Conserved Protein Domain Family
GH18_CFLE_spore_hydrolase

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cd02874: GH18_CFLE_spore_hydrolase 
Click on image for an interactive view with Cn3D
Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
Statistics
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PSSM-Id: 119353
View PSSM: cd02874
Aligned: 31 rows
Threshold Bit Score: 314.203
Threshold Setting Gi: 24214908
Created: 21-Jul-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #                             ##                                           
3CZ8_A         7 IAGTLSFYVLRnpdl---drelINDYAPYSSSISIFEYHIApngdi--------anqlNDAAAIETTWqr-------rVT 68
gi 51892928  132 GPVVVAYYAEDwegdv-ralnaLRRAGARVDRVVNFALELHadgs----------vttRSYPALAAAAae------lgVP 194
gi 89098445  258 LTWEAVYSENPdh--------aALPSMPGVNVVSPTWFELAgedgs--------vknlGSGDYVKWAHgr-------gYQ 314
gi 125975376 256 LAWDMIYTRREdh--------sSLGEMKGLDVISPTWFQVKnakge--------linrAYSKYVDWAHsr-------gYQ 312
gi 34482818   23 GLERLVYMVDRsds-----lslLQERIDSIEVVAPQVFSFTlegt---------vygeVDPRIIALAKer-------gKK 81
gi 134100449  59 VVASLPFWNLGngt------atVVQNRTAVNEVSPWMYGLGddgr---------ltiqYSPAQATEVAghlqrlreaqIP 123
gi 51891616   73 GFWIAGYYVDYdsds----lesVRLNAHHLDQVIVFGYGFDaegna-------vgkdqTLIRGVTSHQk--------rVL 133
gi 116619936  17 QPKALFYLNDSpas-----irsFQANADKIDVIVPTVYSVDaagl---------vwgkPDPRVLETSRkf-------rVA 75
gi 24214908  117 ADENTSFRASTwfsd----yeaMKKTVHLYNEIHPFIYGMKgretnngdlysswgssqKHERISELRKln------pnIK 186
gi 148288678 169 VVAVDGFYTLEegetlpssyesFVTNINLISEICLFMFRIDpsdataie-kfgdftdeYVKELVNIAHrn-------nVR 240
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        #                                  # # #                        
3CZ8_A        69 PLATITNLts----ggFSTEIVHQVLNNPTARTNLVNNIYDLVSTRGYGGVTIDFEQVSAADRDLFTGFLRQLRDRLq-a 143
gi 51892928  195 VYGLVHNMqn----gqFSAEIARAVLSDPARRSRAVADMLAIAREHGLAGIDIDIENVPGDLRAGYTALAAEAAAVLk-p 269
gi 89098445  315 VWGLFSNA--------FDAELTHQTFKDFETRQKIIRSLLHYSQIYKLDGINLDIENVNQEDGPLVTQFVREATPYFhqa 386
gi 125975376 313 VWALLSNDf-------TDSEMTSKFLNNTDARDNLIREILAYAALYNLDGINIDFENMYISDRDVFTQFVREIAPLLreq 385
gi 34482818   82 LMPLVMNEn-------FEKEKIKAFLANPKAQERLIGSLIKIALKEGFGGWQLDFEHLGITSKEAYTQLVRQLYARLkeh 154
gi 134100449 124 IVPSLANIte----gnWSYEPVARMLHDPELMRRHVFEIVELVKRENYAGIDIDYENLRAGDREQFTRFVTDLGRALhae 199
gi 51891616  134 LFGNFSDAg-------FDPDLAHRILTDPVVQERAMSSMLDEAGRLGVSGIQIDFENIPPEDREAYTQFLRRLKERLepl 206
gi 116619936  76 LMPIIVNPg-------FKQDMIHALMANAEARRRMNATLLAECRKYGYYGIQFDFENINYLDRDLLASMVKETSAALgrg 148
gi 24214908  187 IIPTIFRWen----kyEKISENIGMNGRNDIRDKHLSNILYELDTYGYDGIDIDYEGMSCEKKEKFEEFIVILSKEIhkr 262
gi 148288678 241 ALAVVHNLlyrpggttKAKDLVKSLVSTRENRQIFINNLISLIEKYNFDGVNIDIEDVYIEDKDNLSSLYLEMGREFr-r 319
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 # ##                   
3CZ8_A       144 GGYVLTIAVPaktsdn--------------------ipwlrGYDYGGIGAVVNYMFIMAYDWHHa---gSEPGPVAPITE 200
gi 51892928  270 GGIRLTLSVPaktwddt------------------tsnwggAFDYAALGEIADEIAIMAYDEHTw---gLPHGPVASLPW 328
gi 89098445  387 GLTVSMDITFisssg----------------------nwsaFYERDKLAGIVDYLAVMAYDEHWat--sPVAGSVASLPW 442
gi 125975376 386 GLVVSVDVNDi-----------------------------qCYDKKALSEAVDYIMYMSYDQHWst--sPVAGSVAQVSW 434
gi 34482818  155 HLSLSIAVVPprgeepknay-----------gakvfedwvsPYDLKALAEHSDFLTLMAYDQHTg---gSTPGPIASRAW 220
gi 134100449 200 GKTLSVAVFAktsdag-------------------ydernvAQDYAAIGQVADQVRLMGYDFHWg---tSRPGPVAPVGW 257
gi 51891616  207 GLTLSVAAAAktsdtt--------------------sgwggATDYAAIGKIVDHFYIMAYDQHWl---gGEPGPVASLDW 263
gi 116619936 149 GFKLSIATVPnegdepgksdy----------trwvfqnwrgAFDLRQLAPYVDFISLMTYDQHTr---nTPPGPVAGMTW 215
gi 24214908  263 GKILSVAVHPktpakkesmkackglkdkikmdyaenwrgpmTHDYAFLAKYADRIKIMAYELHPrkyrnPGPGPQAPNTW 342
gi 148288678 320 KGYFLSASIPsrvsdep------------------fnpfsdPFDYRIIGSAVDEFIVMLYNEHGwp--gSGPGPVVSIGW 379
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 #                                                      
3CZ8_A       201 IRRTIEFti--aqVPSRKIIIGVPLYGYDWIipy----qpgTVASAIsnqNAIERAMRy-------------------qA 255
gi 51892928  329 VERVVAYav--tqIPPEKILLGIPAYGYDWIag------trQVVGGLstgGAYSLAARh-------------------gA 381
gi 89098445  443 VESNLQAll--evVPKEKLILGVPLYARLWEekd----eggISSEALsmeQVKEWLTEt-------------------gA 497
gi 125975376 435 QEKIVKRvleqegVPREKLLLGIPFYTRLWKetvdesgkkkLTSSALtmkQAKNLIIEn-------------------nA 495
gi 34482818  221 VEGIIQEal--iwVPKEKLSLGIPLYSRLWHagike-ekmrGVAKSLtfaGAMDWLERq-------------------gA 278
gi 134100449 258 IRDVLSYat--svVPRERIVLGVPLYGYDWVdg-------qGTSVTWl--QAFKLATEh-------------------kV 307
gi 51891616  264 TERVIRYai--gvMPSQKILLGVPLYGYEWAlep----elaDTNAAYgpgRMQRRAAQf-------------------gA 318
gi 116619936 216 VEKLAAYae--sqMPKAKISLGVALYGRRWSagmrd-kdpaVAASTIyaaDAVDLAKAi-------------------dA 273
gi 24214908  343 IKSIIEYak--arVPAKQLYMAIPTYGYDWAlnc----nskIKSVYY---QDAVRKKEfgihrqptnidqilantknssS 413
gi 148288678 380 MNRVLNYti--trVPRNKVVAAVSVFGFDFNlt-------tGRNTYVtyaGAIEIAKRy-------------------gK 431
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                                                                 #             
3CZ8_A       256 PIQYSae-yqSPFFRYSDq------qGRTHEVWFEgVRSMSRKMQIVREYrLQAIGAWQLTLAEGHHHHHH 319
gi 51892928  382 EVRWDde-aqVPWFRYVAd-------GAEHIVYFEnSRSAAAKLRLVGRYgLGGIAIWRLGLNEDGLWEVV 444
gi 89098445  498 VPVYDeasgqNYAEYYSVk------dKATYKIWLEdEMSLRKRAEIADRHgLAGVGSWSRYFADQSAWLAL 562
gi 125975376 496 KVEWDee--sGQFYAEYTk------dNTNYRLWLEdANSINLRTSLVHKYrLAGTCAWSIYFVSEDIWDVL 558
gi 34482818  279 ETLWLer--dGVKWAMGEr------gGVKEYLFMEeARSLALKVELAKRYgLRGISLWRLGQEDPRIWDEL 341
gi 134100449 308 PTFYDar-tqSPWFAYTDa------qGRRHEVWFEnTASSKAKFEAARGSgIRGVYLWMYGYEDTGTWPEL 371
gi 51891616  319 EVVWDpvhaeNRAVFLTD--------EGQRVAWFPdERSLEAKLRLAYQYnLKGIAVWRVGLEPDDWWHRM 381
gi 116619936 274 TVQWDpl--eHAPWFYFYr------dGIREYVFYNdARSFEDRYAMARERgLHGISAWILGSEDPGLWRSL 336
gi 24214908  414 WINLSkfswvHTGKTYEDpsiwykseGCDRVAFYMnRKAFEDKMNLLRQYdLGGFSFWQLISDNDPGINDY 484
gi 148288678 432 DIIFDee-tkTPMFSYVDe------nGNNHEVWFEnAESIYAKAELAFNKgIKGIALWRLGMEDNKIWDSM 495

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