4FYP


Conserved Protein Domain Family
HAD_VSP_like

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cd01624: HAD_VSP_like 
Click on image for an interactive view with Cn3D
vegetative storage proteins and related proteins, similar to soybean VSPalpha and VSPbeta proteins; belongs to the haloacid dehalogenase-like superfamily
Soybean [Glycine max (L.) Merr.] vegetative storage protein VSPalpha and VSPbeta levels were identified as storage proteins due to their abundance and pattern of expression in plant tissues, they accumulate to almost one-half the amount of soluble leaf protein when soybean plants are continually depodded. They possess acid phosphatase activity which appears to be low compared to several other plant acid phosphatases; it increases in the leaves of depodded soybean plants, but to no more than 0.1% of the total acid phosphatase activity in these leaves. This acid phosphatase activity has maximal activity at pH 5.0 - 5.5, and can liberate Pi from different substrates such as napthyl acid phosphate, carboxyphenyl phosphate, sugar-phosphates, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, phosphoenolpyruvate, ATP, ADP, PPi, and short chain polyphosphates; they cleave phosphoenolpyruvate, ATP, ADP, PPI, and polyphosphates most efficiently. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Soybean VSPalpha and VSPbeta lack this active site aspartate, other members of this family have this aspartate and may be more active. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319765
View PSSM: cd01624
Aligned: 5 rows
Threshold Bit Score: 188.908
Threshold Setting Gi: 21221800
Created: 12-Dec-2003
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              #####                                                     
4FYP_A        87 KEAYFYAkglalkndtVNVWIFDLDDTLLSSipyyakygy---gtentapgAYWSWLESgest----pglPETLHLYENL 159
gi 21221800   98 KERIAGSap-----geKQAVVLDIDNTSLETd------------------fGFSYPQPAnr-------pvLEVAEYAQEH 147
gi 300786505  34 AAPAAVSaaa----paYATWIADVTAVTTPAadylagrldvpggrtaivldIDNTSLETyysggittpavKPVLALAELA 109
gi 21220366   88 DRAARTAhhk----htRPAVVFDIDDTLLLSldyekkn------nygydgaTWAAYVDRadr-----pavPGSPELARYA 152
gi 56707326   26 AKKIIQAqqs----lqGNAIVLDIDETALNHyyslklag----fpqgenhtIWNELLSRtda-----ypiKATLDFYLYC 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  ##                                                       #            
4FYP_A       160 lelGIEPIIISDRwkKLSEVTVENLKAVGVTk---wKHLILKpngsk--------------ltqvVYKSKVRNSLVkkg- 221
gi 21221800  148 ---GVALFFVTARpgIIEAPTEWNLAHAGYEs----SGLYVRgfldl-------------fkdvaEYKTEQRAEIEskg- 206
gi 300786505 110 kskGAAVFFVSDRteLLRWPTEGNLKAVGYPv----DGLYLRplfnf--------------dpvqANKTKARAAIEqag- 170
gi 21220366  153 dkkGVEVFYNSGLgeAQRAAAVENLKKAGADvnldaSHMFLKdkanppsylahcatpgswtcttvQYKAGTRRHIErdlg 232
gi 56707326   93 ltsGLKVFFISARfaQYLESTKQALRNAGYVn---fEDVFVFpenieqy----------nskdfkNFKAERRAYIEslg- 158
                        170       180       190
                 ....*....|....*....|....*....|
Feature 1               ##  ##                 
4FYP_A       222 ynIVGNIGDQWADLVEDTPGRVFKLPNPLY 251
gi 21221800  207 ytIIANIGNSASDLSGGHAEKTFKLPDYDG 236
gi 300786505 171 ytIVANVGNNRTDLDGGHAERTFKLPDYDG 200
gi 21220366  233 ydVVANFGDQYSDLQGGYADRTYKLPNPTY 262
gi 56707326  159 ykILISIGDQSSDLLGGYTLYTLQLPNYLY 188

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