2HUP


Conserved Protein Domain Family
Rab19

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cd01864: Rab19 
Click on image for an interactive view with Cn3D
Rab GTPase family 19 (Rab19)
Rab19 subfamily. Rab19 proteins are associated with Golgi stacks. Similarity analysis indicated that Rab41 is closely related to Rab19. However, the function of these Rabs is not yet characterized. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 133267
View PSSM: cd01864
Aligned: 9 rows
Threshold Bit Score: 309.364
Threshold Setting Gi: 17543864
Created: 6-Jan-2012
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II
  • Structure:2HUP: Human Rab43 in complex with GDP; contacts at 4.0A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  #######         ##    ##                                             
2HUP_A       27 DFLFKLVLVGDASVGKTCVVQRFKTGAFSERQGSTIGVDFTMKTLEi--------------------------------- 73
gi 17543864   8 DYLFKIVLVGDMGVGKTCVVQRFRNGTFVDRQGTTIGVDFTMKTLVv--------------------------------- 54
gi 67678420  13 DFLFKLILIGDAGVGKTCVVQRFRSGVFAERQGSTIGVDFTMKTLEi--------------------------------- 59
gi 47211718  13 DFLFKIILVGDSNVGKTCVVQSFKSGIFMEKQQNTIGVDFTVRTIDi--------------------------------- 59
gi 47222068  13 DFLFKIILIGDSNVGKTCVVQNFKSGTFSAKQQNTIGVDFSVRTVEi--------------------------------- 59
gi 51094782  15 DYLFKIILIGDSNVGKTCVVQHFKSGVYTETQQNTIGVDFTVRSLDidgkkvknssasiitfasiqihgqtqkmspqiwt 94
gi 50234889  16 DFLFKLVLVGDASVGKTCVVQRFKTGAFSERQGSTIGVDFTMKTLEi--------------------------------- 62
gi 80476372  13 DFLFKIILIGDSNVGKTCVVHRFQSGIFMDNQQNTIGVDFTVRSLNi--------------------------------- 59
gi 2313047   19 DFLFKIVLIGDCGTGKTCIVDRFKTGNYIERHGNTIGVDFSMKTIAv--------------------------------- 65
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                                    
2HUP_A       74 --------------qGKRVKLQIWDTAGQERFRTITQSYYRSANGAILAYDITKRSSFLSVPHWIEDVRKYAGSNIVQLL 139
gi 17543864  55 --------------dGKRVKLQIWDTGGQERFRTITQSYYRSANGIVLCYDITCKQSFGSLQRWIDDVSKFAAPNVVKLL 120
gi 67678420  60 --------------qGKRVKLQIWDTAGQERFRTITQSYYRSANGAIIAYDITKRKSFASVPRWIEDVKKYAGSNIVQLL 125
gi 47211718  60 --------------dGKKVKMQVWDTAGQERFRTITQSYYRSAHGAMVAYDITRRSTFESVSHWIREVEHYGASSVVLIL 125
gi 47222068  60 --------------eGKKVKIQVWDTAGQERFRTITQSYYRSAHGAIIAYDITRHATFDSVSHWIKEVELYGASNVVLVL 125
gi 51094782  95 ksshylwepliwtllPVLLQMQVWDTAGQERFRTITQSYYRSAHAAIIAYDLTRRSTFESIPHWIHEIEKYGAANVVIML 174
gi 50234889  63 --------------qGKRVKLQIWDTAGQERFRTITQSYYRSANGAILAYDITKRSSFLSVPHWIEDVRKYAGSNIVQLL 128
gi 80476372  60 --------------nGKKVKVQVWDTAGQERFRTITQSYYRSAHGAIIAYDITRRQSFESVPHWIYEAGKYGAANLMLML 125
gi 2313047   66 --------------eGKQIKLQIWDTAGQERFRTITQSYYRSANGVLIVYDITKRSSFSNLQKWIEEVRRYTASNVLIIL 131
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ## #                            ###                                           
2HUP_A      140 IGNKSDLSELREVSLAEAQSLAEHYdi-lCAIETSAKd---------------------------SSNVEEAFLRVATEL 191
gi 17543864 121 IGTKCDLEDQRAIEAEEAEMLQRANgm-fAMLETSAKg---------------------------NVNVDNAFLELATIL 172
gi 67678420 126 IGNKSDLHESREVQLREAETLARHFdi-pCAIETSAKd---------------------------SSNVEEAFEKMATEL 177
gi 47211718 126 IGNKSDLQAQRQVLFEDACTLAESNgv-lAVLETSAKe---------------------------AQNVEAAFTLMAREL 177
gi 47222068 126 IGNKCDLEQEREVTFEDACNLAKNKem-lAALETSAKvirvhpsqgsvganqswcltfsswcpqeSQNVDEAFMMMAREL 204
gi 51094782 175 IGNKCDLWEKRHVLFEDACTLAEKYgl-lAVLETSAKe---------------------------SKNIEEVFVLMAKEL 226
gi 50234889 129 IGNKSDLSELREVSLAEAQSLAEHYdi-lCAIETSAKd---------------------------SSNVEEAFLRVATEL 180
gi 80476372 126 MGNKSDLAEKRQILFEEACTLAEKHgl-lAVLETSAKe---------------------------SHNVDEVFLLMAKEL 177
gi 2313047  132 VGNKCDLEEQREVDFEEARQMCQYIpeilFVMETSAKe---------------------------NMNVEDAFRCLANEL 184

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