1OU5,1MIW,1MIY


Conserved Protein Domain Family
NT_ClassII-CCAase

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cd05398: NT_ClassII-CCAase 
Click on image for an interactive view with Cn3D
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes.
CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.
Statistics
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PSSM-Id: 143388
View PSSM: cd05398
Aligned: 93 rows
Threshold Bit Score: 102.286
Threshold Setting Gi: 15893603
Created: 18-May-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site annotation includes NTP- and metal-binding residues, as well as residues involved in primer tRNA binding in Aquifex aeolicus Aase.
  • Structure:1MIY_A, Bacillus stearothermophilus CCA-adding enzyme monomer bound with CTP and Mg2+, contacts determined at 4.5A
    View structure with Cn3D
  • Structure:1MIW_A, Bacillus stearothermophilus CCA-adding enzyme homodimer, bound with ATP and Mg2+, contacts determined at 4.5A.
    View structure with Cn3D
  • Comment:Some members of this group form dimers in solution.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          ##  ##          # #                           
1OU5_A        56 TEGLKSLTELFVKe-------------NHELRIAGGAVRDLLNGV--KPQDIDFATTA-TPTQMKEMFqs--agIRMInn 117
1MIY_A         5 FQEALGIIQQLKQh-------------GYDAYFVGGAVRDLLLGR--PIGDVDIATSA-LPEDVMAIF------PKTIdv 62
gi 153956374   1 MNYIKKFIYDAKDtin---------evEGELYLVGGYIRNKLMNIasQPKDADFVFNGdIHEFISKLQnk---gYKFFpi 68
gi 37522493   15 MPQLTSLLDAIPFapa---------dwPGPLALVGGSVRDALLNRtrVPLDLDFVCPGpVAERARVLArrlgagFAVLda 85
gi 150387946   1 MNKIIACILETGKv------------sNIDIYLVGGSIRDFLLNR--NIKDLDLIIKEdPGRFAEKIAkel-qgSYFVld 65
gi 19703588   10 SDVEIGILNKLNE--------------YGKGYIVGGAIRDILLGL--KPKDVDFTTNL-PYETLKKLFse--ynLKEIgk 70
gi 94266115   11 HSYPAELATALAGac----------gaQRELYIAGGAVRDWLRGC--RSRDLDFTVPAgGIAFARRLAaq--lqASLVil 76
gi 51246762   29 SPDIVTALFAVSRq------------lDAEVWVAGGPVRDYFLGR--SAKDLDLVTGAgAIDFCRRLLrql-geGSLVpl 93
gi 20806638    1 MATIPRLIRKLGRe-------------AYNVYIVGGYIRDRILNK--EAKDYDFVVKG-NAEEIAKLAaek-mgGSFVpf 63
gi 126695498   6 LTDHTLIIDELEKsikfhnlnfilgflPKGSYLVGGYIRDIILGRktEKLDVDIVVPLkAIEIGKKIAen--ieSKFIvl 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #             #    #                              ####  #                   
1OU5_A       118 rgekHGTITARLH-EENFEITTLRidvttdg------rhaeveFTTDWq-KDAERRDLTINSMFLGFD---------GTL 180
1MIY_A        63 g-skHGTVVVVHK-GKAYEVTTFKtdgdyed-----yrrpesvTFVRSleEDLKRRDFTMNAIAMDEY---------GTI 126
gi 153956374  69 k-esVSIYRCIKD-KNKIDVSLMK-------------------GNSIE--EDLENRDFTINALALRFSd--------NKI 117
gi 37522493   86 ---eHEIVRLVLDsGITLDFARRQ-------------------GADLV--SDLARRDYTVNAIAWDVHa--------GEL 133
gi 150387946  66 --qdRGIHRVKVKdGITIDLAKFQ-------------------GEDIL--ADLAKRDYTINAMAYPLEsgwp--ideSQL 120
gi 19703588   71 ---sFGVLRIKIN-DIDYEIAKFRednyeekdglkiipegkkvSFVDDikNDLTRRDFTINAMAYNEV---------EGI 137
gi 94266115   77 d-peHDLARVVWR-DLDLDCSGLRe-----------------kATGMA--EDLRRRDFTINAMAVALEvrdkrcrqqPGL 135
gi 51246762   94 segdEEACRIVYR-HEIVDISSFRa-----------------sASSLE--EDLALRDFTLNAMALPLStlr---gekVDL 150
gi 20806638   64 m-aeKGTYRIVVG-DEILDFTNLR-------------------GKDIY--EDLAHRDFTINAMAIRLTdhf----efEYI 116
gi 126695498  84 d-ekREVVRIFLN-NIDIDIANQV-------------------SSTIE--GDLFSRDFSINSIAFLLDk--------KCL 132
                        170
                 ....*....|..
Feature 1                    
1OU5_A       181 FDYFNGYEDLKN 192
1MIY_A       127 IDPFGGREAIRR 138
gi 153956374 118 IDPFYGRKAIQS 129
gi 37522493  134 FDPFDGRGDLTR 145
gi 150387946 121 IDPYGGQQDIKE 132
gi 19703588  138 VDLYNGQKDIEN 149
gi 94266115  136 LDPTGGRQDLAA 147
gi 51246762  151 IDPFSGRDDLAD 162
gi 20806638  117 IDPFGGLKDIKN 128
gi 126695498 133 LDPLNGLKDLEI 144

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