1GA8


Conserved Protein Domain Family
GT8_A4GalT_like

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cd04194: GT8_A4GalT_like 
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A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface.
The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
Statistics
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PSSM-Id: 133037
View PSSM: cd04194
Aligned: 88 rows
Threshold Bit Score: 150.828
Threshold Setting Gi: 42518148
Created: 31-Oct-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Ligand bindingmetal binding
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:Ligand binding site
Evidence:
  • Structure:1GA8_A: Neisseria Lgtc with bound Mn and donor sugar analog UDP-2-deoxy-2-fluorogalactose
    View structure with Cn3D
  • Structure:1GA8_A: Neisseria Lgtc with bound sugar acceptor analog 4'-deoxylactose
    View structure with Cn3D
  • Comment:Donor and acceptor binding seems to be cooperative; in some glycosyltransferases the nucleotide-sugar bind first, and in others, the acceptor

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             ### ##                                                                     
1GA8_A         1 XDIVFAADdNYAAYLCVAAKSVEAAHpd--------------------tEIRFHVLDAg--iSEANRAAVAAnlrg---- 54
gi 2313238     5 IPIVIAFDnHYAMPAGVSLYSMLACAktehpqs---------qndseklFYKIHCLVDn--lSLENQSKLKEtlapf--s 71
gi 2314591    11 IPIFMSFDkNYALGASVSLYSLLSHAsrhtsmidfsplnqsnkllganiVYKIHCLVKg--vTLEQQNKLLKtiapf--k 86
gi 78223537   10 IPVFFAFDnNYVIPAAVAFHSLLANVnv-------------------syKYHFIVLHEd--iSEENRDLLAQvvslf--- 65
gi 56708299    4 IPIVFTFDkNIILGGAVTIKSLIDHAnp-------------------dtCYDIYVYHPn--iNKKSISAFNSmiek---- 58
gi 120603432   4 VPIVFTFDaNYRLPASVALQSLFENAkd-------------------stYYHVYLVCEg--lSRGDKDAIESicpe---- 58
gi 29340185    6 VPLVIAFTpDYFIPAATCLYSIITSMqa-------------------egELHVICLLSee-lPERLKLKIQLige----- 60
gi 57505203    2 LHICIGVSaEYVKYSAVLINSIVKATqkpfdlkpyen--nlsftkdlkeGFCFHIFTEykseDTEKIALLAHklsei--- 76
gi 55824645    9 FHIVLNANeNYIKYAAVLMTSIIQKTdlnksmse-------fcnfdtdeGYVFHILSDh--iSESMKVRISNlekql--n 77
gi 56783473    2 YHIVFNSSnEYIENLSVLMYSIIINTnks-----------------ntkKYCFHILSSn--iNDNTCKKLTLlekelssi 62
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                      #  #                 ###          
1GA8_A        55 gggnIRFIDVNPEdfagfpl------------------nirhisITTYARLKLGEYia-dcDKVLYLDIDVLVRDSLTPL 115
gi 2313238    72 afssLEFLDISTPnlhatpiepsaidkineaflqlniyaktrfsKMVMCRLFLASLfp-qyDKIIMFDADTLFLNDVSES 150
gi 2314591    87 kfasLEFIHINSLdhsiesyln-------------kscskryggLLVLCRLLLASLfp-nySKIISIDADTVFLGDVASA 152
gi 78223537   66 snasVEFRDMGESfkneweni----------------kgkghytKECLYKLVPMLEfp-qyDKIIWSDVDVVFKDDISDV 128
gi 56708299   59 tkhsISFHNVDESifkdvpi------------------dtrrgwIITFYRLLIPKLlp-qyDKVIYSDVDVLFQSDMSEV 119
gi 120603432  59 kngrVEWIDVDNElfssap-------------------ssenwpKIVYARILLPLLlp--fDKVIYSDVDVVFCSDLAEI 117
gi 29340185   61 --grTCYSFVNLQgklqhiy------------------idqkytEAASYRLLLPDLlp-eyKKVIYIDCDIIVRNDLVQL 119
gi 57505203   77 yptkCLIHTMNNQdfqdfsy------------------pfwcqnAAMFYKIKVADIlk-dvDKCLFIGADLFALGDVRDL 137
gi 55824645   78 diypCKIVLHILNddefkgm------------------lkwrgnYLAYYRIKMASVlpqnlKICLYLDCDMLCFGDLREL 139
gi 56783473   63 ypseIKIYHINDNlfydyni------------------pkhegsYNAYLRLMLASIlskdiKKCLYLDVDMLVLGDISEL 124
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        #                                          ###                  
1GA8_A       116 Wdtdl--gdnWLGASIDlfverqegyk--------------------qkigxadgeyYFNAGVLLINLKkWRRHDIFKXS 173
gi 2313238   151 Ffipl--dgyYFGAAKDfasdkspkhfqivrekdprqafslyehylnesdmqiiyesNYNAGFLVVNLKlWRADHLEERL 228
gi 2314591   153 YfaldndptkSLGMVRDtfshlsfeafcdfckrack--nfkidfsrfspdelkrihqGFNMGFLVAHLDrWRQDGFEKTA 230
gi 78223537  129 Ffmls--eenYIAGVRVcgkldkyyenmn-----------------mpaeiksilknGIGAGILVYNLKkMREDNIYDDI 189
gi 56708299  120 Yntdl-tsyeWAGVIAEkhqqnmvqhk---------------------yfkennnsyIYWPGFMVMNTKlMRENNFISRC 177
gi 120603432 118 Fqiev-dgceWAGVAAElvafqegvarc------------------hnvhceyqnelIYMSGFMVMNLRlMREKDTVGRC 178
gi 29340185  120 Yhsid-lgmnYLAAVFEasmdfqldhl---------------------ktigcnpneYINSGFLIMNLElMRKDNMVEKF 177
gi 57505203  138 Faldl--kdnLIAAALDtynfdgylrkakak-------------nsdeelvfndaknYINNDMMLINLKeWRKQNLQAKY 202
gi 55824645  140 Lsvdi--nnyQAAVCLDgnnhkknkkvffsl-------------kgrekykfsniekYFNSGFILVNLDrWRRDNIENKS 204
gi 56783473  125 Fdldl--kdkVFAAVFIlkhpwpnlnsk------------------dsseifyiygsHFNSGLMLINLDaWREKNIESRS 184
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      ##                      #                                         
1GA8_A       174 SEWVeqykdvxqyqDQDILNGLFKGg-VCYANSRFNFXPTNyafxanwfasrht---------------------dplyr 231
gi 2313238   229 LNLThqkgqcvfypEQDLLTLACYQk-VLILPYIYNTHPFManqkrf--------------------------------- 274
gi 2314591   231 LEFLktrgkdlfypEQCLVNMVFWEr-ILELPIYYNCYSDFfkeh----------------------------------- 274
gi 78223537  190 MIALqgmssivvqpEQDILNIVLKDk-IDYIPLRYCFCTYMynlfkdrhkmklkvkgnlfnylfkgyrknlgfdtiysek 268
gi 56708299  178 FDTMhefntrlkfrDLDVLNLTCRK--IKSLPFKYVTLQSIyylntiqeapeyifl-----------------keiysdn 238
gi 120603432 179 LNNIskfgsrlkmyDLEILNMSSDN--IARIDFSYCVLENVffaknvseakeypwl-----------------rglyrvs 239
gi 29340185  178 IEASkvdy--lefpDQDVLNQLCKDr-ILALPPYYNSIRTFylpqykkfflqk------------------------yte 230
gi 57505203  203 IDYLnkyd---lagDLDVFPLVCAPk-IHILSSKYNFILGYytresfglentlkdesd-------------kpvwnftkv 265
gi 55824645  205 IDFLkkfk--tlypDQDALNFALND--TLLLPNRWNFSLGYfvaflknsqeilflnqtk------------yphlnytkt 268
gi 56783473  185 LSFIknyyv-pyavDEYVLNAILSKddIFSLKLEWNFLIGFrrlylnndlffnkeegdk------------ykiicyske 251
                        330       340
                 ....*....|....*....|....*
Feature 1                    # ##    #    
1GA8_A       232 drtntvxpVAVSHYCGp--AKPWHR 254
gi 2313238   275 --ipdkkeIVMLHFYFv--GKPWVL 295
gi 2314591   275 ----ypknIIMLHFIK---YKPWRS 292
gi 78223537  269 elleafesPAIIHYATs--TKPWNT 291
gi 56708299  239 elldaknnPAIIHYAGs-pGKPWRM 262
gi 120603432 240 eleaarsaPRIIHFAGs-dTKVWER 263
gi 29340185  231 qdwlevhrHGTVHYTG---AKPWNQ 252
gi 57505203  266 eleqiqkdLRLVHFCHy-vYKPWMS 289
gi 55824645  269 efenevknIKIAHFILd-pFKPWDA 292
gi 56783473  252 efekafkkIKILHYTYlymPKPWEN 276

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