2B0C,1CQZ,1S8O,2I6X,5AHX,3CNH,4JB3


Conserved Protein Domain Family
HAD_sEH-N_like

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cd02603: HAD_sEH-N_like 
Click on image for an interactive view with Cn3D
N-terminal lipase phosphatase domain of human soluble epoxide hydrolase, Escherichia coli YihX/HAD4 alpha-D-glucose 1-phosphate phosphatase, and related domains, may be inactive
This family includes the N-terminal phosphatase domain of human soluble epoxide hydrolase (sEH). sEH is a bifunctional enzyme with two distinct enzyme activities, the C-terminal domain has epoxide hydrolysis activity and the N-terminal domain (Ntermphos), which belongs to this family, has lipid phosphatase activity. The latter prefers mono-phosphate esters, and lysophosphatidic acids (LPAs) are the best natural substrates found to date. In addition this family includes Gallus gallus sEH and Xenopus sEH which appears to lack phosphatase activity, and Escherichia coli YihX/HAD4 which selectively hydrolyzes alpha-Glucose-1-P, phosphatase, has significant phosphatase activity against pyridoxal phosphate, and has low beta phosphoglucomutase activity. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319790
Aligned: 46 rows
Threshold Bit Score: 117.446
Created: 3-Feb-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Escherichia coli soluble epoxide hydrolase phosphatase domain and on other haloacid dehalogenase superfamily members with structure
  • Structure:2B0C: Escherichia coli soluble epoxide hydrolase phosphatase domain binds glucose-1-phosphate, contacts at 4A
    View structure with Cn3D
  • Structure:3CNH: Deinococcus radiodurans predicted haloacid dehalogenase-like hydrolase binds phosphate ion and Na+ in the active site
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              #####                                                                     
2B0C_A         7 KMLYIFDLGNVIVDidf-------------nrVLGAWSdltriplas-lkksfhmgeAFHQHERGEISd----------- 61  Escherichia col...
YP_144878      1 MRGALLDRDGVLLLldekalyrkavelaargaGLERSLaalaravralneavrglavRTLEEEEALWRtl---------- 70  Thermus thermop...
NP_353821      4 IDHIVFDIGKVLIHydphipy----srlipdaDERQWFfenv-----------cthdWNLEQDRGRRWedaealllerfp 68  Agrobacterium f...
NP_295067      7 LQAVLFDRDDTLALtdpavy------reaalwMQERFGldprqa-------ghtlaqVWEERMNDWWDlrsh-------e 66  Deinococcus rad...
NP_857051      3 ISAVVFDRDGVLTSfdwt----------raeeDVRRITglp--------------leEIERRWGGWLNgltiddafvetq 58  Mycobacterium b...
NP_771464      9 ADALLFDLGRVVLDidf-------------skAIACWAghagckpeaiiaryvrdseAYRLHEVGKISd----------- 64  Bradyrhizobium ...
NP_767998     69 IKLVLFDMDNVLCNydr-------------gqRVACLAelagstsel-vhkaiwdsgFELLGDSGALDa----------- 123 Bradyrhizobium ...
NP_599372      4 TRTFLFDLYGVLIKehg-------------aaQFERVAravgepskn-dklhevyesLRLDLDAGRVSe----------- 58  Corynebacterium...
YP_012189      9 ARAVLYDFSGVLAEegf-------------isGLRAIAaeng-----------ldpqAFVRAATDVCYtsgyad---gla 61  Desulfovibrio v...
YP_003771167   1 MNWIVFDYGDVLSKps---------------aALPGLAaam--------------gaPLPEFLEAYWAyripyd---ags 48  Amycolatopsis m...
Feature 1                                                                         ##             
2B0C_A        62 --EAFAEALCHemalp------lsyeQFSHGWqavf---valRPEVIAIMHKLRe--QGHRVVVLSNTnrlh--ttfWPE 126 Escherichia col...
YP_144878     71 -vLEVARELRVppe----------elLPWRYYrfm-----rpAPGAERLLHRLKa--RNLKVGVLSNTlpslreslaHHG 132 Thermus thermop...
NP_353821     69 erEEHIRAFRK---------------FWHEMVsh-------sYDDSVAIMVALId--SGHDVTMLTNFasdt--freAQK 122 Agrobacterium f...
NP_295067     67 deEQFWEEYGSdltarlglgpeaaaeVMAAYPyery---mkpVAGAREVLSELRr--RGLKTGVLSNTlpsi-drtlDAL 140 Deinococcus rad...
NP_857051     59 piSEFLSSLARelelgsk--ardelvRLDYMAfa------qgYPDARPALEEARr--RGLKVGVLTNNsllv--sarSLL 126 Mycobacterium b...
NP_771464     65 --EEYFASLRAslg----------igISDAQFlegw--naifAGEMPDVAELLPraaKQMPLYAFSNTnrph--vdhFSK 128 Bradyrhizobium ...
NP_767998    124 --PDYLRGFGErmgyp------lsldEWVEARrrs----mqpDHAMLEIARRLR---KTVDIAVLTNNttlv--adhIDT 186 Bradyrhizobium ...
NP_599372     59 --VNYWNQIKLlvgl--------eflDIQEVIaadyrglyerDQDMVDYVLSLKa--KGHRIGILSNIpegl--aklLKE 124 Corynebacterium...
YP_012189     62 paSAFWQGVRQvtgit------hddaTLQEAVlsr----fviRPDMFADVDALRe--QGLTVALVSDHt-------dWLE 122 Desulfovibrio v...
YP_003771167  49 tpLEYWQAVGNavgap------vdeaLSAELTrvdvegwghlEPSSAALLEALSe--AGANLALLSNApia---fgeWVR 117 Amycolatopsis m...
Feature 1                                #                       ##  ##                          
2B0C_A       127 EYPei---rdAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSDTVFFDDNADNIEGANQLGITSILVKDktt----ipD 199 Escherichia col...
YP_144878    133 LAR-------YVDGFFASCALGVAKPDPRAFLLALEGLGLAPEETLYLDDDPENVEAARRLGLRAEVYAPi--------G 197 Thermus thermop...
NP_353821    123 MFPfl----tLPRGVTVSGDVKLLKPDVAIYHLHAKEFGLNPATSLFIDDTLANVEGAKAAGWQAVHFTGa-------eK 191 Agrobacterium f...
NP_295067    141 GLAd------LIDVPLATCLLGVHKPEARAFTLAAEALGCRPEEVLFIDDRPENVSAAQAVGMRAALIDHsgqt-pgalS 213 Deinococcus rad...
NP_857051    127 QCAal---hdLVDVVLSSQMIGAAKPDPRAYQAIAEALGVSTTSCLFFDDIADWVEGARCAGMRAYLVDRsgqtrdgvvR 203 Mycobacterium b...
NP_771464    129 EYAdl---lgHFRELYLSSSIGLRKPDAAAFDHVVAAIGVPARRIVFFDDLAENVEGARARGLTAVHVTSprd----vgD 201 Bradyrhizobium ...
NP_767998    187 LLPdlr--plFGSRIYTSAQFKTAKPDPRCYRRCLAELDVMPQTVLFVDDLPANVAGAREAGLFAHHHTSv-------eA 257 Bradyrhizobium ...
NP_599372    125 HNSew---ldQLDAVTLSCDIGAAKPEPKSFHVALEALGEKAEDVTFIDDRVRNIEAAREEGLSTIHFTGl--------D 193 Corynebacterium...
YP_012189    123 TLDdrhgvfhHFDHAFSSFREKRNKRTGELFDRALDVLGLAPEQTMFFDDNAGNVERAILRGIDARLFTDk-------pA 195 Desulfovibrio v...
YP_003771167 118 AQDwa----rYFRVTMFSGDVRCVKPEAKIFRLLLDELGAEPADCLFFDDRESNVEGARAVGLQAHVWNGa--------D 185 Amycolatopsis m...
Feature 1              
2B0C_A       200 YFAKVL 205 Escherichia coli K12
YP_144878    198 APGRRA 203 Thermus thermophilus HB8
NP_353821    192 LKQDLR 197 Agrobacterium fabrum str. C58
NP_295067    214 DLREVL 219 Deinococcus radiodurans R1
NP_857051    204 DLSSLG 209 Mycobacterium bovis AF2122/97
NP_771464    202 ALEALG 207 Bradyrhizobium japonicum USDA 110
NP_767998    258 FRQALS 263 Bradyrhizobium diazoefficiens USDA 110
NP_599372    194 SLKESI 199 Corynebacterium glutamicum ATCC 13032
YP_012189    196 YRADLA 201 Desulfovibrio vulgaris str. Hildenborough
YP_003771167 186 AARAWL 191 Amycolatopsis mediterranei U32

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