Conserved Protein Domain Family
ManB

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cd03088: ManB 
ManB is a bacterial phosphomannomutase (PMM) that catalyzes the conversion of mannose 6-phosphate to mannose-1-phosphate in the second of three steps in the GDP-mannose pathway, in which GDP-D-mannose is synthesized from fructose-6-phosphate. In Mycobacterium tuberculosis, the causative agent of tuberculosis, PMM is involved in the biosynthesis of mannosylated lipoglycans that participate in the association of mycobacteria with host macrophage phagocytic receptors. ManB belongs to the the alpha-D-phosphohexomutase superfamily which includes several related enzymes that catalyze a reversible intramolecular phosphoryl transfer on their sugar substrates. Other members of this superfamily include the phosphoglucomutases (PGM1 and PGM2), phosphoglucosamine mutase (PNGM), phosphoacetylglucosamine mutase (PAGM), the bacterial phosphoglucosamine mutase GlmM, and the bifunctional phosphomannomutase/phosphoglucomutase (PMM/PGM). Each of these enzymes has four domains with a centrally located active site formed by four loops, one from each domain. All four domains are included in this alignment model.
Statistics
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PSSM-Id: 100090
View PSSM: cd03088
Aligned: 29 rows
Threshold Bit Score: 601.113
Threshold Setting Gi: 84515086
Created: 21-Oct-2005
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of Candida albicans AGM1 (N-Acetylglucosamine-phosphate mutase) and Pseudomonas aeruginosa PMM/PGM bound to their substrates and divalent metal ions.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          # #  #                                                                        
gi 56122508   16 NFGTSGARGLVVDFTHNVCAAFTHAFLSViekh---fkfdTVAVAIDNRPSSFNIAQACVFAIKQHGYGIEYHGVIPTPA 92
gi 56708489   16 KFGTSGVRGLVSAMTDKICWLYTKAFIQFleqkysiakgtKIAIAHDLRESSPRITTVVIKAIIDSGHEPIYCGEIPSPA 95
gi 1230581    14 AFGTSGVRALVENLTPKLCYAYAQAFLQQics-----natQVAVAIDLRPSSPDIACAMLKAAQDLNIDVVFCGALPTPA 88
gi 69934763   91 AFGTSGARGLVSALTDRVAHGYTQGFLHYlreigefapgtAVALAGDLRPSTPRILRACAQAIRDTGGQPVFCGHVPTPA 170
gi 114775523  49 KFGTSGARGLATDMTDRVCYAYTTAFLHYleskgdirrgdEVAIAGDYRPSSPRIMNAVALAVRDGGFLPVNCGFIPTPA 128
gi 67939214   10 KFGTSGARGLVADMTDRVCYAFASAFLQSvad-----daeTVLLGHDLRPSSPAIASACLSAVKDAGSRVVYAGALPTPA 84
gi 162148911  13 AFGTSGARGLVTAMTDAVCFAYTVGYLKHlatlgefspgtPVAVAGDLRPSTPRIVRACVAAIRSMRGEPVFCGFVPTPA 92
gi 149198840  16 KFGTSGARGLATAMTDEVCYAYTLAFLTHlensnnikegsEVAIAGDLRPSSPRICAAVVKAVEDKGYLAVYSGEIASPA 95
gi 146276015  22 PFGTSGVRGLASAMTDVLCHAYATAFLQYlrqigefapgdPVAIAGDLRESTPRILAACATAVRDCGGEVINCGRTPTPA 101
gi 148559635   4 AFGTSGLRGESVDFTDNVCMVYVSAFLQHlshn---fsyeTVYVGADLRESSPKITISCYRAIELTGRRAIWAGNVPTPA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         ###       #                                                    
gi 56122508   93 LAHYSMQKNIPCIMVTGSHIPFDRNGLKFYRPDGEITKEDELAIVNSEYTFspvgvlp---------------------- 150
gi 56708489   96 VMLYGISNQIPSVMVTGSHIPEDRNGIKFNTPYGEVLKEDEEMIVSQTISIdesifdkngmfl----------------q 159
gi 1230581    89 LAYFAMQNAMPSIMITGSHIPFDRNGFKFYTTTGEITKADELSIQNAPVSErssdfcla--------------------- 147
gi 69934763  171 LAHYAFGRAIPSLMVTGSHIPNDRNGIKFHRPGGEILKSDEAGMARQALALdparfgpdggl-----------------v 233
gi 114775523 129 VACYAMARGQASIMVTGSHIPDDRNGIKFYKPTGEVLKPDEEAISRSEVKLparfgvagevlascfdddgeilpdsmegg 208
gi 67939214   85 IAFYAEKRQAPAVVVTGSHIPFDRNGIKFYRADGEISKADEQAMMSASLDVpetvqpav--------------------- 143
gi 162148911  93 LCVHAFARGIPSLMVTGSHIPADRNGIKFNRAHGEFLKTDEAAMREQDVELpegwfdadgal-----------------a 155
gi 149198840  96 VAYYGFSKTIPSVMVTGSHIPDDRNGIKYNTPLGEILKEDEQGIRAQEVTIpenvfddegffi----------------e 159
gi 146276015 102 LAFRAIGRGMASIMVTGSHIPADRNGIKFYRPHGEVLKSDEAGIVSQVVKAgddrfgadggl-----------------l 164
gi 148559635  81 LAAYAMARNAPAIMITGSHIPEAYNGIKFYRPDGEFLKDDEAPVRGLAEELlskvvdgqrs------------------- 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 56122508  151 ---hletssqGADCYLERYVSLFYSDiLKGKRIGVYEHSSAGRDLYASLFNQLGAEVISLGRSDEFVPIDTEAVSDEDRV 227
gi 56708489  160 klelpepskqAYTQYIDRYVDFFPNNcLAGKTIGLYQHSSVGREIVKEILEKLGAKVILLEFSEKFVSVDTEAIRQEDVK 239
gi 1230581   148 --alppvdlaASFLYQKRYTDLFLSTaLKGKRIGLYEHSGVGRDLITSILRQLGAEVISLDRTDTFVPIDTEAVSEADQQ 225
gi 69934763  234 daaplpepqdIQDGYLRRYLDFFGKDaLSGLVLGLYQHSAVGRDLIARILRALGAEVLPLGRSDRFIPVDTEALRPEDIE 313
gi 114775523 209 cealphvntaAETEYIQRYLDFFPAGcLQGRKVGVYQHSSVDRDIMVAVFEGLGASVTALGRSLSFIPVDTEAIRPEDVQ 288
gi 67939214  144 ---lpqkderAYQAYVARYLDFFGQGaLDGMTVGVYEHSSVARDVLRDILEGLGAQVMSLGRTDTFVPIDTEAVREEDAH 220
gi 162148911 156 iaadlpaitdVVPPFVARYRDFFGPDaLSGLTLGVYQHSAVGRDVLVDIVEALGGKAVPLGRMDSFIPVDTEAVRPEDAA 235
gi 149198840 160 trelaticekAEEIYIKRYTNFFAPDtLAGLRLGVYEHSCVGRDTLKSIYQQLGAEVVGLGRSEKFIPVDTEAIRPEDVA 239
gi 146276015 165 dpaplpevvdVSQDYIDRYVRHFGAGaLEGLTIGVYQHSAVGRDVLVRAVEALGGVAVPFGRSEQFLPVDTEAIRQEDIE 244
gi 148559635 142 -vnlpapladVAEEYVSRSIGAFGRDtLAGMKIGIDLHSAVGRDILVRIFKGLGAEVYPFRRTENFVAVDTEALDPADIS 220
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         # # ##                                 #                  ###  
gi 56122508  228 LAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKALAIPVSCNTAIEESNkfaSIQRTKIGSPY 307
gi 56708489  240 LAKQWASKYKVDSIVSTDGDADRPLVSDEYGNWLKGDILGVLTAKYLQANVIVTPVSSNTVAEKIGyfsNVIRTKIGSPY 319
gi 1230581   226 KAFAWSKEWALDAIVSTDGDADRPLISDENGLWLRGDVVGILVARFLQATHVATPVNANTALELANpnmFTKRTRIGSPY 305
gi 69934763  314 LARGWAAEHRLDAIVSTDGDSDRPLLADHRGEWLRGDILGLICARELGAKCVVTPVSSNTALELSGafaRTIRTRIGSPH 393
gi 114775523 289 LASVWAEKGDFDCLVSADGDGDRPLISDEHGNWLRGDVAGILCARYLEATHVVTPVSSNSAVEKSNwfkQVARTRIGSPY 368
gi 67939214  221 RARAWAKEHTFDAIVSTDGDGDRPLIGDEQGEWLRGDVVGILCAQFLRANVVVTPVSCNTAVEECGafdRVIRTRIGSPY 300
gi 162148911 236 LARDWAAAGTLDAILTTDGDSDRPLLADRAGNWLRGDVLGILAARFLNAAAVTTPVSSNTALELSGfakEVRRTRIGSPF 315
gi 149198840 240 LAAQWAQESHFDAILSADGDCDRPLISDEKGQWLRGDVAGILCAQFLNADAVATPVSCNSAVELCEsfsVVKRTRIGSPY 319
gi 146276015 245 LAAQWARSNKVDAIISTDGDSDRPLVADASGTWLRGDIVGLLCARAVGADIVVTPVSSNTVLELSGaapRVARTRIGSPY 324
gi 148559635 221 RARTFIAEHGLDAVVSTDGDGDRPLVIDDQGRQVNGDTLGILTARYLRAKTVVTPLSTTSALEESGwfeNIHRTRIGSPY 300
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                # # #                                                   
gi 56122508  308 VIAAFadl------akQFDSVAGFEANGGFLLASDLqinDKELKSLPTRDAVLPALMLLIASRn--sTISQLINNLPQRF 379
gi 56708489  320 VIAAMnel-----lsnNQNAVVGYEANGGFLLASDIckdDKTLKALPTRDAVIPMLAVMMLSInsnkTVSELLFDLPSRY 394
gi 1230581   306 VIEAMqalalqattttGSPKVVGYEANGGFLVGNDMpcgLGMLNALPTRDAVLPILAVLCQSAqdglPISQLVDGLPARY 385
gi 69934763  394 VIAAMdea-----aceTLGPVCGYEANGGFLLGSEIvrdGRRLSALPTRDAVLPVVAVLAAAKa--rPLAELCADLPQRA 466
gi 114775523 369 VIEAMdma-----vadGASSVVGYEANGGFLIATDIelhGKTLTALPTRDAVIVPVAILLLAIeggvSVGGLLDSLPQRF 443
gi 67939214  301 VIAGMee-------agSDGVVVGYEANGGFLLGSDAvrnGKALDALPTRDAVLPIVALLCMVRelgcKVSELTDSLPKRY 373
gi 162148911 316 VVAAMtea-----aeaGLVPSVGYEANGGFLLASDVtryGRTLAALPTRDSVLPMICALVAAReqgrDLDALVATLPPRF 390
gi 149198840 320 VIASMkea-----sasGSQMVVGYEANGGFLTNSDFkqgAKVLEALPTRDAAILHIALLVDAKkqgiKLSELVATLPARY 394
gi 146276015 325 VIAAMmdaa----tadPSARVCGYEANGGFLLGTDVgegEGRLTALPTRDALLPIIAVVRAARq--rPLADILAELPPRF 398
gi 148559635 301 VVVEMar--------aVAHPVVGFEANGGFLLGDDValkTGLLRRLPTRDAVLPAVAVLAQAKdqgmRLSEMVATLPSRF 372
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                         # ###    #     
gi 56122508  380 TWSDRVKNFPSDSSQQIIKNAISspnnffns-----lgyeslsCSSIDETDGARFTLNNGDIIHLRPSGNAPELRCYAEA 454
gi 56708489  395 TASSKIDDFASEKSQEILKSILAgesdlldkii-sehfdgknsIENIDTTDGVRVTLTNQDIIHLRPSGNAPELRCYTEA 473
gi 1230581   386 TYSDRIQQVPTEFSQRLIKQLKEdsqqmqcfl--eatgveapsVDTVDETDGLRIHLSNYEIVHLRPSGNAPELRCYAES 463
gi 69934763  467 TFSDRLKDFPTRDAQAILAWLTSgseaesrarieaqfgalagrLGRIDQTDGLRMSFEDGAIIHLRPSGNAPELRLYTEA 546
gi 114775523 444 SCSDRLKAFPTDIAQARIKALSSgdiatdkaaiealfgeqfgtVASINDTDGLRITFASEEVVHLRPSGNAPELRCYNEA 523
gi 67939214  374 TASDRLQDFPTELSRSIIAGLQAapdeigal-----mapdagaAAAVDSTDGLRVTFANGDIVHLRPSGNAPELRCYAEA 448
gi 162148911 391 TLSDRLVEMPTAQSQAQIAKLGEdpeggaaal---gfteacgpLHHVDKTDGLRMIFANGEIIHLRPSGNAPELRVYVEA 467
gi 149198840 395 TISDRLKEFPTERSQEKLKSFTSeggesriee---lfaqlsgsLASIDQTDGVRMTFDNKEVIHLRPSGNAPELRCYNEA 471
gi 146276015 399 TFSDRVAEFPQAHSAALIAFLSTgpedaqlariarcfgeiaglPIGIDTTDGYRMTFADGSIIHFRPSGNAPELRCYTEA 478
gi 148559635 373 MKADRVKEVPGDRAAPFLHAMETsqsfrsn------fspliaePEAISTVDGVRMAFANGDTVHFRQSGNAPEMRIYIET 446
                        490
                 ....*....|....
Feature 1                      
gi 56122508  455 SDENQAKQYVTNVL 468
gi 56708489  474 ASDEQAKSLNQYCV 487
gi 1230581   464 SEMNRAKQIVELAL 477
gi 69934763  547 ETEETARTLNARAL 560
gi 114775523 524 ATEARAAEMNRICM 537
gi 67939214  449 DTAERAGRLCRECL 462
gi 162148911 468 DSPERADELLKTGL 481
gi 149198840 472 DSEARAIELNRACI 485
gi 146276015 479 ETEAKARDLNARAL 492
gi 148559635 447 DSAEKTDRMLSEFI 460

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