2YY6,3D6J


Conserved Protein Domain Family
HAD_PPase

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cd02616: HAD_PPase 
Click on image for an interactive view with Cn3D
pyrophosphatase similar to Bacillus subtilis PpaX
This family includes Bacillus subtilis PpaX which hydrolyzes pyrophosphate formed during serine-46-phosphorylated HPr (P-Ser-HPr) dephosphorylation by the bifunctional enzyme HPr kinase/phosphorylase. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319797
View PSSM: cd02616
Aligned: 14 rows
Threshold Bit Score: 249.889
Threshold Setting Gi: 56707633
Created: 19-Aug-2004
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #####                                 #                                   
2YY6_A         1 XRVILFDLDGTLIDSaKDIALALEKTLkelgleeyypdNVTKYIGgGVRALLEKVlkdkf----reeyVEVFRKHYlenp 76
3D6J_A         6 YTVYLFDFDYTLADSsRGIVTCFRSVLerhgytgitddXIKRTIGkTLEESFSILtgitda-dqlesfRQEYSKEAdiyx 84
gi 169637561   3 VRAVLFDLDGTLIDTiPLIRWTFERVFadfgl-pwengEVLHTVGlPLREIAARYmp---------drADEFMERYaafq 72
gi 51316531    3 INTVLFDLDGTLINTnELIISSFLHTFetyypgkygrkDAIECIGpPLTDSFKRLdper-----veemVATYRKHNhahh 77
gi 51316500    2 IKAVLFDLDGTLINTnDLILKSFKHTFktmldlepseeEITMNYGrPLQEIFKSYde---------nrIEEMINCYrkin 72
gi 51316489    3 ITAVLFDLDGTIIDTnQLIIKSFVYTVekhlgykigaeEVIPYFGePLPLTLQRFsk---------dkWEIMLKTYrdyn 73
gi 226704848   3 INTVLFDLDGTLINTnELIISSFLHTLnhyysnqykreDVLPFIGpSLHDTFSKIda---------skVEEMITCYrqfn 73
gi 83590837    2 FAGVLFDFDGTLVDTsELVIKSFQHTLapylgrvvapkEVYPYFGvTLKEGLAAFmpdh-----ldemLHEYRRYSaahf 76
gi 51316467    3 IRTILFDLDGTLIDTnTLIKASFEHTFkeynl-nfsneEILKFNGpPLVDTFNKIdetk-----adrmITTFREHNireh 76
gi 206890000   3 IELIIFDLDGTLVDScKDITQALNYCFkkrgiegfsqeEVKKMVGeGVNRLIEKAlqlrklslpvqdlVECFINYYkkhi 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       ###                              #               
2YY6_A        77 ----vvyTKPYPEIPYTLEALKSkgfKLAVVSNKleELSKKILDIlnlSGYFDLIVGGDTFGEKKPSPTPVLKTLEILGE 152
3D6J_A        85 ----nanTILFPDTLPTLTHLKKqgiRIGIISTKyrFRILSFLRNhxpDDWFDIIIGGEDVTHHKPDPEGLLLAIDRLKA 160
gi 169637561  73 ktrfrelTRAYPGAVETLATIKSagyRTGVVTSKrrEPALASLALtglDQHIEAVVTADDVTKPKPDPEPVFKALELLHT 152
gi 51316531   78 ----dklVEPYEGVYETVKTLHEqgfKLAIVTTKirETAMKGLKLfglDEFFDVIVALDDVENVKPNPEPLEKAMNALGA 153
gi 51316500   73 lelhddeCKEFADVDLMLKTLKSkgiKIGVVTSKksDMAERGAKLmgiFKYFDTFITPEITTKHKPDGEPVLKACENLGV 152
gi 51316489   74 ekyhdryTKIREDVKEVLARLKEegiKTAVVTSKrrELAKRGLKLfelDKYFDVLVGLEDTEKHKPEPDPVLKALELLKS 153
gi 226704848  74 hehhdelVEEYETVYETVQELKKqgyKIGIVTTKarQTVEMGLKLsklDQFFDVVVTIDDVEHVKPHPEPLQKALELLDA 153
gi 83590837   77 ----ddlVRPCPGVREGLQRLQQagiKLGVVTSRlrDTTLYGLELcglTSFFPVIVAAEDVTSHKPGPEPVRYGLELLGV 152
gi 51316467   77 ----dnfVTAFPHVYDTLEELQNrniSLGIVSTKmrHTVHMGLELtgiSKFFSTIITYDDVTHAKPHPEPVQMAMQKLGA 152
gi 206890000  83 ----adfSIVYPNVRETLEKLQGi--KKAVISNKltELSIKTLETlglLEYFDFIAGSDLFAERKPSPVPIIETIKRFNT 156
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                ##  ##                                          
2YY6_A       153 EPEKALIVGDTDADIEAGKRAGtKTALALWGYvkl---nsqIPDFTLSRPSDLVKL 205
3D6J_A       161 CPEEVLYIGDSTVDAGTAAAAGvSFTGVTSGXttaqefqayPYDRIISTLGQLISV 216
gi 169637561 153 RPQNAAYIGDSWYDVVAGKQAGvTTVGATWGIasreqlaehAPDIIVDSWDEFLAN 208
gi 51316531  154 KKEETIMVGDNSHDILGGKNAGvKTAVVGYAIrgedyvrqfDPDYVLRSMPDLLDI 209
gi 51316500  153 SPSEALMVGDSPYDILAGKNAGaKTCGVKYTAlpleklgesNPDFYVDKPLEILDL 208
gi 51316489  154 PREEALMVGDSPYDILSARSAGvRSVAVKWSVlpfellkkeKPDYFIEDMWQLLKI 209
gi 226704848 154 KPEETLMVGDNHHDIVGGQNAGtKTVAVSWTLkgrayleayKPDYVLDKMSDLLPI 209
gi 83590837  153 EAGAAAMIGDSPHDIQAARAAGvTSVAAGWSRvprdqilaaGPEVLVASMTEFVDF 208
gi 51316467  153 HPEHTLMVGDNHHDIVSGQRANvQTAAVAWSLkdtnylksfHPDYIIEDIKDIITI 208
gi 206890000 157 SPDKTIIVGDSELDIKAGTLAGvKTVAVTYGYreka--llkNADFIIDKFSDLITI 210

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