Conserved Protein Domain Family
PGM1

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cd03085: PGM1 
Click on image for an interactive view with Cn3D
Phosphoglucomutase 1 (PGM1) catalyzes the bidirectional interconversion of glucose-1-phosphate (G-1-P) and glucose-6-phosphate (G-6-P) via a glucose 1,6-diphosphate intermediate, an important metabolic step in prokaryotes and eukaryotes. In one direction, G-1-P produced from sucrose catabolism is converted to G-6-P, the first intermediate in glycolysis. In the other direction, conversion of G-6-P to G-1-P generates a substrate for synthesis of UDP-glucose which is required for synthesis of a variety of cellular constituents including cell wall polymers and glycoproteins. The PGM1 family also includes a non-enzymatic PGM-related protein (PGM-RP) thought to play a structural role in eukaryotes, as well as pp63/parafusin, a phosphoglycoprotein that plays an important role in calcium-regulated exocytosis in ciliated protozoans. PGM1 belongs to the alpha-D-phosphohexomutase superfamily which includes several related enzymes that catalyze a reversible intramolecular phosphoryl transfer on their sugar substrates. Other members of this superfamily include phosphoglucosamine mutase (PNGM), phosphoacetylglucosamine mutase (PAGM), the bacterial phosphomannomutase ManB, the bacterial phosphoglucosamine mutase GlmM, and the bifunctional phosphomannomutase/phosphoglucomutase (PMM/PGM). Each of these enzymes has four domains with a centrally located active site formed by four loops, one from each domain. All four domains are included in this alignment model.
Statistics
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PSSM-Id: 100087
View PSSM: cd03085
Aligned: 30 rows
Threshold Bit Score: 943.191
Threshold Setting Gi: 18655891
Created: 1-Nov-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1KFI_A; Paramecium tetraurelia parafusin (phosphoglucomutase) binds two sulfate ions and Zn in the active site cleft; defined at 4A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1KFQ_B         9 RVQVTQPYAGQKPGTSGLRKKVSEAtqpNYLENFVQSIFNTLRkde-lkPKNVLFVGGDGRYFNRQAIFSIIRLAYANDI 87
1KFI_A         9 RVQVTQPYAGQKPGTSGLRKKVSEAtqpNYLENFVQSIFNTLRkde-lkPKNVLFVGGDGRYFNRQAIFSIIRLAYANDI 87
gi 91776277    4 HIISTQPFDDQRPGTSGLRKRVSVFqqpHYLENFVQSIFDAVQv----pAGATLVVGGDGRFYNLEAIQTILKMAAANGI 79
gi 57864860    4 RTVTTTPFDDQKPGTSGLRKAVPTFqkpHYLENFIQSIFDSLDg----cEGQTLVVGGDGRYYNRQAIQVILKMAAANNI 79
gi 730308      3 KTIKTTPYQDQKPGTSGLRKKVPVFaqeNYAENFIQSIFDALEg----fEGQTLVIGGDGRYYNREVIQKAIKMAAAAGF 78
gi 151570202   4 QTVSTKPFANQKPGTSGLRNKVIAFqqpGYLENFVQSIFNSLDd----iEGKTLVVGGDGRYYNDVAIQIIVRMAAANGF 79
gi 161018469  12 KTVLTTAFEGQKPGTSGLRKKVSVFqqpHYVENFIQSLFENIGp----lEGKMLILGGDGRTFNRTLLQIVLKMAAANGV 87
gi 68223880    1 MEVTTTAYKDQKPGTSGLRKKVTVFqqpNYTANFVQSTFNALHrq--gaVPDVLVVGGDGRYYTSEAVQVILKVSAANGV 78
gi 163777876   4 QEVPTQAFDDQKPGTSGLRKKVTVFqqpNYTNNFIQATIDAVTelegslAGATLAVGGDGRFYLPEAIQIIIKMAAANKI 83
gi 55275414    4 LQIATRPFADQRPGTSGLRKKVKVFqqpHYLENFVQSIFNAIPd----rAGKTLVLGGDGRFYNREAIQIILRMAAGNGW 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               ###                                      
1KFQ_B        88 SEVHVGQAGLMSTPASSHYIRKVNeev-gncIGGIILTASHNPGGKehGDFGIKFNVRTGAPAPEDFTDQIYTHTTKIKE 166
1KFI_A        88 SEVHVGQAGLMSTPASSHYIRKVNeev-gncIGGIILTASHNPGGKehGDFGIKFNVRTGAPAPEDFTDQIYTHTTKIKE 166
gi 91776277   80 GRVLVGKNGILSTPAASCVIRKYKt------FGGIILSASHNPGGPt-GDFGIKFNSANGGPAPEKVTEEIFARSQEMTQ 152
gi 57864860   80 GRILVGCGGIFSTPAASAVIRKYNa------FGGIILSASHNPGGPn-GDFGVKYNVSNGGPAPEKVTNAIYECTKTINE 152
gi 730308     79 GKVLVGQGGILSTPAASNVIRKYKa------FGGIVLSASHNPGGPt-EDFGIKYNIGNGGPAPEKITDAIYARSKVIDS 151
gi 151570202  80 AKIIVGQNGIFSTPAVSCVIRKYEa------FGGIVLSASHNPGGPk-GDFGIKYNVSNGGPAPEKITDRIFSETKKINQ 152
gi 161018469  88 SRVKMGRGGILSTPAVSHLIRKYHa------HGGLILSASHNPGGPe-GDCGIKYNIANGGPAPNSLCDAIFATSQRLSF 160
gi 68223880   79 RCVWVGQHGLLSTPAVSTMVRRRRdadgrkaTGAFILTASHNPGGPd-ADFGIKYNSENGGPAPEKLTSQIYEETVKITH 157
gi 163777876  84 GRLVIGQNGLFSTPAVSALIRQRQl------RGGIILTASHNPGGPt-EDFGIKYNIANGGPAPGSVTDTIFKKSQALTK 156
gi 55275414   80 GKVVVGRGGLFSTPAVSTVIRARGa------HGGIILSASHNAGGPd-QDFGIKYNTANGGPAPEKLTDAIWTASRSIQG 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1KFQ_B       167 YLTvdyefekhINLDQIGVYKFegtrlekshFEVKVVDTVQDYTQLMQKLFDFDLLKGlfsnkdFSFRFDGMHGVAGPYA 246
1KFI_A       167 YLTvdyefekhINLDQIGVYKFegtrlekshFEVKVVDTVQDYTQLMQKLFDFDLLKGlfsnkdFSFRFDGMHGVAGPYA 246
gi 91776277  153 YKIvda---pdVALDIVGEIELa-------gMTVEIIDAVSDYVDLMAKLFDFEAIRQlla-sgFRLRFDAMHAVTGPYA 221
gi 57864860  153 YKIlda---adINLDRPGSFKLg-------tMDVEVIDSVTPYVELMQQLFDFDKIKSmvssdnFRMCMDSLHAVTGPYA 222
gi 730308    152 YKIsda---adIDLDKIGSFKVd-------eLTVDVIDPVADYAALMEELFDFGAIRSlia-ggFKVVVDSMSAVTGPYA 220
gi 151570202 153 YFIsdaa-kesVDLDKLGTYKIe-------nTAVEVINSVIDYAELMQQIFDFDKIRKlfa-kgFKVRFDSMCAVSGPYA 223
gi 161018469 161 YKIfea---sdVDLDRQGTTFMg-------aMQIDVIDPVVDYVALMQEIFDFDCIAKavt-rgLTFRFDAMHAVTGPYA 229
gi 68223880  158 IKMapt--lpeVDIHTLGTYTFdd-----ynFQVEVVDSLADYAAYMQEVFDFEAIRAlvqrldFKVHVDSLHGVSGPYV 230
gi 163777876 157 YQIsda---adVDLSVLGEVRVe-------dMIVEIVDPVDNYATLMQELFDFDALKAyvs-sgIKLHIDCLSGIAGPYA 225
gi 55275414  153 YQIldg---adVDIDHAATFWLg-------dMQIEVCDPVAEYAELMARIFDFDALKRlmq-gpFRMRFDALHAITGPYA 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                            # # ##      
1KFQ_B       247 KHIFGTLLGcsKESLLNCDPSEDFGGGHPDPNLTYAHDLVELLdihkkk-------dvgtvpQFGAACDGDADRNMILGr 319
1KFI_A       247 KHIFGTLLGcsKESLLNCDPSEDFGGGHPDPNLTYAHDLVELLdihkkk-------dvgtvpQFGAACDGDADRNMILGr 319
gi 91776277  222 RAILVDCLGadPDAVMNADPLPDFGGGHPDPNLTYAHDLVEIMygd-------------dapDFGAASDGDGDRNMILGk 288
gi 57864860  223 RALFEQYLGakEGTVQNGTPLEDFGGGHPDPNLVYAHDLVDIMfgd-------------napDFGAASDGDGDRNMILGk 289
gi 730308    221 VEILEKRLGapKGSVRNATPLPDFGGHHPDPNLVHAKELYDDVmsp-------------egpDFGAASDGDGDRNMVVGk 287
gi 151570202 224 KYIFETLLQapAGTVVNAQPLEDFGGFHPDPNPVNAEDLVKHMrsg--------------kyDFGAASDGDADRNMIVGk 289
gi 161018469 230 HEIFEKCLGfsEGTVVNGLPLPDFGGGHPDPNLVYAKALYDLLise-------------qgpDLGAASDGDGDRNLIIGr 296
gi 68223880  231 DRIFHEGLGvpKTSLFRTNVLPDFGGCHPDPNLTYAADLVHVMgllpdgnanpamkhistvpSFGVAFDGDADRNMILGc 310
gi 163777876 226 KRIYVDLLGapEDAVVNASPKPDFGGGHPDPNLVYAHDLVAAMqtg--------------ayDFGAAFDGDADRNMILGk 291
gi 55275414  222 HEIFEKQLGapAGTVVNGTPLTDFGGGHPDPNLVYAKPLADELfae-------------napDFGAASDGDGDRNMILGr 288
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                      #                 
1KFQ_B       320 -qFFVTPSDSLAVIAANANLif------knGLLGAARSMPTSGALDKVAAKNgIKLFETPTGWKFFGNLMDAg------- 385
1KFI_A       320 -qFFVTPSDSLAVIAANANLif------knGLLGAARSMPTSGALDKVAAKNgIKLFETPTGWKFFGNLMDAg------- 385
gi 91776277  289 -rFFVTPSDSLALIAANARLvpg----yaqGIAGVARSMPTSAAVDRVAQELdIPCFETPTGWKFFGNLMDAg------- 356
gi 57864860  290 -nFFVTPSDSLAVLTANATLvpg----yegGIAGVARSMPTSAAADRVAAKLgIDCYETPTGWKFFGNLLDAg------- 357
gi 730308    288 -gMFVTPSDSLAIIAANAKLapg----yaaGISGIARSMPTSAAADRVAEKLgLGMYETPTGWKFFGNLMDAg------- 355
gi 151570202 290 -qIDVSPSDSLAIMAANAHLipa----yskGIKGVARSMPTSTAVDRVAESLrLPCFETPTGWKFFGNLLDAe------- 357
gi 161018469 297 -qQFVSPSDSLAIMVEHAHLikg----yrkGIVGVARSMPTTPAADLVAEKHgLNFFETPTGWKFFGTLLDAg------- 364
gi 68223880  311 -rFFVNPSDSLAVLAANADCvpfftqssssGLKAVARSMPTSGAVDRVAAAHdFALFEVPTGWKFFGNLMDSkdlyggkd 389
gi 163777876 292 ngFFVTPSDSVAVLAAQYEHipyl---kknGLQGVSRSMPTGAALDLVAKKLdIEFFEVPTGWKFFGNLMDAg------- 361
gi 55275414  289 -hFFVTPSDSLAVLAAHATAipa----yrhGLKGIARSMPTSQAADVVAEALgIAHYETPTGWKFFGNLLDAg------- 356
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            #                                                           
1KFQ_B       386 -LINLCGEESFGTGSNHIREKDGIWAVLAWLTILAHknkntdhfvTVEEIVTQYWQQFGRNYYSRYDYEQVDSAGANKMM 464
1KFI_A       386 -LINLCGEESFGTGSNHIREKDGIWAVLAWLTILAHknkntdhfvTVEEIVTQYWQQFGRNYYSRYDYEQVDSAGANKMM 464
gi 91776277  357 -KVTLCGEESFGTGSDHVREKDGLWAVLFWLNILAIkr------qPVETIVKRHWARFGRNVYSRHDYEELPSDAANGLI 429
gi 57864860  358 -KATLCGEESFGTGSNHIREKDGLWAVLFWLNILAVkg------eSVEKIVRDHWQEYGRNFYSRHDYEEVESGPANELM 430
gi 730308    356 -KVTICGEESFGTGSNHVREKDGLWAVLYWLNIVAArk------eSVKDIVTKHWAEYGRNYYSRHDYEEVDSDAANTLV 428
gi 151570202 358 -KITLCGEESYGTGSNHIREKDGVWAVLFWLNLVAVtg------kQVDQLIEEHWQKFGRNFYSRHDYEAIDTAIANSIM 430
gi 161018469 365 -KVTFCGEESFGTGSHHIREKDGLWAVLFWLNLLAVtg------kTVAQIVQQHWHSYGRFYALRHDYEEVEEDKALAVL 437
gi 68223880  390 fNPLLCGEESFGTGSNHIREKDGIWASLFWLSVIAKrnapgtplvGVQQIVEEHWATYGRNYYSRYDYEDVSAEAAKAVM 469
gi 163777876 362 -RLSLCGEESFGTGSNHIREKDGVWASLCWLSVMAGtg------qSIEQILLKHWQDFGRNFFTRYDYENVSSEAGDAVM 434
gi 55275414  357 -KITFCGEESFGTGSDHIREKDGLWAVLAWLSVIAHtg------qPVAEIVTRHWQRFGRHYYTRHDYENLPAEIGEQIM 429
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 # ###       #          
1KFQ_B       465 EHLKTKFqyfeql-------kqgnkADIYDYVDpVDQSVSKNQGVRFVFGDGSRIIFRLSGTGSVGATIRIYFEQFEQQq 537
1KFI_A       465 EHLKTKFqyfeql-------kqgnkADIYDYVDpVDQSVSKNQGVRFVFGDGSRIIFRLSGTGSVGATIRIYFEQFEQQq 537
gi 91776277  430 EHVRAQFaslpgrdf---grytvktCDDFSYTDpVDGSVSQKQGIRILFEDGSRIVFRLSGTGTAGATLRIYLESFEPDi 506
gi 57864860  431 NRLRSMVgdmkgkty---gnyevaySDDFAYTDpVDGSVSEKQGIRIGFTDGSRIVFRLSGTGTKGATLRVYLESYEPDg 507
gi 730308    429 AILREKLatlpgtsy---gnlkvaaADDFAYHDpVDQSVSKNQGIRILFEGGSRIVLRLSGTGTAGATLRLYVERYEPDa 505
gi 151570202 431 DSLRERLsslvgaql---ndekvakADDFSYIDpIDGSVSNHQGIRIIFEDGSRIVFRLSGTGTQGATLRIYLEKYESDs 507
gi 161018469 438 DHLREHLpqagtei----agflvkkADDFTYHDpVDQSVSTRQGIRIFFENGARLVVRLSGTGTCGATLRLYFEQYEGDp 513
gi 68223880  470 DTVENTVvddvpnl----ngvacktIDNFSYTDpIDGSVSTKQGVRVLFEDGSRFVLRLSGTGSSGATIRLYLEQYMDSa 545
gi 163777876 435 TKVRSLIgnqaswptdapsghkiasVDDFEYTDpTNGEVTSKQGLRIIFTDGSRIIFRLSGTGSSGATIRMYIDSYIKDd 514
gi 55275414  430 HSIAAQLpvlpgqtl---agreiliADDFAYADpIDGSVSAHQGLRLLFADGARLIFRLSGTGTEGATLRIYHEHLEKDp 506
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|....
Feature 1                                                    
1KFQ_B       538 iq---------hetATALANIIKLGLEISDIAQFTGRNEPTVIT 572
1KFI_A       538 iq---------hetATALANIIKLGLEISDIAQFTGRNEPTVIT 572
gi 91776277  507 skh-------dqdaQDALADLIAIAHQISELKQRTGRDQPTVIT 543
gi 57864860  508 skh-------dvdtQEALSSLIELAEEIAQIKKFTGRDKPTVIT 544
gi 730308    506 arh-------gietQSALADLISVADTIAGIKAHTADSEPTVIT 542
gi 151570202 508 skf-------siptQQALASLIEIAEDLTNIKSLTGMTEPTVVT 544
gi 161018469 514 rky-------nlnpQEVLQPLQQVALKLLNIQQELGRNRPDIIT 550
gi 68223880  546 tvkshlaektlptaSTALKALIGVALQVSKMESLTGRKTPTVIT 589
gi 163777876 515 aay-------tqdaAVALKPLVELALQLSNLADLTGRTEPSVIT 551
gi 55275414  507 lrq-------hqdpQRTLRDLIQVGRNLSRLETLTGRKTPTVIT 543

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