Conserved Protein Domain Family
PurL_repeat1

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cd02203: PurL_repeat1 
Click on image for an interactive view with Cn3D
PurL subunit of the formylglycinamide ribonucleotide amidotransferase (FGAR-AT), first repeat. FGAR-AT catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to formylglycinamidine ribonucleotide (FGAM), ADP, phosphate, and glutamate in the fourth step of the purine biosynthetic pathway. In eukaryotes and Gram-negative bacteria, FGAR-AT is encoded by the purL gene as a multidomain protein with a molecular mass of about 140 kDa. In Gram-positive bacteria and archaea FGAR-AT is a complex of three proteins: PurS, PurL, and PurQ. PurL itself contains two tandem N- and C-terminal domains (four domains altogether). The N-terminal domains bind ATP and are related to the ATP-binding domains of HypE, ThiL, SelD and PurM.
Statistics
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PSSM-Id: 100034
View PSSM: cd02203
Aligned: 120 rows
Threshold Bit Score: 336.366
Threshold Setting Gi: 32490886
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP bindingdimerization
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1T3T; Salmonella FGAR-AT with bound ATP, 3.5 A
    View structure with Cn3D
  • Comment:The N- and C-terminal repeats of PurL bind ATP/ADP

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1VK3_A         33 ELQAFSVXWSEHCGYSht------------kKYIRRLpktgfegna---------------------------------- 66
1T3T_A        213 ELYMFAQANSEHCRHKifnadwiidgkpqpkSLFKMIkntfettpdyvlsaykdnaavme-------------------- 272
gi 38234679   211 ELAALDTYWSDHCRHTtf------------nTELTEItnsssrygaqldralkrydelreangrahkprtlmdlgt---- 274
gi 154496429  218 ELRVVDTYWSDHCRHTtf------------gTHIDKAeilddevaqayqeylaareevygpekaavrpqtlmdmat---- 281
gi 18309662   213 EIKVIDTYWSDHCRHTtf------------eTIIKDVyieegkysepikkayedyknsrayvygenlnnkevklmdl-at 279
gi 169825056  621 EIKILDTYWSDHCRHTtfntfld--iefnakTKLDEAihnsfeeylkvreelkiekpinlmsfgtilskymr-------- 690
gi 19704325   215 EIKVLDTYWSDHCRHTtf------------eTKINKVtfpdsefgkqmekefndylklkedvskkrdvslmdmativaky 282
gi 167752776  208 ELRILDTYWSDHCRHTtf------------tTELENItveesfvkdeiegtlalylkirrelgregksiclmdlat---- 271
gi 19745220   230 ELKVLDTYWSDHCRHTtf------------eTELKNIdfsaskfqkqlqttydkyiamrdelgrsekpqtlmdmat---- 293
gi 160914854  209 ELKVLDTYWSDHCRHTtf------------eTVLQNIkfdagslqdsmqkayelylklreevhggkkvmtlmdmat---- 272
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                          #   #          
1VK3_A         67 ------------------------------gvvnlddyYSVAFKIESHNHPSAIEPYNGAATGVGGIIRDVLAX--GARP 114
1T3T_A        273 ----------------gsavgryfadhntgrydfhqepAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATgrGAKP 336
gi 38234679   275 imvrelrrngtmtdqevseeinacsvyvdvdtpegdkpWLLMFKNETHNHPTEIEPFGGASTCLGGAIRDPLSG--RSWV 352
gi 154496429  282 lgakvlkkrgvltgldeseeinacsvhvtatvdgeeqdWLLMFKNETHNHPTEIEPFGGAATCIGGAIRDPLSG--RAYV 359
gi 18309662   280 iamkelrkrgklddldvseeinacsinieietdkgteeYLLMFKNETHNHPTEIEPFGGAATCLGGAIRDPLSG--RSYV 357
gi 169825056  691 ---sknefddleisseinacsvkikvrvevngvdetrdYLLMFKNETHNHPTEIEPFGGASTCLGGAIRDPLSG--RAFV 765
gi 19704325   283 lkkegkldnlevseennacsvyvdvevedfegkkaiekWLLMFKNETHNHPTEIEPFGGASTCLGGAIRDPLSG--RAYV 360
gi 167752776  272 igarylrskgllddlevseennacsvfvdvdvddrtekWLLQFKNETHNHPTEIEPFGGASTCLGGAIRDPLSG--RSYV 349
gi 19745220   294 ifgryerangrlddmevsdeinacsveievdvdgvkepWLLMFKNETHNHPTEIEPFGGAATCIGGAIRDPLSG--RSYV 371
gi 160914854  273 iagkylrkqgklddmeisdeinacsieisvdvdgkeepWLLMFKNETHNHPTEIEPFGGASTCIGGAIRDPLSG--RSYV 350
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                  ###                    
1VK3_A        115 TAIFDSLHXs-------------------------rIIDGIIEGIADYGNSIGVPTVGGELRIssly-----------ah 158
1T3T_A        337 KAGLVGFSVsnlripgfeqpweedfgkperivtaldIMTEGPLGGAAFNNEFGRPALTGYFRTyeekvnshngeelrgyh 416
gi 38234679   353 YQAMRISGAgdintprtet--------idgklpqadISARATHGYSSYGNQIGLATTSVRELIhpgy-----------va 413
gi 154496429  360 YQAMRVTGCgdprtpldqt--------lsgklsqrkIAQTAAAGYSSYGNQIGLATGQVSEIYhpgy-----------va 420
gi 18309662   358 YQAMRVTGSadptveicet--------lkgklpqrkITLGAAHGYSSYGNQIGLATGQVSEIYhpny-----------aa 418
gi 169825056  766 YQAMRITGAgnpltsledt--------mdgklpqikITQEAAKGYSSYGNQIGLATGFVDEIYhdgy-----------va 826
gi 19704325   361 YQAIRVTGSgnpletveet--------lkgklfqkkITTGAASGYASYGNQIGIATSLVSEIYhdgy-----------ka 421
gi 167752776  350 YQAMRVTGAgniyqpvset--------lpgklpqrvISLKAAAGYSSYGNQIGLATTHVREVYhpdy-----------va 410
gi 19745220   372 YQAMRISGAgdittpiaet--------ragklpqqvISKTAAHGYSSYGNQIGLATTYVREYFhpgf-----------va 432
gi 160914854  351 YQAMRISGAgditkdiadt--------lpnklpqsrISKGAAAGYSSYGNQIGLATTYVKEIFhdgy-----------va 411
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1VK3_A        159 nPLVNVLAAGVVRNdxlvdskasrpGQVIVIFGGATGRDGIHGAsfasedlt--gdkatklsIQVGDPFAEKXLIEAFLE 236
1T3T_A        417 kPIMLAGGIGNIRAdhvqk-geivvGAKLIVLGGPAMNIGLGGGaassmasgqsdadldfasVQRDNPEMERRCQEVIDR 495
gi 38234679   414 kRMELGAVVAAAPQenvkr-lepqpGDVVIILGGRTGRDGVGGAtgsskahdeeslarsgaeVQKGNPVNERKIQRLFRR 492
gi 154496429  421 kHLELGAVVAAAPAehvvr-ecpapGDVVILLGGRTGRDGIGGAtgsskshnlesldtmaseVQKGNAPEERKIQRLFRR 499
gi 18309662   419 kRMEVGAVIAATPKenvir-lepskGDIVILLGGRTGRDGIGGAtgsskehteesinqcgaeVQKGNAPTERKIQRLFRN 497
gi 169825056  827 kRLECGAVMAAAPIenvkr-iepekGDIVILLGGRTGRDGIGGAtgsskshkvtsiktesaqVQKGNAPEERKIQRLFRI 905
gi 19704325   422 kRMEVGAVVAAAPVenvvr-kspvpTDSIIIIGGKTGRDGCGGAtgsskehndkslllcgaeVQKGNAPEERKIQRLFRN 500
gi 167752776  411 kRLEVGAVVGAVKAenvrr-espvpGDVVLLLGGRTGRDGVGGAtgsskehtdkslemcgseVQKGNAPEERKLERLFRR 489
gi 19745220   433 kRMELGAVVGAAPKenvvr-ekpeaGDVVILLGGKTGRDGVGGAtgsskvqtvesvetagaeVQKGNAIEERKIQRLFRD 511
gi 160914854  412 kRMEVGAVVGAAPKenvvr-kspetGDIVVLIGGATGRDGVGGAtgsskehndtsltkcsseVQKGNAPIERKLQRLFRN 490
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1VK3_A        237 Xve---egLVEGAQDLGAGGVLSATSELVakgnLGAIVHLDrvplrepdxepwEILISESQERXAVVTSpqkASRILEIA 313
1T3T_A        496 CwqlgdanPILFIHDVGAGGLSNAMPELVsdggRGGKFELRdilsdepgmsplEIWCNESQERYVLAVAadqLPLFDELC 575
gi 38234679   493 Pei---aqMIVRCNDFGAGGVSVAVGELT----DSIDIHLDrvplkyaglnarEIAISESQERMAVVVRpedAAAFIEAA 565
gi 154496429  500 Gev---trLIKRCNDFGAGGVSVAIGELA----DGLSISLDavrkkydgldgtELAISESQERMAVVVApqdAAAFIAAA 572
gi 18309662   498 Kev---aqMIKRCNDFGAGGVSVAIGELC----RGIDIDLNkvpkkyegldgtELAISESQERMAVVISsenADRFIKLS 570
gi 169825056  906 Pel---asLIKKCNDFGAGGVSVAIGELH----DGVDIYLDrvplkykglkpfEIAISESQERMACVIAkkdLEEFVKYA 978
gi 19704325   501 Pna---tkLIKKCNDFGAGGVSVAIGELA----DGVEVNLDlvpvkyeglngtELAISESQERMAVIVSkedTEKFLKYV 573
gi 167752776  490 Pev---trLIKKSNDFGAGGVSVAIGELT----DGLDIYLDrvptkysglnatELAISESQERMAVVIEasaEEEFKRYC 562
gi 19745220   512 Gev---trLIKKSNDFGAGGVCVAIGELA----DGLEIDLDkvplkyqglngtEIAISESQERMSVVVRpsdVDAFIAAC 584
gi 160914854  491 Pka---trLIKKANDFGAGGVSVAVGEIA----DGLLIDLDkvpvkysglsgtELAISESQERMAVLIEkdaFEEFRQLA 563
                         410       420       430
                  ....*....|....*....|....*....|...
Feature 1                                          
1VK3_A        314 RkhllFGDVVAEVieepvyRVXYrn--dlVXEV 344
1T3T_A        576 KreraPYAVIGDAteeqhlSLHDnhfdnqPIDL 608
gi 38234679   566 AteniEAVELAIVtdsgrlRMFLge--dvVLDL 596
gi 154496429  573 EsenlEAYQVATVtedprmVMTWng--qtVANL 603
gi 18309662   571 EeenlEATIVAEVtdtdrlRMNWkd--ktIVDI 601
gi 169825056  979 DaeniETTVVADItdtnrmRMFYde--dlIADI 1009
gi 19704325   574 DeenlLGTVVGYVtdknrlTLNWkg--kaIVDI 604
gi 167752776  563 AeeniEVTHVADVtdsrrmRMYNkg--klVVDL 593
gi 19745220   585 NkeniDAVVVATVtekpnlVMTWng--etIVDL 615
gi 160914854  564 YeenlDSCIVAEVteekrlVMTFhg--eeIVNI 594

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