Conserved Protein Domain Family
LbH_gamma_CA_like

?
cd04645: LbH_gamma_CA_like 
Click on image for an interactive view with Cn3D
Gamma carbonic anhydrase-like: This family is composed of gamma carbonic anhydrase (CA), Ferripyochelin Binding Protein (FBP), E. coli paaY protein, and similar proteins. CAs are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism, involving the nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Gamma CAs are trimeric enzymes with left-handed parallel beta helix (LbH) structural domain.
Statistics
?
PSSM-Id: 100051
View PSSM: cd04645
Aligned: 150 rows
Threshold Bit Score: 128.298
Threshold Setting Gi: 1827571
Created: 19-Sep-2006
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
putative metaltrimer
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:putative metal binding site [ion binding site]
Evidence:
  • Comment:Based on the active site/zinc binding site of gamma carbonic anhydrase (1THJ).
  • Comment:Zinc ions are bound at the interface of neighboring subunits of the trimer. The trimer contains three zinc binding sites.
  • Structure:1THJ; Methanosarcina thermophila gamma carbonic anhydrase trimer binds three zinc molecules.
    View structure with Cn3D
  • Citation:PMID 8665839
  • Structure:1V67_A; Pyrococcus Horikoshii Ferripyochelin Binding Protein binds zinc. The binding of zinc to only one subunit is shown in this structure.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                               #                        
1THJ_A        28 VIDPTAYIDPEASVIGEVTIGANVMVSPMASIRSDEGm-PIFVGDRSNVQDGVVLHAletineegepiednivevdgkey 106
gi 89301492  366 SINDSEFIAPNSVVIGDVITKEGSSIWYGATLRGELG--PIEIGKQTVIQDLVNIQSgkqn------------------q 425
gi 146161176  58 TFYHSTFIAPNSSLIGAVYLGQNTVVGYGSTLRGDNH--AIRVGHNTVIGDKVAISNvatlaa-------------gipv 122
gi 68124341   57 WTQDACFIAPNAFVVGNVVLGHDTAIFYHSVLRNYHTksATILGDHTVVMDRATLMGq---------------------- 114
gi 158271535  65 YYPVDVFVAPNAVVCGDVDIYGGASVFFGAVLRGDLN--KIRLGNRSAILDRAVVHAaravpt-------------glna 129
gi 25091504   71 KVAVDAYVAPNVVLAGQVTVWDGSSVWNGAVLRGDLN--KITVGFCSNVQERCVVHAawsspt-------------glpa 135
gi 109676401  51 KLRPSVFVAPNASLIGNVSVMDESSIWYGAVVRGDQS--PVDIGGKSSIGDRSVVLSasvnpt-------------gfaa 115
gi 89292088   76 QIGYQSYIAPNSTVIGEVTIGNETTVWYNSVIRGDVN--AVQIGNNVSIGENVVIHTagslpt-------------gqpa 140
gi 115292273  52 RVAQGAFIAPNAAVIGNVDIEPRTSIWYGAVIRGDQS--NIFIGGESSIGDRSVVQSstvnpt-------------gfsa 116
gi 145492395  48 EIGSQHFIASTATVIGDVELASQCVVWYGAVLRGDLN--GIRILNRVIIGERSVLHTaaslpn-------------gmpa 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #    #                                                               
1THJ_A       107 avYIGNNVSLAHQSQVHGpAAVGDdTFIGMQAFVF-KSKVGnNCVLEPRSAAIGv-TIPDGRYIPagmvvtsqaeadkLP 184
gi 89301492  426 ktQIGDNVFIGPNSYIQS-SKINDnSFVGMGSTVStGCNLAsNAVVAAGSVVPEntQVPSNQIWAgsp-------aqyLR 497
gi 146161176 123 stNIGNHVNIGAGCVLQS-CVVDDnVTVGHNTVILeGSVLErGSVIAPNSLVPAgrLIPSGQLWAgsp-------vryVR 194
gi 68124341  115 -iRVGQGTYIGAGATLDC-CEVHDnVYVGPGASIAlGAVVEnGAIVAAGSAVPKdaRVYAGELWAgnp-------aqkIA 185
gi 158271535 130 atLIGEKVTVEPYAVLRS-CRVEPkVIIGARSVVCeGAVVEsESILAPNSVVPParRIPSGELWGgsp-------akfIR 201
gi 25091504  136 qtLIDRYVTVGAYSLLRS-CTIEPeCIIGQHSILMeGSLVEtRSILEAGSVLPPgrRIPSGELWGgnp-------arfIR 207
gi 109676401 116 ktSIGDWVTVGQGCVLRG-CTVDNfAVVGDGCVIGeGALVEtHGVLEAGSVLPAggLVPRGEVHGgnp-------aafVR 187
gi 89292088  141 svDIGHYVIIGSKSTIYS-CTIQDeVVIGQGCVILeGARIEkGAMIAANSVVPPgrLIPAGTLWAgnp-------ctfVR 212
gi 115292273 117 rtCIGDWVKIGQGCVLRA-CTIEDyCQIGDGCIVQeGALIEnGAMLEPGSVVPQgaRVPAGEVYAgnp-------atfVR 188
gi 145492395 113 vlSIGNNVMVQNDCTLYS-CTIGDnCFIGYRSIILeGAKLEdGAVLAPGTVVPPgrLIPSNQLWAgnp-------aqyVR 184
                        170       180
                 ....*....|....*....|....
Feature 1                                
1THJ_A       185 EVTDdyayshtNEAVVYVNVHLAE 208
gi 89301492  498 DITPeer--qvLQEHHQECVQLAR 519
gi 146161176 195 DLKEeei--klNLEQTEQNLSIGK 216
gi 68124341  186 DVSPaqs--seVQHMVHDQIQVGK 207
gi 158271535 202 KLTDher-drvLDDVSTHYHNLAT 224
gi 25091504  208 TLTNeet--leIPKLAVAINHLSG 229
gi 109676401 188 KLEKdei--aaIEKKAEDVSMSAK 209
gi 89292088  213 NLTKsel--atNIDHAKKQLHLAQ 234
gi 115292273 189 KLSKeei--eeFGEYAEEVCDLAA 210
gi 145492395 185 DIEDkdl--sqLSYVIGNQFAIAR 206

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap