Conserved Protein Domain Family
ThiL

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cd02194: ThiL 
Click on image for an interactive view with Cn3D
ThiL (Thiamine-monophosphate kinase) plays a dual role in de novo biosynthesis and in salvage of exogenous thiamine. Thiamine salvage occurs in two steps, with thiamine kinase catalyzing the formation of thiamine phosphate, and ThiL catalyzing the conversion of this intermediate to thiamine pyrophosphate. The N-terminal domain of ThiL binds ATP and is related to the ATP-binding domains of hydrogen expression/formation protein HypE, the AIR synthases, FGAM synthase and selenophosphate synthetase (SelD).
Statistics
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PSSM-Id: 100030
View PSSM: cd02194
Aligned: 197 rows
Threshold Bit Score: 157.715
Threshold Setting Gi: 170933865
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP bindingdimerization
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #                         ###                                            # 
1VQV_B         7 GEFGLIDLIKktle-------------skviGDDTApve-ycsKKLLLTTDVLNEGVHFlrsyipeAVGWKAISVNVSDV 72
3C9R_A        43 GEFGLIDLIKktle-------------skviGDDTApve-ycsKKLLLTTDVLNEGVHFlrsyipeAVGWKAISVNVSDV 108
gi 110622645   7 GEIGLIRRIGkilq-------------spylGDDCAvid-ngnDYLVVTTDMLHRVTDFpremtgeDIGWMSVAVSLSDV 72
gi 20088968   12 GERALISRLSgifntpegkergqegilvgagSDDCAvldlkgeDCLVVTTDMLHRTTDFppemtpwQMGWMSAAVNLSDI 91
gi 116754398  24 GERSLISKITevlg--------------gleHDDCAvve-ldgDYLVVTTDMLHRETDFpdqatpwQIGWMSAAVNLSDI 88
gi 88603843    2 DDRDLLEIIRpvcg-------------sdtcSDDCAilt-lpcGALVTSTDMLHERSDFpagmtgwQIGWMSVAVTISDI 67
gi 124485263   2 DDRSLLDSIRhlig-------------edetADDCAafdlldgRILVSSTDMLHETTDFpkgmtefEKGWMSAAVTLSDI 68
gi 126178430   2 DERGLHRLIGtiid-------------perlDDDCAvip-cgdRLMVASTDMLHETTDFpagmtdwQIGWMATAVTLSDV 67
gi 153894412   2 NERDALRALTadl---------------phaGDDAAv-----vDDTVITTDMLHERTDFppgttryTAGWRVVGASLSDV 61
gi 110667077   2 DERDALGQIAktl---------------pqaGDDAAvis-ldsVSLVITTDMLHETTDFpdgttryTAGWRAVGASLSDI 65
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #                #               ###                        #        
1VQV_B        73 IANGGLPKWALISLNLpedlevsYVERFYIGVKRACEFYKCEVVGGNISKsekIGISVFLVGETer--fVGRDGAr-lGD 149
3C9R_A       109 IANGGLPKWALISLNLpedlevsYVERFYIGVKRACEFYKCEVVGGNISKsekIGISVFLVGETer--fVGRDGAr-lGD 185
gi 110622645  73 AGMGARPVGVVAAIGIpgdtdieFIDGIATGMRNCAKCYGTDIIGGDTDQhqeLTIVTTAFGRVnkdqvKLRSGAk-vGD 151
gi 20088968   92 AAMGAEPTGLLMAIGIpadteiaFIEELAKGIQACAEFCETAVIGGDLDThaeLTITGTALGRVkkshlLLRKGAk-pGD 170
gi 116754398  89 AAMGAEPLGVLIASGIppetdleFVEEIFEGIRDCVKAHGTEVLGGDTDAhreLTICGTALGRVsreriLRRRGAr-pGD 167
gi 88603843   68 AAMGAEPLQIVLAIGLdt---eeRLAEIVLGAQACCDTYGAVYAGGDLDAhseLTIVSTGVGIIrd-gePVRRGGakpGD 143
gi 124485263  69 ASCGAKPVQLLVAVGLdd---psRLVPFMEGAVSCAKTFGAKVSGGDIDShieLTVVTTGFGIVek-thYCRRSGaraGD 144
gi 126178430  68 ASMGAAPGEVLLAVGLdr---peRLSGIMEGARDCCTRFGAELVGGDLDAhreLTIVSTGLGLVapehlVRRRGAr-pGD 143
gi 153894412  62 AAMGAAATAAVAVYADaaf-degDLDRFVAGAIDVCEAVDAEYVGGDLDThdeFTTASTAIGDVtdagvVTRSGAr-pGD 139
gi 110667077  66 AAMGGQATAAVAVYGApaf-dpdEIDSFIAGAIDVCDAANAQYVGGDLDTtseLTIATTALGTVth--pSYRHGAr-iGD 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                   # ##                 
1VQV_B       150 SVFVSGTLGDsraglelllxekeeyepfelaliqrhlrPTARIDYVKHIq--kYANASXDISDGlvADANHLAQRSgVKI 227
3C9R_A       186 SVFVSGTLGDsraglelllmekeeyepfelaliqrhlrPTARIDYVKHIq--kYANASMDISDGlvADANHLAQRSgVKI 263
gi 110622645 152 LLCVTGYLGTtaagfkvltgereaspedrqilfrsffrPEPRLGEGMILaqypGVTAMMDVSDGlgKSVYELSVASkVGF 231
gi 20088968  171 LVCVTGHTGSagaaleaiqskk----svsenvlkalfePVPRTKEARKLaeseAVTSMMDTSDGlaMSLYDLARQSkVGF 246
gi 116754398 168 LLCTTGSLGGagaglkalkig-------ridlarrllePCPRVREGIALagtgAVTAMMDNSDGlaLSLHDLAEAGgTGF 240
gi 88603843  144 IICVTGTLGRailgltgds-----------rfwkdlcePQPRVREGTVArl-aGAHAMMDISDGlaISLYDIAAEShVGM 211
gi 124485263 145 LVCITGTPGLamaalegde-----------ryrknlltPIPQVKAGQKIaa-aGASSMMDVSDGlsISLFDMSEASnVGF 212
gi 126178430 144 VIAVTGPLGEaeaalagyd-----------rhrrellePQPRVREGQALgr-aGVSAMMDISDGlvLSLHDLLAVNdCGF 211
gi 153894412 140 ALCVTGEWGRsaaalrlfkrgd----deaverandlfrFTPRVADGLALa--dRATAMMDSSDGlaRSLHQLAEASgVGF 213
gi 110667077 142 CICVTGNLGRtaaglkafetg-------diahanelfrFTPRVNTGAALa--kHTTAMMDSSDGlaRSVHQLASASeVGM 212
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                                                                        
1VQV_B       228 EILSeklplsnelkxycekygknpieyaLFGGEDYQLLFTHpk-----erWNPfldXTEIGRVE 286
3C9R_A       264 EILSeklplsnelkmycekygknpieyaLFGGEDYQLLFTHpk-----erWNPfldMTEIGRVE 322
gi 110622645 232 IVYAdalpvreearkv-arsrtelldmaICFGGEFELLFTVnpd---evgNIKgvdFTVIGKVT 291
gi 20088968  247 RIREealpilpevedf-tsdpdellefaLYTGGDFELIFTVdpgr--lkkVQNicnLTIIGECT 307
gi 116754398 241 LVECeripiaeevrai-aesddealemvLRAGGDFELLLTVredm--iddARKaceLTVIGRAV 301
gi 88603843  212 EISSgriplppea------dvqvaleaaLYGGGDFELLFFIsee----kmKNLtipVTQIGRVS 265
gi 124485263 213 ALESakfnlpdvr-------agsareyyLYGGGDFGLLFCIsre----rlPALdaeYTVIGKVV 265
gi 126178430 212 SVDTarlplpagv------pedegrelaLYGGGDFELLFTAppd----ilPVPgveARVIGEVI 265
gi 153894412 214 ELDPdrvpvhpavdav-addpaerfelaAHFGEDFELVCTLpddavepvsETCpggLTRVGRVV 276
gi 110667077 213 SLHWdaipthpdvkey-vddvstrreltTTVGEDFELVLTLpsselanarETTsvpLHVIGEVT 275

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