Conserved Protein Domain Family
LbH_GlmU_C

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cd03353: LbH_GlmU_C 
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N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU), C-terminal left-handed beta-helix (LbH) acetyltransferase domain: GlmU is also known as UDP-N-acetylglucosamine pyrophosphorylase. It is a bifunctional bacterial enzyme that catalyzes two consecutive steps in the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), an important precursor in bacterial cell wall formation. The two enzymatic activities, uridyltransferase and acetyltransferase, are carried out by two independent domains. The C-terminal LbH domain possesses the acetyltransferase activity. It catalyzes the CoA-dependent acetylation of GlcN-1-phosphate to GlcNAc-1-phosphate. The LbH domain contains 10 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X. The acetyltransferase active site is located at the interface between two subunits of the active LbH trimer.
Statistics
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PSSM-Id: 100044
View PSSM: cd03353
Aligned: 111 rows
Threshold Bit Score: 246.563
Threshold Setting Gi: 81624390
Created: 16-Aug-2001
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active GlmU is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Comment:The structure evidences show only the interaction of CoA and substrate to one interacting LbH subunit/surface.
  • Structure:2OI7_A; Escherichia coli GlmU binds Desulpho-CoA and GlcNAc-1-PO4; defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:2OI6_A; Escherichia coli GlmU binds CoA and GlcN-1-PO4; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1HV9_A       251 GVMLRDPARFDLRG--------TLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNs-VIGDDCEISpyTVVEDANLA 321
2OI7_A       251 GVMLRDPARFDLRG--------TLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNs-VIGDDCEISpyTVVEDANLA 321
gi 42409775  227 RNDLIKAEFYFQENkr----kiFTDSGVTLVAPETVFFSLDTQIARDSVIYPYVFFGTgvKIESGAKILpfSHLENCLIK 302
gi 81557250  226 RSELARAEATLQTRlr----naAMDAGVTLVAPETVFFSTDTVIEADVTIEPNVFFGPgvKVRSGALIRafSHLEGCEVG 301
gi 81558395  228 RADLAAADAIFQTRar----aeLLDLGVTLMAPETVYLAADTVIGRDTVIEPNVVFGPgvTVESGATIRafSHLEGCHVS 303
gi 81599209  221 RADLAIAESYFQCMkr----asFLQSGVTLTSPDQVFFSIDTQIAQDVIVHPYVVFGAgvAVEPGAEILsySHLEFCHIK 296
gi 81621047  239 QAELAAAEAVWQQNrr----kaLMVDGVTMPAPDTVHLAWDTQIAGGAVVEQFVVFGPgvSVASGAVIKafSHLEGAVVG 314
gi 109892109 226 GVNARSELAVAEALvqarlreaAMDNGATLIDPATVWFSWDTKIGRDVTIWPHVVFGPgvTVGDNVEIKgfCHFEGCMVE 305
gi 109892119 228 RSELAAAEAVIQGRlr----ekAMEGGATLTAPETVFFSADTRLGRDVSIGPFVTFGPgvEIGDGVEIKgfCHIEGARVA 303
gi 84704114  249 RAELAAAEATFQRRlr----leMMLAGVTLEDPDSTYFAFDTQIAEDVTVAPHVVFGPgvRIAANVRIEafSHIEGAVIE 324
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #               # #           #  #          ####    ###    #         
1HV9_A       322 AaCTIGPFARLRPGAELLeGAHVGNFVEMKKARLGkGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGaNKFKTIIGDDV 401
2OI7_A       322 AaCTIGPFARLRPGAELLeGAHVGNFVEMKKARLGkGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGaNKFKTIIGDDV 401
gi 42409775  303 SnAEVGPFTRIRGNTTIGnKAKIGNFVEVKTSEVGqNTRIKHLSYIGNAKVGQESNIGAGTIVCNYDGkNKHETNIGSNC 382
gi 81557250  302 EnAMIGPYARLRPGTLCAaQTHVGNFVELKNVELGeGAKANHLTYLGDASIGSGTNVGAGTITCNYDGvFKHRTTIGERV 381
gi 81558395  304 RgAVVGPYARLRPGAELAeNARIGNFVEIKNAEIGeGAKVNHLTYIGDASIGAGSNIGAGTITCNYDGvMKHRTTIGANV 383
gi 81599209  297 KgAIVGPFARVRGNSTIDrGCVVGNFVEIKESSLGeMSKVKHLSYLGNSTIGKNTNVGAGTVICNYDGrNKQHSDIGNNC 376
gi 81621047  315 EgALIGPYARLRPGAEIGpDAHIGNFVEVKKVKVGaGAKANHLSYLGDGSVGEKANIGAGTIFCNYDGfEKFETHVGKGA 394
gi 109892109 306 AgVAAGPFTRLRPGAEIGeGAHIGNFVEVKKATVEaGAKINHLAYVGDARVGAGANVGAGTITCNYDGfNKSFTDIGAGA 385
gi 109892119 304 AkATLGPYARLRPGATIAeGAHVGNFVEIKNSAVEqGAKVNHLTYIGDARVGARANIGAGTITCNYDGfGKYHTDIGEGA 383
gi 84704114  325 EgAVVGPFARLRPGTSVGaAARVGNFVETKNTSLGrGAKASHLTYLGDAEIGAGSNIGAGTITCNYDGyEKHRTLIGENC 404
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
Feature 1        # ##                ##                #   
1HV9_A       402 FVGSDTQLVAPVTVGKgATIAAGTTVTRNVGEnALAISRVPQ 443
2OI7_A       402 FVGSDTQLVAPVTVGKgATIAAGTTVTRNVGEnALAISRVPQ 443
gi 42409775  383 FVGANSSLIAPLNIHDeSVIAAGSVIVEDVPEkSLAIAREKQ 424
gi 81557250  382 FVGSDSILVAPVTVGDdALIAAGSVITSDVPPgDLALGRARQ 423
gi 81558395  384 FIGSNTMLVAPVTLGDgAMTATGTVVTRDVEPdALAKARVKQ 425
gi 81599209  377 FVGANSTIVSPIKVGDnAAIAAGSVITEDLPPrSLGIARSRQ 418
gi 81621047  395 FIGSNSALVAPVRVGDgAMTGSGSVITKDVEDgALALSRADQ 436
gi 109892109 386 FIGSNTSLVAPVKVGDgAVVGAGSVITKEVTPgALAVARGSQ 427
gi 109892119 384 FIGSNTALVAPVSIGAgAIIGAGSTIARDVEAdALALTRGPH 425
gi 84704114  405 FVGSNSSLVAPVTIGAgAYIGSGSVITKTVEPdSLAVARGRQ 446

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