189
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1E5X_A      263 -----lnSLRLEGQKTAAIEILQQFdwqVPDWVIVPGGNLGNIYAFYKGFKXCQELGLVDr-IPRXVCAQAANANPLylh 336
2D1F_A      165 -----vnPVRIEGQKTAAFEIVDVLg-tAPDVHALPVGNAGNITAYWKGYTEYHQLGLIDk-LPRMLGTQAAGAAPLvlg 237
gi 13488402 209 gpvvgshPLGIEGYKTIAYEIVEQSdgtAPDWCVLPVCYGDALSGVWAGFCELFAQGSITr-LPRLVAAEVHGSLADala 287
gi 15789760 209 -----atPYRHEGKKTLLYETVEQLdweTPDHVVYPTGDGGGLVGMWKAATELRELGITAd-VPAMYAAQAEGCAPIvra 282
gi 15790946 190 -----wsPSFYAGTATVAYEIAAQRgwrAPDAVVVPVGHGTLLLGAYRGFRALAAGGWIEs-MPRLLGVQAAGVEPVaaa 263
gi 18977552 189 -----ddPFRVEGYKSISFEISEEI---TPDYVIVPTSSGGLFRGIVKGFLEAKNSGLIEk-LPRFFVVQAEGCSPIcka 259
gi 27379888 274 -----kePYRIEGKKTMGLELAEQLgwdVPDVIFYPTGGGTGLIGMWKAFDELEKIGFIGskRPRMVAVQASGCAPMvra 348
gi 28210306 200 -----dvTYRVEGYKTAAFEMCEQLnfnVPDYVVVPTSAGGNIRGILKGFKEFKEAGIIDr-VPKMICAQASGCSPIcns 273
gi 29829733 192 -----fnPYFLHGTKTYVHELWEDLggrLPDALVVPVGNGTLLLGAALAVAELHAAGLIDr-RPALYAVQAAAVAPLaha 265
gi 32035415 190 -----ynPFFYEGTKTYIYETFEQLg-rIPKHIVVPVGNGTLFIGVIKGLEHLLESGVIDe-MPTIIALQSEYCDPLlqa 262
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1E5X_A      337 yksg-wkdfkpXTASTTFASAIQIGDPVSIDRAVYALKKCnGIVEEATEEELXDAXAQADST-GXFICPHTGVALTALFK 414
2D1F_A      238 ---------epVSHPETIATAIRIGSPASWTSAVEAQQQSkGRFLAASDEEILAAYHLVARVeGVFVEPASAASIAGLLK 308
gi 13488402 288 sg----adavsERQMLFDSLAVSIGTPRSTFQALKALRESgGHAVPVSNHGLIAMQERLARDeGIFAELASVTPLIAISK 363
gi 15789760 283 fqeg-rdahepWDTPDTICGGIEIPDPAGSRLVLEALRESnGGAVAASDEEILEAGTAVASRaGPEMGATCAAAAAGAWE 361
gi 15790946 264 vn----gsasdEDAVNEAADGIQIRSPARLDQVAAAVAATdGDVVACDESATTDALASLHEA-GLYVEPTSAVAVAGLAA 338
gi 18977552 260 fnag-kekierFENPHTIAHAIENPYPPSGNAVLRLLKKLnGICTTVTDEEILEAQKILGRE-GIFVQLASATGIAAVKK 337
gi 27379888 349 fdag-tehatrWEDAHTIASGIRVPQAIGDFLILRAVRESkGFAIAVDDDKISAALNEVAREeGLLLCPEGAATYAAYKE 427
gi 28210306 274 fkeg-kdeierIMDPCTIAHGIENPFPPSGNDTLRKIRENgGTCVAVTDEEIIKAQKEMAKE-GIFVQPESCVPLASVKK 351
gi 29829733 266 weqgaddlvgvTPAAPTFAEGIAIPRPPRARQILRAVRESgGAFLTVTEDQIRHAQSDLASR-GLYVESTGVACWAAVRA 344
gi 32035415 263 vkkg-qkfaeqVEIKPTIAEGIAIGQPMRASEILAYAEKYaIRFVHAPEDKILAARNALAKK-GIYCEHTTAANYAAYLH 340
                       330       340
                ....*....|....*....|....*...
Feature 1                                   
1E5X_A      415 LRnqgv----iaptDRTVVVSTAHGLKF 438
2D1F_A      309 AIddgw----vargSTVVCTVTGNGLKD 332
gi 13488402 364 LRdqgi----iatpDRVVAVVTASGLKD 387
gi 15789760 362 LAdrda----fgedDTVVVVSTGTANKA 385
gi 15790946 339 YRargv----vgdsDDVVVALTGSGLKK 362
gi 18977552 338 LRrkgi----ikegAIVVSILTGSGLKT 361
gi 27379888 428 SLadgr----vsktDRVMLFNCATGLKY 451
gi 28210306 352 LKiegy----ldenDSVACVLTGSGLKY 375
gi 29829733 345 GAlpqprprpgegmPAAVVPLCGAGLKT 372
gi 32035415 341 YCely------gavEDCLITICGAGLKS 362

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