Conserved Protein Domain Family
LbH_UDP-GlcNAc_AT

?
cd03351: LbH_UDP-GlcNAc_AT 
Click on image for an interactive view with Cn3D
UDP-N-acetylglucosamine O-acyltransferase (UDP-GlcNAc acyltransferase): Proteins in this family catalyze the transfer of (R)-3-hydroxymyristic acid from its acyl carrier protein thioester to UDP-GlcNAc. It is the first enzyme in the lipid A biosynthetic pathway and is also referred to as LpxA. Lipid A is essential for the growth of Escherichia coli and related bacteria. It is also essential for maintaining the integrity of the outer membrane. UDP-GlcNAc acyltransferase is a homotrimer of left-handed parallel beta helix (LbH) subunits. Each subunit contains an N-terminal LbH region with 9 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X), and a C-terminal alpha-helical region.
Statistics
?
PSSM-Id: 100042
View PSSM: cd03351
Aligned: 119 rows
Threshold Bit Score: 313.215
Threshold Setting Gi: 53802408
Created: 16-Aug-2001
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active UDP-N-acetylglucosamine acyltransferase is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Comment:The structure evidences show only the interaction of substrate to one interacting LbH subunit/surface.
  • Structure:2QIV_X; Escherichia coli UDP-N-acetylglucosamine acyltransferase binds UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc; defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:1J2Z_A; Helicobacter pylori UDP-N-acetylglucosamine acyltransferase binds octyl thioglucoside; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                          #  #          
1J2Z_A         3 KIAKTAIISPKAEINKGVEIGEFCVIGDGVKLDEGVKLHNNVTLQGHTFVGKNTEIFPFAVLGTQPQDLKYKGey-seLI 81
2QIV_X         7 FVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVLKSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGep-trVE 85
gi 30468068    9 TIHPTALVHPGAQIGKNVSIGAYSVVGAYVWIGDDTKIGSHVMIDGKTYIGKANQIMCFCAIGVVPQDLKYKHeg--mTY 86
gi 88805697    1 MNQPLAYVHPGAKIAKNVVIEPFATIHNNVTIGEGSWIGSNVTIMEGARIGKNCNIFPGAVISAPPQDLKYQGee-ttVE 79
gi 60491812    1 MISPLAYIHPEAKIGENVEIAPFVYIDRNVVIGDNNKIMANANILYGSRIGNGNTIFPGAVIGAIPQDLKFKGee-stAE 79
gi 89255946    1 MIHSLAVVHESAKIADSAIIGPFCVIGKNVVIGENTELKSHVTIGDNAVIGKNNRIFQYASIGDDPIDYTYKKgdfsqVV 80
gi 34539941    6 KISPLAWVDPHAEIGVGVEIGPFAVVEAGAKIGDGSILHPHAVVRYGSTLGKGCEIHPNAVIGGVPQDLKFQGed-ttAI 84
gi 14285564    3 NIHSTAIVHPAASLGRNVVVGPYSIIGSDVSIGDYTRIGPHVVITGKTVIGCNNQILSGCILGSVPQDLRYIDseltgLY 82
gi 60491183    1 MISPLASIAPGAKIGKNVIIQPFAYIEDNVEIGDDCIIMPYASVLNGTRLGKGNKVYQHAVLGAEPQDFHYKGee-ssLI 79
gi 154494233   1 MISPLAYVDASAKLGANVTVHPFAYIDKNVEIGDDCEIMPHVSIMSGTRMGKRNRIFNGAVIAAEPQDFNYKGdd-tiVE 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     #                      #  #                  #               ##    
1J2Z_A        82 IGEDNLIREFCMINPGTEggikkTLIGDKNLLMAYVHVAHDCVIGSHCILANGVTLAGHIEIGDYVNIGGLTAIHQFVRI 161
2QIV_X        86 IGDRNRIRESVTIHRGTVqggglTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVHQFCII 165
gi 30468068   87 IGNGNFIREYVSIHRATKg---vTYIGNENLLMAYTHIAHDCQISDHVILVNGVNLGGHVRIGRYAVIGGLTGLHQFVEI 163
gi 88805697   80 IGNNVTIRECATINKGTSdr-mkTVIGKNCLIMAYCHIAHDCVVGNNCIFSNNSTLAGHVTIGDYVVLAGLVAVHQFVSI 158
gi 60491812   80 IGDNNLIRENVTINRGTAak-grTIVGNNNLLMEGVHVAHDALIGNGCIVGNSTKMAGEIIIDDNAIISANVLMHQFCRV 158
gi 89255946   81 IGDNNIIRECATIHGGTAkeigvTSVGNNNIIMCYVHIGHDCKMGSYINLVNGVGLAGHVHIDDYAILSSNVGVHQFCRV 160
gi 34539941   85 LGDYTIVRECATVNRGTAsr-gtTVVGSHCLLMAYSHIAHDCVLGDHIIVGNASQIAGEVEIDDHAIISGGVLIHQFVRI 163
gi 14285564   83 IGNNNLIRENVTVHRASGng--vTYIGNNNLIMVNCHVAHDCQIRNNIIISNSVSLAGHVIIDSCVIIGGHAGLHQFVHV 160
gi 60491183   80 IGDNNHIRENVVISRATFgg-naTKIGNGNFLMDKVHICHDVQIGDNCVAGIGTTIAGECTLDDCVILSGNVTLHQYCHV 158
gi 154494233  80 IGDDNVIRENVVINRATNsg-gkTVIGNGNFLHEGVHVSHDTHIGNHSVFGYGSKISGNCMIEDYVIFGGNVLVSQGCRV 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                          #     ##                                      
1J2Z_A       162 AKGCMIAGKSALGKDVPPYCTVEGnra--firGLNRHRMRQLles----kdIDFIYALYKRLFrp-------ipSLRESA 228
2QIV_X       166 GAHVMVGGCSGVAQDVPPYVIAQGnha--tpfGVNIEGLKRRgfs---reaITAIRNAYKLIYrs-------gkTLDEVK 233
gi 30468068  164 GRLSMIAGMSRIDRDVPPYMIAEGnpa--rlrGINLVGLTRRk-------vEEAHKSALKKMWmk--------mKKREGI 226
gi 88805697  159 GTHAFVTGGSLVRKDVPPYVKGARepm--syvGINSVGLRRRgft---aekIREIQNIYRILYqr-------nyNNSQAV 226
gi 60491812  159 GGYVMIQGGCRFSKDIPPYIIAGRepi--aysGINIIGLRRRgfs---neiIENIHNAYRIIYqs-------glNTSDAL 226
gi 89255946  161 GKHAFIAHAALVGKDVPPYLMVTAvnagstpcGINTEGLKRRgft---peeMKKIKEVYKVLYrk-------glMMKEAF 230
gi 34539941  164 SQHVMIQGGSRLSKDIPPYVLVGRdpl--vycGINIVGLRRRnft---neqIFLINDIYRTLYqr-------glNNSDAI 231
gi 14285564  161 GALSMIAAMSKIEKNVLPFVVVSGmpa--itrTINLVGLKRYgisktdinyIRLMLENLKVQPlafdtysifkkFKSDNL 238
gi 60491183  159 GQWTLVQSGCRISKDVPPYSIMAGnpv--eyhGVNAVVLQQHknts--ervLRHIANAYRLIYqg-------nfSLQDAV 227
gi 154494233 159 GTWSMTQTGCRFRKDVPPYIVAALept--tyyGVNSVILMHEgms---ekiVKHISHAYRIIYqg-------ntSIFDAL 226
                        250       260
                 ....*....|....*....|....*....
Feature 1                                     
1J2Z_A       229 KLEleehannpfVKEICSFIl--eSSRGV 255
2QIV_X       234 PEIaelaetypeVKAFTDFFa--rSTRGL 260
gi 30468068  227 EGIekin--ddyVRQVQRFMe--kSERGV 251
gi 88805697  227 QIIeaeveatpeRDEILQFIr--dSQRGI 253
gi 60491812  227 TKVeaevpaspeIEYIVDFIr--nSERGI 253
gi 89255946  231 EIIkamakedkvIEPFVDVIg--tSRRGI 257
gi 34539941  232 DIIqqeyadcheKELILDFIk--sSKRGI 258
gi 14285564  239 LKRnv------aVQYFSDFLfnsfRARGF 261
gi 60491183  228 QKIidqvpmseeIENIVAFVk--eSKRGI 254
gi 154494233 227 LMIkdqvpmsdeIQHIIDFIs--aSKLGI 253

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap