Conserved Protein Domain Family
GH18_chitobiase

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cd02875: GH18_chitobiase 
Chitobiase (also known as di-N-acetylchitobiase) is a lysosomal glycosidase that hydrolyzes the reducing-end N-acetylglucosamine from the chitobiose core of oligosaccharides during the ordered degradation of asparagine-linked glycoproteins in eukaryotes. Chitobiase can only do so if the asparagine that joins the oligosaccharide to protein is previously removed by a glycosylasparaginase. Chitobiase is therefore the final step in the lysosomal degradation of the protein/carbohydrate linkage component of asparagine-linked glycoproteins. The catalytic domain of chitobiase is an eight-stranded alpha/beta barrel fold similar to that of other family 18 glycosyl hydrolases such as hevamine and chitotriosidase.
Statistics
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PSSM-Id: 119354
View PSSM: cd02875
Aligned: 8 rows
Threshold Bit Score: 511.978
Threshold Setting Gi: 66819083
Created: 1-Aug-2005
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative active
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #                                 #              
gi 399376     21 GLALLALLALLALrlaa----gtdCPCPEPELCRPIRHHPDFEVFVFDVGq-------KTWKSYDWSQITTVATF----G 85
gi 50751384    9 LVLLLGLLQLLGRta--------aCPCQDPRLCQPIAGTDRFEVFVFDVGk-------EAWKSYDWSKITTVAAF----G 69
gi 66819083    3 VIFLLILLILLKIvks------yeCNCILKEYCKPLEVGERIEFIGFSFNs------tGNYLNYDYYSVTTIAISyqsnY 70
gi 66818433    5 IIISLILTILIIQsks------keCPCSNVELCKPLEIGPRKEFIGFSVNs-------TFYPYYNWDHLTTIGIFy--sE 69
gi 66821553    2 RIILLLFLIVFVVaqssssssssgCPCDSSYLCEPLQIAPRQEFLGFSLNs-------TQYPNYYWNQLTTLAIFy---E 71
gi 32450115    7 GYWALSPLFIMLIcvsl---csagCPCTDPSLCEPINSTRDSEVLVFYTRg-------ENWKFYDWSQVTTIALF----G 72
gi 66472280    5 LSILVLTFCVQVWsk--------vCPCERKELCQPISSQPAFEVFVFDVGe-------KAWKFYDWTKVTTIAAF----G 65
gi 156368735   6 RWLAVICVFSFAIsltl----crkCPCKEESLCEPITRPPGKECLVWTTTpnvwrktdFNAPGYDWSKITTIGMF----R 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                             # # #                      
gi 399376     86 KYDSELMCYAHSKGARVVLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVnclSPEYDALTALVKETTD 165
gi 50751384   70 KYDPELLCFAHSKGARVVLKGDVPLKEVVDPAKRAAWISQQVDLAKKQYMDGINIDIEQEVnetSPEYYALTELVKETTD 149
gi 66819083   71 DIPEELVCKSHENRVRLVWSAIISNNQISNETYRNQFIDSIVETIQKENMDGVIYELDNPNdltIENSDYFTEIIKKTYQ 150
gi 66818433   70 DIEDELLCMAKTNGVRLVYSAFYPVEQLSNETYIDQWINEKLELVQNTFTDGLNFDVEYPIt-dPIVAQQYTQLVAATNN 148
gi 66821553   72 TIEDELLCIAHENDVRLVWGTTFPIENLGNSSYIEEWIQEQIEKVQSTFTDGLNFDVESPIt-dPTIAQQYTELVSATNK 150
gi 32450115   73 KYDPQLLCFAHLKGARLVLKGDVPLQDIVDPNNRTSWIAQKVELAKSQFMDGINLDIEQSVlkgSPEYYGLTALVQETTE 152
gi 66472280   66 QYDAELMCYAHSKGVRLVLKGDIRLPEIVDPVNRTAWIQGNVKLAKSQFMDGINIDIEQAVetgSPEYYALTDLVKETTE 145
gi 156368735  78 EWDDELFCTAHSKGVRVVLVSGFPVEKLSNSQVRKDWIKNKTTEAKENFADGINIDIEGPIekgSKEVTLLTKLVNNVAK 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    ##                                  
gi 399376    166 SFHREIEGSQVTFDVAWSPk-NIDRRcYNYTGIADACDFLFVMSYDEQSQIws---eCIAAANAPYNQTLTGYNDYIKMS 241
gi 50751384  150 AFHKEIPGSQVTFDVAWSPa-CIDKRcYNYTGIAAACDFLFVMSYDEQSQIwt---dCIAKANAPYLQTLFGYEEYITMG 225
gi 66819083  151 KLKLINWNYQISAKFPFSP--NIDFS-YNYPEISYYSDYLVIMDYNIGSRVsndgnyCLAGSNSPNPLIEQGVHNFTSMG 227
gi 66818433  149 AFKSVNLYYQISVDVAWDAsnCIDYRcYDYLGLSKASDFLVVMDYDMKLDGyyf-ktCLASANSPPSSVLSGMVNFTKLG 227
gi 66821553  151 AFKAINPFYQISIDVAWSPs-CIDKRcYDYAGLASNSDFLVAMDYDERSQVfge-kvCTAGANSSPSNALAGINNFTDLG 228
gi 32450115  153 AFHREIPGSQVTFDVAWCPd-CVDGRcYNYTGIAESCDFIFVMSYDEQSQIwt---eCEAGANSPLNKTISGYEKFIQLG 228
gi 66472280  146 SFHTEIPGSQVSFDVAWSPk-CIDKRcYDYITIADSCDLLFVMSYDEQSQIwg---dCIAMANAPYDQTLTAYDQYISMN 221
gi 156368735 158 AFRKEFKHTQISFDAAWSPd-CIDGRcYDLKAIVDIVDFMVIMAYDQQSQIke--gpCIAKANSPYSQTLEGVQQYLKLN 234
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                                   
gi 399376    242 INPKKLVMGVPWYGYDYt-CLNls----eDHVCTIAKVPFRGAPCSDAAGRQVPYkt-----iMKQINSSi---sGNLWD 308
gi 50751384  226 IDPKKLVMGVPWYGYDYv-CLNls----eDHVCSLPKVPFRGAPCSDAAGHQVPYge-----iMKQVNSSi---sGALWD 292
gi 66819083  228 IPGERLVLTYPWFGLDFi-CFNgn---lsSVDCLVTKSENVSCNDINSISNQISYge-----iLKILNDDniingGINWN 298
gi 66818433  228 IDESSLVMGLPWYGYNYp-CIGsny-tlqTFECIIPPSSYLGYNCTDASGIEINYsi-----iMNMLNDTaiqngGVQWD 300
gi 66821553  229 ISTDQLVMGLPWYGYIYknCLNgdeagleTVVCQIESVPFRGANCSDAAGSEYDYsylvqllqDQTINSS-----AVQWN 303
gi 32450115  229 IEPKKLVMGVPWYGYDYp-CLElf----qDNKCTIKEVPFRGAPCSDAAGRQIPYsk-----iMKQVNSSl---tGRLWD 295
gi 66472280  222 IDPKKLVMGVPWYGYDYs-CLNfs----kDGVCTIPKVPFRGAPCSDASGRQIPYsi-----mMKQINSSi---sGRLWD 288
gi 156368735 235 ISNNKLVLGLPWYGYDYp-CEEid---ekNDICFIKKVPFRGVNCSDAAGTQIPYatv--ndlLMTRTDT-----GRKWS 303
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                                                      #                        
gi 399376    309 kd-qrAPYYNYKDPAGHFHQVWYDNPQSISLKATYIQNYRLRGIGMWNANCLDYSgdavakqQTEEMWEVL 378
gi 50751384  293 ev-qnSPFYEYKDSLGHFHQVWYDDPRSISLKAAYVKNRGLKGIGMWNGNSLDYSreavaekQTEAMWQAL 362
gi 66819083  299 sq-skSVFFNYYDKQGILHQIHFDNPKSLFIKVFLTKRLKAKGIAAQSMDNLDFNdlvnsynMFDTMAYFF 368
gi 66818433  301 sesesPYFNFIDLFSGTQHQMWFDNPDSLTIKVNIARTMNLRGVGVWNIDQLWDDh-----sLSSGMWGAL 366
gi 66821553  304 tewqsPYFNYIDPITGNVDQVWFDNPQSLSIKVQLAQKLNLRGVAVWNIDFLDFSdq----yNSRPMWDAL 370
gi 32450115  296 dv-qkSPFYNYKDAKGQLHQVWYDDPVSISLKSAYVKIHGLRGIGMWNGDLLDYSrnpiaetQTKSMWNAL 365
gi 66472280  289 ee-qrAPYYNYKDTEGMVHQVWYDDPESIALKAAYVMQHGLKGIGMWNGNLLDYNgdpiaqkQTVDMWNAL 358
gi 156368735 304 lk-slSPYFWYSDHVGNPHQVWYDDPSSLTFKYRIANMLDLKGVAIWNADLLDYSglmranvQTPDMWNKL 373

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