1S2O,2B1Q,2D2V,1U2S,2B1R,3GYG


Conserved Protein Domain Family
HAD_SPP

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cd02605: HAD_SPP 
Click on image for an interactive view with Cn3D
sucrose-phosphatase, similar to Synechocystis sp PCC 6803 SPP
Sucrose-phosphatase (SPP; EC 3.1.3.24) catalyzes the dephosphorylation of sucrose-6(F)-phosphate (Suc6P)-the final step in the pathway of sucrose biosynthesis in plants and cyanobacteria. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319792
View PSSM: cd02605
Aligned: 20 rows
Threshold Bit Score: 241.099
Threshold Setting Gi: 169629697
Created: 31-Aug-2004
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Synechocystis sp. sucrose-phosphatase and other haloacid dehalogenase (HAD) superfamily members with structure
  • Comment:sucrose-phosphatase is inhibited by sucrose and other disaccharides
  • Structure:2D2V: Synechocystis sp. sucrose-phosphatase binds inhibitor maltose in the active site, contacts at 4A
    View structure with Cn3D
  • Structure:2B1R: Synechocystis sp sucrose-phosphatase binds inhibitor cellobiose in the active site, contacts at 4A
    View structure with Cn3D
  • Structure:1U2S: Synechocystis sp. sucrose-phosphatase binds inhibitor glucosein the active site, contacts at 4A
    View structure with Cn3D
  • Structure:2B1Q: Synechocystis sp. sucrose-phosphatase binds inhibitor trehalosein the active site, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####                                   ###                         #       
1S2O_A         5 LLISDLDNTWVgdq------qalEHLQEYLGDRrg--nfYLAYATGRsyHSARELQKqv--glMEPDYWLTAVGSEIYHp 74
2D2V_A         5 LLISDLDNTWVgdq------qalEHLQEYLGDRrg--nfYLAYATGRsyHSARELQKqv--glMEPDYWLTAVGSEIYHp 74
3GYG_A        24 IVFCDFDETYFphtideqkqqdiYELEDYLEQKskdgelIIGWVTGSsiESILDKXGrgk-frYFPHFIASDLGTEITYf 102
gi 116000505  38 LIVSDLDGTMVgnd------eytRAFRQFWESSakppgsKLVYSTGRslESFTRLIEakadvlVQPDGLICAVGTKVFQr 111
gi 32473570   24 VLATDLDGTFLpldgd---daavASMKQIREQLeai-dvPLVFVTGRhfDSVRDAIGef--nlPKPQWILCDVGVSIYKa 97
gi 15888002    6 LLSTDLDGTVVgdn------datRRFRDFWHALpddlrpVLVFNSGRliDDQLALLEev--plPQPDYIIGGVGTMLHAk 77
gi 81650592    5 LLLFDFDETYFkhntneedlshlREMEKLLEKLtnnnevITVVLTGStfQSVMDKMDqvn-mtFKPLHIFSDLSSKMFTw 83
gi 123593429   6 LLCSDLDRTLLpnghqaespqarLRLQRLAQRPg----iILAYVSGRhkALIQSAIRey--dlPLPDFAIGDVGTTIYQi 79
gi 81693986    5 LLLFDFDETYFkhntneedlshlREMEKLLEKLtnnnevITAVLTGStfQSVMDKMDqvn-mtFKPLHIFSDLSSKMFTw 83
gi 81844176    5 LILFDFDETYYkhrtnqadipylKEMKSLLQNItaknnvITAILTGStiESVLQKMSkvn-msYKPQHIFSDLSSKMFTw 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                         # #            
1S2O_A        75 e--------glDQHWADYlse-------hwqRDILQAIADgfealkpq---------spleqnpwKISYHLdpq------ 124
2D2V_A        75 e--------glDQHWADYlse-------hwqRDILQAIADgfealkpq---------spleqnpwKISYHLdpq------ 124
3GYG_A       103 sehn---fgqqDNKWNSRin----------eGFSKEKVEKlvkqlhenhnil-lnpqtqlgksryKHNFYYqeqdein-- 166
gi 116000505 112 asddastawteDVQWTAAldvnws--celvtRAAQEAIDAc-----------------------gHENAHFqpadeqnrh 166
gi 32473570   98 dgeg---gyirESAYEQI-------------LDEVLGDIDiqkhrlaigelenftlqesykqgrhKWSYYVpadql---- 157
gi 15888002   78 kr------selETAYTQSlg----------tGFDPRKIADvmnriagvtm------qeeryqhglKSSWFLhda------ 129
gi 81650592   84 nnge---yvesETYKKKVls----------ePFLFEDIEDilhhisaqynve-fipqrafegnetHYNFYFhstgn---- 145
gi 123593429  80 tdn----qwhpWEDWSKEisqdwqginqaglAKLFADITPlrlqep-------------ekqnryKLSYYAppel----- 137
gi 81693986   84 nnge---yvesETYKKKVls----------ePFLFEDIEDilrhisaqynve-fipqrafegnetHYNFYFhstgn---- 145
gi 81844176   84 nnce---yiesDEYKNEVli----------ePFLLEDILDilkhvsskhkve-fipqrifrenetLYNFYFyssgd---- 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #           # #                             ##
1S2O_A       125 -----acpTVIDQLTEMLKetg-----ipvQVIFSSg--------kDVDLLPqrSNKGNATQYLQqhlamepsQTLVCGD 186
2D2V_A       125 -----acpTVIDQLTEMLKetg-----ipvQVIFSSg--------kDVDLLPqrSNKGNATQYLQqhlamepsQTLVCGD 186
3GYG_A       167 --dkknllAIEKICEEYGVs---------vNINRCNplagdpedsyDVDFIPigTGKNEIVTFXLekynlnteRAIAFGD 235
gi 116000505 167 kvtigvrdEIVSEVETMIRqacdsngldytVIVSGSgg------wkFVDCVSagAGKLESLEYVRkrlgfellDTVACGD 240
gi 32473570  158 ---eachqAVEAYLKLHELp---------cSIVSSIdpfn---ndgLVDVLPhgASKAFALHWWCdqhelqpeQIVFCGD 222
gi 15888002  130 ------daAALGEIEAALLaad-----idaRIVYSSd--------rDLDILPkaADKGAALAWLCgqlrigldESVVSGD 190
gi 81650592  146 ---hnndrRILEVLVRYANdqn-----ytaRFSRSNplagdpenayDIDFTPsnAGKLYATQFLMkkynipvkSILGFGD 217
gi 123593429 138 -----dweNLIPQLAQRLQaqg-----iqaSFIWSVdeta---qigLLDILPkrANKLHAIRFLMerqhfdksHTVFAGD 204
gi 81693986  146 ---hnndsRILEALVRYANdqn-----ytaRFSRSNplagdpenayDIDFTPsnAGKLYATQFLMrkynipvkSILGFGD 217
gi 81844176  146 ---thldkTILEDLSQYSKtrd-----ytmTFNRCNplagdpenayDINFTPknAGKLYATKILMnkygvpkeLIIGFGD 217
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1        #  ##                                                                     
1S2O_A       187 S-GNDIGLFETs-aRGVIVRNAq-------PELLHWYdqwgdsrh-----------yraqsshAGAILEAIAHF 240
2D2V_A       187 S-GNDIGLFETs-aRGVIVRNAq-------PELLHWYdqwgdsrh-----------yraqsshAGAILEAIAHF 240
3GYG_A       236 S-GNDVRXLQTv-gNGYLLKNAt-------QEAKNLHnlit------------------dseySKGITNTLKKL 282
gi 116000505 241 S-GNDILMLSGq-tRCIIVGNAe-------SELRDWAsecvangelsl-----ervflaseneALGILQGLAKF 300
gi 32473570  223 S-GNDTAALIAg-yRSVVVGNAdrkiarkvADAHQAAgwvdrlk-------------largtsTSGVLEGARWF 281
gi 15888002  191 T-GNDRAMFELktiRGVIVGNAl-------PELVSLAhqdnrff-------------hstakeADGVIEGLRHW 243
gi 81650592  218 S-GNDEAYLSYl-eHAYLMSNSr-------DEALKQKfrlt------------------kypyYQGITLHVKEF 264
gi 123593429 205 S-GNDLEVLASg-lQAILVRNAq-------EEVRQEAlrrlppehsqqlylarggfmglngyySAGVLEGLAHF 269
gi 81693986  218 SgNDEAYLSYLe--HAYLMSNSr-------DEALKQKfrlt------------------kypyYQGITLHVKEF 264
gi 81844176  218 S-GNDEAFLSYl-dHAMIMSNSq-------DEEMKSKfknt------------------kypyYKGIYTHVREF 264

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