2W4M,2HOQ,3QNM,4KNW,2GFH


Conserved Protein Domain Family
HAD_Neu5Ac-Pase_like

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cd04305: HAD_Neu5Ac-Pase_like 
Click on image for an interactive view with Cn3D
human N-acetylneuraminate-9-phosphate phosphatase, Escherichia coli house-cleaning phosphatase YjjG, and related phosphatases
N-acetylneuraminate-9- phosphatase (Neu5Ac-9-Pase; E.C. 3.1.3.29) catalyzes the dephosphorylation of N-acylneuraminate 9-phosphate during the synthesis of N-acetylneuraminate; Escherichia coli nucleotide phosphatase YjjG has a broad pyrimidine nucleotide activity spectrum and functions as an in vivo house-cleaning phosphatase for noncanonical pyrimidine nucleotides. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319800
View PSSM: cd04305
Aligned: 31 rows
Threshold Bit Score: 130.74
Threshold Setting Gi: 380033490
Created: 13-Apr-2006
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####                                                                       
2W4M_A        30 AVFFDLDNTLIdtagasrrgmlevikllqskyhykeeaeiicdkvqvklskecfhpyn---------------------- 87
2HOQ_A         4 VIFFDLDDTLVdtsklaeiarknaienmirhglpvdfetayselielikeyg---------------------------- 55
4KNW_B        10 AVFFDLDNTLIdtagasrrgmlevikllqskyhykeeaeiicdkvqvklskecfhpyn---------------------- 67
2GFH_A        20 AVFFDLDNTLIdtagasrrgmlevikllqskyhykeeaeiicdkvqvklskecfhpys---------------------- 77
gi 46250196    8 AVFFDLDNTLIdtsgagkkaieevvkvlteenqykedeahiicnkfqaklgcetldss---------------------- 65
gi 38258666    4 AVFFDIDDTLYdtsgfaklarkaalnvmidaglpltqeeaykllreiisekg---------------------------- 55
gi 47209535    4 AIIFDLDNTLIdtrragevaiqktgdllktalalddatiavicdkfkqkllgercdpsa--------------------- 62
gi 38258811    4 AVLFDVDDTLYpssklaeearrnairamieagletdlseeelyrelqevvkey--------------------------- 56
gi 38258704    4 GILFDLDDTLYnssefveiarreavksmidaglnidfeeamnilnkiikdkg---------------------------- 55
gi 251757402   3 AVFFDLDDTLLwdeksvrttfaetclqaekkyglapeefeaavreaarelymsyetypytvmiginpfeglwsnfsepis 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                               ##       
2W4M_A        88 -----tcitdlrtshweeaiqetkggaanrklaeecyflwkstrlqhmtlaEDVKAMLTELrk-eVRLLLLTNGdrQTQR 161
2HOQ_A        56 ----------snfpyhfdyllrrldlpynpkwisagviayhntkfaylrevPGARKVLIRLkelgYELGIITDGnpVKQW 125
4KNW_B        68 -----tcitdlrtshweeaiqetkggaanrklaeecyflwkstrlqhmtlaEDVKAMLTELrk-eVRLLLLTNGdrQTQR 141
2GFH_A        78 -----tcitdvrtshweeaiqetkggadnrklaeecyflwkstrlqhmilaDDVKAMLTELrk-eVRLLLLTNGdrQTQR 151
gi 46250196   66 -----kmtiddlrvrhweeamlevrqgdhkevasdcytmwktrrlqlltmsQSTKDMLCELrk-sTRLVLLTNGvrQVQR 139
gi 38258666   56 ----------snydrhfnvltktvfgeekpllialgmityhnvkfallrpfPNTTSTLIDLkskgYRLGVISNGitIKQW 125
gi 47209535   63 ----crsveevrvahwqesiqevrgsssasalasqcyrlwkstrlevlslpPERSELLKQLra-sYKLLLLTNGlaEVQR 137
gi 38258811   57 ---------gsnhprhfdlllrrigadpepklvaaavvayhdtkfaylkpyPDVIPTLMQLremgFKLGAVTSGlaVKQW 127
gi 38258704   56 ----------snygkhfddlvkavlgkydpkiittgiityhnvkvallrpyPHTIKTLMELkamgLKLGVITDGltIKQW 125
gi 251757402  83 egfqklnkivpeyrrnawtnglkalgiddpaygeylgeffaaerrkrpfvyDETFAVLDQLkg-kYELLLLTNGdpSLQK 161
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
Feature 1                                 #                       ##   ##                
2W4M_A       162 EKIEACACqs-yFDAVVVGGEQREEKPAPSIFYYCCNLLgvqpgdCVMVGDTLETDIQGGLNAGlkATVWIN 232
2HOQ_A       126 EKILRLELdd-fFEHVIISDFEGVKKPHPKIFKKALKAFnvkpeeALMVGDRLYSDIYGAKRVGm-KTVWFR 195
4KNW_B       142 EKIEACACqs-yFDAVVVGGEQREEKPAPSIFYYCCNLLgvqpgdCVMVGDTLETDIQGGLNAGlkATVWIN 212
2GFH_A       152 EKIEACACqs-yFDAIVIGGEQKEEKPAPSIFYHCCDLLgvqpgdCVMVGDTLETDIQGGLNAGlkATVWIN 222
gi 46250196  140 EKIESCGAqq-fFDAVVVGGEHAEEKPAPSIFYHCCDLIgvlpgdCVMVGDNLDTDIQGGLNAGlkATIWIN 210
gi 38258666  126 EKLIRLGIhh-fFDEVVTSDEVGFEKPNIRIFEEALRRMgckperSVMVGNKFNEDILGATNAGm-SAILVN 195
gi 47209535  138 EKVEAVGCee-fFDAVVIGGEHAEQKPSPSIFTWCFDALgvraedCVVVGDDLDADIQGGFNAGvrATVWIS 208
gi 38258811  128 EKLIRLGIhh-fFHEVVISEEIGVEKPNPKIFIEAARRLgvkpeeAVYVGDRLDKDIRGANRAGm-VTVRIR 197
gi 38258704  126 EKLIRLGIhp-fFDDVITSEEFGLGKPHLEFFKYGLKRMglkaeeTVYVGDRVDKDIKPAKELGm-ITVRIL 195
gi 251757402 162 EKLAGVPElapyFNEIVISGAFGKGKPDVSIFEHCLKLMniekddAIMVGDNLNTDILGASRAGi-KTVWIN 232

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