1HYH,1EZ4,1LDN,1LLC


Conserved Protein Domain Family
HicDH_like

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cd05291: HicDH_like 
Click on image for an interactive view with Cn3D
L-2-hydroxyisocapronate dehydrogenases and some bacterial L-lactate dehydrogenases
L-2-hydroxyisocapronate dehydrogenase (HicDH) catalyzes the conversion of a variety of 2-oxo carboxylic acids with medium-sized aliphatic or aromatic side chains. This subfamily is composed of HicDHs and some bacterial L-lactate dehydrogenases (LDH). LDHs catalyze the last step of glycolysis in which pyruvate is converted to L-lactate. Bacterial LDHs can be non-allosteric or may be activated by an allosteric effector such as fructose-1,6-bisphosphate. Members of this subfamily with known structures such as the HicDH of Lactobacillus confusus, the non-allosteric LDH of Lactobacillus pentosus, and the allosteric LDH of Bacillus stearothermophilus, show that they exist as homotetramers. The HicDH-like subfamily is part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133427
View PSSM: cd05291
Aligned: 66 rows
Threshold Bit Score: 372.185
Threshold Setting Gi: 71851261
Created: 14-Dec-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:1HYH_C; Weissella confusa Hydroxyisocaproate Dehydrogenase contacts to NAD at 3.5A
    View structure with Cn3D
  • Citation:PMID 7643402
  • Structure:1LDN_A; Geobacillus stearothermophilus allosteric LDH binds NAD; defined at 3.5A contacts.
    View structure with Cn3D
  • Citation:PMID 1731077

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ###                    ##                                           ##  
1HYH_C         2 RKIGIIGLGNVGAAVAHGLIAQGVaDDYVFIDANeAKVKADQIDFQDAMANle--aHGNIVINDWAALADADVVISTL-- 77
gi 53987585   27 IKIALIGAGNVGNSFLYAAMNQGLaSEYGIIDINpDFADGNAFDFEDASASlp--fPISVSRYEYKDLKDADFIVITA-- 102
gi 17367620    2 KKVVLIGTGNVGVTVVYTMITKGIdAEYVLIDINtEFAKGHAMDMSDAIALns-ttGSKIRTGTYADAKGADLLIVAA-- 78
gi 33860171    2 KKIAVIGCGFVGSTYILDLLQQGVqADYLLVDKNtNLADGHVRDLRDSKSLks-hnGSTFNVGTYDDLKDADVVAITAsi 80
gi 71851261    2 KKIIVIGMGNVGTTFVNIALARGLqANFVFVDKNeEICQAHVHDFQDMIALmprnnSTFKVGTLLQEAKDADIVIVTAs- 80
gi 81697084    6 KRVAMVGAGLVGVSVLYSCMNRGLaEQYGIIDINdKLSVGHSLDFEDASAAnn--hNFSVGKIEYSDLKDYDVVVITA-- 81
gi 68446250    4 HKIVLIGSGYVGSAFAHAIVAKGLvDEMAIIDIDeDKAKADVWDLNHATPFgd--nFVNVHVGQYEDFKDADIVVICA-- 79
gi 49036093    6 KKVVIVGAGAVGSTYAHNLVVDDLaDEIAIINTNkSKASANSLDLLHALPYln-aaPKNIYAADYSDVSDADIVVLSA-- 82
gi 38257848    6 KKVVLVGDGSVGSSYAFAMVTQGIaDEFVIIDIAkDKVEADVKDLNHGALYss--sPVTVKAGEYEDCKDADLVVITA-- 81
gi 116494184   5 GNIILIGDGAIGSSYAFNCLTTGVgQSLGIIDVNeKRVQGDVEDLSDALPYt---sQKNIYAASYEDCKYADIIVITA-- 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ##                                            ###                      #       
1HYH_C        78 -GNIKL---QQDNPTgdrfAELKFTSSMVQSVGTNLKESGFHGVLVVISNPVDVITALFQHVTGFPAHKVIGTGTLLDTA 153
gi 53987585  103 -GRPQK---PGETRL----ELVADNIRIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFSDQKVIGSGTVLDTA 174
gi 17367620   79 -GRPQK---QGETRL----EMIADNSKIMKDIALEIKKSGFNGFTIVISNPVDILATVFQKVTNFPKEKVMSSGTFLDTS 150
gi 33860171   81 pTVPTAdgeVFTDRL----QLMTANVKILNEIALELKRVGFKGLSIIPTNPCDVMAGVYQKVTGFDPHKIISTGCQLETM 156
gi 71851261   81 -IPANK---DFSDRL----KLAEANSKLMKSFGDQLKEAKFKGIVIVAANPCDVMASVFTYASNLPFKKVISTGTLLDSA 152
gi 81697084   82 -GRPQK---PGETRL----EMVADNAKIMSNIAKNIKKSGFKGVSIVVANPVDVMTFIYQHETGFDKNRVISSGTSLDSA 153
gi 68446250   80 -SAKLA---KGETRL----KLLEDNVDIFVPMIQRIVDSGFDGYFVLPSNPVDIMSYVVKRVSNFPKNKIIGSGTSLDTA 151
gi 49036093   83 -NAPSAtfgKNPDRL----QLLENKVEMIRDITRKTMDAGFDGIFLVASNPVDVLAQVVAEVSGLPKHRVIGTGTLLETS 157
gi 38257848   82 -GAPQK---PGETRL----QLVEKNTKIMKSIVTSVMDSGFDGFFLIAANPVDILTRYVKEVTGLPAERVIGSGTVLDSA 153
gi 116494184  80 -GIAQK---PGQTRL----QLLAINAKIMKEITHNIMASGFNGFILVASNPVDVLAELVLQESGLPRNQVLGSGTALDSA 151
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                #                                                       
1HYH_C       154 RMQRAVGEAFDLDPRSVSGYNLGEHGNSQFVAWSTVRVMGQPIvtla----dagdIDLAAIEEEARKGGFTVLNGKGYTS 229
gi 53987585  175 RLQFAIAKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFcayskltgidssNYEKELEYPVSRRAYEIINRKRATF 254
gi 17367620  151 RFRKFLSEKTGVPTNSVHGFVIGEHGDKSVVVFSRMQIGFSRLddflks-kalteDDLKWISEKTYKEAYEIINRKRSTY 229
gi 33860171  157 RTRKMISEALGVNSDSVEGFVVGEHGSGAIVPWSVFRVGNVPMkqliae-gkikeEYVKDIFSRVVKEAFEIIKFKKATY 235
gi 71851261  153 RFKKFIAQKFNVNADSVQASILGEHGSSAIPLWSTVKIGDATLenllks-kklksNELNQILKQTIDEAFYIFSRKGNTQ 231
gi 81697084  154 RLRFEISKKLKVHPKSVQAFVLGEHGDSSVSVYSAATVSGKSFneivke-rgiskKELEDMHTTVYKKAYEIINRKGSTY 232
gi 68446250  152 RFQFFLSREFDVAPNQVYAPIIGEHGDSQVAVWSHAQIAGEPVldllps-ntnleAFKTSISNRTTQIGYDIYVRKGTTN 230
gi 49036093  158 RMRQIVAEKLQINPKSIHGYVLAEHGKSSFAAWSNVTVGAIPLttwlkkypnpefPTFDEIDQEIREVGLDIFMQKGNTS 237
gi 38257848  154 RFRYLISKELGVTSSSVHASIIGEHGDSELAVWSQANVGGISVydtlke-etgsdAKANEIYINTRDAAYDIIQAKGSTY 232
gi 116494184 152 RLRSEIGLRYNVDARIVHGYIMGEHGDSEFPVWDYTNIGGKPIldwip--kdrqdKDLPDISERVKTAAYGIIEKKGATF 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1HYH_C       230 YGVATSAIRIAKAVMADAHAELVVSNRRDDm----gMYLSYPAIIGRDGVLa-ETTLDLTTDEQEKLLQSRDYIQQRFDE 304
gi 53987585  255 YGIGAAIAKIVSNIIKDTKNIMIAGANLRGeygfhgVNIGVPVVLGANGIEk-IIEISLNDKEKEKFAKSVAIIDKIYQD 333
gi 17367620  230 FGIGASVAEMAESVLYNQRRIFPIGIYLDEskpgggIYISRPAILGENGWEe-VKNYDLSPAEQKAFDESAINLKKHWDD 308
gi 33860171  236 FGIAESMSLITRAYIYNLNTVLGVGVQLDDkyvasgIYFTVPAVVGKHGWKl-HSKLQLSQEEQAAFDKSALNIQKVTKD 314
gi 71851261  232 FGIGTSLYEITDAILNNKKQVMNIGVKLPAnyknagIYISIPVIVGKNGYEylSVKPNFNNNELKQFEASTSHMAKVHKD 311
gi 81697084  233 FGIGSTVAELVEAILTDSHAIFGVGVYLTGqygvkdLYIGVPTVLGSKGVVe-VINFNLTKEEQEKFVSSATILKGNIQK 311
gi 68446250  231 FGISLSLVRIVEAILFNKNIIMNVSSYVEGeyglsdVYIGVPTVINRNGADr-IIELALSKEELSQLHHSADIILDYQRQ 309
gi 49036093  238 YGIAASLARLTRAIFRNESVILPVSAYLTGeygqfdLYTGSPAIIDRTGVRa-VLELELTQEEQEKFKASTVLLKENFDS 316
gi 38257848  233 YGIALALLRISKALLNNENSILTVSSQLNGqygfndVYLGLPTLINQNGAVk-IYETPLNDNELQLLEKSVKTLEDTYDS 311
gi 116494184 230 YGIAASLTRLTSAFLNDDRAAFAMSVHLEGeyglsgVSIGVPVILGANGLEr-IIELDLNPEDHKRLADSAAILKENLKK 308

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