Conserved Protein Domain Family
Glucosylceramide_synthase

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cd02520: Glucosylceramide_synthase 
Glucosylceramide synthase catalyzes the first glycosylation step of glycosphingolipid synthesis.
UDP-glucose:N-acylsphingosine D-glucosyltransferase (glucosylceramide synthase or ceramide glucosyltransferase) catalyzes the first glycosylation step of glycosphingolipid synthesis. Its product, glucosylceramide, serves as the core of more than 300 glycosphingolipids (GSL). GSLs are a group of membrane components that have the lipid portion embedded in the outer plasma membrane leaflet and the sugar chains extended to the outer environment. Several lines of evidence suggest the importance of GSLs in various cellular processes such as differentiation, adhesion, proliferation, and cell-cell recognition. In pathogenic fungus Cryptococcus neoformans, glucosylceramide serves as an antigen that elicits an antibody response in patients and it is essential for fungal growth in host extracellular environment.
Statistics
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PSSM-Id: 133012
View PSSM: cd02520
Aligned: 26 rows
Threshold Bit Score: 205.524
Threshold Setting Gi: 7635885
Created: 7-Mar-2005
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
ligand binding
Feature 1:ligand binding site
Evidence:
  • Comment:Predicted ligand binding site based on similar proteins.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               # #                                                                      
gi 54647606   51 PGVSLLKPLKGVDSNLINNLETFFELDYPk-fEILLCVQDLDDPAVDVCKKLLGKYPsVDAKLFIGgkkvginPKINNLM 129
gi 116625503  21 PPLSILKPVHGRDPQFYKAILSHATQEYPe-fEILFGTNNVEDPALPDIRRLQKEFPnRRIEIVIAnnd-apnAKVGVLE 98
gi 22297608   45 PAVSILVPVCGLEARAWQNWSSLCEQNYPv-yEVLFGVQSPNDPAIPVLQAICETYP-DRARWYLChpirginLKASNVS 122
gi 23012853   50 PGVTILKPLCGLEPDLFENLESFCRQDYAgavQVVFGVQNAADPAIAVVERLRAAHPaLRLDLVVDasqhgsnRKVSNLI 129
gi 158338881  62 PPVTILKPLCGVDVNTYENLASFCHQDYAe-yQVIFSVREATDPCLPVVEQLILAFPdVDIELVVCdrtigtnLKVSNLA 140
gi 53725302   44 EPVSVLKPLCGSEPHLYENLATFCEQRHPr-yQLLFGVASAADPAIAVVRRLQADYPdCDIELVIDarvygsnLKVSNLV 122
gi 67154001   75 RPVSMLKPLHGAEPRLYENLRDFCRQTHPd-yQLIFGVREADDHAIAVVHRLCAEFPhLDIDLVIDprvhganLKVSNLL 153
gi 124515866  58 PSILMIKPVKGLDEGARENFLSFLQQDYPe-yQILFVVGDGSDPVVELLRELQAEYP-EKVRFKIIfehsgtnRKMNNVN 135
gi 94967755   61 PPVTLLKPVHGTEPQLKQNLESFFQQDYPd-fEIVFGARSLDNDAVRTVNELRKKYAhVKSSLIISgepewhnAKVYSLD 139
gi 91203103   41 EGISFLKPITGEVYNLYNNIKSFLDLRAIp-iEILFGVSSKDDPAYGILTKLENEFPgICKIILCSnhkkysnEKVGKLI 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        #                                                               
gi 54647606  130 PGYEVAKydlIWICDSGIKVKPDTLtdmanq--mtekvGLVHGLpyvadrqgfaatleqvyf--gtshprsyisanvtgi 205
gi 116625503  99 ELAKLARfpvLLVNDSDIVVEPGYLhavtap-lanpgvGLVTCLyraaaqswparsealgia--tefapsvmvarllgva 175
gi 22297608  123 QLFAHARydvVVETDSDVRVRSNYLatltqp-ladpqvGVVTCGyidhqpqrlgaafvalgrcldfipsvlvarrldggl 201
gi 23012853  130 NMSENIAhevVVLADSDMSVRPDYLerivaa-lsqpgvSGVTCLyhgvpgnrgvfaqlaalaidvqfvpnvilgtsfdla 208
gi 158338881 141 NGVEKAKhdvIVLADCDILVQSDYLqqivqp-lanpevGVVTCLynswakgwlagfealgit--trnhanvlaahklegm 217
gi 53725302  123 NLAERARhgrIVIADSDIAVEPDYLtrvtap-ladpsvGVVTCLyharsvggfwtrigaqfv-dawfapsvrithlggss 200
gi 67154001  154 NMLPLARhdwLVLADSDISVPADYLvrvtap-ladpgvGIVTCLyygvpqesfwsrlgalfi-ddwfapsvrlshvfgst 231
gi 124515866 136 RAFEGEKgdlVLLNDSDIRVDPKYLksivrpmlddpsvGMVTCLqrgtpaggwssrlaslml-nteaipqalvayrlfpi 214
gi 94967755  140 KMIQSTPnshFIITDSDIVVEHDFIrriipp-lndpkvGCVTAMykgvpapefwsrmealgm-svempsgvmvvdmlegm 217
gi 91203103  120 TLTEHARydiINISDADVLVPQDFLqslth----iknaGMVTNLyrgihnegigghleviti-lsdifqgvcmakifhgi 194
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                                                   
gi 54647606  206 kCVTGMSCLMRKEVLDQAGgliaFAQYIAEDYfmAKAIAdrgwkfsMATQVAMQNsgcysisqFQSRMIRWAKLR 280
gi 116625503 176 dFALGSTMVFRTEALERIGgfraIANYLADDYqlGRHIAqlgyrieFAPVVVETDlgggswgqIWRHQLRWSRTI 250
gi 22297608  202 rFAIGPTVLLRREVLEKIGgfeiALNRIGEDYqlGYAAWragyrveLSTYILDNDcgndsllsAVQRELRWSRSI 276
gi 23012853  209 rPCFGSTIAMTAESLARIGgfraFKDDLADDYaiGEALRaeggtvaIPTFTIGHAcvdtelagLWRHELRWNRTI 283
gi 158338881 218 hFAFGSTIVIRQAALAKIGgfaaLADHLADDFhmGNLPTqvgyqvvLSPYIVEHQlattnlseFFHRQARWARCI 292
gi 53725302  201 rFGFGATLALTRATLDAIGgfkaLKDELADDYwlAELPRrlglrtvLSEVNVATDvaepsfapLWLRETRWLRTI 275
gi 67154001  232 rFAFGSTIALRREVLQAIGgfevLRDTLADDFwlGELTRraglrtvLSDLLVGTEvsetrlieLWTHELRWLRTI 306
gi 124515866 215 dFAFGPTMLLRRDALEKSGgfsaLTDILADDYhlAQNLVragykihLSPYLVDARigeespgdLWRHEIRWVRTY 289
gi 94967755  218 kFALGAVMAVRRDSLKSIGgiqaTREFYSDDFvlGNLISeagyevvLSDVKVGHVltsrsfakTFGDQLRWMQST 292
gi 91203103  195 nYGFGASMFLTKQSLESIGgyeaLGNMLADDYhlGNKISkngnpvtLSTILVNTVvgksnfknYFVRQLRLCKTY 269

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