Conserved Protein Domain Family
GT1_UDP-GlcNAc_2-Epimerase

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cd03786: GT1_UDP-GlcNAc_2-Epimerase 
Click on image for an interactive view with Cn3D
Bacterial members of the UDP-N-Acetylglucosamine (GlcNAc) 2-Epimerase family are known to catalyze the reversible interconversion of UDP-GlcNAc and UDP-N-acetylmannosamine (UDP-ManNAc). The enzyme serves to produce an activated form of ManNAc residues (UDP-ManNAc) for use in the biosynthesis of a variety of cell surface polysaccharides; The mammalian enzyme is bifunctional, catalyzing both the inversion of stereochemistry at C-2 and the hydrolysis of the UDP-sugar linkage to generate free ManNAc. It also catalyzes the phosphorylation of ManNAc to generate ManNAc 6-phosphate, a precursor to salic acids. In mammals, sialic acids are found at the termini of oligosaccharides in a large variety of cell surface glycoconjugates and are key mediators of cell-cell recognition events. Mutations in human members of this family have been associated with Sialuria, a rare disease caused by the disorders of sialic acid metabolism. This family belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
Statistics
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PSSM-Id: 99962
View PSSM: cd03786
Aligned: 233 rows
Threshold Bit Score: 233.937
Threshold Setting Gi: 121611756
Created: 8-Nov-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitehomodimer
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #                                                                       
1F6D_A         2 KVLTVFGTRPEAIKXAPLVHALAkdp-ffEAKVCVTAQHReXLDQVLKLf------sIVPDYDLNIxqpgqglTEITCRI 74
gi 71895297   11 RVCVATCNRADYSKLAPIMFGIKaepqffELDVVVLGSHLiDDYGNTYRmieqddfdIHTRLHTIVrgedeaaMVESVGL 90
gi 86748660    5 KICVVVGSRANYSSIKSAMRAIQdhp-alELQLIVAASAVlDRYGSVVNliekdgfrPHARVTMLIegetpatMAKSTGL 83
gi 115637356  45 RVCVATTNRADYSKLGNIMQAIKdde-mlELSTIVLGCHLiDDYGSTYRliekdgftIDSRLHTIVrgedeaaMVESVGL 123
gi 117923875   2 KLLGITCSRAEYDLMSGLYMRLHadp-dvELKLLVAGSHLsPTFGRSVQairqdgldILLTLETLLdadsrvsRLKSASL 80
gi 118072069   2 KILAITSIRSDYDLLSPLFFKLQqdt-sfVLKLLVGGAHNsTSFGHTVNdikndgfdILMEIESLLdsnssagRLKSASI 80
gi 118404390  11 RVCVATCNRADYSKLAPIMFGIKaepdrfVLSVVVIGSHLiDDYGNTYRmieqddfdIQARLHTIVrgedeasMVESVGL 90
gi 153955088   5 KILAFTGIRSDYELMSLLYKKLNean-dfEIKLVVSGAHLsETYGYTLNnivedgipVIAQIENLIdsnsrasRIKSLSI 83
gi 161322916   5 KICAVVTARPSYSRIKTALKAIQehp-nlELQLVVAGSALlGRYGNAVNviekdgfkIDEKVFMVLegenptaMAKTTGL 83
gi 161403559   4 KLCLVITTRGNYAKVKSIIQAAEndp-dvELQIIVGGGAIlAKYGNIADslttmgvnVDRRIHFLVegetpvtMAKSAGL 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1F6D_A        75 LEGLKPILAEf-kPDVVLVHGDTtTTLATSLAAFYqRIPVGHVEAGLrtgdlySPWPEEANRTLTGhlaXYHFSPteTSR 153
gi 71895297   91 ALVKLPDVLNrlkPDIMIVHGDRfDALALATSAALmNIRILHIEGGEvs---gTIDDSIRHAITKLa--HYHVCCtrSAE 165
gi 86748660   84 GLIELPTLFEqlgPDVVLTVGDRfETMATTLAAAYmNIPVAHTMGGEvs---gTIDESIRHAVTKFa--HIHFPAsqGAA 158
gi 115637356 124 AMVKLPDIMLrlkPDVVIVHGDRfDVLSLAACAALmNIRIVHVEGGEvs---gTIDDSIRHTISKLa--HYHVCCteRAH 198
gi 117923875  81 LLQNSIDLVAhydPDLILYAGDReEVIIGGLLGAYlEIPTCHFFGGDhsq-dgHVDHMVRHATSKLs--TVHMVSlpQHA 157
gi 118072069  81 LLISAIDVVKsfnPDLIIYAGDReEVMVGALLGGYlSIPTIHFYGGDhaa-dgHIDNPIRHAVSKLs--TCHFVStdEHV 157
gi 118404390  91 ALVKLPDVLNrlnPDIIVVHGDRfDALALATSAALmNIRILHIEGGEvs---gTIDDSIRHSITKLa--HYHACCtrSAE 165
gi 153955088  84 LLQDCIHTAAaykPDVILYAGDReEVVVGGLIGTYlRIPTIHFFGGDhal-dgNIDNLVRHAVSKLs--SLHFVSneESK 160
gi 161322916  84 GVMELSNVFYnlkPDAVITIADRfETIATSIAAAYqNIPLVHIQGGEvt---gNIDEKVRHANTKLa--DLHLVAseDAK 158
gi 161403559  83 AVSEFTTAFEnlqPDVVMVIADRfECLSIAMTASYmNIPVAHMEGGEvs---gSIDESIRHAITKLs--HVHFPAtqEAA 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1F6D_A       154 QNLLREnVADSRIFITGNTVIDALLWVRDqvxssdklrselaanypfidpdkKXILVTGHRREsf----gRGFEEICHAL 229
gi 71895297  166 QHLIAMcEDHDRILLVGCPSYDKLLSAKNkdymsvirm-----wlgedvkprDYIVALQHPVTtdikhsiKMFELTLDAL 240
gi 86748660  159 ERIIKLgELPRHVHMVGCPRIDLVAEILGrssggldaglfdlgvgqqfsvdePFALVSQHPVTteygtgeAQITLTLEAV 238
gi 115637356 199 KRLLAMcEDNDRILLAGCPSYDKLLSTDVvdcqhilnr-----wlkgegkskEYIVALQHPVTtniadslKMYSLMVDAL 273
gi 117923875 158 QRLLKMgEAPQRVHQIGSIALDKFVRHQPaelaaihaq------lgiqegftRYALVIFHPVAeekaqahHYFENILLSL 231
gi 118072069 158 RRLLSLgEPPQRIFNIGSIALDKFNNVKPipnikdli--------anktvtkPAAILIYHPIDeekristQLIDTMVESL 229
gi 118404390 166 QHLIAMcEDHDRILLAGCPSYDKLLSVNNkdymsvikm-----wlgddaksgEYIVALQHPVTtdikhsiKMFEFTLDAL 240
gi 153955088 161 QRLIKIgENKNRIFNVGSPALDKFISTEYigkkeivh-------ilgkprwnNYAVMIFHPLAgeedragKYFEEILVAL 233
gi 161322916 159 DRVVKLgEDPAYVINTGCPSIDIAKEVLEnnnyefdpikkyggvgadldisnGYLVVMQHPETnryksarEDVLCTLEAV 238
gi 161403559 158 DRIIRMgEAPETVFPVGATSLDVIAGLDLdnlqpimekqasagvgarvdltqPYLTVIQHPVTteyaenlEHVNQTLNAI 237
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                      # #  #                #   #       
1F6D_A       230 ADIAtthqdiQIVYPVHln--pNVREPVNrilg---hvknVILIDPQEYlPFVWLXnhAWLILTDSG-GIQEEAPSlgKP 303
gi 71895297  241 ISFNkr---tLVLFPNVdagskEMVRVMRkkgie-hhpnfRAVKHVPFDqFIQLVAh-AGCMIGNSScGVREVGAFg-TP 314
gi 86748660  239 REQGla---aIVLWPNAdagsdDISRGIRkwrerklddrmHFFKNLPIEtYVNLMRs-AACLVGNSSsGIREGAYIg-TP 313
gi 115637356 274 MEFNkr---vIMLFPNIdagskDIVRIMRqkgve-tnpmfYPVKHIPFEeFIILVAn-AGCMIGNSSaGVREAGAFg-TP 347
gi 117923875 232 KKRNip---aLVSAPNAdegfsRLLEVVEhyrg---dphfHFYRNLERAlFLSVYKr-CDFLIGNSSsGIIEAASIp-IP 303
gi 118072069 230 NSAGyh---cFLGLPNSdpgnaEIRSALKhaakk--yedvTLYGNLSREqFVNLFKq-SSLIVGNSSaGLLEAASIp-VP 302
gi 118404390 241 LSFNkk---tLILFPNIdagskEMVRVMRkkgve-hhpnfRAVKHVPFEqFIQLVAh-AGCMIGNSScGVREAGAFg-TP 314
gi 153955088 234 EKKSvn---aFVSYPNVdsgnkKIIEVIKkyyn---kedfKFYKNIPRTlFVNLLRn-SMFMIGNSSaGLYEAPILk-LG 305
gi 161322916 239 HELNip---tFWFWPNVdagsdGTSNGIRyyrenfnpeniRFFKNMEPNdFLHLLKn-CDCLVGNSSvGIRECSFIg-VP 313
gi 161403559 238 HELGmg---tIWIWPNMdagsdGISKGIRifrekhvdapmHFFKSLPIEhYAPLLKn-AQAIVGNSSsGIREAAFLg-TP 312
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                                                                           
1F6D_A       304 VLVXRdtterpeavtaGTVRLVgtdkqRIVEEVTRLLkdeneyqaxsrahnpygdgqaCSRILEALK 370
gi 71895297  315 VINLGtrqtgretgenVLHVRDadtqdKILHALQLQFgkqypcsk------iygdgnaVPRILKFLK 375
gi 86748660  314 VVNIGtrqhmrdrgdnVIDVGYdk--kQISDAIARQVehgryamd-----piygdgtaGTKIADILV 373
gi 115637356 348 VVNLGsrqtgretgenVLHCRDadttqKIHHALEIQYqrqfppsy------iygdghaVPRIIKFLK 408
gi 117923875 304 VINVGlrqrgrlaganVHFCDPhp--eALDHALEQVAkpafqnslkg-lknpygdgqsEVKAHQLIR 367
gi 118072069 303 CVNVGarqrdryctdnVLFVDAtl--kAIKQGIKQASspeftkklaq-isnpygngnsAKLAYSLIK 366
gi 118404390 315 VINLGtrqtgretgenVLHVRDadtqnKIIHALQLQFgkrypcsk------iygdgnaVPRIVKFLK 375
gi 153955088 306 AVNVGnrqkgrlcaenMIFVHQgv--sNIVKGIETVLteefkllldk-vqspygsgnsVEKIISLMR 369
gi 161322916 314 TVNIGtrqnrrqrgknVVDAEYnk--eAIKTAIQERIgqkgmlse-----hiygngnsGKQIADALA 373
gi 161403559 313 CVNIGsrqsgrdrsvnIIDVGYdk--aEIKAAIQKQLdhgryeen-----hmwgdgkaGPKILDVLK 372

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