Conserved Protein Domain Family
Rab20

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cd04126: Rab20 
Rab GTPase family 20 (Rab20)
Rab20 is one of several Rab proteins that appear to be restricted in expression to the apical domain of murine polarized epithelial cells. It is expressed on the apical side of polarized kidney tubule and intestinal epithelial cells, and in non-polarized cells. It also localizes to vesico-tubular structures below the apical brush border of renal proximal tubule cells and in the apical region of duodenal epithelial cells. Rab20 has also been shown to colocalize with vacuolar H+-ATPases (V-ATPases) in mouse kidney cells, suggesting a role in the regulation of V-ATPase traffic in specific portions of the nephron. It was also shown to be one of several proteins whose expression is upregulated in human myelodysplastic syndrome (MDS) patients. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins.
Statistics
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PSSM-Id: 133326
View PSSM: cd04126
Aligned: 4 rows
Threshold Bit Score: 351.902
Threshold Setting Gi: 9965335
Created: 7-Feb-2006
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:Based on sequene similarity with other Rab isoforms.
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #######         #    ##                     #                           
gi 8923401    6 SKIVLLGDMNVGKTSLLQRYMERRFPDTVSTVGGAFYLKQWrSYNISIWDTAGREQFHGLGSMYCRGAAAIILTYDVNHR 85
gi 47226955  12 VKVVLLGDMNVGKTSLLHRYMERKFKETISTVGGAFYLKQWgPYNISIWDTAGREQFHGLGSMYCRGAAAVIFIYDVTNW 91
gi 49119003   6 LKVVLLGDMNVGKTSLLHRYMERRFQDTVSTVGGAFYLKQWgPYNISIWDTAGREQFHGLGSMYCRAASAVILTYDVSNM 85
gi 9965335   11 LKVIVMGDFNVGKTSFIGRYIEGEFKQHDATIGAAFFLKQWgPYNIAIWDTAGAERFTGLSSFYCKNAGAAILAFDMSIV 90
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  ## #                                                 
gi 8923401   86 QSLVELEDRFLGLTDTASKDCLFAIVGNKVDlteegalagqekeec---------------------spnmdagdrvspr 144
gi 47226955  92 QSLAELEERFLSLTDTANHDCIYAVVGNKADltdpkaqqlqdpeaasetrtegplegsepqvfsacptppaspaslsglm 171
gi 49119003  86 QSLLELEDRFLGLTDTASDDCIFAVVGNKIDltedynsdsdie----------------------------gerprtssk 137
gi 9965335   91 STFESLWARFIPLLEVANEDCLKIVVGMKLDalg---------------------------------------------e 125
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             ###                                       
gi 8923401  145 apKQVQLEDAVALykkilkykmldeqdvpaaeqMCFETSAKTGYNVDLLFETLFDLVVPMILqqraerpsh---tvdiss 221
gi 47226955 172 lhKQVSPEDAKAFyeriirykglegksslpadkMCFETSAKSGYNVDALFEALFNLVLPFILrkrnenqvsptvdleecr 251
gi 49119003 138 irRQVNLEDAFALykrimkykmldenvvpaaekMCFETSAKTGYNVDVLFEGVFNMVIPLIVkkkasgld----etvnla 213
gi 9965335  126 dqREVTVQEGKKFareindki----dlsklpsdPYFETSSKTGHNVTEVFEYIFRYCLPLSEaqlqaanr-----qgtvn 196
                       250
                ....*....|..
Feature 1                   
gi 8923401  222 hkPPKRTRSGCC 233
gi 47226955 252 ggSSKRSKSSCC 263
gi 49119003 214 qsKPNKSKSRCC 225
gi 9965335  197 lpNGKQSQKKCC 208

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