Conserved Protein Domain Family
GT1_Gtf_like

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cd03784: GT1_Gtf_like 
Click on image for an interactive view with Cn3D
This family includes the Gtfs, a group of homologous glycosyltransferases involved in the final stages of the biosynthesis of antibiotics vancomycin and related chloroeremomycin. Gtfs transfer sugar moieties from an activated NDP-sugar donor to the oxidatively cross-linked heptapeptide core of vancomycin group antibiotics. The core structure is important for the bioactivity of the antibiotics.
Statistics
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PSSM-Id: 99960
View PSSM: cd03784
Aligned: 153 rows
Threshold Bit Score: 104.752
Threshold Setting Gi: 158431183
Created: 8-Nov-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1PN3: Amycolatopsis orientalis TDP-epi-vancosaminyltransferase GtfA binds desvancosaminyl vancomycin (DVV) and TDP. Contacts at 3.5A.
    View structure with Cn3D
  • Structure:1RRV: Amycolatopsis orentalis TDP-epi-vancosaminyltransferase GtfD binds vancomycin and TDP. Contacts at 3.5A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   ## #                                                                 
1PN3_A         1 MRVLITGcgsrGDTEPLVALAARLreLGADARMCLpp--dyVERCAEVGVPMVPVgravragarep-------------- 64
gi 15077455    1 MRIMFTAsnwaGHYMCMVPLAWALraAGHEIRVACpp--sqERGVAATGLMPVPVldapdmmesarlafyvqamytppqs 78
gi 21726900    3 ARVLVATtpgdGHVNPMVPVAQEMvsRGHEVRWYTgk--afRSTVERTGARHEPMrdahdfggmpreeafpqh------- 73
gi 27753575   12 TRVLIATtpapGHVVSMLEVAGELarRGHEVRWYTgq--afQRQVERVGAHFEPMspeldfsgrsreeafpeh------- 82
gi 54024021    1 MRLLFSVtpafGHLFPVLPLARAAlaQGHQVGVLAsagmadTLAHELPGADHLPAgtmplefsleaqrrtgl-------- 72
gi 68146477    1 MRILVTTapspGHFFPLVPFAWALraRGHQVLVTMpe--qfIAEAGRSGLPAVASvppidlraamdsgegphps------ 72
gi 115374119   1 MRVLFTTtpatGHFHPLVPTAQALqqAGHEVAFAAae--sfRAQVEASGFLVFPAgvgfesldfdleravppp------- 71
gi 116621099   2 ARLLFTVwpfaGHVHPALAIGHALrlRGHEVAFYTge--avRSLLEAEGFRCFPFarvdekrvfalassefpyrss---- 75
gi 116621100   2 SRFLITCwpfvGHLYPQISVALALrdRGHEVAFYShe--stRAIVQGEGFKLFPFvqvderryerihaleakvppt---- 75
gi 134101682   1 MRVLFTTtpgrGHFFPMVPLAWALrcAGHQVVVAApp--qiAESVRAAGLEHVPVgggapdagryr-------------- 64
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1PN3_A        65 -----------------------gelppgaaevvTEVVAEWFDKVPaai-egCDAVVTTgllpAAVAVRSMAEkl-giPY 119
gi 15077455   79 gprplplhpftgqpmqslndfdagdlrdfwkksiDAVQRSYDNAVSfgdhwrPDLVVHDi---MAVEGALVAAl----RG 151
gi 21726900   74 ----------------agltgitgmiagfrdifiEPAADQMTDLLAlledfpADVLVTDe---TFFGAGFVSEr----TG 130
gi 27753575   83 ----------------aglsglanfkigvrdifyRTAPRQMDDLSKilerfpADCILADd---MCYGACFVGErt-giPV 142
gi 54024021   73 ------------------dvmqptpevigeifggVRLEAAFDEAMAaapgwrADTVIAEa---FDTVGPAVAAa----LQ 127
gi 68146477   73 ----------------qregardasvehigrgfgRLSELTVEGTVAvaerwvPDLVVSEp---TEHAGAVAAAhr-gvPL 132
gi 115374119  72 ----------------rldvpeqletvldffvdrLARPMAQDLGKVcqr-wrPDLIISEsi-eFAGPMVAERRgipyaTI 133
gi 116621099  76 -------------iwqqlryakqlqanfrewlldTVPQQVEDLDTIlth-wrPDVLICDl--aFWSPILVLGEt----RG 135
gi 116621100  76 --------------qpghqtisvsmtayrdmladSIPEQVADLMPIlen-wrPDVLVTDl--aLWGPIVVIWEm----TG 134
gi 134101682  65 -----------------------eftaepwavlaEHAVDATTDLVArw---cPDLVVCGy---AELAGPVAAAvh-evPA 114
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             #  ##   #                                 #                
1PN3_A       120 RYTVLSPdhlpseqsqaerdmYNQGADRLFGDAVNSHRASIglppve----------hlydygYTDQPWLAADPvlsplr 189
gi 15077455  152 VPSVYVSpgfig-----tvetEPGLDLVSADPLSCFEKYGVewgr---------------driQYAVDPSPDVAvpplgd 211
gi 21726900  131 IPVAWIAtsiyv----fssrdTAPLGLGLPPSSSRLGRLRNtvlkqltdrvvmrdlrrhadvvRDRVGLPRIRKgafeni 206
gi 27753575  143 AWLANSVyiwgs-----rdtaPLGRGLGPASSPLGRVRNALlrfvsdhvvm--rdmrqeadrvRALVGLDRLRSsameni 215
gi 54024021  128 LPWHQVGlgpalp---epivaQITGAAAKRYSERGLDPTPPrcyi---------------dpcPPAVQTPDLRRdvprlp 189
gi 68146477  133 LRHNWGLe-------------LPPETEEIAADQMAPTLARLglp----------------apaKAGLRVDICPEslqrpq 183
gi 115374119 134 QVGGMGSlgeasq---alfarRLDVLRAEQGLPPDPELKALfrylh-------------lsfmPAEYFGGPLPAttqylk 197
gi 116621099 136 IPVAVLSvlaacl---lpgpdAPAWGQGAPRPRNRIMRLRStmkrkllqwls-agflaqangiRRRYGLPALRCsvtefa 211
gi 116621100 135 IPVALSStmmgsm---ipgpdAPPWGPGLPPPRTSATRFLSritelgvdiva-hnlrrrvnqiRARYGLRPMGCsvtaft 210
gi 134101682 115 VSHHWGLhla--------desVRRLFSGTAAIRLRRLHARFglsd---------------eeaAPELVVDLCPPslarvd 171
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                     #                  
1PN3_A       190 ptdlgtvq--------------------tgawilpderplsAELEAFLAa-gSTPVYVGFGSssr-----------patA 237
gi 15077455  212 alrlp-------------------------mryvpyngaqgADPWQLGPi-rGRRVCIVWGNsasgifgadvpalrhaiD 265
gi 21726900  207 mrtpdlyllgtvpsfeyprgdmppevrfvgpfvspappdftPPAWWGELdsgRPVVHVTQGTvandaer----lllpaiR 282
gi 27753575  216 arppalyllgtvpsfefprsdllpgthfvgpllgvppehfdPPAWWEDLdgdRPVVLITQGTtandidg----llrpavR 291
gi 54024021  190 lrp-----------------------------qphaspgstWEPPTGQPg--RPRVLVTLGTifsdra-----vldevvR 233
gi 68146477  184 arpaqr------------------------mrfvpysgagvTPPWLLEEp-dRPRVCVTLGSvvpmihgv--kvlkelgE 236
gi 115374119 198 pev------------------------------fdqsgferLPEWMASLg-sRPVVYATLGTvfnklir----hlstitE 242
gi 116621099 212 grmplylvpsmreydyd-rrdlppsvhyvgpclwdkpsaapPPDWLLRLpagRPLIYATEATigtgepf----llqaaaR 286
gi 116621100 211 grlplylipslpeldyn-rqdqppnvhyigpcvwtkpvgapPPEWLNQLptdRPWVHATEGTaqyqepf----llrataQ 285
gi 134101682 172 rtgwlp------------------------mrhipyggagiPAPWLWEPr-dRPRICVSMGGv---------------pA 211
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                # # ##  #               
1PN3_A       238 DAAKMAIKAVRas-grRIVLSRGwadlvlpddgadCFVVgevNLQELFGrVAAAIHHDSaGTTLLAMRaGIPQIVVRrvv 316
gi 15077455  266 AAVRQGAEVVLta-pqEQVEELGalp-------agVRVLrncPLELILPyCDLLVHHGSaNCYMNGIVaGIPQLSLAlny 337
gi 21726900  283 ALAAEDVLVVAt---tGAPLELEpmp-------anVRVErfiPHHALLPhVDAMVTNGGyGGVNTALAhGVPLVVAAate 352
gi 27753575  292 ALADQEVLVVVttgsdLDVERLRplp-------anVRLErfiPYHHLLPrVDAMVTNGGyNGVNAALAqGVPLVVVPgse 364
gi 54024021  234 AVASTGATVIAti-gmAMREEQAagqqv--ahpesVHFVqfaPMEQLLSgIDLVVCAGGsGTVLAAMSrGIPLVIWPqga 310
gi 68146477  237 ELSGDDMETVIav-pdRFADELGalp-------pgVRAAawlPLGLLLThCTAVVHHGGiGTALTALAfGLPQVALPhmg 308
gi 115374119 243 ALREEPVDLIVtvgrdMDPARLGpqp-------snVHVEryiPQSLLLPrCELAILHGGyNSVMSALYvGLPVLIVPlaa 315
gi 116621099 287 AFRNLPVDVVMttgaqRNPAGLKlads-----apnVRVEsyvPQSDLLPrTAVMVTLGGsGGVLAALKaGVPLVVVPtew 361
gi 116621100 286 ALAGLPMEVILttgqnRDPATLGldpl-----panVRVErwlSHESLLPrCAAIVTTGGpATVMAALKaGVPLVMVPtfw 360
gi 134101682 212 EVVHETVRGLAga-daEIVLTGSdcpa----dlpgVRAGgrlPHDQLLPtCDAVVHHGGsGTAMNSLLcGLPQLVLPhag 286
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                                                   
1PN3_A       317 dnvveqayhADRVAELGVGVAVDgpv--ptiDSLSAALDTALA-PEIRARATTvadt--iradGTTVAAQLLFDA 386
gi 15077455  338 ------dtlICGRRIDPAGATLTlsgleataEKVEEALRRVLF-DHRYRRAAEklrd---gveRAPSPAAVAGLL 402
gi 21726900  353 e----khevAARVSWSGAGVHLKkrr--lseRDIRRAVRAVLD-EPRFRVHAArl------rdEYAARDAVVDAV 414
gi 27753575  365 e----kpdvAARVEWAGAGVVLErrp--vseADLRAAVTTVLR-DGSHRRRARalaeehrsvdAPRRAADLIESM 432
gi 54024021  311 d----qpinAARARAAGVALAVDt------aAALTQAVDTALH-DPALRAAAAtaar---qiaALPAPHDVLDEI 371
gi 68146477  309 ------dqhATADRLAAAGAAAVvrreditpAAVAEALRRVRS-EPGYAEGARalra---entAAPGLDEVACLA 373
gi 115374119 316 d----qpmnAQSCERLGVGRRINpdt--ltpELLRQQVREMMR-DGSYRERARrfqaesqtlpGMAEGVAMLEKL 383
gi 116621099 362 d----rpenAQRVVEAGAGIRIPpnr--ctpERLRSAVERILR-EPSYRANAQrlaavsahysGPDRASELLEGL 429
gi 116621100 361 e----kndnAQRVVEAGVGLRLAprr--cspDRVRAAVMRLLE-EPHFRENARriaqrlseasGARGAAQLLENL 428
gi 134101682 287 e----qfenAERLVAAGVARQVGsae--rdaGTLGAELEELLDdPSYRDRALRirs----eieSMPAPGEVVLAL 351

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