Conserved Protein Domain Family
LbH_MAT_like

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cd04647: LbH_MAT_like 
Click on image for an interactive view with Cn3D
Maltose O-acyltransferase (MAT)-like: This family is composed of maltose O-acetyltransferase, galactoside O-acetyltransferase (GAT), xenobiotic acyltransferase (XAT) and similar proteins. MAT and GAT catalyze the CoA-dependent acetylation of the 6-hydroxyl group of their respective sugar substrates. MAT acetylates maltose and glucose exclusively while GAT specifically acetylates galactopyranosides. XAT catalyzes the CoA-dependent acetylation of a variety of hydroxyl-bearing acceptors such as chloramphenicol and streptogramin, among others. XATs are implicated in inactivating xenobiotics leading to xenobiotic resistance in patients. Members of this family contain a a left-handed parallel beta-helix (LbH) domain with at least 5 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). They are trimeric in their active form.
Statistics
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PSSM-Id: 100053
View PSSM: cd04647
Aligned: 191 rows
Threshold Bit Score: 79.8079
Threshold Setting Gi: 118711634
Created: 22-Nov-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of E. coli galactoside acetyltransferase and Enterococcus faecium streptogramin acetyltransferase Vat(D) bound to CoA and their respective substrates.
  • Comment:Active acetyltransferases in this family are trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Structure:1KRV; E. coli Galactoside Acetyltransferase trimer binds three CoA and substrate 4-nitrophenylgalactoside molecules; defined at 4A contacts.
    View structure with Cn3D
  • Structure:1KHR_A; Enterococcus faecium streptogramin acetyltransferase Vat(D) binds CoA and substrate Virginiamycin; defined at 4A contacts. Shown are two active sites within the trimer.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                # #                                             # #                     
1MR9_Z        29 NVEVGeYSYYDskngetfdkQILyhypilndkLKIGKFCSIGpgVTIIXng---aNHRXdgstypfnlfgngw------- 98
1KRV_C        75 NIHIGrNFYANfn------lTIVdd-----ytVTIGDNVLIApnVTLSVt-----GHPVhhelrkn-------------- 124
gi 68551263   68 YLDIGdHVHIGgg------cHVSgt-----ggVTISDFCGLSqgVRIYSv-----TDDYtgysltnatv----------- 120
gi 9654661    37 RISIGsNVRIDdy------vTISagv----ggVEIGSHVHIGvySSLIGa-----GKITledfvgvsgrvsiysssddyt 101
gi 42781097   35 VISVGnHVRIDdf------cILSg-------kITIGSYSHISayTALYGgevgieMHDFanisaktivyavlddfsg--- 98
gi 83309211   60 NFVCGkNVWIGeg------aILDam-----ggLEIGDNSQIGsyVMVWSh----sSHKQairgetgt------------- 111
gi 46200177  143 NLELGdDVVVHr-------yVFLddi----ggIKIGDRTSLSdyVNVYSh-----THHVlaspd---------------- 190
gi 76559289  147 NIEMGdNVVVHnd------vLLDdr-----grLQIGDRVSVAdrSHIHTh-----AHDTvdqsd---------------- 194
gi 104783692 216 GIALGeHVGLGrg------vVIEga-----ggVAIGEHSQLDdqVTLVSs-----SHDHgyrsl---------------- 263
gi 85860438   30 NLKLGyKTDIGaf------tYINak-----ygVTIEDFVQVGshCSIYSv-----STIDdr------------------- 74
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
Feature 1                                    # ##    #           ##    ##        ## #    
1MR9_Z        99 -----ekhxpkldqlpikgDTIIGND-VWIGKdVVIXpgVKIGDgAIVAANSVVVKDIAPyXLAGGNPANEI 164
1KRV_C       125 -------------gemysfPITIGNN-VWIGShVVINpgVTIGDnSVIGAGSIVTKDIPPnVVAAGVPCRVI 182
gi 68551263  121 ---------perfksvkvaPVNLGRH-VLIGSgSVVLpgVTIGEgSAVGALSLVSISLDEwGVYFGSPAKRI 182
gi 9654661   102 gmamsnptvpeeltkvtslPVLIKKH-SILGAgCVVLpkVIVGEgVSVGALSLVNKSLDDwHIYSGNPIQKF 172
gi 42781097   99 --ntlmgptvpnqyrnvktEKVILKKhAIIGAnSILFpnVTIGEgTAVGAMSMVKESLDDwYIYAGIPVRKV 168
gi 83309211  112 -----------srdrieykRTKIGSN-VHIPGpTVIApgVTIGNgVMIAPLSFVNEDLPDrAVFSPAREMRR 171
gi 46200177  191 ---------------vtlkETIIGSG-VRITYhATVLagVRIGDdAMVGTGAVVTKDIPPhAIALGIPARPV 246
gi 76559289  195 ---------------vtnyETILDDD-VRLGYgSMISagCRIGEnAMVGSGATTLGDVPPhHIAAGSPAKSV 250
gi 104783692 264 --------------pwkaaPIRIGKR-CRIGTgALIVgpVSLGDdVVVEPYAVVIRDVPAhSVVKGIIQLKE 320
gi 85860438   75 -----------------qgPVVLKKN-CRIGShSVVMpgVTVGEnAVVGACSFVNADIPEnAVAVGMPARVI 128

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