1MR9,1XAT,1KHR


Conserved Protein Domain Family
LbH_XAT

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cd03349: LbH_XAT 
Click on image for an interactive view with Cn3D
Xenobiotic acyltransferase (XAT): The XAT class of hexapeptide acyltransferases is composed of a large number of microbial enzymes that catalyze the CoA-dependent acetylation of a variety of hydroxyl-bearing acceptors such as chloramphenicol and streptogramin, among others. Members of this class of enzymes include Enterococcus faecium streptogramin A acetyltransferase and Pseudomonas aeruginosa chloramphenicol acetyltransferase. They contain repeated copies of a six-residue hexapeptide repeat sequence motif (X-[STAV]-X-[LIV]-[GAED]-X) and adopt a left-handed parallel beta helix (LbH) structure. The active enzyme is a trimer with CoA and substrate binding sites at the interface of two separate LbH subunits. XATs are implicated in inactivating xenobiotics leading to xenobiotic resistance in patients.
Statistics
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PSSM-Id: 100040
View PSSM: cd03349
Aligned: 95 rows
Threshold Bit Score: 126.505
Threshold Setting Gi: 68548787
Created: 21-Jul-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active streptogramin acetyltransferase is homotrimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Structure:1KHR_A; Enterococcus faecium streptogramin acetyltransferase Vat(D) binds CoA and substrate Virginiamycin; defined at 3.5A contacts. Shown are two active sites within the trimer.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  # #                                         ## #          ##          
1MR9_Z        29 NVEVGE--YSYYDskngetfdkQILyhypilndkLKIGKFCSIGPGVTIIXngaNHRXd---gSTYPFNLfgngwek--- 100
1KHR_A        29 NVEVGE--YSYYDskngetfdkQILyhypilndkLKIGKFCSIGPGVTIIMngaNHRMd---gSTYPFNLfgngwek--- 100
gi 90417240  120 SIVMGK--RSYISgp------sFVRgg-----grFLVGSYCNLAENLKIFTgsdAHPLnh--aAMMNFHGnsrlvedg-l 183
gi 94314645   66 PAFIGG--FTYVNv-------gAVVfp------hVRIGKFCSIGRNVQIGAa--RHPVef--lSSHPFQFspalfnrvpg 126
gi 144897697  80 DSFFGLgsALYIGr-------hSHMlgngmispmLTIGRHCSISQNVVLGGg--RHPMeylttGHIPGAGeersyya--- 147
gi 126737659  16 KIEIGR--FTHGYetv----nfRNWgqg----anVQLGSFCYFSENIQVILd-pQSPFdr--iAGFPFGKvfqeelg--- 79
gi 84324411   54 ALEVGA--YTDVVs-------gCEFle------vASIGRFCSIATGVVIGQprrSHPMhw--lSTHAFTAnpkll----- 111
gi 68548787   37 KTFYMAp-GSHVSk-------dLIAgk------ySFIGSECMIGPKVKLGNyvmFAPRv---aVVGADHIfnksgr---- 95
gi 118052969  35 VSWFGF--ASYGNn-------iTVRs-------yVEVGRYCSIGRDVILGLg--HHDYtn--iSTSPYFEkvgfnn---- 90
gi 156860994  45 NSIIGD--YSYVNy-------nSIIh-------cCHIGKFCSIGPNVVAGLg--NHPVek-nvTTSPRLFlkgkf----- 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              #  #                ##    #       # ## #   #              
1MR9_Z       101 -hxpkldQLPIKg-DTIIGNDVWIGKdVVIXPGVKIGDGAIVAANSVVVKDIAPYXLAGGNPANEIKQRFdqdtiNQLLD 178
1KHR_A       101 -hmpkldQLPIKg-DTIIGNDVWIGKdVVIMPGVKIGDGAIVAANSVVVKDIAPYMLAGGNPANEIKQRFdqdtiNQLLD 178
gi 90417240  184 smnltykELPDStsCVEMGNDVWIGRdVTLKNGVNVGDGCVIGQHSLVLNDCLPYGVYVGSPARLIRFRFpehtiQDLCS 263
gi 94314645  127 ypalqteKWRFHa-PTTVGNDVWLGTnCVIVAGVKIGDGAVVGAGAVVTKDVAPYEIVGGVPARRIRLRFdedkvSRLLK 205
gi 144897697 148 daedhfaAGDTSa-FTRIGCDVWIGAnAMVLRGRTIGTGACIGGGAVVTHDVPPYAVVVGNPAKVIRFRFpdkiiESLLR 226
gi 126737659  80 -gedlpiQKDCEw-DVSIGNDVRIGDgVTIMSGVTIGNGAVIAANSTVFNDVGAYEIWGGNPATFLQKRFddeliSALEE 157
gi 84324411  112 --rkplqPEREAt-PARIGHDVWIGRdALILDGVEIGTGAVIGAQSLVNRDVPPYAVVAGSPARVLRYRFapeliERLLA 188
gi 68548787   96 --piifsGRPEMp-LTIIEDDAWVGYgSILMAGIRIGRGAIVAAGAVVTKDVPAYEIHGGVPAKKIGIRFqt-ieDCQFH 171
gi 118052969  91 -nkiklaCENPKr-RVIIGNDVWIGDkAMVVSGVSVGDGCVIAAGSVVTKDVPPYSIVAGVPAKVIKNRFpdeviSELID 168
gi 156860994 101 ---lledRYDQFa-IVTIGNDVWIGAnVTIVNGVTIGDGAVIGANSIVTKDIPPYSIYGGVPAKCIRMRFeqnqiDFLLK 176

                 ....*..
Feature 1               
1MR9_Z       179 IKWWNWP 185
1KHR_A       179 IKWWNWP 185
gi 90417240  264 LKWWDWP 270
gi 94314645  206 TRWWDLP 212
gi 144897697 227 TRWWTLP 233
gi 126737659 158 LAWWNLP 164
gi 84324411  189 SRWWELP 195
gi 68548787  172 DKMLALP 178
gi 118052969 169 IKYWAYD 175
gi 156860994 177 LKWWNMD 183

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