1T3T,1VK3


Conserved Protein Domain Family
PurL_repeat2

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cd02204: PurL_repeat2 
Click on image for an interactive view with Cn3D
PurL subunit of the formylglycinamide ribonucleotide amidotransferase (FGAR-AT), second repeat. FGAR-AT catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to formylglycinamidine ribonucleotide (FGAM), ADP, phosphate, and glutamate in the fourth step of the purine biosynthetic pathway. In eukaryotes and Gram-negative bacteria, FGAR-AT is encoded by the purL gene as a multidomain protein with a molecular mass of about 140 kDa. In Gram-positive bacteria and archaea FGAR-AT is a complex of three proteins: PurS, PurL, and PurQ. PurL itself contains two tandem N- and C-terminal domains (four domains altogether). The N-terminal domains bind ATP and are related to the ATP-binding domains of HypE, ThiL, SelD and PurM.
Statistics
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PSSM-Id: 100035
View PSSM: cd02204
Aligned: 149 rows
Threshold Bit Score: 148.067
Threshold Setting Gi: 49259562
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP bindingdimerization
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1T3T; Salmonella FGAR-AT with bound ATP, 3.5 A
    View structure with Cn3D
  • Comment:The N- and C-terminal repeats of PurL bind ATP/ADP

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    #   #                                
1T3T_A        687 DCAVTTasl----dsyYGEAMSIGERAPVALLDFAASARLAVGEALTNIAATQIGdikrIKLSANWMAAAGhp-geDAGL 761
gi 154496429  678 QAMAALlpvgpghetdTCSVMAWGFDPDRMARDPFGGAQASVVESLARLVASGCDyrkaYFSFQEYFEKLRtdperWGKP 757
gi 169825056 1088 QAMVAKiptmd-gimkTVSMMSYGFDADLSVQSQYLGGYYAVVESISKLIALGSDknliRLTFQEYYEKMLdk-kiWSKP 1165
gi 19704325   687 DVSIMKfpvln-kntnTASAITWGFNPYISEWSTYHGAIYAVVESLAKLVAAGVDyktaRLSFQEYFEKLGkdsykWSKP 765
gi 169334742  680 DGMVSKvplek-gdttTATVMTNGFDPELLSENPYIGAVYAVLSSVSKAVSMGADykklRLSFQEYFERLRkdptrWAKP 758
gi 167752776  675 QVSVQKlptd--gytdTASIMAFGYNPFLASWSPYHGAAYAVVDAAAKVVAAGARydkmRYSYQEYFERMTksprtWGKP 752
gi 24378558   681 EASVQKlpvqn-gvttTASVMAQGFNPYIAEWSPYHGAAYAVIEATARLVATGADwsraRFSYQEYFERMDkqadrFGQP 759
gi 38234679   669 LASVQTfpvp--ggshTASVMTYGFSPELADVSPYLMGSYSVVEALSRLVATGANargaWLSVQEYFQRLDqkpelWGEV 746
gi 160914854  671 EGSVQKlpvl--gftnTCSIMTHGYHPELSQYSPYLGASYSVVEALARVAALGGNyatcRLTNQEYFERLHndpekWGKP 748
gi 160947602  678 QAGIQAlptih-stsdTATILSYGFIPKISHYSPFLSAIYAVLESVAKVYAVGGDksnlYFSFQEYFEKLGkdskkWGKV 756
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             ###                                                         
1T3T_A        762 YDAVKAVGeELCPQLGLtIPVGKDSMSMKtrwqegneqremtSPLSLVISAFARVEDvrhtltpqlsteDNALLLIDlgk 841
gi 154496429  758 FQALLGAL-RAQLELGVaAIGGKDSMSGSfld--------ldVPPTLVSFAIAPAKAgevisp-efkagGHPVYLFQsvs 827
gi 169825056 1166 LKALLGAF-TVSKYFKAmPIGGKDSMSGNfed--------itVPPTMISFAVTHEDVdniit--ndlkgKGKIGLVRtpy 1234
gi 19704325   766 FLALLGAM-KVQKDFDVaAIGGKDSMSGTfnd--------isVPPTLISFAVSTVNVtdvist-efkkaKNKIYLVEnki 835
gi 169334742  759 FIALLGAL-KVQLELNTpAIGGKDSMSGSfed--------idVPPTLISFAVGVVDAnkvvss-dfknaGNNVYVIKidk 828
gi 167752776  753 LGALLGAL-KMQVELGLpSIGGKDSMSGTfeq--------inVPPMLMAFGITTVDAgrvist-dfkkaGHSIYLVRhtp 822
gi 24378558   760 VAALLGSI-EAQIQLGLpSIGGKDSMSGTfee--------ltVPPTLVAFGVTTVDSrqvlspefktvgENIYYIPGqai 830
gi 38234679   747 TQALLGLL-EAQDAFEVaAIGGKDSMSGTygd-------dlhVPATLVTFAVATMDArrtlsaaipsgeFDVYHLAHtpl 818
gi 160914854  749 MQALLGLI-EAELAFETpAIGGKDSMSGTfnd--------itVPPTLITFAVTTEKTqniiss-efkkaGSRIYLLKhtp 818
gi 160947602  757 TQSLLGTI-YAQNEIGCpSIGGKDSMSGTfnd--------lnVIETLISFACTPVKIknvisp-elktiGNYIYYVPivk 826
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1T3T_A        842 ghnalgatalaqvyrqlgdkpadvrdvAQLKGFYDAMQALVaarkllAWHDRSdggllvTLAEMAFaghCGVQVDIaalg 921
gi 154496429  828 y-----------------------ndyGSLKKTWENFHELCqagkvcSAWAVGsgglaeGIMKMSFgnrVGFTAEPsaes 884
gi 169825056 1235 eed-------------------gtlnlEKLEENFDFITEQIrnkniiSAIALDkkgllaNIFEQSVg-nTGFNIKFdnl- 1293
gi 19704325   836 nek------------------dflfdsQELKENFHFILKNIkdkkivSAMVVKigglaeVLSKMSFgnrLGFEINNknvd 897
gi 169334742  829 ten-------------------ntpnyDSVRSAFDLVHKLIeegkvkSSKSITvggvaeAVSKMCFgnkIGFEFLDsdyd 889
gi 167752776  823 len-------------------hmpdtGALKRNFDFVSSAIesgkivSGYAVGfggvaeALAKMSFgngIGADVEVdeat 883
gi 24378558   831 se---------------------dinfDYIKANFAQFEAIQakhkvtAASAIKyggvleSLALMSFgnhIGASVELteld 889
gi 38234679   819 et--------------------geanyEQLRANFDAFHELAatgtlsAASPIIpgglgaTIVNMLLgneLGFQGTAtt-- 876
gi 160914854  819 ken-------------------mtpnyEQLKENFAKMYQLAcehkilSASTVKfggiseALCKATFgnkLGVDVHTsepl 879
gi 160947602  827 nsk-------------------gypeiKETLKSYEQINELIksgkilSAYVQEegsvvsSLVKMAVgngLGFTVNKeni- 886
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*
Feature 1                                                      
1T3T_A        922 ddhlaalfneELGGVIQVRAedrdaveallaqygladcVHYLGQA 966
gi 154496429  885 s----lfytgMCGAILAECDgpv-------------pgGMLVGHT 912
gi 169825056 1294 -------ynpMYGSFVVEYIddd-------------enIEHIGEF 1318
gi 19704325   898 ------lfslKPASILIETTeels-----------yknAIYLGEV 925
gi 169334742  890 ------iyakNYGDIIIETSedln-----------dknAVLLGKT 917
gi 167752776  884 ------lfdnSYGSIVVESTepld-----------fpaAELLGHT 911
gi 24378558   890 s-----sltaQLGGFVFTSDeai-------------adAVKIGQT 916
gi 38234679   877 --------naALGGLVFAVPagtav---------eladAQLIGHT 904
gi 160914854  880 -------ydlSLGSILVESEteiq-----------daaFVEIGRV 906
gi 160947602  887 -------lnfDPASIVVESNce--------------lpFEKIGMV 910

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