2AH5,3SD7


Conserved Protein Domain Family
HAD_5NT

?
cd04302: HAD_5NT 
Click on image for an interactive view with Cn3D
haloacid dehalogenase (HAD)-like 5'-nucleotidases similar to the Pseudomonas aeruginosa PA0065
5'-nucleotidases dephosphorylate nucleoside 5'-monophosphates to nucleosides and inorganic phosphate. Purified Pseudomonas aeruginosa PA0065 displayed high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
?
PSSM-Id: 319798
View PSSM: cd04302
Aligned: 20 rows
Threshold Bit Score: 279.861
Threshold Setting Gi: 78101348
Created: 3-May-2006
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####                                                                       
2AH5_A         6 AIFFDLDGTLVDSsiGIHNAFTYTFKELGvpspdaKTIRGf-XGPPLESSFatcls---kdqisEAVQIYRSYYKAKGiy 81
gi 23465321    8 VVLLDLDGTLTKSdgGIIASVVKTFEELGrpvpddAELHRf-IGPAIIESLrrnhvp--edeldRAVTIYRSYYADRAvf 84
gi 27375609    6 SVFLDLDGTLVDSqpGIAASCSAALRVLGhd---pGKAPDiaIGPPLEDILrtllqaygddridEAVVAYRQHYGESGll 82
gi 125719137   6 HIFFDLDGTLVDSskGIQESFEYSFKQLGkec-peESIIKsfMGPPLEVSFasvle---esqvpEAINYYRSFYKEKGiw 81
gi 163848356  11 AILFDLDGTLTDPftGIARSVNYALQRLGhpllsdDELRRw-IGPPLHASFeqlldd--prladQAVAHYRQRYWETGly 87
gi 169157101  20 CILFDLDGTITDSapGITAQLAKTLVFMGlpvpgpAQLLEy-VGPPILDSFrdlagm-dddaqqRALAHYREGYAGGGvf 97
gi 116494298   3 NLFFDFDGTVADSevGIVAGLKYMVQDRGlrpldnSAYTQf-IGPALIDMLpkvwpelddievkRAIDAFHHYYEEEGly 81
gi 110826511  81 LVIFDLDGTLTDSarGIVSSFRHALNHIGapvpegDLATHi-VGPPMHETLramgl---gesaeEAIVAYRADYSARGwa 156
gi 215919121  34 YLLFDLDGTLTDPkeGITRSVQYALEQMNvacpsmDELEWt-IGPPLIDAFalllntkdkkqlqKAINFYRERYVDRCai 112
gi 21232616    5 TLFFDLDGTLVDSepGIVGSIVHAFDEVGqprpspQTLRAw-IGPPLRDSFserfpe-dpdlvqRALAAYRARYDAVGwt 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               ##                                    #  
2AH5_A        82 --------------eAQLFPQIIDLLEELSSs-yPLYITTTKDtSTAQDXAKNLEIHHFFDGIYGSSPea-----phKAD 141
gi 23465321   85 ddpnepgnkvpgrlvNVVFPGIREQLLKLRAdgyYLALASCKPeYQCVPICEHFHLTELLDGIYGASRdns---rldKDQ 161
gi 27375609   83 --------------gSEPYPGISGALQELRQaglRIYLATSKReVFARRILENLNLAAYFDGIHGSVPggk---ldhKPE 145
gi 125719137  82 --------------gVRLYEGIPELLTQLKEagyQIYVTTSKNqPTAQKLLANLAISEQFDDIFGSLPds-----fhKAD 142
gi 163848356  88 --------------eNTVFPGIPELLSDLKAagiRLFVATSKIrPPTERILAHFGLQPFFEAIAAVDPddr---dgnKAM 150
gi 169157101  98 --------------dSSVYVGVPEVLRAIHAagiPLSLATSKPeSQARRVLDHYGLTELFTEICGASEdev---rsaKAD 160
gi 116494298  82 --------------qVELYPNFLDTMAKLKQqdnQLYIASAKPeTMLHRLVKHFSLDQYFAGAYGASDdeet--rvtKTD 145
gi 110826511 157 --------------mNSLFDGIGPLLADLRTagvRLAVATSKAePTARRILRHFGIEQHFEVIAGASTdgsrgskvdVLA 222
gi 215919121 113 --------------eNKPYDGIHETLETLVNhgyQLYLATSKPwAYAGKILDHFNLRDYFTAVHGSELdgt---rdyKEE 175
gi 21232616   83 --------------eLSVFDGIGDVVTGLHRaghRLAVVTSKNeRYARRIVEHLPFGVCFEDVIGASEdga---rrfKPD 145
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                              ##  ##                                           
2AH5_A       142 VIHQALQthql---apeqAIIIGDTKFDXLGAREtGIQKLAITWGFGEQadllnyQPDYIAHKPLEVLAYF 209
gi 23465321  162 VIRYCFDkigfdaaagdkAVMIGDRYTDIDGAHAcNLDAIGCRWGYAPAgemeehGAYEIIEKPEQIEEAV 232
gi 27375609  146 LLAHILSecgi---aashSIMIGDRHHDIAGAHAvKMRGLGVLWGYGNRdeleaaGADQLVERPADLARTV 213
gi 125719137 143 VLRRALQtlda---npeeTIIIGDTKFDIIGGKEvGISTLGVLWGFGSQrellenGADLLSNSPKHILKIL 210
gi 163848356 151 VIGTLLPtlga---drawTVMVGDTIFDIHGARAhRMPCIAVTYGYGEYrdlylaAPLALASTVGTLRELL 218
gi 169157101 161 VIEEALRrlraagvdlgnAVMVGDREHDVLGAAAhGIPTVMVGWGYGSPae--aaGTIAVVDTAAELEARL 229
gi 116494298 146 ILTYALKdsqv---aldqSVMIGDRANDMIGGKAnHVQLLGVTYGFGSAkelkeaGAKVLVDQPADIPAGL 213
gi 110826511 223 HALAQLRplp------erLVMVGDRSHDVDGAAAhGIDTVVVGWGYGRAdfi-dkTSTTVVTHAATIDELR 286
gi 215919121 176 LIGYVLDtqki---sntqSLMIGDRRYDILGAKHnNVRSIGVTYGYGSLeeiqeaGPDAVCDYHRLILPTL 243
gi 21232616  146 LIAEALRrlqi---dktgCVMIGDRRMDIDGANHhGIHSIGVLWGFGDEaelraaGAGALAHTPAELSGLI 213

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap