Conserved Protein Domain Family
LbH_THP_succinylT

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cd03350: LbH_THP_succinylT 
Click on image for an interactive view with Cn3D
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate (THDP) N-succinyltransferase (also called THP succinyltransferase): THDP N-succinyltransferase catalyzes the conversion of tetrahydrodipicolinate and succinyl-CoA to N-succinyltetrahydrodipicolinate and CoA. It is the committed step in the succinylase pathway by which bacteria synthesize L-lysine and meso-diaminopimelate, a component of peptidoglycan. The enzyme is homotrimeric and each subunit contains an N-terminal region with alpha helices and hairpin loops, as well as a C-terminal region with a left-handed parallel alpha-helix (LbH) structural motif encoded by hexapeptide repeat motifs.
Statistics
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PSSM-Id: 100041
View PSSM: cd03350
Aligned: 20 rows
Threshold Bit Score: 144.831
Threshold Setting Gi: 139437269
Created: 10-Oct-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active THP succinyltransferase is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Citation:PMID 9671504
  • Comment:The structure evidence shows only the interaction of CoA and substrate to one interacting LbH subunit/surface.
  • Structure:1KGQ_A; Mycobacterium bovis THP succinyltransferase active site surface A binds the ligands, succinamide-CoA and L-2-Aminopimelate; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #       #           #     #           #      #        # #      # ##  #        
1TDT_C      102 GFRVVPPATVRQGAFIARNTVLM-PSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQanPTIIED 180
1KGQ_A      102 GFRVVPPATVRQGAFIARNTVLM-PSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQanPTIIED 180
gi 83815870  99 VRLVPGGSAIRTGAYVAPGVVCMpPMYVNVGAYVDAETMIDSHALVGSCAQIGKRVHLSASAQVGGVLEPVHatPVIVED 178
gi 76874061  69 VRMVPGGSSVRRGAYVAKGTIIMpPAYVNIGAYIDEGTMVDSHALVGSCAQVGKNVHLSAAVQLGGVLEPIGasPVVVED 148
gi 55229847  99 TRNTPDGTTIRRGAYLGEDCIMMsPSFVNIGAYVGDGTLVDSCDTVGSCAQIGENVKLGANTLIGGVLEPVEnaPVIVED 178
gi 15644267  90 KARIEPGAIIRDMVEIGEGAVIMmGAVINVGAVIGEGTMIDMNAVVGGRAIIGKKCHIGAGAVIAGVIEPPSakPVVIED 169
gi 18145573  92 NARIEPGAIIRDRVSIGDNAVIMmGAVINIGAEIGESTMVDMNAVIGARGKLGKRVHLGAGAVVAGVLEPPSktPCIIED 171
gi 20093873 100 DVRIEPGAIIREKVKLGKGVVVMmGAVINIGAKIGDGTMVDMNAVVGSRAEVGKNVHIGAGAVIAGVLEPPSakPVVIED 179
gi 2633789   91 KARIEPGAIIRDQVEIGDNAVIMmGASINIGSVIGEGTMIDMNVVLGGRATVGKNCHIGAGSVLAGVIEPPSakPVVIED 170
gi 94969114  97 VRVVPGGSTVREGAYLAASVVCMpPMYVNVGAYVDEGTMIDSHALVGSCAQIGKRVHLSAAAQVGGVLEPINatPVVIED 176
                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
Feature 1           ###    #          ##                                             
1TDT_C      181 NCFIGARSEVVEGVIVEEGSVISMGVYLGqstriydr------etgeihyGRVPAGs----VVVSGNLP 239
1KGQ_A      181 NCFIGARSEVVEGVIVEEGSVISMGVYLGqstriydr------etgeihyGRVPAGs----VVVSGNLP 239
gi 83815870 179 DVFVGGNAGLYEGCIVRAGAVLAAGVTLTsstrlydlaeetvhtasddepLVVPENa----VVVPGSRS 243
gi 76874061 149 DAFIGAGCVIVEGVVIKKGAVLAPGVRLSatipvydcv----nerqldkgEPIPEYa----IVIPGSRP 209
gi 55229847 179 EVSLGAGCRVTSGFVVGEGSIVGENTLLT---------------------PRIPVYdlvedEVLYGELP 226
gi 15644267 170 EVLVGANAVILEGVTVGKGAVVAAGAVVT---------------------KDVPPY-----TVVAGVPA 212
gi 18145573 172 DVLIGANAVILEGVKIGKGSVVAAGSVVV---------------------EDVPAG-----VVVAGTPA 214
gi 20093873 180 DVVIGANAVILEGVRVGKGAVVAAGAVVT---------------------EDVPPS-----KVVAGVPA 222
gi 2633789  171 DVVIGANAVVLEGVTVGKGPVVAAGAIVV---------------------NDVEPY-----TVVAGTPA 213
gi 94969114 177 DVLVGGNCGVYEGTIVHERAVLGAGTILTrstplfdivreeiyrssaegpLEVPAGa----VVVPGSRA 241

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