1JWL,1TLF,1LBI,2P9H,2PE5


Conserved Protein Domain Family
PBP1_LacI

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cd01574: PBP1_LacI 
Click on image for an interactive view with Cn3D
Ligand-binding domain of DNA transcription repressor LacI specific for lactose, a member of the LacI-GalR family of bacterial transcription regulators
Ligand-binding domain of DNA transcription repressor LacI specific for lactose, a member of the LacI-GalR family of bacterial transcription regulators. The ligand-binding domain of LacI is structurally homologous to the periplasmic sugar-binding domain of ABC-type transporters and both domains contain the type I periplasmic binding protein-like fold. The LacI-GalR family repressors are composed of two functional domains: an N-terminal HTH (helix-turn-helix) domain, which is responsible for the DNA-binding specificity, and a C-terminal ligand-binding domain, which is homologous to the type I periplasmic binding proteins. As also observed in the periplasmic binding proteins, the C-terminal domain of the bacterial transcription repressor undergoes a conformational change upon ligand binding which in turn changes the DNA binding affinity of the repressor.
Statistics
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PSSM-Id: 107259
View PSSM: cd01574
Aligned: 46 rows
Threshold Bit Score: 215.523
Threshold Setting Gi: 148656431
Created: 21-Jul-2004
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1JWL_A; Escherichia coli LacI bound to ONPF anti-inducer, contacts calculated at 3.5 A.
    View structure with Cn3D
  • Structure:2P9H_A; Escherichia coli LacI bound to IPTG inducer, contacts calculated at 3.5 A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #     ##  #                                             #                 
1JWL_A        63 LIGVATSSLAlHAPSQIVAAIKSRAdqlGASVVVSMversgveacktAVHNLLAQRVSGLIINyplddqda--iaveaac 140
gi 148656431  10 TLGCVLNDPRfTFWSILRHGIRSRAa--DHGIAVIDqaagaveaqveMVADLLRRRVHALILGpahetrafaqhlelarl 87
gi 119945696  62 MIGLVTLDILlHGASLLANGVRKQAknlGYGTSLSViadpaidkittAVDDLKAQRVEFIIMNiplssdna--emlvvqy 139
gi 77975758   65 TLGLATTDLSlHAPSQIAAAIKRKArelGYNVVIAMvddhsehacqqAINELLAQRVDGILVNvplkpkrs--qkiaqqc 142
gi 146310588  63 TLGLITTDLAlHAPSQIASAVKSRAgerGASVMISMveqpe--fcqaALQELLAQRVEGLLVNvplddaea--ekirhla 138
gi 84387231   62 SVGLVTLDMAlHGAAMIANGVRMQAhdmSYGVAFSVvsepnlantreAIRELMAQQVDSIILNvplesada--ellveqy 139
gi 49610955   65 TLGLVTTDLSlHAPSQIAAAIKSTAsqlGINIVMSMlsspdvntcnnAVNDLLSQRVSGVIVNvplstdev--ahisqtc 142
gi 156935127  94 TLGFITVSLAlHAPSQIAAAIKSHAaraDYSVVIATletgtleelqrSLNEMRAQKVDGVIINlplgaeaa--aslvndn 171
gi 125933     65 SIGLITASVTlHAPSQIAAAIKSHAslhQLEVAIAMpaqadfvalqaRLDELRAQHIRGVIVSlplesata--erlvqdn 142
gi 16121157   65 TMGLVTTSLAlHAPSQVAAAIKRYAhieGYQVLISMidesvnqsiqhSINDLKSQWVGKVIINvpletava--ekiaadn 142
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #             #                                 #   #                   
1JWL_A       141 TNVPALFLDvsdq--tpinSIIFSHEDGTRLGVEHLVa--lGHQQIALLAGPlssvSARLRLAGWHKYlt-rnQIQPIAE 215
gi 148656431  88 ARVPVIEVDggilegyvsaTVRSNERQGLMRVAELLVahlgDRMRIALIAGPrn-rRAEIMAEVLRQWp----GVQIVAQ 162
gi 119945696 140 SSIHFVFIDvppd--sqvnSVSGASYNGAKIAAELMLs--lCRKRLLFINGPqgssASRLRLQGWLDVlkedaDSKIINQ 215
gi 77975758  143 VDTPALFLDvdpq--saafSVLFDPKLGAIQGAEHLIa--lGHQQIVFICGPketiSAQLRCQGWLQAls-taSLVPQAV 217
gi 146310588 139 APVPVLFFDvsps--anvhRLAFDAEQGATLGAEHLLs--lGHQHIALLAGPessvSSRARLSGWQARlg-qaNVTPCAI 213
gi 84387231  140 QHLNLIFIDvpsn--sqvnYVCGDHAEGAKLAAQHLLe--sGRTDFLLITGPnessASKIRHQSWLAAls-daESKVQYQ 214
gi 49610955  143 ADTPVLFMDadph--tdilNVMFDPDHGARLAIAHLVe--lGHQHIALLTGPmtsiSARLRYEGWLAEle-kqRLSPVSV 217
gi 156935127 172 SDLLCLFLDvpad--sdvfHVSFDPRDGSRQSVELLLn--gGHQRIALLAGPehsvASGMRLACWQEVls-ahGLAPCAV 246
gi 125933    143 PDMACLFLDvspe--advcCVRFDHRDGCGACVRHLWe--mGHREFGLLAGPessvSARLRLASWREAlh-slNIARSTT 217
gi 16121157  143 DDVTCLFLDvdpy--ssvfNVSFNPADGTRASVKYLYe--lGHREIALLAGPkqsvSANLRLKSWLETla-dyGLSPVSV 217
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      #                           #                     
1JWL_A       216 REGDWSAMSGFQQTMQMLNegivptAMLVANDQMALGAMRAITESGlrVGADISVVGYDDTedss---cyiPPLTTIKQD 292
gi 148656431 163 STGDWTRDSGRRLAEEWLRtgqpidAIFAANDPMALGVVDAVEALN--LTGSIAVVGFDGLpealrainsgAMLATVNQD 240
gi 119945696 216 FEGDWRASSGYRCMRQALSdghdfdGVLVANDQMALGVLRALSEFNlsVPQQVSVIGFDDTvdsa---yftPPLTTIKQD 292
gi 77975758  218 YYGEWNAQSGYQATQKLLIsntpfsAILVANDQMALGVLRALHEHNidVPGQVSVIGYDDTadsa---yfqPPLTTIRQN 294
gi 146310588 214 AHGDWSAASGYEKGHQLLSgatlpdAILVANDQMALGVMRACSEKGiaVPGQISVVGFDDTadsa---wfsPPLTTIRQA 290
gi 84387231  215 YQGNWQAESGYLGVREAVAkqavfdAVLVASDQMALGALRALQELQipVPDKVAVVGFDGIedsa---ffnPPLTTIKQD 291
gi 49610955  218 LHGDWSAASGYHQTLALLGqslrisAIVVANDQMALGVLRALHEYGlrVPEQMSVIGFDDTqdsa---yytPPLTTIRQD 294
gi 156935127 247 LHGDWSSKDAWQQTLSLFRtrrditALVVANDQMAFGAMSALDELGlrVPQDVSVIGYDDTpdsa---yfiPPLTTVEQD 323
gi 125933    218 VFGDWSAASGWQKTFELLHlqprisAIVVANDQMALGVLSALAQLNrsGSQAVSVTGYDDTadsl---yfqPPLTTVAQD 294
gi 16121157  218 IHGNWDAQSGYSGALQMLRetpqfsAVLVANDQMALGVLSAFHQNQlaVPGEKSVIGYDDTyess---ffyPALTTVSLD 294
                        250       260       270
                 ....*....|....*....|....*....|....*...
Feature 1                                              
1JWL_A       293 FRLLGQTSVDRllqlsq--gqavkGNQLLPVSLVKRKT 328
gi 148656431 241 PLCIGQQAVDTalqalr--sepveRQILTPGELITRDT 276
gi 119945696 293 FLTIGERAVLLafsvad-kakksyRHESIVTELIERKS 329
gi 77975758  295 FKLLGEESVSRlivrlhhtieppsESLLLATELTIRQT 332
gi 146310588 291 FRDAGERSVEWllssa----tnelWQTQLPVTLVARHS 324
gi 84387231  292 FTTIGRQAVILaeklna-nsqdtlLQHHIETTLLPRES 328
gi 49610955  295 FRLLGKEGVTRllatlr--dptqaTSLLLPTELIVRHS 330
gi 156935127 324 FNRLGAQAVSRmvarlt--ghgesGSTLLPTRFIERRS 359
gi 125933    295 FDLLGKRAVERlialma--apqlrIRELLPTRLIVRQS 330
gi 16121157  295 LDLQGKEAVRRmlsasd--nesmrSSSILPAKLVVRNS 330

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