1I0Z,1I10,5LDH,1V6A,2LDX,2V65,2I6T


Conserved Protein Domain Family
LDH_1

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cd05293: LDH_1 
Click on image for an interactive view with Cn3D
A subgroup of L-lactate dehydrogenases
L-lactate dehydrogenases (LDH) are tetrameric enzymes catalyzing the last step of glycolysis in which pyruvate is converted to L-lactate. This subgroup is composed of eukaryotic LDHs. Vertebrate LDHs are non-allosteric. This is in contrast to some bacterial LDHs that are activated by an allosteric effector such as fructose-1,6-bisphosphate. LDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133429
View PSSM: cd05293
Aligned: 28 rows
Threshold Bit Score: 398.901
Threshold Setting Gi: 76779532
Created: 14-Dec-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   ###                    # #   #                                     ##
1I0Z_A        18 VPNNKITVVGVGQVGMACAISILGksLADELALVDVLEd-KLKGEMMDLQHGSlflqTPKIVADKDYSVTANSKIVVVTA 96
2I6T_A        12 KTVNKITVVGGGELGIACTLAISAkgIADRLVLLDLSE--GTKGATMDLEIFN----LPNVEISKDLSASAHSKVVIFTV 85
gi 76779532  136 PSHAKVTVIGGGDMALACLLAVSAkgTAGKLVLLDPTDgePAGGATADLEIFS----LPNVQVTKDFSAIAGSAIVIVTV 211
gi 45550422   46 PRISKISVVGAGQVGTAISAMLLLrnLTKNLVILDINYe-LAKAEALDFQHASaflsDARVVPCGDSTNSKDSDVVIITA 124
gi 195381589 388 QPGNKVSVIGAGAVGMGCAIALLAkgITNNIALYDLKKd-LCAAECMDLEHGSlflnNCNIDHCTSVECTKDSRVVVVTA 466
gi 198460131  40 KPSLKITVVGSGQVGAAVAAFLLArnLTKHLVILDVKYd-LATAEALDFSHGSaflgNPIVEACGDGNRTKNSDVIIITA 118
gi 194757710  56 KPGFKITVVGAGQVGLACCAFLLQrrLAKELVIMDIQYe-RAQAEARDFNHAAvfltDPKITACGDGVNTKDSDVVIVTA 134
gi 195431973  38 KISPKISVVGAGQVGISICAFLIQrnLARHLVIHDIRYd-WAKAEALDLLHAScwlkNPHIEACGDGSHTKDSDIVIVTA 116
gi 118091283 233 GCFNKVTVVGAGDLGIACVLAVVAkgIADKVVLLDLSEg-AAKGGAMDLEIFA----LPNVEISKDFSASTDSKVVVLTV 307
gi 195388180  17 EPADKISVIGTGQVGLSCCAFLIDrrLANHLVMVDLRQe-WVKAEALDFLHVSsllaSPKIETCTDASCTAGSKYVIITV 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###                                     # #                      #   #          
1I0Z_A        97 GVRQQEGESRl-NLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGlpkHRVIGSGCNLDSARFRYLMA 175
2I6T_A        86 NSLGSSQSYL--DVVQSNVDMFRALVPALGHYSQHSVLLVASQPVEIMTYVTWKLSTfpaNRVIGIGCNLDSQRLQYIIT 163
gi 76779532  212 NAWSNSQSYV--GVLQSNVELLRRIVPTVVHHCPKCLLLVASQPVEIMTYVTWKLSGfphTRVLGIGCNLDSGRFHHVIE 289
gi 45550422  125 GARPSGKDRSrlAAMQKTVEILKKAVPKLVELSPNATFIIISNPADVMTYAVQRITNlpkHRCFTTGCHLDTVRFRNLIA 204
gi 195381589 467 GVRCNQNESRl-KVAQKSAGIIKEIVPELVKQSPKGVFIIVSNPADVMAWVARKVTKlpyERCFSPGCHLDTARFRMFIA 545
gi 198460131 119 GARPSGKTRSrlDVMHKTVVILKSVVPKLVELSPKAIFIIICNPADVMTFAVQRIGNlekHRCFTTGCHLDTARFRYLIA 198
gi 194757710 135 GVRPGKKSRL--EVMHDTIKIFKELVPKLVSFSPNAIYLVVSNPADVMTHAFQKIGKipkHRCFSTGCHLDTARFRYFIA 212
gi 195431973 117 GARPVGKDRSrlLVMKKTVEILKLAMPKLIELSPKAVFVIVSNPADVMTYAIQRICKlekHRCFTTGCSLDSARFRFLIA 196
gi 118091283 308 NSLGNAQTYL--DVIQSNVDLFRGIIPAISHYSQNSVLLVASQPVEIMTYVSWKLSAfpkSKVIGVGGNLDTERFQYILT 385
gi 195388180  96 GTRPAGKSRL--DIAKESLELLSKLVPKLVESSPDATYVISSNPADVMTWAVRKMSGlpkERCISCGCHLDSLRFRYFLA 173
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #                                                      #   #   
1I0Z_A       176 EKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVslqelnpemgtdndsenwKEVHKMVVESAYEVIKLKGYTNWAIGLS 255
2I6T_A       164 NVLKAQTSGKEVWVIGEQGEDKVLTWSGQEEVVS------------------HTSQVQLSNRAMELLRVKGQRSWSVGLS 225
gi 76779532  290 KLVNSEEGAQDAWIIGEQSENKVAVWGDPDSSAKnqisg---------klypKIFQEQLTSRALEMLKGKGQRSWSVGLS 360
gi 45550422  205 NRLRLPPSQVHGYVIGEHGASAVPVWSSVSIAGIrlndvvknlacgddpenwAKINKQVTTGGLAVAKTKGYTNWAIALT 284
gi 195381589 546 QLVRVSTRSVHGFVLGEHGDSSVPLWSSVTVGGTrlqnmlptigtdkdpmrwSNVHESVVDSAFKVIAGKGYTNWAIGLT 625
gi 198460131 199 KRLKVPTSAVNAYIIGEHGSSAVPVWSSVTVGGIrlsdvvkdlgtdkdqekwSDLMDGILNAGVHVSKAKGYTNWAVGLT 278
gi 194757710 213 QRLKVPTTAVKGFVIGEHGDSAVPVWSGVQVAGAclkdivknpgcgpdpekwAEINKEVTCGGSSVSSVKGYTNWAVALT 292
gi 195431973 197 QRLKVPTTEVEGYVIGEHGNSAVPVWSSVTVGGVplvkvvrdlgtsmdtenwNNLFKDVTQAGASVGNIKGYTNWAIALS 276
gi 118091283 386 KLLQAEALGKDAWIVGEQGEDKVPSWTSSKSVTNetet-----------taaHNSREKVANRAVEVLKGKGQRSWSVGLS 454
gi 195388180 174 QRLGVAASEVNAFIVGEHGDSSVPVWSGVTVGGIallrllpnigrdqdeekwQKVHEDVVKAGSSVSKIKGYTNWGVALA 253
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                                                    
1I0Z_A       256 VADLIESMLKNLSRIHPVSTMVKgmygieNEVFLSLPCILNARGLTsVINQKLKd--DEVAQLKKSADTLWDIQKD 329
2I6T_A       226 VADMVDSIVNNKKKVHSVSALAKgyydinSEVFLSLPCILGTNGVSeVIKTTLKe-dTVTEKLQSSASSIHSLQQQ 300
gi 76779532  361 VADITHTLILNNGKVHSVSTLSKglfgvqEEVFLSIPCVLGSVGVM-GTVQTLQedvQIWETLQRSAAAIEAVQQQ 435
gi 45550422  285 CADIVQAMSGGKGKIACVGTDMKglngiqDNVVLSLPCLVTAGGIShVFELPLTd--VEQSKLLASANILLEAQCS 358
gi 195381589 626 VTDVVSAIFENSNRIMSLSTNAQgmcgidDEVFLSLPCIVNQCGLYgVIHPQLSg--WEERRLKRSADVLLDAQKT 699
gi 198460131 279 ASDVVKCMVENTGQVCCVGTDVKglyglkDSVVLSVPCRVTASGIShILELPLNe--DERKKLLKSADTLLEAQCS 352
gi 194757710 293 AVDVVAAIAGPYGRIMSIGTDMKgihdieENVVLSMPCMVTPSGIShIFEIPLSh--GEFEMLHKSAKILFKAQCE 366
gi 195431973 277 AVNVVEAMLENSGRILCLGTDMQgmnsigQQVVLSLPCKVTSTGIShVLQLPLTa--LEQEKLHKSAEVLMKAQCS 350
gi 118091283 455 VADLTDSILKDKRKVHSVSTLAKgfcninSEVFLSLPCILGTSGVVeTLTLEEDp--AVQEKLQSSAGSIHDLQQQ 528
gi 195388180 254 IVDIIQAMMTNSGRILSVGSDIQglmgvnDSVVMSLPCILGTHGVQkVIELPLTe--FEMNMFEKSKEVLMKAQCA 327

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