Conserved Protein Domain Family
LbH_LpxD

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cd03352: LbH_LpxD 
Click on image for an interactive view with Cn3D
UDP-3-O-acyl-glucosamine N-acyltransferase (LpxD): The enzyme catalyzes the transfer of 3-hydroxymyristic acid or 3-hydroxy-arachidic acid, depending on the organism, from the acyl carrier protein (ACP) to UDP-3-O-acyl-glucosamine to produce UDP-2,3-diacyl-GlcNAc. This constitutes the third step in the lipid A biosynthetic pathway in Gram-negative bacteria. LpxD is a homotrimer, with each subunit consisting of a novel combination of an N-terminal uridine-binding domain, a core lipid-binding left-handed parallel beta helix (LbH) domain, and a C-terminal alpha-helical extension. The LbH domain contains 9 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X).
Statistics
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PSSM-Id: 100043
View PSSM: cd03352
Aligned: 140 rows
Threshold Bit Score: 184.534
Threshold Setting Gi: 85375484
Created: 16-Aug-2001
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active LpxD is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Structure:2IU8_A; Chlamydia trachomatis LpxD binds UDP-GlcNAc and palmitic acid; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2IU8_A       136 TAIIE---DHVCIEPYAVVC-----QHAHVG---SACHIGSg-SVIGAYSTVGEHSYIHPRVVIRErVSIGKRVIIQPGA 203
gi 83858378  116 TATIA---DSARLAPGVIVG-----PDAVIG---ENARIEAg-AIIGPGVVIGDHARIGVRANVQC-ALVGARCEISAGA 182
gi 45644750   92 IFKHDseiKIPCIHKSATVS-----KDAEVH---KDVYIGPn-VFIGPNCKVHSGVEIHANCSLVRdVTVGSNSIIHHGT 162
gi 42557725   99 NMIYKk-kQLVGISSRASIS-----PTAKIG---RNCYIGDf-TVIGDNCEIGDDSIVYDRVSLVQnCTLGDACVIQQGV 168
gi 114569940 121 SAKIG---AGTRLGAGVVIG-----EGAEIG---TDCVIGPh-CVIGPGCRIGDRSRLSPHVSLQC-SDIGADCNILAGA 187
gi 114798725 119 LAQIH---ANAIVQPGAVIG-----PGAAVG---EGAVIGAn-AVIGPGVQIGRNTSIGANASIHC-ALVGDQVTILAGA 185
gi 150019519  81 FYLIH---NCLCRDINLYNKe---eFETIIG---DNCIIRDrkGISRKNVIIGNDVTIEENVIIREnVKILDNSVIRSGV 151
gi 119775386  71 FVLVDn-pRDLFAQALLVLKsenliSDVHPHfisPKAIISPt-ASIDFGVQIADDVVIEDFVSVKSgTIIGEGTLIRSFT 148
gi 85375484   84 SEIHA---RLAENHAQELRS-----VPNEID---SSARIDPsaHIADHGVTIGPNAWIGPHCAITPgVNVGEGCVLHSGT 152
gi 154151281  81 YEIHA---SLSRQTGGAFER-----LENTIH---SSSTIHPsaTIASRGVRIGRNVEIGKNVVIHEqTIIDDGVIIRSGS 149
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              ###                                                 #   #  ##         ##  
2IU8_A       204 VIGSCGFGYvtsafgqhkHLKHLGKVIIEDDVEIGANTTIDRGR--FKHSVVREGSKIDNLVQIAHQVEVGQHSMIVAQA 281
gi 83858378  183 VVGEAGFGLayen-gevfTLPHLGRVIIEDEATLGANATVDRGM--LKDTRIGKGARIDNLCHIAHNVDVGEYAVMAAFA 259
gi 45644750  163 ILGSEGFGYapsd-dgyvKIEQLGGLSLGKNVEIGANCTIDRGA--LDDTQIHDGVKLDNLVHIAHNVVLGKNSAIAASC 239
gi 42557725  169 TLGADGFAFerdtsgnleRFPHIMGVKIGKNVEISANSSVARGS--LSDTVIGDGSKLDALVHVAHNVKIGKYCELTAGT 246
gi 114569940 188 VIGEDGFGIavsn-gntvGILHLGSVLIGDHVTIGANCTIDRGL--FGATRIGASSKIDNLCHIAHNADIGENVIMAGYS 264
gi 114798725 186 RIGETGFGVlvgp-qgaeDSPHFGRVIIQDHVTIGANSCIDRGV--FEDTIIGERTKIDNLCQIAHNVVLGRGVIVAAFG 262
gi 150019519 152 ILGGEGFQFnkeg--rifFIEHCGGVEIGRNVEVQYNTCIDKAMfpWDNTVIGEETKIDNLVHVGHGAKIGKRCLIAANA 229
gi 119775386 149 CVGSEGFGIaksadgnniRFLHLGGVKIGKYCEIGLFNSINCGT--LSDTIIGDYVKTDAHVHIAHNCTIGNNSILTAAA 226
gi 85375484  153 ALGVPGFNTgiig-grlkIVPQMGGVRLGPHVEMLANCTVARGIf-GGHTSLGEETVADNLVYIAHDVQIGRRVQICALV 230
gi 154151281 150 VVGNTCTLKgkq---dciDMHPSGGVHIHRDVDIHANTIIDRAVf-KGYTVIGRQTKVDNLVHIGPGVRIGERCLVVACA 225
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1          ###                ##          #                           
2IU8_A       282 GIAGStkIGNHVIIGGQAGITGHICIADHVIMMAQTGVtkSITSPG-IYGGaPARPYQEIH 341
gi 83858378  260 GISGStvIGAGAQFGGRVGVADHLKIGQGARLAADAAVm-KDVPAGeTWAGsPAQPIQSFM 319
gi 45644750  240 AIAGSsvIGENFQMGGLSGVLGHLSICNDVTVGAHTLItkNIEKPGnYVGImPAQKHQDWA 300
gi 42557725  247 IIGGSttLGDMVWTGLNSMIKDNIKIGNNVIVAASAGVi-HDVVDGdIVAGvPAKSIRDKV 306
gi 114569940 265 GLAGSavIADNAMLGGRVGVYDHVTIGEGARVGANSAAs-RDVPAGeFWVGnPAQPMRQHM 324
gi 114798725 263 GISGSvrVGDGSMLGGRVGIADHVKVGDRVSLAASAGLf-RDVDSGeTWGGtPAKPIRQWM 322
gi 150019519 230 LIGGSsiIGNDCWIGVSVTISNGLIVGNNVSIKIGAVVt-TNIADGsAVSGnFAIEHSKFI 289
gi 119775386 227 VLSGGvsIGDNVWLGPNSSIIQKTSIGSDSLIGIGAVVt-KNIDSNvIAAGnPSKILRSNK 286
gi 85375484  231 NVLGRtiVGDEAYLGPSCVVKNGLVLGARARVNIGAVVt-TDLAEDaIVSGnFAVPHDRFL 290
gi 154151281 226 NIGDCvvIGNDSWIGPNSTLAEQILIGNQAYITLGSHVs-RDVGDNmLVKDkYVIDRQRFK 285

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