Conserved Protein Domain Family
RJL

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cd04119: RJL 
Rab GTPase family J-like (RabJ-like)
RJLs are found in many protists and as chimeras with C-terminal DNAJ domains in deuterostome metazoa. They are not found in plants, fungi, and protostome metazoa, suggesting a horizontal gene transfer between protists and deuterostome metazoa. RJLs lack any known membrane targeting signal and contain a degenerate phosphate/magnesium-binding 3 (PM3) motif, suggesting an impaired ability to hydrolyze GTP. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization.
Statistics
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PSSM-Id: 133319
View PSSM: cd04119
Aligned: 13 rows
Threshold Bit Score: 239.565
Threshold Setting Gi: 74831325
Created: 27-Aug-2005
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:Based on sequence similarity with other Rab isoforms.
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #######         #      ##                                               
gi 56789664  17 IKVISMGNAEVGKSCIIKRYCEKRFVp-KYQATIGIDYGVTKVQik-----------------------------drEIK 66
gi 32997031  24 IKIISIGSSGVGKSCVIKRYCEGRFVs-KYIPTIGIDYGVKRVSlrapahm-------------------spappnfFVR 83
gi 42475926  17 IKVISMGNAEVGKSCIIKRYCEKRFVp-KYLATIGIDYGVTKVQvr-----------------------------drEIK 66
gi 42491777  17 VKVISLGNAEVGKSCIIKRYCEKRFVp-KYLATIGIDYGVTKVQvr-----------------------------drEIK 66
gi 21706512  17 IKVISMGNAEVGKSCIIKRYCEKRFVs-KYLATIGIDYGVTKVHvr-----------------------------drEIK 66
gi 42491781  39 VKIISMGESGVGKSCVIKRYCEEKFVs-KYISTIGIDYGVKPVMvn-----------------------------gaEVR 88
gi 42475924  13 VKIISMGNAEVGKSCIIKRYCERRFVs-KYLPTIGIDYGVTKVSvk-----------------------------dkEVK 62
gi 70834739  22 IKIVSLGSVGVGKSCLIKQYCEGRFVs-KYIPTIGIDYGVKRVDvrvpahl--------------------apsgkiSTR 80
gi 42491767  39 IKVLTIGNTKVGKSCIVKKYCEKNRFvsNYNATIGVDYGVKSVSrtmdd------------------------gsnlNIK 94
gi 19387544  15 LKVTTAGMSQVGKSCLIKYYCEGRFVk-KYLPTIGVDYGVRALDle-----------------------------gcTVR 64
gi 74831325   8 IKVISVGDTQSGKSCLIKRFCEGRFIn-KYVTTIGVDYGVKKMMiq-----------------------------nkKVA 57
gi 45478200 111 HMHTVKIELKHFKSCIIKRYCEKRFVs-KYLATIGIDYGVTKNTnvdldly-------------------kdhligkLND 170
gi 68124554  72 IKLLVIGDVGVGKSCLIKRYCEGRFVa-KYIPTIGIDFGVKKVEvskaavlqqrssepsppaataqsgggassaippAVR 150
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #                                                                        
gi 56789664  67 VNIFDMAGh--PFFY-EVRNEFYKDTQGVILVYDVGQKESFESLDAWLAEMKQElgpqin-------------------- 123
gi 32997031  84 VNFWDMAGr--DEFL-EIRNEFYYAVEGVLLFYDVTDASSFAALDEWLKEMQTYvnaprstgge---------------- 144
gi 42475926  67 VNIFDMAGh--PFFY-EVRNEFYKDTQGVILVYDVGQKESFDALDAWLAEMKQElgphg--------------------- 122
gi 42491777  67 VNIFDMAGh--PFFY-EVRNEFYKDSQGVILVYDVGLRESFDALDSWLTEMKQEmgsqa--------------------- 122
gi 21706512  67 VNIFDMAGh--PFFY-EVRNEFYKDTQGVILVYDVGQKDSFDALDAWLAEMKQElgphg--------------------- 122
gi 42491781  89 VNFWDLSGl--PEYL-EVRNEFYKDTQGGILMFDVSSRRSFEALDSWLREAAKFga------------------------ 141
gi 42475924  63 VNIFDMAGh--PYFY-EVRNEFYRDAQAALLVFDVCDRNSYETLESWLLEMKQEmgrnn--------------------- 118
gi 70834739  81 VNFWDMSGc--EEYL-EIRNEFYRATEGVLLVYDVTDAESFFALNQWVKEMEAHvntkgndty----------------- 140
gi 42491767  95 VDFFDLSGd--PNYL-EVRNEFYTGVDAVLVIFDVTDKQSFESLSRWMKEAKQYgva----------------------- 148
gi 19387544  65 INFFDLSGl--DEYQ-DIRKEFYSDTQGLLLIFDMSNRDSFTALEKYYGEFIKSstgag--------------------- 120
gi 74831325  58 INFFDLSG---DEDYkEIRNPFFADAQGVVLVCDLTDRQSFQNLPKWEKMMTDNgld----------------------- 111
gi 45478200 171 VSILLNTVvicQSRL-AVRNEFYKDTQGVILVYDVGQKDSFDALDSWLAEMKQElgphg--------------------- 228
gi 68124554 151 VNFWDGSGd--GDYR-EILNEFYEAAQGVLLMYDARNAQSFAALQSWWEELTVYcqgmpaasggggggsgsgggggsnpa 227
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    ## #                               
gi 56789664 124 --------------------------------idnldnIVFAVCANKIDStk--------hRCVDESEGRLWSESKg-fL 162
gi 32997031 145 ----------------------------tlgvnpvkkpAMVVVCANKVDNevdg----gkkRVVSEADGRRWAEAHg-yK 191
gi 42475926 123 ----------------------------------nmdnIVFVVCANKIDCtk--------hRSVDESEGRLWAESRg-fL 159
gi 42491777 123 ----------------------------------nmenIIFVVCANKVDLtk--------rRVVDESEGRLWAESRg-fH 159
gi 21706512 123 ----------------------------------nmenIIFVVCANKIDCtk--------hRCVDESEGRLWAESKg-fL 159
gi 42491781 142 ------------------------------------gkFPCILCGNKVDRp----------RVVSEDEAKAYALSKg-fE 174
gi 42475924 119 ----------------------------------kmdnIVFFVCANKIDKg---------kRMVETLEAQLWAEEKg-fK 154
gi 70834739 141 -----------------------------varvdasvpCKVVVCANKIDEvsegg--grkkRAVSSETGRQWAKEHd-yK 188
gi 42491767 149 -----------------------------------sesTTLIVVGNKIDQl---------gRSVSEQQGISFASENn-aI 183
gi 19387544 121 ---------------------------------agagsVSIYVIGTKADLp----------AAVSVDEATTYASRLk-gV 156
gi 74831325 112 -----------------------------------lkqAAVYVVGNKSDL-----------KNKEVDSGDILAYAKk--K 143
gi 45478200 229 ----------------------------------nmenIVFVVCANKIDCsk--------hRCIDESEGRLWAESRg-fL 265
gi 68124554 228 lagnaaaagaggkrasasnivtsgtaagkavgrtdgraPIVVLCANKVDDtavpgaaaprpRAVSEEQGRAWAREHgcaA 307
                       250       260
                ....*....|....*....|....*...
Feature 1           ###                     
gi 56789664 163 YFETSAQs---GEGINEMFQAFYSSIVD 187
gi 32997031 192 YFETSACt---GLHVTEMLETLFNDVVA 216
gi 42475926 160 YFETSAQt---GEGINEMFQTFYSAIID 184
gi 42491777 160 YFETSAQs---GEGINEMFQSFFSSITD 184
gi 21706512 160 YFETSAQt---GEGINEMFQTFYISIVD 184
gi 42491781 175 YFETSACt---GVSITEAFHSLFRTVVK 199
gi 42475924 155 YFETSAAt---GDGITELFQALFSTLVE 179
gi 70834739 189 YFETSACt---GAGVEEALETLFKDVVA 213
gi 42491767 184 YFETSAQt---GASVDDLFDYLLRETCK 208
gi 19387544 157 FFKVSSCt---GDGVAPALNTIFKQLFS 181
gi 74831325 144 GFEGMICsaagGENVNEVNSNQNCSYLR 171
gi 45478200 266 YFETSAQt---GEGINEMFQTFYMSIVD 290
gi 68124554 308 YYETSASt---GQNVKEVIEDLVVWMVA 332

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