Conserved Protein Domain Family
PMM_PGM

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cd03089: PMM_PGM 
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The phosphomannomutase/phosphoglucomutase (PMM/PGM) bifunctional enzyme catalyzes the reversible conversion of 1-phospho to 6-phospho-sugars (e.g. between mannose-1-phosphate and mannose-6-phosphate or glucose-1-phosphate and glucose-6-phosphate) via a bisphosphorylated sugar intermediate. The reaction involves two phosphoryl transfers, with an intervening 180 degree reorientation of the reaction intermediate during catalysis. Reorientation of the intermediate occurs without dissociation from the active site of the enzyme and is thus, a simple example of processivity, as defined by multiple rounds of catalysis without release of substrate. Glucose-6-phosphate and glucose-1-phosphate are known to be utilized for energy metabolism and cell surface construction, respectively. PMM/PGM belongs to the alpha-D-phosphohexomutase superfamily which includes several related enzymes that catalyze a reversible intramolecular phosphoryl transfer on their sugar substrates. Other members of this superfamily include phosphoglucosamine mutase (PNGM), phosphoacetylglucosamine mutase (PAGM), the bacterial phosphomannomutase ManB, the bacterial phosphoglucosamine mutase GlmM, and the phosphoglucomutases (PGM1 and PGM2). Each of these enzymes has four domains with a centrally located active site formed by four loops, one from each domain. All four domains are included in this alignment model.
Statistics
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PSSM-Id: 100091
View PSSM: cd03089
Aligned: 93 rows
Threshold Bit Score: 379.165
Threshold Setting Gi: 159475048
Created: 21-Oct-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2FKM_X; Pseudomonas aeruginosa PMM/PGM binds alpha-D-glucose 1,6-bisphosphate and Zn; defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:2H4L_X; Pseudomonas aeruginosa PMM/PGM binds ribose 1-phosphate and Zn; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          # #  #                                                                        
2FKM_X        12 IFRAYDIRGVVGd-------tLTAETAYWIGRAIGSEslar--------gepCVAVGRDGRLSGPELVKQLIQGLVDCGc 76
gi 67855915    9 LQNGSDIRGVALegipgesvnLTPEIAKILGQAFISWlekklg---ksasklIISLGRDSRLSGPDLLAAASEGISLLGs 85
gi 145341312   6 LQNGSDIRGVALdgvegeaitLDAATASAIGRAFADWlrarnp----nakdiSVGVGRDPRLSGETLRDGMFAGMVEGGa 81
gi 146346315   8 LQNGTDIRGIATknpe-kevnLTVERVKLITRGFIKWiknkkn---lennkiKIAIGIDSRLSGLEFKRCICEELLNLGc 83
gi 159475048   1 LQNGSDIRGIALegvpnepvtLSAGAVFFIGVAFARWlrskg------hsapKVSVGRDPRLSGPLLESAFAAGLIHGGa 74
gi 160933572   8 FKSGTDIRGVASegvagqkinLTDEVMEKISAGFVLWlskkan---kpadqlIISVGHDSRISASRIQAAVTRALEPAGv 84
gi 162676096  24 LQNGSDIRGVAVdgvegepvnLTEAVAEAIGAAFAAWlldkkna--dfstklRVSLGTDSRVSAKSIQSAVARGISGAGl 101
gi 126699936   7 LQNGTDIRGIAYends-kevnLTVEEVKKIAKAFHIWlkekt-----kkdkvTVAIGTDSRITGSQFRSTVIETLTNDDc 80
gi 18418333   60 LQNGSDIRGVAVtgvegepvsLPEPVTEAIAAAFGQWllhkkk---aesrrlRVSVGHDSRISAQTLLEAVSRGLGVSGl 136
gi 29375275    7 LQNGSDIRGIALdtee-qtatLTATAVAEIAVGVVRWlqdkkqlprkaqqrlTIAIGHDSRLTAEPIKQALVDTFLSLGi 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             ###       #                                
2FKM_X        77 -qVSDVGMVPTPVLYYAANVle------gkSGVMLTGDHNPPDYNGFKIVVaGETLANEQIQALRERIEKndla------ 143
gi 67855915   86 -tVYNFEIASTPAMFMSTINpdl----scdGAIMLTASHLPFNRNGLKFFTkNGGLNKGDITEILNLAENnnfpv----- 155
gi 145341312  82 -rVIDMGLATTPACFMACVTega----kydGSVMLTASHLPFNRNGMKFFTsEGGLDKPDITDICARAAAyveagv---- 152
gi 146346315  84 -yVYDCGLCTTPAMFMTTVTgsy----rchGAIMITGSHLPYYYNGLKFFTeSGGCEKEDIKYILNRALNkydns----- 153
gi 159475048  75 avVHLFGLATTPAMFYSIVLsgergdegggGGDYIHDYHLPYNANGLKFFVaAGGLDKPDIAELLQTAAAaaaeagshll 154
gi 160933572  85 -tVYDCGLASTPSMFMTTVDlk------cdGAVQITASHHPFNRNGLKFFTpDGGLESDDITALLALAEKgdkpe----- 152
gi 162676096 102 -dVIEYGMASTPAMFNSTLTvrddlfcpvqGAIMITASHLPYNRNGLKFFTsDGGLGKKDITDILSRAEKiyhvmsnest 180
gi 126699936  81 -nVIDCEIATTPAMFMTTIMdgy----nsdGAIMITASHLPYYYNGLKFFTeSGGLEKTDIKEMLDIAVKndsvdy---- 151
gi 18418333  137 -dVVQFGLASTPAMFNSTLTedesflcpadGAIMITASHLPYNRNGFKFFTsDGGLGKVDIKNILERAADiykklsden- 214
gi 29375275   86 -qVIDVGLATTPAMFMATQFptl----qcdAAIMITASHLPYYFNGLKFFTaEGGAEKEDIRYILSHTDPlta------- 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2FKM_X       144 -----sgvGSVEQVDILPRYFKQIRDDIama-----------kpmKVVVDCGNGVAGVIAPQLIEALgc-svIPLYCEVD 206
gi 67855915  156 ----gtekGNIKDYDFISVYSEGLVKKVregvnhtenfeqplkglKIIVDAGNGAGGFYAEKVLKPLgadteGSQFLDPD 231
gi 145341312 153 --gsvtapSGVVSAPFLPTYAAQLCDIIrkgvnftdnydkpltgmKIVVDAGNGSGGFFADMVLEALgadtsGSQFLDPN 230
gi 146346315 154 ----vhsrGNIFKIDFIDEYSGILVKKIreginskvnynrplselKIIVDAGNGAGGFFANKVLEPLgakieGSQFIIPD 229
gi 159475048 155 avslsldpARLARLPFLPTYSASLRDLIkrgvnspanyhfpllgcHVVVDAGNGAGGFFAEQVLAPLgadtsGSVFLDPD 234
gi 160933572 153 ----nvpgGSVKPVAYMRDYAKHLREIIckgvnaad-ydhplkgfHIVVDAGNGAGGFYASDVLEPLgadvaGSQFLDPD 227
gi 162676096 181 eaaaavadKIVHSVDYMEQYTADLVDAVrkgaggv---erplegfHIVVDAGNGAGGFFAGRVLEPLgavttGSQFLDPD 257
gi 126699936 152 --eevnkkGEVIVKNLIEDYSNLLIDKIrkgvnssknyekpfsglKILVDAGNGAGGFFAEKVLHILgadttGSQFLNPD 229
gi 18418333  215 lrksqresSSITKVDYMSVYTSGLVKAVrkaagdl---ekplegfHIVVDAGNGAGGFFAAKVLEPLgaitsGSQFLEPD 291
gi 29375275  154 -----nenGTLMKQELLPIYAEHLVEKIrqgihspe--ekplqgfRIIVDAGNGAGGFFAEQVLQVLgadttGSQFLEPD 226
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          # # ##                                     #  
2FKM_X       207 GNFPNHHPDPgKPENLKDLIAKVkaenADLGLAFDGDGDRVGVVTntgTIIYPDRLLMLFAKDvvsrnpGADIIFDVKCT 286
gi 67855915  232 GTFPNHIPNPeNEEAMESICRAVidnkADFGIIFDTDVDRGAAVDnfgKELNRNRLIALISAMilkehpGSTIVTDSITS 311
gi 145341312 231 GNFPNHSPNPeDKEAMAAGVQCVlnakADLGIVFDTDVDRSAVIDssgKEINRNKLIALLSEIvlkespGATIVTDSVTS 310
gi 146346315 230 GKFPNHLPNPeDKEAMHSIKEAViknkADLGIIFDADVDRAAIVDcsgREINRNALIALISTIvleeypGTTIVTDSVTS 309
gi 159475048 235 GTFPNHPPNPeHPAAMASGAAAVkasaAELGIVFDTDVDRSAIVDasgREINSNRFIALMAAVvlrqhpGTTVVTDSVTS 314
gi 160933572 228 GMFPNHIPNPeNEEAMASICEAVkeqkADLGVIFDTDVDRGGAVDhqgEEINRNRLVAVASAIalegneGGTIVTDSITS 307
gi 162676096 258 GMFPNHIPNPeDKKAMESITKAVldqkADLGIIFDTDVDRAAAVDssgKELNRNRLIALLAAIvleqhpGTTIVTDSVTS 337
gi 126699936 230 GMFPNHIPNPeNKEAMESICKAVldnkSDLGIIFDTDVDRAAIVGkngKPINKNALIAVISSIvleehpKTAIVTDSITS 309
gi 18418333  292 GMFPNHIPNPeDKAAMEAITKAVldnkADLGIIFDTDVDRSAAVDssgREFNRNRLIALLSAIvleehpGTTIVTDSVTS 371
gi 29375275  227 GHFPNHVPNPdNSEAMKSIQTAVlanqADLGIIFDTDVDRSAVVDqsgEVLNRNNLIAVLAAIvlkeapGSYIVTNSPTS 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           ###                     # # #                                
2FKM_X       287 RRLIALISGy-GGRPVMWKTGHSLIKKKMKE-----TGALLAGEMSGHVFFKerwFGFDDGIYSAARLLEILSqd----- 355
gi 67855915  312 DGLSEFITQelNGVHHRFRRGYKNVINEAIRlnnegQESWLAIETSGHGAMKe-nYFLDDGAYLVTKLLIKLAncqq--- 387
gi 145341312 311 DGLHKFITAk-GGKHVRFMRGYKNVINKGKElnasgVVTPLMIETSGHGAMKe-nYDLDDGAYLAVKIIIEAVrrrl--- 385
gi 146346315 310 DGLSEFIQEl-GGKHHRFKRGYRNVINEGIRlnnqnEGCYLAIETSGHAAFKe-nYFLDDGAYLIAKVLIKMArlks--- 384
gi 159475048 315 NGLTDFITAl-GGKHMRYKRGYKNVIGAGVRlnaqgEDCALMMETSGHGALRe-nFFLDDGAYLAVKAIIEHVrrkqe-- 390
gi 160933572 308 SGLKSYIEEtlGGKHHRFKRGYKNVINEAIRlnqegINCPLAIETSGHAALRe-nYFLDDGAYLVTKIIIKMAqlkk--- 383
gi 162676096 338 DGLTTFIEQklGGKHHRFKRGYKNVIDEGIRlntvgEETHLAIETSGHGALKe-nHWLDDGAYLMVKLLIKLAaakvsge 416
gi 126699936 310 EGLAKFINEl-QGRHHRFKRGYKNVINEAIRlnsegEECHLAIETSGHAALKe-nYFLDDGAYLIAKILIKVAklsl--- 384
gi 18418333  372 DGLTSFIEKklGGKHHRFKRGYKNVIDEAIRlnsvgEESHLAIETSGHGALKe-nHWLDDGAYLMVKILNKLAaaraagq 450
gi 29375275  307 SHLKTFIEEk-GGQQIRYISGYRNVINKMIElnhggFQTPLAIETSGHAAFQe-nYNLDDGAYVVAKILMLLPelkq--- 381
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2FKM_X       356 ---qrDSEHVFSAFPSDISTPEINITvte-------dsKFAIIEALQRdaqwge----------gnittLDGVRVDYp-- 413
gi 67855915  388 --egkNLAEFIAKLREPEESQEFRVKintes---fqdyGNQVIEKLKEfvitqdd-------wqlvpnnYEGVRISCcsg 455
gi 145341312 386 -anepSIGQVLETLEEPLEEAEVRLKiadpd---fkayGLNVIENLLAavndpahslf--gkckpapdnYEGLRVCVdeg 459
gi 146346315 385 --egkSIEDLIQNMKMPVESLEFRFDild-------enFKEYGDKILEelklyiekv---egwsevspnYEGIRINCcsf 452
gi 159475048 391 -gaagGLAELLAGLAEPAESREWRVRiqh-------tdFKAVGGRVLAafhdwgavpaapsswslesvnHEGWRVNMdeg 462
gi 160933572 384 --egkTLESLLAPLQEPKESVEVRMPilted---fracGEQVISGLEAyakvhdg-------wqiapdnHEGIRVSFgkd 451
gi 162676096 417 asgskVLSDLIADLEDPAISSELRFKidqshpdigsggFKAYGEQVLDkldqlvasd---anlkkapvnHEGIRVSGy-- 491
gi 126699936 385 --egkTIEELIENLEEAQEEKEVRIGink-------edFRPYAEDILKdldshvksi---dgwsvapnnFEGIRVNCdes 452
gi 18418333  451 gsgskVLTDLVEGLEEPKVALELRLKidknhpdlegsdFREYGEKVLQhvsnsietn---pnliiapvnYEGIRVSGf-- 525
gi 29375275  382 --nnqTLGDLIATLKQPAETNEFRFKitaed---vtcyGQQVLRDFELfvenqad-------favdrenQEGVRGNVsgq 449
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
Feature 1                 # ###                           
2FKM_X       414 ---kGWGLVRASNTTPVLVLRFEAdteeeLERIKTVFRNQL 451
gi 67855915  456 -denGWLLLRLSLHDPVIPLNVESnvsggVKKIANRVLEFL 495
gi 145341312 460 dgrqGWFLLRCSLHDPVMVLNFESqtqcgVQTMAEEFGTWI 500
gi 146346315 453 -kgsGWLLLRLSLHEPVLCLNIECdlsgySNIIIDKLKSFL 492
gi 159475048 463 egrrGWLLLRQSLHDPLLVLNVESelpggAAEAAVRVAAWL 503
gi 160933572 452 -egdGWFLLRLSVHDPIMPLNIESdavggVKLIAGKLYDYV 491
gi 162676096 492 ---gGWFLLRLSLHDPVLPLNIEGpsmeaTKKLGEFVLSAV 529
gi 126699936 453 -ngdGWFLLRISLHEPLLALNIESnkiggAEQIYSKLKLFL 492
gi 18418333  526 ---gGWFLLRLSLHDPVLPLNIEAqseddAVKLGLVVATTV 563
gi 29375275  450 -ygsGWFLLRLSLHEPLLVLQVENdqsdkNACVIEKIATFL 489

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