1JYL


Conserved Protein Domain Family
PC_cytidylyltransferase

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cd02523: PC_cytidylyltransferase 
Click on image for an interactive view with Cn3D
Phosphocholine cytidylyltransferases catalyze the synthesis of CDP-choline.
This family contains proteins similar to prokaryotic phosphocholine (P-cho) cytidylyltransferases. Phosphocholine (PC) cytidylyltransferases catalyze the transfer of a cytidine monophosphate from CTP to phosphocholine to form CDP-choline. PC is the most abundant phospholipid in eukaryotic membranes and it is also important in prokaryotic membranes. For pathogenic prokaryotes, the cell surface PC facilitates the interaction with host surface and induces attachment and invasion. In addition cell wall PC serves as scaffold for a group of choline-binding proteins that are secreted from the cells. Phosphocholine (PC) cytidylyltransferase is a key enzyme in the prokaryotic choline metabolism pathway. It has been hypothesized to consist of a choline transport system, a choline kinase, CTP:phosphocholine cytidylyltransferase, and a choline phosphotransferase that transfers P-Cho from CDP-Cho to either lipoteichoic acid or lipopolysaccharide.
Statistics
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PSSM-Id: 133014
View PSSM: cd02523
Aligned: 55 rows
Threshold Bit Score: 155.083
Threshold Setting Gi: 91792522
Created: 8-Mar-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitemetal binding
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site
Evidence:
  • Structure:1jyl_a; CTP:Phosphocholine Cytidylyltransferse binds to CDP-choline.Mg+, defined by 3.5 A distance.
    View structure with Cn3D
  • Citation:PMID 9370319
  • Comment:Multivalent coordination of Mg2+ drives the conformation change of the enzyme which is pivotal to the reaction.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           ####                                                                        #
1JYL_A        28 AIILAAGLGTRLRPLTentPKALVQVNQKPLIEYQIEFLKekgiNDIIIIVGylKEQFDYLKek-ygVRLVFNDkyadyN 106
gi 76883134  312 AIVLGATRGKGLESLTqdrPKVMLPVAGIPLLRRLVDEFKrqhvNRITVVTGykPEAIDVKA-----ISLIHNPdyghsS 386
gi 94310746  307 AVVLAAGRGKGLEALTedkPKIMLPVAGKPLLRWLVDGFKnqgiNDITVVGGykAEAIDAAG-----IKLVRNErhaatG 381
gi 37719596    3 AIILAAGFGSRLMPLTkdqPKCMVEYKNKKIIDYEIEALKsagiNEIAVVGGylNDVLKNYLnkydiEHFFINSkydktN 82
gi 76801622    2 AVHLAAGQGTRLRPLTddrPKPLVELGGQSLLEQNVETLKeagvRDHVVVTGyeGDQIVDRG-----FETVHNDvydetE 76
gi 162455587 297 AVILAAGQSQGLLPLTeelPKPMLDLKGKSILERQIDVLNacgvKDVAVVRGykKEAIKVPN-----ARFYDNDeyattN 371
gi 161528170   4 LIILGAGEGTRLRPLTqncPKCMVNIFGKSLLEHQINTAKncgiDEIIIVKGflGYMIQIPN-----IRYYENKyydstN 78
gi 121997566   3 AIILAAGQGTRLRPLTddkPKCLVELAGKSLLDHQLDVLRaagvDDIHVVAGyrADQLERPG-----FTRHINEryaetN 77
gi 117618315   3 AIILAAGRGSRLGSMTenqPKPLAKLAGKSLIEWQLASLAaagvDSVHVVCGycSEALQGYG-----DSRIMNTnwassN 77
gi 24982125    3 AVILAAGRGSRLQALTderPKALVPFNGVPLLQRAIETLRrcdvSEVGIVAGyrSEMLAPFA-----DTLFYNHrwhssG 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #  #                      ##                                      #             
1JYL_A       107 NFYSLYLVKeel-------anSYVIDADNYLFknMFRNDltRSTYFSVYred----------ctnEWFLVygdd------ 163
gi 76883134  387 ELSSLACALesl------sedTIITYGDLLFRsyILNDLrhVPGNIVVVvdsal-------spgmKDDIRdvafcsqpdd 453
gi 94310746  382 ELASLACAAdsl------etdTVVSYGDLMFRsyILRDLleTDAPFSVVvdssesapsnqsvrdfAWCSAaddrdlfgq- 454
gi 37719596   83 MVHTFFCAKdfmlkcieekqdLIISYADIVYFqdCVQKLinAKEELAIVvdks---------wrkLWSKRfanpledaet 153
gi 76801622   77 MIYSLFCAAeafpd--sgegdLLISYGDIVYErdVVEALldCSAPLCVAvdrq---------wrkLWEERfedplsdaet 145
gi 162455587 372 EVVSLFRASkel------sgpFLFLYGDIVFErgHLEKLlkSPADISIVidr----------ayaEGQRPkdargvpdlv 435
gi 161528170  79 MVETLFCAEkel------nesVIVSYGDIIYQknILKKLidSPDDFSVVidkn---------wknYWQIRfsnilddves 143
gi 121997566  78 MVGTLFAAEsvmt----gesdLIISYGDIVYEprVLAALqaVDAPIGLAvdre---------wrrYWAARmddpladaet 144
gi 117618315  78 MVRSLMCADavls-----aapTLVCYGDIVYRpgIIRTLisSDAPLAISydke---------wwsLWSARfedplsdaes 143
gi 24982125   78 IFSSLNCASdwls-----aepCLVSYGDIFYDstLVSELinHPADIVLGydpa---------avrLWQQRfndplpdlen 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                      # 
1JYL_A       164 ---------------------ykvQDIIVDSkagRILSGVSFWDaptAEKIVSFIDKAyvsg-----------efvdLYW 211
gi 76883134  454 rsl---------frqdvdllrietQKKQLSKqphGRWIGMARVAgegRQWLLDALQALrqqe-----------nfsqLAM 513
gi 94310746  455 ---------------kvllrkisdTPAATDGephGRWIGLLNVRgegRARLQKLLAELrqrp-----------dfdsLDM 508
gi 37719596  154 l------------kmtngyiielgKKANAYDeieAQYIGLFKFSyqfLSEVIAFYEMLdrdilyd------nknfenMYM 215
gi 76801622  146 ld-----------iddkgriheigETPEGYDtidAQYVGLLKVRndhIDRFVDAYTELdaqtd----------gyvsIDT 204
gi 162455587 436 alkdaqeagyrfvgaeaphrvarvGRAIPAGeahGEFVGMAMFTakgARLLTDCYHQLqesrakggf--heaeslrnATF 513
gi 161528170 144 l------------rlkdgyimdigQKPNSLDnidGQYIGLMKFQhnaLSTLKTFYHNSkeqsklgtnplnpkipftkSYM 211
gi 121997566 145 lk-----------ltdgdrvvelgKKPEGYAdiqGQYTGLIKVRadhVRRLPEVWRAMdrhatyd------gkdydnMYM 207
gi 117618315 144 f------------rqqggqlltigEQVQTREeieGQYMGLLKFTpegWQRVSAVLAGLsaeq------------inkLDM 199
gi 24982125  144 f------------rlvdgwvsaigQTATSLEviqGQYMGLFKVTpagWQALKKQLQGLtpqa------------rdqTDM 199
                        250       260       270
                 ....*....|....*....|....*....|....*..
Feature 1        #                        # #         
1JYL_A       212 DNMVKDNikeldVYVEELeg-nSIYEIDSVQDYRKLE 247
gi 76883134  514 PDLFNHLva-ngHPIKVLyingHWLDVNDMEDLTRAG 549
gi 94310746  509 PDLINALve-agEKVEVRyvhgHWRGVNDLEDFRRAG 544
gi 37719596  216 TSFLQALie-kyNNAKAVeidgNWCEIDFMSDLEVQI 251
gi 76801622  205 TAFLQQLid-dgWHLQAVsidrGWLEVDTLEDLERYR 240
gi 162455587 514 TDLLQELid-rgADVQAIdvykGWLEIDTFEDYRRAW 549
gi 161528170 212 TDLLQGLih-ngQNIKAIeingGWLELDTLNDFEIYQ 247
gi 121997566 208 TSFIQHLid-mgWEARAAfidnGWAEVDCEADLAAAT 243
gi 117618315 200 TSLLRLLls-hgVPIASIaisgGWVEVDNPSDITLYE 235
gi 24982125  200 TSLLGTLvs-egFPVAGVathaPWGEIDCPSDVALYE 235

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