1VPA,1W55,1I52,2PX7,1W77,1VGW


Conserved Protein Domain Family
CDP-ME_synthetase

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cd02516: CDP-ME_synthetase 
Click on image for an interactive view with Cn3D
CDP-ME synthetase is involved in mevalonate-independent isoprenoid production.
4-diphosphocytidyl-2-methyl-D-erythritol synthase (CDP-ME), also called 2C-methyl-d-erythritol 4-phosphate cytidylyltransferase catalyzes the third step in the alternative (non-mevalonate) pathway of Isopentenyl diphosphate (IPP) biosynthesis: the formation of 4-diphosphocytidyl-2C-methyl-D-erythritol from CTP and 2C-methyl-D-erythritol 4-phosphate. This mevalonate independent pathway that utilizes pyruvate and glyceraldehydes 3-phosphate as starting materials for production of IPP occurs in a variety of bacteria, archaea and plant cells, but is absent in mammals. Thus, CDP-ME synthetase is an attractive targets for the structure-based design of selective antibacterial, herbicidal and antimalarial drugs.
Statistics
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PSSM-Id: 133009
View PSSM: cd02516
Aligned: 140 rows
Threshold Bit Score: 177.33
Threshold Setting Gi: 31541291
Created: 28-Oct-2011
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding site
Evidence:
  • Structure:1vpa_a; Thermotoga Maritima CDP-Me_synthetase bound to CTP and Mg++
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             # ######            #                                                      
1VPA_A        14 NVAILLAAGKGERMSen------vPKQFLEIEGRMLFEYPLSTFLkseaidGVVIVTRrewfevvekrvfhekvlGIVEG 87
gi 31541291   10 TVGIILASGSSSRIGlt-----dqLKQFYLVNNKMVYEYSLDQFLasnlfdLIYLVVNeqhlemikakhsdnhiiKVIVG 84
gi 7531238    36 TSVVLLAAGESRRFSqt------iKKQWLRSNHTPLWLSVYESFKealdfkEIILVVSeldyiyi---krhypeiKLVKG 106
gi 166215425   4 LTLVLLGAGNSSRFGld------vKKQWLWCGNEPLWLYVTNRFTdmfpfhDVVITAHpkeipfy----kkfchhTIVKG 73
gi 166973734   4 ITLILLGAGNSTRFEsd------vKKQWLYSGEIPLWLHVAESFEamgafaKIVIVSSpediglm----khfadyTYVQG 73
1W55_A         4 MSLIMLAAGNSTRFNtk------vKKQFLRLGNDPLWLYATKNLSsfypfkKIVVTSSnitymk-----kftknyEFIEG 72
gi 166973730   4 VTLIVLCAGNSTRFEhk------tKKQWIRVEDEPLWLNVTKRISsfakfdKIIVTSHqdelnym---knfsddfTFVKG 74
gi 123549967  17 ITLILLAAGSSSRFEld------iKKQWLRVGDRPLWHFVADRCEktgyfdKIIITSSlddiefm----knyadyTFVEG 86
gi 51701569    6 VSLIMMAAGDSTRFCasnaqtfrcKKQWLRVGEEPLWLVATRNLThhfsfkQVILTASskdyvym----qnispyKVVQG 81
gi 51701552    4 VSLVVMGAGESSRFRqgl----siKKQWLRLGETPLWKHVALHLSkqacfdQVIITASpkevkym----qkqvdfEVIEG 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ## ##  #                      ###                                               
1VPA_A        88 GD-TRSQSVRSALeflek---fspsYVLVHDSARPFLRKKHVSEVLrra--retGAATLALKNSDalvrve--ndrieyI 159
gi 31541291   85 SKiNRHLSFIHAIndliacgyddqtKIVVHDSARPNINQTILSDCLnal--dhyQCVSLYQDIASslikidqinqsqehL 162
gi 7531238   107 GA-SRQESVRNALkii------dsaYTLTSDVARGLANIEALKNLFltlqqtshYCIAPYLPCYDtaiy------yneaL 173
gi 166215425  74 AD-TRQQSLLNALemv------ktpYVLVSDIARPCIQKEVVQKIIdnr--skaACIVPYIQPADtvvy------knetI 138
gi 166973734  74 GE-TRQASLKNALedv------tteYVLVSDIARCCVPAAMIHRILdar--eeaSCIVPVLPVTDtlyl------aqtpL 138
1W55_A        73 GD-TRAESLKKALeli------dseFVMVSDVARVLVSKNLFDRLIenl--dkaDCITPALKVADttlf------dneaL 137
gi 166973730  75 GE-TRQLSILNSLqfv------ttkYVMITDVARACVPQSVIENLLnek--ssaDCIVPILNVTDtvvy------endtI 139
gi 123549967  87 GQ-TRQQSLKNSLlhv------dteYVLVSDVARSCIDEAFLKKIIshv--gksDAVVPYLNITDtivy------ddktI 151
gi 51701569   82 GQ-TRCQSLRNALkyv------dtpLVLVSDVARWDSSDSIIKTMLkald-esvACVVPFVGVADtsfy------egeyL 147
gi 51701552   76 GG-TRQESLQNALasv------ktpWVLVSDVARFDVPSEVISRVIgrl--geaECVAPAIGVSDtvsy------qgeyL 140
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                                                                 #          
1VPA_A       160 PRKGVYRILTPQAFSYEILKKAHEngg---ewADDTEPVQKlg-vKIALVEGDPLCFKVTFKEDLELA 223
gi 31541291  163 DRDQIKMIYTPQATWLKTVKDLVDdqqnhldlTDFLLKLKGv---KAHWIKTKIDCFKITTLADLEHF 227
gi 7531238   174 DREAIKLIQTPQLSHTKALQSALNqg----dfKDESSAILQafpdRVSYIEGSKDLHKLTTSGDLKHF 237
gi 166215425 139 DRNEVKLIQTPQLSSTDILRKALTqgk---efTDESSAIQAlg-eKVFYILGNRKQHKLTYKEDMKLL 202
gi 166973734 139 DREQVRIIQTPQLSVTKTLKQALQtdq---lfTDDSSAVASmg-kKVHFVEGSHEAHKLTTISDLKKL 202
1W55_A       138 QREKIKLIQTPQISKTKLLKKALDqnl---efTDDSTAIAAmg-gKIWFVEGEENARKLTFKEDLKKL 201
gi 166973730 140 DRSNVKLIQTPQLSSTQVLRDALNtpi---efTDESSAIKAid-gKIKYIQGSTFSKKLTLGDELDEL 203
gi 123549967 152 DRERVKRVQTPQLSLTTALKSALLree---efTDESSAIVAng-gSREFILGQESAHKITHIEDLKKL 215
gi 51701569  148 KRESIKLIQTPQLSRVEDLQEALKdts--kdfSDESSALYAlg-kKIAFVQGSELMNKLTFSSDLKAH 212
gi 51701552  141 SRSEVKLIQTPQLSHVESLLLAFRqg----dfTDESSAIAKsg-gRVLLVEGSERLKKLTHPHELTLL 203

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