1LVW,1H5R,1MC3


Conserved Protein Domain Family
G1P_TT_short

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cd02538: G1P_TT_short 
Click on image for an interactive view with Cn3D
G1P_TT_short is the short form of glucose-1-phosphate thymidylyltransferase.
This family is the short form of glucose-1-phosphate thymidylyltransferase. Glucose-1-phosphate thymidylyltransferase catalyses the formation of dTDP-glucose, from dTTP and glucose 1-phosphate. It is the first enzyme in the biosynthesis of dTDP-L-rhamnose, a cell wall constituent and a feedback inhibitor of the enzyme.There are two forms of Glucose-1-phosphate thymidylyltransferase in bacteria and archeae; short form and long form. The homotetrameric, feedback inhibited short form is found in numerous bacterial species that produce dTDP-L-rhamnose. The long form, which has an extra 50 amino acids c-terminal, is found in many species for which it serves as a sugar-activating enzyme for antibiotic biosynthesis and or other, unknown pathways, and in which dTDP-L-rhamnose is not necessarily produced.
Statistics
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PSSM-Id: 133019
View PSSM: cd02538
Aligned: 29 rows
Threshold Bit Score: 383.464
Threshold Setting Gi: 168700509
Created: 14-Nov-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
substratetetramer
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding site
Evidence:
  • Structure:1h5r_a; E. coli Glucose-1-phosphate thymidylyltransferase binds with dTMP and glucose-1-phosphate
    View structure with Cn3D
  • Structure:1mc3_a; Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             # ##                                                                       
1LVW_A         4 MKGIVLAGGSGTRLYPITRAVSKQLLPIYDKPMIYYPLSVLMLAGIRDILIISTprdLPLYRDLLGDGsqf-gvrFSYRV 82
1H5R_A         4 RKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTLMLAGIRDILIISTpqdTPRFQQLLGDGsqw-glnLQYKV 82
gi 159882064   1 MKGIILAGGSGTRLYPITKGINKHLLHIYDKPMIYYALSVLMLAKIKDILIITNpeyVENFENLLGNGshi-ginISYAS 79
gi 134046342   1 MKGIILAGGSGTRLYPMTYAGNKHLMPLFNKPMIYYSLSVLMLSKIKEVLIISTpeaLPAYEKLFGNGdql-ginISYAG 79
gi 15718522    1 MKGIILAGGSGTRLYPSTLTLCKQLLPIYDKPMIYYPMSVLMLAKIKEVLIISTpkdIDRFKELFKDGsfl-gmqIQYKI 79
gi 116625281   1 MKGVVLAGGTGSRLFPLTKITNKHLLPIYDRPMIYYPIQTLVDAGIQEILIVTGgrnSGDFLRLLANGkqfglkhINYTY 80
gi 730818      1 MKGVILAGGNGSRLMPLTKAVNKHLLPVGPYPMIYWSIMKLQEAGIKDILLISQkehMPQFYKLLGNGeel-gvtITYQV 79
gi 19916115    1 MKGVILAGGTGSRLYPLTKVTNKHLLPIYDKPMIYYPMETLINAGIKDIMIVSGrghAGHFLELLGSGvdf-gvhFTYEI 79
gi 206900850   6 VKGVILAGGTGSRLRPLTKVTNKHLLPVGRYPMIFYPIYKLKQAGIYEILVVTGrehMGDVIELLGSGrdf-gveFTYKV 84
gi 168700509   3 IRGVILAGGKGTRMGELTRVTNKHLLPVGAWPMVYHPLKKLTGVGLQEILLVSGtehMGDFVELLGSGkdh-gcrLTYRV 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #  ####                     #                                      #            
1LVW_A        83 QEEPRGIADAFIVGKDFigdskVALVLGDNVFYGhrFSEILRRAasl---edGAVIFGYYVRDPRPFGVVEFDseg-rvI 158
1H5R_A        83 QPSPDGLAQAFIIGEEFiggddCALVLGDNIFYGhdLPKLMEAAvnk---esGATVFAYHVNDPERYGVVEFDkng-taI 158
gi 159882064  80 QEKPKGIAEAFIIGEKFirdsnVALILGDNLFYGqgFSDILVKAfqn---keGATIFAYPVKDPERFGIAEFDenf-nvI 155
gi 134046342  80 QAEPKGLAEAFIIGEEFigddsVCLILGDNIVYGsgLTGILEKSyenienngGAIVFGQYVNDPHRYGVLEFDknk-kvT 158
gi 15718522   80 QERPEGLAQGLILAQDFiqnddIALILGDNIFYGqgFSDILENAkddl-kkgFASIFSYHVKDPERFGVVEMDqng-nvL 157
gi 116625281  81 QEGEGGIADALALAEHFadghqICVVLGDNIIEGn-IKAAADRFraq---paGAHILLKEVQDAERFGVAEVAgd--riV 154
gi 730818     80 QPAASGISDGLSYAKRFtkkesFILLLGDNIFEDs-LKPYTERFeqq---gkGAKVLLKEVDDPERFGIAEIDeknkriR 155
gi 19916115   80 QEKAGGIAQALSLAEDFvdgdsVTVILGDNIFQDn-IKEDVANFn------sGAKIFLKEVTDAHRFGVAELKgk--kvI 150
gi 206900850  85 QDEAGGIAQALGLARHFvgddkCVVILGDNIFEDd-ITPYVKKFeeq---keGAKILLKEVPDPQRFGVPVFEng--riI 158
gi 168700509  82 QDEAGGIAQALGLAEHFcmgsrSLVLLGDNIFRDp-LIGLLEKAntr---pdWAWIGLKEVHDPGRYGVAELDgd--kvL 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           ##         #                           #                                     
1LVW_A       159 SIEEKPSRPKSNYVVPGLYFYDNQVVEIARriEPSDRGELEITSVNEEYLRmgkLRVELMgrgmAWLDTGTHDGLLEASS 238
1H5R_A       159 SLEEKPLEPKSNYAVTGLYFYDNDVVQMAKnlKPSARGELEITDINRIYLEqgrLSVAMMgrgyAWLDTGTHQSLIEASN 238
gi 159882064 156 SLEEKPKKPKSNWAVTGLYFYDNEVVDIAKkiNPSERGELEITSINSEYLKrnkLKVQLLgrgfAWFDAGTHESLLEASL 235
gi 134046342 159 KVIEKPENPPSNYAVIGLYYYDNDVINISKsiKPSGRGELEITDVNNVYLDknkLDVELFprgiAWFDAGTHESFLDASN 238
gi 15718522  158 SLEEKPKNPKSNYVATGLYFYNNDAIDIAKniKPSARGELEITDVNIEYLRlnkLKSQHLgrgfAWIDTGTHDSLIEASS 237
gi 116625281 155 GIEEKPNNPKSNYAVTGIYMYDASVFQKIKtlVPSGRGELEITDVNNAYIRegnMSFSYLd--gWWTDAGTFDSLLRATN 232
gi 730818    156 SIIEKPEQPPTNLCVTGIYMYDAEVFSYIEqiSPSKRGELEITDVNNLYIEnsqLTYDVLs--gWWVDAGTHESLYLASQ 233
gi 19916115  151 GIEEKPKNPKSDFAVTGLYIYDSDVFNAIKtlKPSGRGELEITDVNNYYINkgmMEYGILd--gFWSDAGTFESLLRAGM 228
gi 206900850 159 AIEEKPKEPKSNFAVTGIYMYDSYVFEIIKtlKPSARGELEITDVNNTYLKmgkLTYDILk--gWWTDAGTPESLFRANE 236
gi 168700509 156 GIEEKPEKPKSPMAVVGIYIYPPDVFGLIKtlKPSRRGELEITDVNNHYIQqgrMGCFNLd--gYWTDAGTLDSLDYANE 233

                 ....*
Feature 1             
1LVW_A       239 FIETI 243
1H5R_A       239 FIATI 243
gi 159882064 236 FVSTI 240
gi 134046342 239 YIHAI 243
gi 15718522  238 FVQTI 242
gi 116625281 233 LVAQS 237
gi 730818    234 LVHQA 238
gi 19916115  229 LVKQN 233
gi 206900850 237 LARNI 241
gi 168700509 234 LVRKE 238

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