Conserved Protein Domain Family
GT1_TPS

?
cd03788: GT1_TPS 
Click on image for an interactive view with Cn3D
Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
Statistics
?
PSSM-Id: 99963
View PSSM: cd03788
Aligned: 124 rows
Threshold Bit Score: 311.779
Threshold Setting Gi: 84872458
Created: 8-Nov-2005
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
active sitehomotetramer
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1GZ5: E.coli TPS binds UDP and glucose-6-phosphate. Contact at 3.5A.
    View structure with Cn3D
  • Structure:1UQT: E.coli TPS binds uridine-5'-diphosphate-2-deoxy-2-fluoro-alpha-D-glucose (U2F), a non-transferable analogue of UDP-glucose. Contact at 3.5A
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                    ##                                                  
1GZ5_A         2 RLVVVSNRIAPPdeh---------aaSAGGLAVGILGALKAa-----gGLWFGWSgetgnedqp---------------- 51
gi 84872458    5 EIFLASKRAAITydtdpatgeprawlAPGGTGNVVAEQAGVl-----nISWIASAdseddrrasalnp------------ 67
gi 29830479    8 QILVASNRGPVSytldetg-tltskrGGGGLVSGLSAIGPDa-----gALWVCSAlgegdrea----------------- 64
gi 108805757   6 GVVIVSNRAPVTfsrsgsg-erthsrGAGGLVTALNAVSRRae----gAVWIASAasdedvrvare-------------- 66
gi 115377410   5 RIFLASKRAPVTyhrdvsg-altaelAPGGTANVVADLASQl-----gVSWIACAmtdedrhvasqhp------------ 66
gi 158312043  36 DLLIASNRGPVSfvvgddg-llhprrGGGGLVSGLAQVVQDtpedgreIVWVCAAlsdadrraarsapg----------- 103
gi 2622886    16 NPVIVSNRGPVEffkend--etvmkrGAGGLVSTLLPLVERf-----sGVWISSAmtvedarvasgyp------------ 76
gi 117929160  14 PVIVASHRAPVQfrregg--rriarrGAGGLVTALLGLVREip----dAVWVCAAsteedaavvaehrgkaipiavgplp 87
gi 158316749  10 PLVLLSNRGPVSfdrdas--grtvnrGAGGLVSALSGLAEHld----dAVWVCGAatpedaavagendggailv-qtqpt 82
gi 125491439   7 AVLVATKPAPVVhervde---rvvprLSVSTGSVVGDMASRl-----rTTVVASAaseedralaersp------------ 66
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                    #                                                   
1GZ5_A        52 ----lkkvkkgniTWASfnlsEQDLDEYYNQFsnaVLWPAFHYrldl-------vqfQRPAWDGYLRVNALLADKLLPll 120
gi 84872458   68 dgvtmelhsgreiLVRLirhdPAVFRNVQNFMtanLMWAANNYgwdrwtq-psfgsdAREGWADFGRFTRDFADAILKss 146
gi 29830479   65 -----vrrgvgepGVRMldvdAEVHADAYNGIansVLWFVHHMlyqtple-pvfdaeFRRQWAAYETYNHAFAAALAEea 138
gi 108805757  67 ---segpcevddlRLVLvehdAGAYDLMYNRLanpLLWFVQHGlydlpyapelgeetLRAWEEGYLPVNRSFARAAAEai 143
gi 115377410  67 rgmevkvsaaapvLLHLlqhdPAVFEDMQEIItadLLWASNNYlwdgwtk-ptfdtrSHRAWKHFETFTETFATTLLErs 145
gi 158312043 104 gridrsgydtggaSVRMldldRVLFDRAYNAVanrTLWFVLHLlhapate-psfgasFRKDWAAYEAYNAAFADALAEla 182
gi 2622886    77 enrvplpeddprfTVSFvivdRERYESYYSIIsnpLLWFVQHYmwntpydpqiddkvYRAWEDGYVHVNKKFGDKVISei 156
gi 117929160  88 riadpdahdepvaAIRMveidPAAREDFYSVIsnpMLWFVQHGlydltrapnideveHEAFEHGYVAVNRAFADTIAEev 167
gi 158316749  83 phvvegqpdgpsiRVRLvvtdPDAYADFYSVWsnpILWFIQHGlygrslapvltraeHDAFENGYAAVNRAYADAVADev 162
gi 125491439  67 dgvtvraaggqeiRVRLvrhaPETLSTERYGLtteVLWHTMHNlwdrwsa-psfdraSRRAWESLHALNQDITEALLAqs 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    # #                      #                                          
1GZ5_A       121 q---ddDIIWIHDYHllPFAHELRKrgv-nnRIGFFLHIPFPTPEI--FNALPTYDTLLEQLcd--yDLLGFQtENDRLA 192
gi 84872458  147 aq-sadPVYLVHDYQlvGVPALLREqrp-daPILLFVHIPWPSADYwrILPKEIRTGILHGMlp--aTTIGFFaDRWCRN 222
gi 29830479  139 a---egAAVLVQDYHltLVPRMLRElrp-dlRIGHFSHTPWAPSEY--FRMLPDDIAAQVLGgilgaDRAAFLtQRWADA 212
gi 108805757 144 sg-aeePVVLLHDYHlyMVPELLREevgegpLISFFVHIPWPEPDL--WRVLPSYIREGALRsllrsDIVAFHtRRYARN 220
gi 115377410 146 ka-cpnPVYLLHDYQmcGVPQHLRAqrp-eaPQLLFVHIPWPSADYwrMLPRPMREGLLRGMlg--aDVIAFFaTRWVRN 221
gi 158312043 183 a---pgACVLVQDYHltLVPARLRQmrp-dlRIAHFSHTPWSPPEY--FRMLPDDVSAAILHgmlgaDRLGFLaPAWAAA 256
gi 2622886   157 ernekdSLIMLQDYHlyLCPFFIKKeig-dvFLSHFIHIPWPQRDY--FNILPERMRADIIGgllsnTILGFHiERYCAN 233
gi 117929160 168 esrggrALVMLHDYHfyLVAAMVRErcp-dvVLSHFVHIPWPGPDA--WRVLPPHMRDQIFAgllgnDIVAFHtERFARN 244
gi 158316749 163 qsrggkALVLLQDYHfyLVAEHVREqcp-dvVLTHFVHIPWPGPDE--WRVLPGNIRERLLRgllgsDVVGFHtRRYARN 239
gi 125491439 146 p--gagSGYLIHDYQlgMVPARLRRadp-taRVLFFHHIAWPGPEGlaLLPRQFADDLLRGMlg--aDVVCFFaRRWCRN 220
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                  #    #
1GZ5_A       193 FLDCLSnltrvttrs--akshtawgkAFRTEVYpigiepKEIAKQAagplppkla---qlkaelknvQNIFSVERLDYSK 267
gi 84872458  223 FLESVAdllpdaridreamtvewrghRTRLRTMplgyspLTLDGRNpqlpegi-------eewadghRLVVHSGRTDPIK 295
gi 29830479  213 FTDCCHavlgp-----------gipsGTRIGVHglgadaDFLRERAhrpdveerma-alreevgegrKTIVRVDRTELSK 280
gi 108805757 221 FMAAASeilgaeadpa-rgtlrrggrEVWVRSYpisidpAEFEEVAespevrree----eyvrnlpgALLVRVDRTDLSK 295
gi 115377410 222 FLACVEdllpearvdvpsatvrwegrAVRVEAMalgyspSALDIREgevspel-------aawigdhPLVVHSGRTDPIK 294
gi 158312043 257 FRDCCAeilgaevvg---dtvtaggrTVRTSLYplgvdaAELRDRAarpdvraraa--elrelaggcRLVVRVDRTELSK 331
gi 2622886   234 FFECCEdlgyevdgd--rgivrngdeKTLVKSYpisvdpESMERTVssptfmekde--fvrklkgnmFLIYRTDRADLSK 309
gi 117929160 245 FLLCAQellglpinld-kmtvqygprTIAAKTYpisidvDALEEVArskavrtrlddletwlaaeggKLIVRVDRTDPSK 323
gi 158316749 240 FVLCAGellglpinld-dltvtvnnrVVSARYYpisvdpAALDATLasedvaahmamlryvfldedrQLVLRVDRTDPSK 318
gi 125491439 221 FLRCVEdlvpgarvdhdagtvrlggrEVRVAAEplsyspDALKGLPrswpkel-------dawvgerPLVVHSGRTDPIK 293
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                    #   
1GZ5_A       268 GlPERFLAYEALLEkypqhhgkIRYTQIAPtsrgdvqaYQDIRHQLENEAGRINGkygqlgwtPLYYLNqhfdrKLLXKI 347
gi 84872458  296 NaERAVRAFVLAARggg--lekTRMLVRMNpnrlyvpaNADYVHRVETAVAEANAelg---sdTVRIDNdn-dvNHTIAC 369
gi 29830479  281 NiVRGLLAYRELLAsrpdwrerVVHVAFAYpsrqdlavYRDYTAAVQRLADEINAeygtpgwtPVVLHVkd-dfARSLAA 359
gi 108805757 296 NiVRGFRAYGRMLElhpemrgrVTFFAQLQpsrgdipeYAAYAEAIRRSAEEVNEahgtpswrPVELSMqd-nfPRSVAA 374
gi 115377410 295 NaERAVLAFTAALQedpg-lrhARLLVKMNpnrlsveaNQQYRERVGRAVEAANRslg---teTVRLVCtn-svNGTFAA 369
gi 158312043 332 NiVRGLDAYRELLRshpewhgrVMHLVCAYpsrhdlpeYREYTAAVQRLAAEIEDefgtdtwlPVHLEInd-dfPRSLAA 410
gi 2622886   310 NiIRGFRAYELFLRdhpefhgkVKFLATGKptrqqireYRNYSEEVKDTVEEINEkygtdewkPIEYIYra-dyELVAAA 388
gi 117929160 324 NiVRGFLAFERLLDdhpelvgkVRFLAFLQpsrldvpeYVEYIGRIGSVVARINAkytrggeaPIDLRLhe-dfLTAVAA 402
gi 158316749 319 NvVRGFLAFGTLLDqhpelvgrVSFLALLQpsrtdvpeYADYIALIGAVVAEINAkhtkegrqPIDLRMve-dfALAVAA 397
gi 125491439 294 NaHRAVAAFRIAAEhpa--aadARLLLRINphrlhigaNAAYLRMTEEAVAEANRhfg---eeRVRLLTgn-drGLTFGA 367
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         ##  #                                                          
1GZ5_A       348 FRYsDVGLVTPLRDGXNLVAKEYVAAQDpanPGVLVLSQFAGAane-ltSALIVNPYd--rDEVAAALDRALTxslaERI 424
gi 84872458  370 FRRaDLLIFNSTVDGQNLSTFEAPLVNEr--DADVILSETCGAaevlgeYCRSVNPFd--lVEQAEAISAALAagprQRA 445
gi 29830479  360 YRLaDVALVNPIRDGMNLVAKEVPVVSDe--GCALVLSREAGAyeelgeDAITVNPYd--vVGTAVALHEALSmpagERA 435
gi 108805757 375 YKNyDALLVNAVRDGMNLVAKEAAVVNRr--DGALILSENAGAheelgeHALTVNPFd--lDDQAAAIHRALTmppeERA 450
gi 115377410 370 LRRaDVLLLNSTVDGQNLTAFEGTLFNQr--DAVLILSERCGAaealasVSRLVNPFd--iVEQAQALREALRaspaERA 445
gi 158312043 411 MTLaDVLVVNPIRDGMNLVAKEGMVVSAd--GGVLVLSREAGAcsqmgaDAVVVNPFd--vTATAEAMHTALVmpaaERR 486
gi 2622886   389 FKNyDCLIVNPIADGMNIVPKEAAIINRe--DGALILSERAGCydelaeHVIGVNPFd--iKETADAIYSAMKmspgKRK 464
gi 117929160 403 YSIcDVVMVNSIADGMNLVAKEAVCVSRr--NAVLALSENTGAye--elGAFAVTLYpfdiQQQADALYIALMmpeeERR 478
gi 158316749 398 YSIcDVLMVNAIADGMNLVAKEVAVVNRr--GGVLALSEMAGSheelgeFAVTLHPFd--vQQMADALYTALTmpmeERH 473
gi 125491439 368 LARaDAVVLNSTADGQNLTAFEAIAMGAr--EPVLVVSHTCGAgevlgdGALRVHPHd--lVDQADALVEALTmtpsRRT 443
                        490       500
                 ....*....|....*....|....*..
Feature 1                                   
1GZ5_A       425 SRHAEXLDVIVKNDINHWqECFISDLK 451
gi 84872458  446 EAAARRRDAARPWTLEAWvQAQLDGLA 472
gi 29830479  436 ERTKRLAGAATALPPARWfLDQLHALD 462
gi 108805757 451 RRARALRETVLSNTIEDWaAAQMRDIA 477
gi 115377410 446 EAARRRREVVLQYDLPTWvRHQLSSLG 472
gi 158312043 487 ERARRLAAVAGELPPRAWfRAQLDEIR 513
gi 2622886   465 KLSEGLKSRVLKRTIYHWiNEQFDDFE 491
gi 117929160 479 ERLEAAAEIVRANNVTKWlDFQLTDLA 505
gi 158316749 474 RRLAAAAEQVRTHDVRRWlNDQLADLR 500
gi 125491439 444 AASRVLHEAAARFALPHWtERQLGLLG 470

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap