Conserved Protein Domain Family
PurL

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cd02193: PurL 
Click on image for an interactive view with Cn3D
Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to formylglycinamidine ribonucleotide (FGAM), ADP, phosphate, and glutamate in the fourth step of the purine biosynthetic pathway. In eukaryotes and Gram-negative bacteria, FGAR-AT is encoded by the purL gene as a multidomain protein with a molecular mass of about 140 kDa. In Gram-positive bacteria and archaea FGAR-AT is a complex of three proteins: PurS, PurL, and PurQ. PurL itself contains two tandem N- and C-terminal domains (four domains altogether). The N-terminal domains bind ATP and are related to the ATP-binding domains of HypE, ThiL, SelD and PurM.
Statistics
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PSSM-Id: 100029
View PSSM: cd02193
Aligned: 3 rows
Threshold Bit Score: 350.829
Threshold Setting Gi: 49259562
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP bindingdimerization
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1T3T; Salmonella FGAR-AT with bound ADP, 3.5 A
    View structure with Cn3D
  • Comment:The N- and C-terminal repeats of PurL bind ATP/ADP

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 #   #                                                
1T3T_A     295 AHILMKVETHNHPTAISPWPGAATGSGGEIRDEGatgr-gakpKAGLVGFSVsnlripgfeqpweedfgkperivtaldI 373
1T3T_A     699 YGEAMSIGERAPVALLDFAASARLAVGEALTNIAatqigdikrIKLSANWMAaaghp------------------gedaG 760
1VK3_A      75 YSVAFKIESHNHPSAIEPYNGAATGVGGIIRDVLaxg---arpTAIFDSLHXs-------------------------rI 126
                       90       100       110       120       130       140       150       160
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           ###                                                        
1T3T_A     374 MTEGPLGGA-AFNNEFGRpALTGYFRTyeekvnshngeelrgyhkPIMLAGGIGNIRadhvqk--geivvGAKLIVLGgp 450
1T3T_A     761 LYDAVKAVGeELCPQLGLtIPVGKDSMsmktrwqegneqremtspLSLVISAFARVEdvrhtltpqlsteDNALLLIDlg 840
1VK3_A     127 IDGIIEGIA-DYGNSIGVpTVGGELRIssly-----------ahnPLVNVLAAGVVRndxlvd-skasrpGQVIVIFGga 193
                      170       180       190       200       210       220       230       240
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                      
1T3T_A     451 amniglgggaassmasgqsdadldfasvqrdnpeMERRCQEVIDRCwqlgdanpilFIHDVGagglsnAMPELVsdggRG 530
1T3T_A     841 kghnalgatalaqv-----yrqlgdkpadvrdvaQLKGFYDAMQALvaar---kllAWHDRSdggllvTLAEMAfaghCG 912
1VK3_A     194 tgrdgihgasfased--ltgdkatklsiqvgdpfAEKXLIEAFLEXveeg---lveGAQDLGaggvlsATSELVakgnLG 268
                      250       260       270       280       290       300
               ....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                  
1T3T_A     531 GKFELrdilsdepgmspleiwcnesQERYVLAVAadqlplfde--lckrerapYAVIGDA 588
1T3T_A     913 VQVDIaalgddh------laalfneELGGVIQVRaedrdaveallaqygladcVHYLGQA 966
1VK3_A     269 AIVHLdrvplrepdxepweilisesQERXAVVTSpqkasrile--iarkhllfGDVVAEV 326

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